BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0116.0040.p (449 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 568 e-162 Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 513 e-145 PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 492 e-139 mll2278 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 257 5e-68 mlr9217 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 253 7e-67 >PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 469 Score = 568 bits (1464), Expect = e-162, Method: Compositional matrix adjust. Identities = 266/442 (60%), Positives = 340/442 (76%), Gaps = 1/442 (0%) Query: 1 MNCVAHQSPGLWTHPRDRTRDYNKLSYWIDLAKTLERGRFDGLFLADVLGVYDVYANSPD 60 MNCV HQSPGLW HPRDR+ Y L YW DLA+ LERGRFDGLF+ADVLG+YDV + D Sbjct: 12 MNCVGHQSPGLWAHPRDRSSQYKDLEYWTDLARLLERGRFDGLFIADVLGIYDVLDGNGD 71 Query: 61 AALRNAAQTPANEPLMLIPAMAAVTENLGFGVTSNLSFEPPYPFARRMSTLDHLTGGRVG 120 AA+R AAQ P N+PL LI MAAVT +LGFG+T++LSFE PYPFARR+STLDHLT GR+G Sbjct: 72 AAIRQAAQVPVNDPLALITPMAAVTRHLGFGLTASLSFEHPYPFARRLSTLDHLTKGRIG 131 Query: 121 WNVVTGYLDSAARGAGKDKQTAHDDRYELADEYMELVYKLWEGSWEDDAAIRDVARGIFT 180 WNVVT YL+S AR G+ Q HD RY+ ADEY++++YKL+EGSWED A +RD R IF+ Sbjct: 132 WNVVTSYLESGARNLGQQAQLDHDRRYDYADEYLQVLYKLFEGSWEDGAVLRDRRRRIFS 191 Query: 181 DPSKVHPVHHDGNNYRLNAIHLSEPSPQRTPVLYQAGTSPRGRQFAAEHAECVFMSGPSA 240 DP K+H + H G ++++ IHL EPSPQRTPVLYQAG S RG++FAAEHAECVF++ PS Sbjct: 192 DPGKIHEIRHVGEHFQVPGIHLCEPSPQRTPVLYQAGASSRGKRFAAEHAECVFVAAPSK 251 Query: 241 KVIGPRVSAIRELAAAKGRNPAEILMFSMMCVVVAPTEAEAKAKYAEYRSHISHEGALAL 300 V+ V+ IR+ A GR+P ++L+F++ VV+ T+A+A+AK+ EYR+ +S+EGALAL Sbjct: 252 TVLKQAVADIRQRVAEAGRDPRKVLVFNLQTVVLGETDAKARAKFDEYRAFVSYEGALAL 311 Query: 301 MSGWTGVDFSTYSLDQEVRHVQNDAGRSAMDNVTRADPDRVWTVREVAEHVGIGGAGPVV 360 +SGWTG+DF Y DQ +RH+ +A +SA++ + ADP R WTVRE+A+ VGIGG GP+ Sbjct: 312 ISGWTGIDFGQYRPDQVLRHIHTNAIQSAVEAFSSADPTRTWTVRELADWVGIGGFGPLF 371 Query: 361 VGSPEQVADKIEQWFEDTDVDGLNVAFAISPGDFEDIADMLVPELVKRDRYKREYAKGTL 420 VGSP VAD +++W EDTDVDG N+A+A++ F D+ ++LVPEL KR YK EYA GTL Sbjct: 372 VGSPATVADLLQEWVEDTDVDGFNLAYALAHETFSDVVELLVPELQKRGVYKTEYAPGTL 431 Query: 421 REKLFGAGRARLDTTHPASRYR 442 REKLFG G RL +HPA+ YR Sbjct: 432 REKLFGDG-PRLPDSHPAAGYR 452 >Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Cgl Corynebacterium glutamicum Length = 487 Score = 513 bits (1321), Expect = e-145, Method: Compositional matrix adjust. Identities = 240/443 (54%), Positives = 317/443 (71%), Gaps = 1/443 (0%) Query: 1 MNCVAHQSPGLWTHPRDRTRDYNKLSYWIDLAKTLERGRFDGLFLADVLGVYDVYANSPD 60 MNCVAHQSPGLWTHP+D+ RDYN L YW+ LAKTLE+G FDGLF+ADVLG YDVY +S + Sbjct: 21 MNCVAHQSPGLWTHPKDKARDYNTLDYWVHLAKTLEKGLFDGLFIADVLGTYDVYGSSNE 80 Query: 61 AALRNAAQTPANEPLMLIPAMAAVTENLGFGVTSNLSFEPPYPFARRMSTLDHLTGGRVG 120 AAL + AQ P N+P++L+ AMA T+NLGFG+T+ ++E PYPFARR++TLDHLT GRVG Sbjct: 81 AALSSGAQVPVNDPILLVSAMAYATKNLGFGITAGTAYEHPYPFARRLATLDHLTNGRVG 140 Query: 121 WNVVTGYLDSAARGAGKDKQTAHDDRYELADEYMELVYKLWEGSWEDDAAIRDVARGIFT 180 WNVVTGYL SAA+ G Q HD+RY+ ADEY+E++YKL EGSWEDDA + +FT Sbjct: 141 WNVVTGYLPSAAQNMGDTDQLPHDERYDKADEYLEVIYKLLEGSWEDDAVQNNTETSVFT 200 Query: 181 DPSKVHPVHHDGNNYRLNAIHLSEPSPQRTPVLYQAGTSPRGRQFAAEHAECVFMSGPSA 240 D SKVH ++H G + + I ++EPS QRTPV+YQAG SPRG +FA E+AE VF++ + Sbjct: 201 DSSKVHAINHHGKYFDVPGIAITEPSVQRTPVIYQAGASPRGLKFAGENAEAVFINSSTV 260 Query: 241 KVIGPRVSAIRELAAAKGRNPAEILMFSMMCVVVAPTEAEAKAKYAEYRSHISHEGALAL 300 + I V+ IR A A GR+P + +F+M ++ TEA+A+AK EY +I G L L Sbjct: 261 EAITKTVAKIRAAAVAAGRDPHAVKIFAMQTIITGETEADAQAKLEEYSRYIDPVGGLTL 320 Query: 301 MSGWTGVDFSTYSLDQEVRHVQNDAGRSAMDNVTRADPDRVWTVREVAEHVGIGGAGPVV 360 MSGWTG D S Y LD+ + +++++A +S ++ + WTVR++ E GIGG GPV+ Sbjct: 321 MSGWTGADLSQYDLDEPITNIESNAIQSTAATISNGTGEGAWTVRKLGEATGIGGFGPVL 380 Query: 361 VGSPEQVADKIEQWFEDTDVDGLNVAFAISPGDFEDIADMLVPELVKRDRYKREYAKGTL 420 VGS VA ++ + + +DVDG N+A+AI+PG FED+ D +VPEL K RYK EYA G+L Sbjct: 381 VGSGANVAAELARIQDLSDVDGFNLAYAITPGTFEDVVDFVVPELQKLSRYKTEYAPGSL 440 Query: 421 REKLFGAGRARLDTTHPASRYRV 443 R KL G G RLD TH + YR+ Sbjct: 441 RNKLLGKGD-RLDDTHRGASYRL 462 >PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 462 Score = 492 bits (1266), Expect = e-139, Method: Compositional matrix adjust. Identities = 235/449 (52%), Positives = 306/449 (68%), Gaps = 2/449 (0%) Query: 1 MNCVAHQSPGLWTHPRDRTRDYNKLSYWIDLAKTLERGRFDGLFLADVLGVYDVYANSPD 60 MNCV H + GLWTHPRDR+ DY KL +W +LA+ LERG FDGLF+AD+LGVYDVY + Sbjct: 13 MNCVGHINHGLWTHPRDRSSDYRKLRHWTELARLLERGLFDGLFIADILGVYDVYQGGIE 72 Query: 61 AALRNAAQTPANEPLMLIPAMAAVTENLGFGVTSNLSFEPPYPFARRMSTLDHLTGGRVG 120 R A Q P N+PLML+ AMA VTE+LGFGVT+NL++E PYPFARR+STLDHL+ GR+G Sbjct: 73 VTAREAVQLPVNDPLMLVSAMAGVTEHLGFGVTANLTYEAPYPFARRLSTLDHLSDGRIG 132 Query: 121 WNVVTGYLDSAARGAGKDKQTAHDDRYELADEYMELVYKLWEGSWEDDAAIRDVARGIFT 180 WN+VTGYL+SAA+ G+ Q HD RY+ ADEY+E++YKLWEGSWEDDA + D R ++ Sbjct: 133 WNIVTGYLESAAKAMGQSAQIEHDRRYDRADEYLEVLYKLWEGSWEDDAVLADRQRRVYA 192 Query: 181 DPSKVHPVHHDGNNYRLNAIHLSEPSPQRTPVLYQAGTSPRGRQFAAEHAECVFMSGPSA 240 P K+H + H G Y++ HL+EPSPQRTPVL+QAG+S RG FAA HAECVF+SG + Sbjct: 193 RPEKIHKIRHHGEFYQVEGYHLAEPSPQRTPVLFQAGSSERGLAFAARHAECVFVSGQTR 252 Query: 241 KVIGPRVSAIRELAAAKGRNPAEILMFSMMCVVVAPTEAEAKAKYAEYRSHISHEGALAL 300 + V IR GR +I +F + V+VA T AEA+ K+AEYR + S E LA Sbjct: 253 EATRQLVQRIRAAVVGAGRRAEDIRIFMGINVIVAATAAEARDKHAEYRRYASPEAGLAH 312 Query: 301 MSGWTGVDFSTYSLDQEVRHVQNDAGRSAMDNVTRADPDRVWTVREVAEHVGIGGAGPVV 360 + +G+D S Y LD+ +R+ +N+A S + N+T A D TVR++ + + +GG + Sbjct: 313 FASSSGIDLSRYGLDEPIRYEKNNAIESVVKNLTVARTDS--TVRKLLDQLALGGRYVTL 370 Query: 361 VGSPEQVADKIEQWFEDTDVDGLNVAFAISPGDFEDIADMLVPELVKRDRYKREYAKGTL 420 VG P QVAD +E W DT +DG N+ + P + D D++VPEL R YK YA GTL Sbjct: 371 VGDPRQVADDLEGWIADTGLDGFNLTRTVEPESYADFIDLVVPELQARGSYKTAYAPGTL 430 Query: 421 REKLFGAGRARLDTTHPASRYRVKSAAAE 449 REKLFG GRARL H + +R AA + Sbjct: 431 REKLFGDGRARLQAEHAGAAFRQPPAARQ 459 >mll2278 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Mlo Mesorhizobium loti Length = 450 Score = 257 bits (656), Expect = 5e-68, Method: Compositional matrix adjust. Identities = 150/438 (34%), Positives = 245/438 (55%), Gaps = 27/438 (6%) Query: 12 WTHPRDRTRDYNKLSYWIDLAKTLERGRFDGLFLADVLGVYDVYANSPDAALRNAAQTPA 71 W HPR R+ Y+ +L + ER R D +F+AD + ++D ++++ A Sbjct: 21 WRHPRAPGREVLDFDYYRELVQAAERARIDTIFVADHVSIWD--------SVKSGVAHYA 72 Query: 72 N---EPLMLIPAMAAVTENLGFGVTSNLSFEPPYPFARRMSTLDHLTGGRVGWNVVTGYL 128 N EPL L+ A+AAVT+++G T++ S+ PY AR ++LDH++ GR WNVVT + Sbjct: 73 NARLEPLTLLSALAAVTKHIGLITTASSSYSEPYNVARLFASLDHISKGRASWNVVTSAM 132 Query: 129 DSAARGAGKDKQTAHDDRYELADEYMELVYKLWEGSWEDDAAIRDVARGIFTDPSKVHPV 188 D AR G+D H RYE A E++++V LW+ SWED+A + D G F DP KVHP+ Sbjct: 133 DEEARNFGRDGNIEHAFRYERAGEFLDIVKALWD-SWEDEALLIDKETGYFADPDKVHPI 191 Query: 189 HHDGNNYRLNA-IHLSEPSPQRTPVLYQAGTSPRGRQFAAEHAECVFMSGPSAKVIGPRV 247 H G ++++ +++S P PQ P++ QAG+S G+ FA HA+ F + + Sbjct: 192 DHKGKHFKVRGPLNVSRP-PQGHPLIVQAGSSEDGKNFATAHADAHFAIYSTREDGIKYR 250 Query: 248 SAIRELAAAKGRNPAEILMFSMMCVVVAPTEAEAKAKYAEYRSHISHEGALALMSGWTGV 307 I E A GR P + + +VA +EAE + K ++ + + + L+S W+G+ Sbjct: 251 QDINERLARHGRRPESFKILPGILPIVAASEAEGRDKQEYLQTLLPDQVGIDLLSSWSGI 310 Query: 308 DFSTYSLDQEVRHVQNDA----GRSAMDNVTRADPDRVWTVREVAEHVGIGGAGPVVVGS 363 D S Y D + + +++ GR++++ V + + ++R++A + G+ P V G+ Sbjct: 311 DLSAYPPDGPLPPLPDESTFNGGRTSLNRVKQWSKQNL-SLRDIARKLANSGSVPTVAGT 369 Query: 364 PEQVADKIEQWFEDTDVDGLNVAFAISPGDFEDIADMLVPELVKRDRYKREYAKGTLREK 423 P+Q+AD++E WF DG N+ F + P D+ + A+ +VPEL +R EYA GTLR++ Sbjct: 370 PKQIADQLEDWFVSGAADGFNLMFPLLPEDWVNFAEQVVPELQRRGLVPTEYAPGTLRDR 429 Query: 424 LFGAGRARLDTTHPASRY 441 L G AR PA+R+ Sbjct: 430 L---GLAR-----PANRF 439 >mlr9217 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Mlo Mesorhizobium loti Length = 426 Score = 253 bits (646), Expect = 7e-67, Method: Compositional matrix adjust. Identities = 144/415 (34%), Positives = 216/415 (52%), Gaps = 9/415 (2%) Query: 11 LWTHPRDRTRDYNKLSYWIDLAKTLERGRFDGLFLADVLGVYDVYANSPDAALRNAAQTP 70 +W HP+ R + ++W L +TLE GRFDGLF ADVL ++ + + Q Sbjct: 18 MWRHPQTENR-FLDPAFWETLGRTLEAGRFDGLFFADVLSFHN------ETTMTRGGQMS 70 Query: 71 ANEPLMLIPAMAAVTENLGFGVTSNLSFEPPYPFARRMSTLDHLTGGRVGWNVVTGYLDS 130 +P+ L+ MA T +G GVT + +F+ PY AR +S+LD L+GGR+ WN+VT + D Sbjct: 71 LLDPVPLVAMMARATSRIGLGVTLSTTFQDPYAIARVLSSLDVLSGGRIAWNIVTSFSDL 130 Query: 131 AARGAGKDKQTAHDDRYELADEYMELVYKLWEGSWEDDAAIRDVARGIFTDPSKVHPVHH 190 A+ G++ +RYE A+E +++ LW G W D A + D G F D +KV Sbjct: 131 DAQIFGEEALLPRSERYERAEEVLKVCLDLWTG-WADGALVLDKESGDFIDEAKVRRTEI 189 Query: 191 DGNNYRLNAIHLSEPSPQRTPVLYQAGTSPRGRQFAAEHAECVFMSGPSAKVIGPRVSAI 250 G + PSPQR PV+ QAG+SPRGR FAA HAE VF S + + + Sbjct: 190 AGKYVGARGVFTVPPSPQRHPVIMQAGSSPRGRDFAASHAELVFTLQHSLPDMQEFYAEM 249 Query: 251 RELAAAKGRNPAEILMFSMMCVVVAPTEAEAKAKYAEYRSHISHEGALALMSGWTGVDFS 310 ++ GRNP + + + +V T + A+ K A + + MS TG+D S Sbjct: 250 KDRVGRAGRNPDDCAILVSVDPIVGETNSLAREKQAYINDLVDPHVGIPFMSDHTGLDLS 309 Query: 311 TYSLDQEVRHVQNDAG-RSAMDNVTRADPDRVWTVREVAEHVGIGGAGPVVVGSPEQVAD 369 Y +D+ + + + G R + D + + T+ E A+H + P VVG+PE VAD Sbjct: 310 QYPVDKPIIDIPAETGSRGSFDIILQGTKANNLTLGEAAKHFAVSELCPQVVGTPETVAD 369 Query: 370 KIEQWFEDTDVDGLNVAFAISPGDFEDIADMLVPELVKRDRYKREYAKGTLREKL 424 +++ +FE+ DG V PG FE +VP L KR +++EY TLRE + Sbjct: 370 QLQDFFENKGCDGFIVTPTEMPGSFEAFTRSVVPILQKRGLFRKEYPGSTLRETI 424