BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0115.0005.p (344 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Cgl2071 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 412 e-115 SMb21003 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene te... 318 1e-86 mlr4867 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 313 5e-85 AGc4197 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 310 2e-84 SP0267 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 293 5e-79 >Cgl2071 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Cgl Corynebacterium glutamicum Length = 348 Score = 412 bits (1060), Expect = e-115, Method: Compositional matrix adjust. Identities = 193/343 (56%), Positives = 263/343 (76%), Gaps = 5/343 (1%) Query: 4 SVEFGLDTFGDVTNDAALVPLPHAQVIRNVVDEAVLADQLGIDFIGLGEHHRADFAISTP 63 +V FGLDTFGD D P+ AQ +RN++DEA +AD++G+D IG+GEHHR ++++S P Sbjct: 3 NVSFGLDTFGDNAIDLQGNPVSPAQTLRNIIDEAKMADKVGVDIIGIGEHHREEYSVSAP 62 Query: 64 ETVLAAIAARTENIRLGSAVTVLSSDDPIRVFQRFATVDAVSNGRAEVILGRGSFTESFP 123 + V+ AI A TE +++ S+VTVLSSDDP+R+F+R++T++A+SNGRAE+ LGRGSF ESFP Sbjct: 63 DIVMTAILASTERLKVTSSVTVLSSDDPVRLFERYSTMNALSNGRAEITLGRGSFIESFP 122 Query: 124 LFGFDLRQYNELFEEKLDLFTELLKQEP----VTWQGKLRPPLKSQSVYPPVEHGRLKTW 179 LFGFDL+ Y +LF E+LDLF ++L+ + VTW G+ R L++Q +YPP E+G + W Sbjct: 123 LFGFDLQDYEQLFSERLDLFAKILEADSRGQGVTWHGETRSALENQMLYPPTENG-IHAW 181 Query: 180 IGVGGSPESVVRAAHYDLPLMLAIIGGDPKRFAPYVDLHQRAYQQFGREPKPIGVHSPGY 239 + VGGSPESVVRAA Y PLMLAIIGG P+RF PYVDL++RA +QFG+ KPIGVHSPG Sbjct: 182 VAVGGSPESVVRAAKYRFPLMLAIIGGAPERFRPYVDLYKRANEQFGQPQKPIGVHSPGL 241 Query: 240 VAETDAQAREELWPDYKVMRDRIGKERGWPPMGRDEFVSEAEHGSLYVGAPETVARKIAA 299 +A TD +ARE D+ ++ +IG ERGW P +F E +HGSLY+G+PETVA+KIA Sbjct: 242 IAATDEEARELALNDWLELQRKIGAERGWAPADAMQFEREIDHGSLYIGSPETVAKKIAK 301 Query: 300 TVKALGAARFQLKYSAGPLPHDKLMKSIELYGSKVVPMVRDML 342 T+ L RF LKY++G PH+ L+KSIELYG++V+P+V+D+L Sbjct: 302 TISVLDLDRFTLKYASGQTPHEYLLKSIELYGTEVIPLVKDIL 344 >SMb21003 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Sme Sinorhizobium meliloti Length = 347 Score = 318 bits (815), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 178/345 (51%), Positives = 232/345 (67%), Gaps = 9/345 (2%) Query: 5 VEFGLDTFGDVTNDAALVPLPHAQVIRNVVDEAVLADQLGIDFIGLGEHHRADFAISTPE 64 +E GL TF DV +AA + + ++++E LADQ+G+D GLGEHHR D+A S P Sbjct: 1 MELGLYTFADVDPNAADKGAEGRRRLADLLEEIELADQVGLDVFGLGEHHRPDYAASAPA 60 Query: 65 TVLAAIAARTENIRLGSAVTVLSSDDPIRVFQRFATVDAVSNGRAEVILGRGSFTESFPL 124 +LAA AART IRL SAVTVLSSDDP+RVFQ+FAT+D +SNGRAE++ GRGSF ESFPL Sbjct: 61 VILAAAAARTSRIRLTSAVTVLSSDDPVRVFQQFATLDLLSNGRAEIMAGRGSFIESFPL 120 Query: 125 FGFDLRQYNELFEEKLDLFTELLKQEPVTWQGKLRPPLKSQSVYPPVEHGRLKTWIGVGG 184 FG L Y +LF EKLDL L E VTW G LR P+ + VYP L WI VGG Sbjct: 121 FGQSLDDYEQLFSEKLDLLLALRDSERVTWSGALRSPINDRGVYPRPLQDPLPLWIAVGG 180 Query: 185 SPESVVRAAHYDLPLMLAIIGGDPKRFAPYVDLHQRAYQQFGREPK----PIGVHSPGYV 240 +P+SV RA +P+ LAIIGG+P RFAP L++ A ++ G++P I VH G++ Sbjct: 181 TPQSVARAGALGVPMALAIIGGEPARFAPLFQLYREAGRRSGQDPSKLKTSINVH--GFI 238 Query: 241 AETDAQAREELW-PDYKVMRDRIGKERGWPPMGRDEFVSE-AEHGSLYVGAPETVARKIA 298 A+T +A ++ + P +VM +RIG+ERGW P R F + GSL++G PETVARKI Sbjct: 239 ADTTQEAADQFYGPQAEVM-NRIGRERGWGPTNRAHFDQAISPTGSLFLGDPETVARKIV 297 Query: 299 ATVKALGAARFQLKYSAGPLPHDKLMKSIELYGSKVVPMVRDMLA 343 A K G RF L+ + GP+PHD+++ IELYG+KV P+VR+MLA Sbjct: 298 AHRKLFGNDRFLLQMAIGPMPHDRILHGIELYGTKVAPLVREMLA 342 >mlr4867 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Mlo Mesorhizobium loti Length = 353 Score = 313 bits (801), Expect = 5e-85, Method: Compositional matrix adjust. Identities = 173/345 (50%), Positives = 228/345 (66%), Gaps = 9/345 (2%) Query: 5 VEFGLDTFGDVTNDAALVPLPHAQVIRNVVDEAVLADQLGIDFIGLGEHHRADFAISTPE 64 +E GL TF DV+ + + +RN+++E LADQ+G+D GLGEHHR D+A S P Sbjct: 1 MELGLYTFADVSPQPGPGAIGPHERLRNLIEEVELADQVGLDVFGLGEHHRPDYAASAPV 60 Query: 65 TVLAAIAARTENIRLGSAVTVLSSDDPIRVFQRFATVDAVSNGRAEVILGRGSFTESFPL 124 LAA A R++ I+L SAVTVLSSDDP+RVFQ+FAT+D +S GRAE++ GRGSF ESFPL Sbjct: 61 VALAAAAERSKRIKLTSAVTVLSSDDPVRVFQQFATLDLLSGGRAEIMAGRGSFIESFPL 120 Query: 125 FGFDLRQYNELFEEKLDLFTELLKQEPVTWQGKLRPPLKSQSVYPPVEHGRLKTWIGVGG 184 FG++L Y+ELF EKLDL + VTW G LR P+ + VYP RL WI +GG Sbjct: 121 FGYNLEDYDELFAEKLDLLLAIRDSVKVTWSGNLRAPINDRGVYPRPFQDRLPVWIAIGG 180 Query: 185 SPESVVRAAHYDLPLMLAIIGGDPKRFAPYVDLHQRAYQQFGREP----KPIGVHSPGYV 240 +P+S RA LPL LAIIGG+P RFAP DL++ A ++ G +P I VH G++ Sbjct: 181 TPQSAARAGALGLPLALAIIGGEPARFAPLFDLYREAARRAGSDPTGLATSINVH--GFI 238 Query: 241 AETDAQAREELW-PDYKVMRDRIGKERGWPPMGRDEF-VSEAEHGSLYVGAPETVARKIA 298 AET QA ++ + P +VM +RIG+ERGW P R F S +G+L+VG+PE VA KI Sbjct: 239 AETTEQAADDFYGPQAEVM-NRIGRERGWGPTSRAHFDQSRGPNGALFVGSPEQVAEKIV 297 Query: 299 ATVKALGAARFQLKYSAGPLPHDKLMKSIELYGSKVVPMVRDMLA 343 A + RF L+ + G +PH K+MK+IELYG+KV P+VR A Sbjct: 298 AQHRIFNNDRFLLQMAIGTMPHAKIMKAIELYGTKVAPIVRKETA 342 >AGc4197 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 353 Score = 310 bits (795), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 172/345 (49%), Positives = 230/345 (66%), Gaps = 10/345 (2%) Query: 5 VEFGLDTFGDVTNDAALVPLPH-AQVIRNVVDEAVLADQLGIDFIGLGEHHRADFAISTP 63 +E GL TF DV + A P A+ +R +++E LADQ+G+D GLGEHHR D+ +S+P Sbjct: 1 MELGLYTFADVNPNPADGRGPEGARRLRELLEEIELADQVGLDVFGLGEHHRPDYVVSSP 60 Query: 64 ETVLAAIAARTENIRLGSAVTVLSSDDPIRVFQRFATVDAVSNGRAEVILGRGSFTESFP 123 TVLAA A +T+NIRL SAV+VLSSDDP+RVFQ+F+TVD +SNGRAE++ GRGSF ES+P Sbjct: 61 STVLAAAAVKTKNIRLTSAVSVLSSDDPVRVFQQFSTVDLLSNGRAEIMAGRGSFIESYP 120 Query: 124 LFGFDLRQYNELFEEKLDLFTELLKQEPVTWQGKLRPPLKSQSVYPPVEHGRLKTWIGVG 183 LFG+DL Y+ LF EKLDL L +QE VTW G P + + VYP RL WI VG Sbjct: 121 LFGYDLEDYDVLFAEKLDLLLALREQEVVTWSGTKHPAINGRGVYPRPLQERLPVWIAVG 180 Query: 184 GSPESVVRAAHYDLPLMLAIIGGDPKRFAPYVDLHQRAYQQFGREP----KPIGVHSPGY 239 G+P+SV RA LP+ LAIIGG+ +RFAP DL+ A ++ G+E I VH G+ Sbjct: 181 GTPQSVARAGAMGLPVALAIIGGEYRRFAPLFDLYHEAARRAGQEKTKLRTSINVH--GF 238 Query: 240 VAETDAQAREELW-PDYKVMRDRIGKERGWPPMGRDEF-VSEAEHGSLYVGAPETVARKI 297 +A+T +A ++ + P +VM +RIG+ERGW P R F + G+L++G PE VA KI Sbjct: 239 IADTTDKAADQFYGPQAEVM-NRIGRERGWGPTNRAHFDAARGPEGNLFLGEPELVAEKI 297 Query: 298 AATVKALGAARFQLKYSAGPLPHDKLMKSIELYGSKVVPMVRDML 342 RF L+ + G +PHD++M+ IELYG+KV P+VR L Sbjct: 298 IKAHGVFKNDRFLLQMAIGLMPHDQIMRGIELYGTKVAPLVRKEL 342 >SP0267 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Spn Streptococcus pneumoniae TIGR4 Length = 349 Score = 293 bits (749), Expect = 5e-79, Method: Compositional matrix adjust. Identities = 152/348 (43%), Positives = 226/348 (64%), Gaps = 13/348 (3%) Query: 5 VEFGLDTFGDVTN-DAALVPLPHAQVIRNVVDEAVLADQLGIDFIGLGEHHRADFAISTP 63 VE G+ TFG++T + HA+ IR +V E LAD++G+D G+GEHHRADFA+S P Sbjct: 2 VELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLDVYGIGEHHRADFAVSAP 61 Query: 64 ETVLAAIAARTENIRLGSAVTVLSSDDPIRVFQRFATVDAVSNGRAEVILGRGSFTESFP 123 E VLAA A T+ IRL SAV++LSS DPIR+FQ++AT+DA+SNGR+E++ GRGSFTESFP Sbjct: 62 EIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDALSNGRSEIMAGRGSFTESFP 121 Query: 124 LFGFDLRQYNELFEEKLDLFTELLKQEPVTWQGKLRPPLKSQSVYPPVEHGRLKTWIGVG 183 LFG+DL+ Y+ LF+EKLDL + ++ + WQG+L + + VYP +L WI G Sbjct: 122 LFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTIAGKEVYPRPVQDKLPLWIATG 181 Query: 184 GSPESVVRAAHYDLPLMLAIIGGDPKRFAPYVDLHQRAYQQFGREPK------PIGVHSP 237 G ES V+ A LP++ AIIGG+P+ F + +AY++ G E +G HS Sbjct: 182 GHVESTVKIAQAGLPIVYAIIGGNPRYFKKLI----QAYREIGSEAGHADKDLKVGAHSW 237 Query: 238 GYVAETDAQAREELWPDYKVMRDRIGKER-GWPPMGRDEFVSE-AEHGSLYVGAPETVAR 295 G++AE QA ++ + K + D I K+R W + ++++ + +G+++VG P+ VA Sbjct: 238 GWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQYLEQVGPNGAMFVGNPDQVAE 297 Query: 296 KIAATVKALGAARFQLKYSAGPLPHDKLMKSIELYGSKVVPMVRDMLA 343 K+ ++ LG RF L G +PHD+++++IEL+G++V P VR A Sbjct: 298 KLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGTQVAPKVRAYFA 345