BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0115.0183.p (392 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA5232 [M] COG0845 Membrane-fusion protein Pae Pseudomonas aerug... 261 1e-69 AGl433 [M] COG0845 Membrane-fusion protein Atu Agrobacterium tum... 249 6e-66 BMEI0653 [M] COG0845 Membrane-fusion protein Bme Brucella melite... 233 8e-61 yhiI [M] COG0845 Membrane-fusion protein Eco Escherichia coli K12 230 4e-60 ECs4360 [M] COG0845 Membrane-fusion protein Ecs Escherichia coli... 229 8e-60 >PA5232 [M] COG0845 Membrane-fusion protein Pae Pseudomonas aeruginosa Length = 357 Score = 261 bits (668), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 146/340 (42%), Positives = 214/340 (62%), Gaps = 29/340 (8%) Query: 79 VVLGGVVGTGYYVWKKAHPP-LPVGISLGNGRIEADEIDIATKFAGRVSELLVDIGDIVA 137 +++ ++G G VW+ + P L G + GNGRIEA E+D+A K GRV+E+ VD GD V Sbjct: 13 LIVAALLGVGVLVWQVSRPTGLGEGFASGNGRIEATEVDVAAKLPGRVAEIKVDEGDFVK 72 Query: 138 PGQVLARMDTRDIEQSL------------SKAEAQTRQALRAVEE--AQANLAQQQTTKT 183 G+++ARMDT+ +E L +K A + A R E+ AQA +AQ+Q T Sbjct: 73 AGEIVARMDTQVLEAQLAQAQAQVRQAENAKLTATSLVAQRESEKSTAQAVVAQRQAELT 132 Query: 184 LAEQEMGRTNSLLK-NGWTTQEIADQR-------------KQSLDAAIAGLNAAQARVLV 229 A++ RT +L+K N Q++ D R + + +A A + A +++V+ Sbjct: 133 AAQKRFTRTEALVKRNALPQQQLDDDRATLQSAQAALSAARSQVISAQAAIEAGRSQVIE 192 Query: 230 AQHAREAAQHDAGFYRVQIADNTLVAPKAGRIQYRLANIGEVLPGGGKVFTMLDLSYVYM 289 AQ A EAA+ + I D+ L AP+ GR+QYR+A GEVLP GGK+ M+DL+ VYM Sbjct: 193 AQSAIEAAKASVARLQADIDDSLLKAPRNGRVQYRVAQPGEVLPAGGKLLNMVDLADVYM 252 Query: 290 DIYLPTLEAGKVKVGADARIVLDAYPDHPIPAKVSFVASHAQFTPKTVETQTERDKLMFR 349 +LP+++AG+V +G + R+V+DA PD+ IPAKVS+VAS AQFTPKTVET ER+KLMFR Sbjct: 253 TFFLPSMQAGRVGLGQEVRLVIDAVPDYVIPAKVSYVASVAQFTPKTVETANEREKLMFR 312 Query: 350 IRTRIDEERLLKHAESVRSGLPGMAYVRWDAKLAWPKNLE 389 ++ R+D L K+ V++G+PGMAY+R D ++ WP NL+ Sbjct: 313 VKARLDPALLEKYITYVKTGVPGMAYLRLDPEVEWPANLQ 352 >AGl433 [M] COG0845 Membrane-fusion protein Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 354 Score = 249 bits (637), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 152/344 (44%), Positives = 207/344 (60%), Gaps = 31/344 (9%) Query: 77 LLVVLGGVVGT--GYYVWKKAHPP-LPVGISLGNGRIEADEIDIATKFAGRVSELLVDIG 133 LL+ L VV Y+ W K LP G NGRIEA EIDI+TK AGR+ +++V G Sbjct: 6 LLIGLAVVVLAIAAYFAWSKLTAEGLPSGFVASNGRIEAVEIDISTKTAGRLQDIMVREG 65 Query: 134 DIVAPGQVLARMDTRDIEQSLSKAEAQTRQALRAVEEAQANLAQQQTTKTLA-------- 185 D V GQVLA+MDT +E +AEAQ R+A A++ A + +AQ++ KT A Sbjct: 66 DFVKAGQVLAQMDTAQLEAKKRQAEAQLRRAKIAIDTAHSLVAQREAEKTSALAVVEQRK 125 Query: 186 ------EQEMGRTNSLLKNGWTTQEIADQRKQSLDAAIAGLNAA--------------QA 225 + R+ LL +Q+I D + + +A A L +A +A Sbjct: 126 AQLDSASKTYARSRQLLTGNAVSQQIVDDSEATEQSARATLASAQASLAASDAAINAAKA 185 Query: 226 RVLVAQHAREAAQHDAGFYRVQIADNTLVAPKAGRIQYRLANIGEVLPGGGKVFTMLDLS 285 +++ A+ A +AAQ D I D TL +P+ GR+QYR+A GEVL GG+V ++DL Sbjct: 186 QIIDAEAAVDAAQADIESIETDIVDATLKSPRDGRVQYRIAQPGEVLSAGGRVLNLVDLG 245 Query: 286 YVYMDIYLPTLEAGKVKVGADARIVLDAYPDHPIPAKVSFVASHAQFTPKTVETQTERDK 345 VYM +LPT EAG+ +G+D R++LDA P + IPAKVSFVA AQFTPKTVET+ ER K Sbjct: 246 DVYMTFFLPTAEAGRTSMGSDVRLILDAAPQYTIPAKVSFVADVAQFTPKTVETEEERQK 305 Query: 346 LMFRIRTRIDEERLLKHAESVRSGLPGMAYVRWDAKLAWPKNLE 389 L FR+R +I +E L K+ + V++GLPGMAYVR D + AWP+NLE Sbjct: 306 LTFRVRAQIPQELLQKYIQYVKTGLPGMAYVRLDPQAAWPENLE 349 >BMEI0653 [M] COG0845 Membrane-fusion protein Bme Brucella melitensis Length = 331 Score = 233 bits (593), Expect = 8e-61, Method: Compositional matrix adjust. Identities = 139/318 (43%), Positives = 194/318 (61%), Gaps = 28/318 (8%) Query: 99 LPVGISLGNGRIEADEIDIATKFAGRVSELLVDIGDIVAPGQVLARMDTRDIEQSLSKAE 158 LP GI+ GNGR+EA EIDI+TK GR+ E+ + G V G VLARMDT +E +A+ Sbjct: 9 LPDGIASGNGRVEAVEIDISTKSPGRIREIFANEGSFVKAGDVLARMDTDQLESQYRQAK 68 Query: 159 AQTRQALRAVEEAQ--------------ANLAQQQTTKTLAEQEMGRTNSLLKNGWTTQE 204 AQ R+A ++ AQ A +AQ++ A++ + R+ L ++ +Q+ Sbjct: 69 AQLRRAEIGIDTAQSLVTQRQAEHAAAEATVAQREAQLDAAQRRLARSRQLSESRTVSQQ 128 Query: 205 IADQRKQS--------------LDAAIAGLNAAQARVLVAQHAREAAQHDAGFYRVQIAD 250 + D + + L A A + AA+A+V+ AQ + EAA+ + D Sbjct: 129 VLDDDRATAQGAEAAVGAAKAQLAATDAAIGAAKAQVVDAQASVEAAKAAIAAIEADLRD 188 Query: 251 NTLVAPKAGRIQYRLANIGEVLPGGGKVFTMLDLSYVYMDIYLPTLEAGKVKVGADARIV 310 TL APK GR+QYR+A GEVL GG+V ++DLS V M +LPT +AG+V +GADARIV Sbjct: 189 ATLTAPKPGRVQYRVAQPGEVLSAGGRVLNLVDLSDVSMTFFLPTAQAGRVAIGADARIV 248 Query: 311 LDAYPDHPIPAKVSFVASHAQFTPKTVETQTERDKLMFRIRTRIDEERLLKHAESVRSGL 370 LDA P + IPAKVSFVA AQFTPKTVE++ ER KLMFR++ +I + L K+ + V++GL Sbjct: 249 LDAAPQYVIPAKVSFVADVAQFTPKTVESEEERQKLMFRVKAKIPQILLQKYIQQVKTGL 308 Query: 371 PGMAYVRWDAKLAWPKNL 388 PG+AYV+ WPKNL Sbjct: 309 PGVAYVKLAPDAGWPKNL 326 >yhiI [M] COG0845 Membrane-fusion protein Eco Escherichia coli K12 Length = 355 Score = 230 bits (587), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 146/338 (43%), Positives = 202/338 (59%), Gaps = 29/338 (8%) Query: 80 VLGGVVGTGYYVWKKAHPP-LPVGISLGNGRIEADEIDIATKFAGRVSELLVDIGDIVAP 138 V+G + W P +P G ++ NGRIEA E+DIA+K AGR+ +LV G V Sbjct: 12 VVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIEATEVDIASKIAGRIDTILVKEGKFVRE 71 Query: 139 GQVLARMDTRDIEQSLSKAEAQTRQALRAVEEAQANLAQQQT----TKTLAEQEMG---- 190 G+VLA+MDTR +++ +A AQ ++A AV AQA L Q+Q+ ++L Q Sbjct: 72 GEVLAKMDTRVLQEQRLEAIAQIKEAQSAVAAAQALLEQRQSETRAAQSLVNQRQAELDS 131 Query: 191 ------RTNSLLKNG-WTTQEIADQR-------------KQSLDAAIAGLNAAQARVLVA 230 R+ SL + G + Q++ D R K + A+ A + AA+ ++ A Sbjct: 132 VAKRHTRSRSLAQRGAISAQQLDDDRAAAESARAALESAKAQVSASKAAIEAARTNIIQA 191 Query: 231 QHAREAAQHDAGFYRVQIADNTLVAPKAGRIQYRLANIGEVLPGGGKVFTMLDLSYVYMD 290 Q EAAQ I D+ L AP+ GR+QYR+A GEVL GG+V M+DLS VYM Sbjct: 192 QTRVEAAQATERRIAADIDDSELKAPRDGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMT 251 Query: 291 IYLPTLEAGKVKVGADARIVLDAYPDHPIPAKVSFVASHAQFTPKTVETQTERDKLMFRI 350 +LPT +AG +K+G +AR++LDA PD IPA +SFVAS AQFTPKTVET ER KLMFR+ Sbjct: 252 FFLPTEQAGTLKLGGEARLILDAAPDLRIPATISFVASVAQFTPKTVETSDERLKLMFRV 311 Query: 351 RTRIDEERLLKHAESVRSGLPGMAYVRWDAKLAWPKNL 388 + RI E L +H E V++GLPG+A+VR + +L WP +L Sbjct: 312 KARIPPELLQQHLEYVKTGLPGVAWVRVNEELPWPDDL 349 >ECs4360 [M] COG0845 Membrane-fusion protein Ecs Escherichia coli O157:H7 Length = 355 Score = 229 bits (584), Expect = 8e-60, Method: Compositional matrix adjust. Identities = 142/318 (44%), Positives = 196/318 (61%), Gaps = 28/318 (8%) Query: 99 LPVGISLGNGRIEADEIDIATKFAGRVSELLVDIGDIVAPGQVLARMDTRDIEQSLSKAE 158 +P G ++ NGRIEA E+DIA+K AGR+ +LV G V G+VLA+MDTR +++ +A Sbjct: 32 VPEGFAVSNGRIEATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAI 91 Query: 159 AQTRQALRAVEEAQANLAQQQT----TKTLAEQEMG----------RTNSLLKNG-WTTQ 203 AQ ++A AV AQA L Q+Q+ ++L Q R+ SL + G + Q Sbjct: 92 AQIKEAQSAVAAAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQ 151 Query: 204 EIADQR-------------KQSLDAAIAGLNAAQARVLVAQHAREAAQHDAGFYRVQIAD 250 ++ D R K + A+ A + AA+ ++ AQ EAAQ I D Sbjct: 152 QLDDDRAAAESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDD 211 Query: 251 NTLVAPKAGRIQYRLANIGEVLPGGGKVFTMLDLSYVYMDIYLPTLEAGKVKVGADARIV 310 + L AP+ GR+QYR+A GEVL GG+V M+DLS VYM +LPT +AG +K+G +AR++ Sbjct: 212 SELKAPRDGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLI 271 Query: 311 LDAYPDHPIPAKVSFVASHAQFTPKTVETQTERDKLMFRIRTRIDEERLLKHAESVRSGL 370 LDA PD IPA +SFVAS AQFTPKTVET ER KLMFR++ RI E L +H E V++GL Sbjct: 272 LDAAPDLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGL 331 Query: 371 PGMAYVRWDAKLAWPKNL 388 PG+A+VR + +L WP +L Sbjct: 332 PGVAWVRVNEELPWPDDL 349