BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0114.0008.p (253 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGpA200 [R] COG0491 Zn-dependent hydrolases, including glyoxylas... 132 7e-31 AGpT140 [R] COG0491 Zn-dependent hydrolases, including glyoxylas... 95 2e-19 mlr6805 [R] COG0491 Zn-dependent hydrolases, including glyoxylas... 81 2e-15 TVN1285 [R] COG0491 Zn-dependent hydrolases, including glyoxylas... 80 3e-15 DR0172 [R] COG0491 Zn-dependent hydrolases, including glyoxylase... 75 1e-13 >AGpA200 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 263 Score = 132 bits (332), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 84/261 (32%), Positives = 125/261 (47%), Gaps = 11/261 (4%) Query: 1 MKMHMLSGGRLQMSKRTYLPDAARGEMIELPVSCVLLRHTQGNVLFDTGCHPDVATDPEG 60 ++++ML G L+ + G E+PV L+ H G+ + D G +VATDP G Sbjct: 4 IRLYMLQSGTLKCKVHNIKMNQGNGADYEIPVPFFLITHPAGHTVIDGGNAIEVATDPRG 63 Query: 61 RLGTLAKYMRPIMPADDHVLTSLKAVGLGADDIDVVICSHLHTDHCGCNAFFKKATIFVH 120 G + P++ D + +KA+G D+ V+ SHLH DH G F AT V Sbjct: 64 HWGGICDVYWPVLDKDQGCVDQIKALGFDPADVKYVVQSHLHLDHTGAIGRFPNATHIVQ 123 Query: 121 ALEVEAANADNAF-DRGYIKADWDHP-LKMEIF----DKQVDVFGDGKLTLVPLPGHSKG 174 E E A + F GYI+ D+D P LK + D DV+GDG LT + PGH+ G Sbjct: 124 RSEYEYAFTPDWFAGGGYIRKDFDKPGLKWQFLNGAQDDYYDVYGDGTLTTIFTPGHAPG 183 Query: 175 TTGALVKLDNSGEFLLASDALSVRANLDQRTSP---RNSLDVDQFLKSLDEIARLEASGV 231 LV+L NS LL DA + +++ P +++D + ++ L A E Sbjct: 184 HQSFLVRLPNSKPLLLTIDAAYTLDHWEEKALPGFLASTVDTVRSVQKLRTYA--EKHDA 241 Query: 232 KIICGHDEAQWASLQKGPNAY 252 ++ GHD WA+ +K P Y Sbjct: 242 TVVTGHDPDAWANFKKAPEFY 262 >AGpT140 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 276 Score = 94.7 bits (234), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 74/241 (30%), Positives = 113/241 (46%), Gaps = 17/241 (7%) Query: 26 EMIELPVSCVLLRHTQGNVLFDTGCHPDVATDPEGRLGTLAKYMRPIMPADD-HVLTSLK 84 +I++PVS L++ T VL+DTGCHP+ GR ++ P + A + ++ L+ Sbjct: 37 RLIDIPVSAYLIQCTDATVLYDTGCHPE-CMGTNGRWPAQSQLNAPYIGASECNLPERLR 95 Query: 85 AVGLGADDIDVVICSHLHTDHCGCNAFFKKATIFVHALEVEAANADNAFDRG-----YIK 139 +GL DDI V+ SHLH DH GC +F K+ + H E E A A F G YI Sbjct: 96 QLGLSPDDISTVVLSHLHNDHAGCVEYFGKSRLIAH--EDEFATAVRYFATGDHSSPYIV 153 Query: 140 ADWD----HPLKMEIF--DKQVDVFGDGKLTLVPLPGHSKGTTGALVKLDNSGEFLLASD 193 D + P ++ D++ G L GH+ G G V+L+ FLL SD Sbjct: 154 KDIEAWLATPRNWDLVGRDERERELAPGVNLLNFGTGHASGMLGLAVRLEKQPGFLLVSD 213 Query: 194 ALSVRANLDQRTSPRNSL-DVDQFLKSLDEIARL-EASGVKIICGHDEAQWASLQKGPNA 251 A N L D + +++ I + E+ + ++ GHD Q+ASL K + Sbjct: 214 ACYTATNYGPPARRAGVLHDTIGYDRTVSHIRQYAESRSLTVLFGHDREQFASLIKSTDG 273 Query: 252 Y 252 + Sbjct: 274 F 274 >mlr6805 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Mlo Mesorhizobium loti Length = 268 Score = 81.3 bits (199), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 69/272 (25%), Positives = 108/272 (39%), Gaps = 30/272 (11%) Query: 2 KMHMLSGGRLQMSKRTYLPDAARGEMIELPVSCVLLRHTQGNVLFDTGCHPDVATDPEGR 61 K+++L GG L + + G + PV +L+ H +G L DTG D Sbjct: 5 KVYLLDGGSLVLDGYHVFWNRGPGGEVRFPVYSILIEHAEGRFLIDTGYDYD-------H 57 Query: 62 LGTLAKYMRPIMPADDHVLTSLKAVGLGADDIDVVICSHLHTDHCGCNAFFKKATIFVHA 121 + + + +PI + +L +GL DIDVV+ SH H DHCG N +F A H Sbjct: 58 VMKVLPFEKPIQEKHQTIPGALGLLGLEPRDIDVVVNSHFHFDHCGGNKYFPHAKKICHR 117 Query: 122 LEVEAANADNAFDR-GY-------------------IKADWDHPLKMEIFDKQVDVFGDG 161 EV A F+ GY ++ E D VD+ Sbjct: 118 SEVPQACNPQPFEHLGYSDLSFSAEAAEARGATAQLLEGTTRANSTFEGIDGDVDLARGV 177 Query: 162 KLTLVPLPGHSKGTTGALVKLDNSGEFLLASDALSVRANLDQRTSPRNSLDVDQFLKSLD 221 K L+ PGHS G LV+ L DA + +L+ +D + S+ Sbjct: 178 K--LISTPGHSIGHYSLLVEFPRRKPILFTIDAAYTQKSLETLCQAAFHIDPVAGVNSMR 235 Query: 222 EIARL-EASGVKIICGHDEAQWASLQKGPNAY 252 ++ +L E G +++ HD + + + G Y Sbjct: 236 KVKKLAEDHGAELMYSHDMDNFKTYRTGTQFY 267 >TVN1285 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Tvo Thermoplasma volcanium Length = 271 Score = 80.5 bits (197), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 73/260 (28%), Positives = 109/260 (41%), Gaps = 35/260 (13%) Query: 8 GGRLQMSKRTYLPDAARGEMIELPVSCVLLRHTQGNVLFDTGCHPDVATDPEGRLGTLAK 67 GG + S+R R + +E+P++ L+ H +LFDTG PD E G + Sbjct: 27 GGAMTNSERN-----PRRDWVEIPITGALIDHPDARILFDTGIAPDAMKTHE--RGLMEA 79 Query: 68 YMRPIMPADD--HVLTSLKAVGLGADDIDVVICSHLHTDHCGCNAFFKKA--TIFVHALE 123 + PI DD H+ LK +GL +DID V+ SHLH DH G A FK + I V E Sbjct: 80 F--PITKMDDQNHIENQLKLIGLKPEDIDFVVISHLHLDHIGQAAVFKDSHVPIIVQKKE 137 Query: 124 VEAA------NADNAFDRGYI----KADWDHPLKMEIFDKQVDVFGDGKLTLVPLPGHSK 173 +E+A A+D + A W PL +F+ V+ + GH+ Sbjct: 138 LESALSLIWQGKSGAYDYSDLLPLKGAAW-MPLDDPVFEIVDGVYAEFT------GGHTA 190 Query: 174 GTTGALVKLDNSGEFLLASDALSVRANLDQRTSP---RNSLDVDQFLKSLDEIARLEASG 230 G VK + + L D L + D N+ + + ++K + R +A Sbjct: 191 GHQVLTVKTEKGSMYTLTGDFLHLPKEYDVEAKGWLLSNATEWNSYIKKMK--IREKARK 248 Query: 231 VKIICGHDEAQWASLQKGPN 250 K+I HD W P Sbjct: 249 QKLIISHDPGIWDKYPHAPK 268 >DR0172 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Dra Deinococcus radiodurans Length = 271 Score = 75.5 bits (184), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 58/212 (27%), Positives = 87/212 (41%), Gaps = 25/212 (11%) Query: 1 MKMHMLSGGRLQMSKRTYLPDAA-----------RGEMIELPVSCVLLRHTQGNVLFDTG 49 +K+H S G++Q+ + P+ R LPV C L+ H G ++ DTG Sbjct: 4 LKIHGFSTGQIQVKSKFLRPEPQNLLGSLRSFLDRQYSAPLPVWCWLIEHPSGLIVVDTG 63 Query: 50 CHPDVATDPEGRLGTLAKYMRPIMPADDHVLTSLKAVGLGADDIDVVICSHLHTDHCGCN 109 ++ R T Y D + ++ G + V+I +HLH DH Sbjct: 64 LTCTARSNWFSRTQTRLHYT-----EHDRLSRQMQRAGFDPAQVRVLILTHLHIDHDRGM 118 Query: 110 AFFKKATIFVHALEVEAANADNAFDRGYIKADWDH--------PLKMEIFDKQVDVFGDG 161 F +A + V A E+ + A GY W PL F + DV GDG Sbjct: 119 GDFPEAHLLVSARELRDYRSPLARLMGYSAPTWKSVPHAAPFLPLAHGPFRESADVMGDG 178 Query: 162 KLTLVPLPGHSKGTTGALVKLDNSGEFLLASD 193 ++ L+P PGH+ G LV+ + LLA D Sbjct: 179 RVLLLPTPGHTPGHLSVLVR-QPGHDILLAGD 209