BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0109.0028.p (576 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value asnB [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) Eco... 682 0.0 STM0680 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) ... 681 0.0 ZasnB [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) Ec... 681 0.0 ECs0704 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) ... 681 0.0 VC0991 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) V... 677 0.0 >asnB [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) Eco Escherichia coli K12 Length = 554 Score = 682 bits (1759), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/553 (58%), Positives = 401/553 (72%), Gaps = 3/553 (0%) Query: 1 LCGFVGIFGFKESIETVRSRALEMAKTVRHRGPDWSGIYSGEGAVLAHERLSIVDVEHGA 60 +C G+F K +R +ALE+++ +RHRGPDWSGIY+ + A+LAHERLSIVDV GA Sbjct: 1 MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNAILAHERLSIVDVNAGA 60 Query: 61 QPLLDVTTGRALAVNGEIYNHVALREALKLPHAWKTKSDCEIILYLYDEYGPAVCNMLSG 120 QPL + LAVNGEIYNH ALR + ++T SDCE+IL LY E GP + L G Sbjct: 61 QPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEVILALYQEKGPEFLDDLQG 120 Query: 121 IFAFALFDERSGDFFVARDHIGIVPLYIGWGSDGTTYVASEMKALDGVCETIREFPPGHH 180 +FAFAL+D + + RDH+GI+PLY+G+ G YVASEMKAL VC TI+EFP G + Sbjct: 121 MFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVASEMKALVPVCRTIKEFPAGSY 180 Query: 181 YSGKAGKFVKWYDPQW--AHTLPTRKVSLQELRTSLEAAVKQQMMCDVPYGVLISGGLDS 238 + G+ +Y W + ELR +LE +VK +M DVPYGVL+SGGLDS Sbjct: 181 LWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALEDSVKSHLMSDVPYGVLLSGGLDS 240 Query: 239 SLIAALAARHRFKRIESGETEEAWWPRLHSFSVGLENSPDMKHARDVAAHIGTVHHEIVF 298 S+I+A+ ++ +R+E E EAWWP+LHSF+VGL SPD+K A++VA H+GTVHHEI F Sbjct: 241 SIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLPGSPDLKAAQEVANHLGTVHHEIHF 300 Query: 299 TVQEGLDALPDVIRHLETFDVTTIRAGTPMYLMARKIKSMGIKMVLSGEGADEVFGGYLY 358 TVQEGLDA+ DVI H+ET+DVTTIRA TPMYLM+RKIK+MGIKMVLSGEG+DEVFGGYLY Sbjct: 301 TVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLY 360 Query: 359 FHMAPSAEEFHQETVRKIFALSKYDCCRANKAMAAWGVEARVPFLDKEFLDYAMSINPAE 418 FH AP+A+E H+ETVRK+ AL YDC RANKAM+AWGVEARVPFLDK+FLD AM INP + Sbjct: 361 FHKAPNAKELHEETVRKLLALHMYDCARANKAMSAWGVEARVPFLDKKFLDVAMRINPQD 420 Query: 419 KMCPGEKIEKGILREAFTDLLPQAILWRQKEQFSDGVGYSWIESLKHQAENKVSDQMLAA 478 KMC K+EK ILRE F LP ++ WRQKEQFSDGVGYSWI++LK A +VSDQ L Sbjct: 421 KMCGNGKMEKHILRECFEAYLPASVAWRQKEQFSDGVGYSWIDTLKEVAAQQVSDQQLET 480 Query: 479 ASKRFPQQTPTSKEGYFYRTIFDSIFNNPTACLTVPVGPSIACSTPTAFLWSQQFASMND 538 A RFP TPTSKE Y YR IF+ +F P+A VP GPS+ACS+ A W + F M+D Sbjct: 481 ARFRFPYNTPTSKEAYLYREIFEELFPLPSAAECVPGGPSVACSSAKAIEWDEAFKKMDD 540 Query: 539 PSGRAVKGIHVQA 551 PSGRAV G+H A Sbjct: 541 PSGRAV-GVHQSA 552 >STM0680 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) Sty Salmonella typhimurium LT2 Length = 554 Score = 681 bits (1758), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/553 (58%), Positives = 402/553 (72%), Gaps = 3/553 (0%) Query: 1 LCGFVGIFGFKESIETVRSRALEMAKTVRHRGPDWSGIYSGEGAVLAHERLSIVDVEHGA 60 +C G+F K +R +ALE+++ +RHRGPDWSGIY+ + A+LAHERLSIVDV GA Sbjct: 1 MCSIFGVFDIKTDAAELRKKALELSRLMRHRGPDWSGIYACDNAILAHERLSIVDVNAGA 60 Query: 61 QPLLDVTTGRALAVNGEIYNHVALREALKLPHAWKTKSDCEIILYLYDEYGPAVCNMLSG 120 QPL + LAVNGEIYNH LR +A++T SDCE+IL LY E GP + L G Sbjct: 61 QPLYNARKTHVLAVNGEIYNHQTLRAEYGDRYAFQTGSDCEVILALYQEKGPDFLDDLQG 120 Query: 121 IFAFALFDERSGDFFVARDHIGIVPLYIGWGSDGTTYVASEMKALDGVCETIREFPPGHH 180 +FAFAL+D + + RDHIGI+PLY+G+ G YVASEMKAL VC TI+EFP G + Sbjct: 121 MFAFALYDSEKDAYLIGRDHIGIIPLYMGYDEFGNFYVASEMKALTPVCRTIKEFPAGSY 180 Query: 181 YSGKAGKFVKWYDPQWAH--TLPTRKVSLQELRTSLEAAVKQQMMCDVPYGVLISGGLDS 238 K G+ ++Y W + LR +LE +VK +M DVPYGVL+SGGLDS Sbjct: 181 LWSKDGEIRQYYQRDWFEFDAVKDNVTDKNALRQALEESVKSHLMSDVPYGVLLSGGLDS 240 Query: 239 SLIAALAARHRFKRIESGETEEAWWPRLHSFSVGLENSPDMKHARDVAAHIGTVHHEIVF 298 S+I+A+ + +R+E E EAWWP+LHSF+VGLE SPD+K A++VA H+GTVHHEI F Sbjct: 241 SVISAITKKFAARRVEDQERSEAWWPQLHSFAVGLEGSPDLKAAQEVANHLGTVHHEIHF 300 Query: 299 TVQEGLDALPDVIRHLETFDVTTIRAGTPMYLMARKIKSMGIKMVLSGEGADEVFGGYLY 358 TVQEGLDA+ DVI H+ET+DVTTIRA TPMYLM+RKIK+MGIKMVLSGEG+DEVFGGYLY Sbjct: 301 TVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLY 360 Query: 359 FHMAPSAEEFHQETVRKIFALSKYDCCRANKAMAAWGVEARVPFLDKEFLDYAMSINPAE 418 FH AP+A+E H+ETVRK+ AL YDC RANKAM+AWGVEARVPFLDK+FLD AM INP + Sbjct: 361 FHKAPNAKELHEETVRKLQALHMYDCARANKAMSAWGVEARVPFLDKKFLDVAMRINPQD 420 Query: 419 KMCPGEKIEKGILREAFTDLLPQAILWRQKEQFSDGVGYSWIESLKHQAENKVSDQMLAA 478 KMC K+EK +LRE F LP ++ WRQKEQFSDGVGYSWI++LK A ++SDQ L Sbjct: 421 KMCGNGKMEKHVLRECFESYLPASVAWRQKEQFSDGVGYSWIDTLKEVAAKQISDQQLET 480 Query: 479 ASKRFPQQTPTSKEGYFYRTIFDSIFNNPTACLTVPVGPSIACSTPTAFLWSQQFASMND 538 A RFP TP+SKE Y YR IF+ +F P+A VP GPS+ACS+ A W + F +M+D Sbjct: 481 ARFRFPYNTPSSKEAYLYREIFEELFPVPSAAECVPGGPSVACSSAKAIEWDEAFKTMDD 540 Query: 539 PSGRAVKGIHVQA 551 PSGRAV G+H A Sbjct: 541 PSGRAV-GVHQSA 552 >ZasnB [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) EcZ Escherichia coli O157:H7 EDL933 Length = 554 Score = 681 bits (1757), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/553 (58%), Positives = 401/553 (72%), Gaps = 3/553 (0%) Query: 1 LCGFVGIFGFKESIETVRSRALEMAKTVRHRGPDWSGIYSGEGAVLAHERLSIVDVEHGA 60 +C G+F K +R +ALE+++ +RHRGPDWSGIY+ + A+LAHERLSIVDV GA Sbjct: 1 MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKAILAHERLSIVDVNAGA 60 Query: 61 QPLLDVTTGRALAVNGEIYNHVALREALKLPHAWKTKSDCEIILYLYDEYGPAVCNMLSG 120 QPL + LAVNGEIYNH ALR + ++T SDCE+IL LY E GP + L G Sbjct: 61 QPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEVILALYQEKGPEFLDDLQG 120 Query: 121 IFAFALFDERSGDFFVARDHIGIVPLYIGWGSDGTTYVASEMKALDGVCETIREFPPGHH 180 +FAFAL+D + + RDH+GI+PLY+G+ G YVASEMKAL VC TI+EFP G + Sbjct: 121 MFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVASEMKALVPVCRTIKEFPAGSY 180 Query: 181 YSGKAGKFVKWYDPQWAH--TLPTRKVSLQELRTSLEAAVKQQMMCDVPYGVLISGGLDS 238 + G+ +Y W + ELR +LE +VK +M DVPYGVL+SGGLDS Sbjct: 181 LWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALEDSVKSHLMSDVPYGVLLSGGLDS 240 Query: 239 SLIAALAARHRFKRIESGETEEAWWPRLHSFSVGLENSPDMKHARDVAAHIGTVHHEIVF 298 S+I+A+ ++ +R+E E EAWWP+LHSF+VGL SPD+K A++VA H+GTVHHEI F Sbjct: 241 SIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLPGSPDLKAAQEVANHLGTVHHEIHF 300 Query: 299 TVQEGLDALPDVIRHLETFDVTTIRAGTPMYLMARKIKSMGIKMVLSGEGADEVFGGYLY 358 TVQEGLDA+ DVI H+ET+DVTTIRA TPMYLM+RKIK+MGIKMVLSGEG+DEVFGGYLY Sbjct: 301 TVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLY 360 Query: 359 FHMAPSAEEFHQETVRKIFALSKYDCCRANKAMAAWGVEARVPFLDKEFLDYAMSINPAE 418 FH AP+A+E H+ETVRK+ AL YDC RANKAM+AWGVEARVPFLDK+FLD AM INP + Sbjct: 361 FHKAPNAKELHEETVRKLLALHMYDCARANKAMSAWGVEARVPFLDKKFLDVAMRINPQD 420 Query: 419 KMCPGEKIEKGILREAFTDLLPQAILWRQKEQFSDGVGYSWIESLKHQAENKVSDQMLAA 478 KMC K+EK ILRE F LP ++ WRQKEQFSDGVGYSWI++LK A +VSDQ L Sbjct: 421 KMCGNGKMEKHILRECFESYLPASVAWRQKEQFSDGVGYSWIDTLKEVAAQQVSDQQLET 480 Query: 479 ASKRFPQQTPTSKEGYFYRTIFDSIFNNPTACLTVPVGPSIACSTPTAFLWSQQFASMND 538 A RFP TPTSKE Y YR IF+ +F P+A VP GPS+ACS+ A W + F M+D Sbjct: 481 ARFRFPYNTPTSKEAYLYREIFEELFPLPSAAECVPGGPSVACSSAKAIEWDEAFKKMDD 540 Query: 539 PSGRAVKGIHVQA 551 PSGRAV G+H A Sbjct: 541 PSGRAV-GVHQSA 552 >ECs0704 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) Ecs Escherichia coli O157:H7 Length = 554 Score = 681 bits (1757), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/553 (58%), Positives = 401/553 (72%), Gaps = 3/553 (0%) Query: 1 LCGFVGIFGFKESIETVRSRALEMAKTVRHRGPDWSGIYSGEGAVLAHERLSIVDVEHGA 60 +C G+F K +R +ALE+++ +RHRGPDWSGIY+ + A+LAHERLSIVDV GA Sbjct: 1 MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKAILAHERLSIVDVNAGA 60 Query: 61 QPLLDVTTGRALAVNGEIYNHVALREALKLPHAWKTKSDCEIILYLYDEYGPAVCNMLSG 120 QPL + LAVNGEIYNH ALR + ++T SDCE+IL LY E GP + L G Sbjct: 61 QPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEVILALYQEKGPEFLDDLQG 120 Query: 121 IFAFALFDERSGDFFVARDHIGIVPLYIGWGSDGTTYVASEMKALDGVCETIREFPPGHH 180 +FAFAL+D + + RDH+GI+PLY+G+ G YVASEMKAL VC TI+EFP G + Sbjct: 121 MFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVASEMKALVPVCRTIKEFPAGSY 180 Query: 181 YSGKAGKFVKWYDPQWAH--TLPTRKVSLQELRTSLEAAVKQQMMCDVPYGVLISGGLDS 238 + G+ +Y W + ELR +LE +VK +M DVPYGVL+SGGLDS Sbjct: 181 LWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALEDSVKSHLMSDVPYGVLLSGGLDS 240 Query: 239 SLIAALAARHRFKRIESGETEEAWWPRLHSFSVGLENSPDMKHARDVAAHIGTVHHEIVF 298 S+I+A+ ++ +R+E E EAWWP+LHSF+VGL SPD+K A++VA H+GTVHHEI F Sbjct: 241 SIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLPGSPDLKAAQEVANHLGTVHHEIHF 300 Query: 299 TVQEGLDALPDVIRHLETFDVTTIRAGTPMYLMARKIKSMGIKMVLSGEGADEVFGGYLY 358 TVQEGLDA+ DVI H+ET+DVTTIRA TPMYLM+RKIK+MGIKMVLSGEG+DEVFGGYLY Sbjct: 301 TVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLY 360 Query: 359 FHMAPSAEEFHQETVRKIFALSKYDCCRANKAMAAWGVEARVPFLDKEFLDYAMSINPAE 418 FH AP+A+E H+ETVRK+ AL YDC RANKAM+AWGVEARVPFLDK+FLD AM INP + Sbjct: 361 FHKAPNAKELHEETVRKLLALHMYDCARANKAMSAWGVEARVPFLDKKFLDVAMRINPQD 420 Query: 419 KMCPGEKIEKGILREAFTDLLPQAILWRQKEQFSDGVGYSWIESLKHQAENKVSDQMLAA 478 KMC K+EK ILRE F LP ++ WRQKEQFSDGVGYSWI++LK A +VSDQ L Sbjct: 421 KMCGNGKMEKHILRECFESYLPASVAWRQKEQFSDGVGYSWIDTLKEVAAQQVSDQQLET 480 Query: 479 ASKRFPQQTPTSKEGYFYRTIFDSIFNNPTACLTVPVGPSIACSTPTAFLWSQQFASMND 538 A RFP TPTSKE Y YR IF+ +F P+A VP GPS+ACS+ A W + F M+D Sbjct: 481 ARFRFPYNTPTSKEAYLYREIFEELFPLPSAAECVPGGPSVACSSAKAIEWDEAFKKMDD 540 Query: 539 PSGRAVKGIHVQA 551 PSGRAV G+H A Sbjct: 541 PSGRAV-GVHQSA 552 >VC0991 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) Vch Vibrio cholerae Length = 554 Score = 677 bits (1748), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/553 (58%), Positives = 402/553 (72%), Gaps = 2/553 (0%) Query: 1 LCGFVGIFGFKESIETVRSRALEMAKTVRHRGPDWSGIYSGEGAVLAHERLSIVDVEHGA 60 +C GI K T+R ALEM+K +RHRGPDWSGIY+ + A+LAHERL+IV + GA Sbjct: 1 MCSIFGILDIKTDAATLRPIALEMSKKLRHRGPDWSGIYASDKAILAHERLAIVGLNSGA 60 Query: 61 QPLLDVTTGRALAVNGEIYNHVALREALKLPHAWKTKSDCEIILYLYDEYGPAVCNMLSG 120 QPL LAVNGEIYNH +RE K + ++T SDCE+IL LY + G + L+G Sbjct: 61 QPLYSPDRKLILAVNGEIYNHKEIRERYKGQYEFQTDSDCEVILALYRDKGADLLEELNG 120 Query: 121 IFAFALFDERSGDFFVARDHIGIVPLYIGWGSDGTTYVASEMKALDGVCETIREFPPGHH 180 IFAF L+DE ++ + RDHIGI+PLY G G YVASEMKAL VC+T+ EFPPG + Sbjct: 121 IFAFVLYDEEKDEYLIGRDHIGIIPLYQGHDEHGNFYVASEMKALVPVCKTLSEFPPGSY 180 Query: 181 YSGKAGKFVKWYDPQWA--HTLPTRKVSLQELRTSLEAAVKQQMMCDVPYGVLISGGLDS 238 S K ++Y W + S +EL +LEAAVK+Q+M DVPYGVL+SGGLDS Sbjct: 181 LSSKDQAVTRYYVRDWNSFDEVKDNVSSKEELTQALEAAVKRQLMTDVPYGVLLSGGLDS 240 Query: 239 SLIAALAARHRFKRIESGETEEAWWPRLHSFSVGLENSPDMKHARDVAAHIGTVHHEIVF 298 S+ +A+A R RIE E AWWP+LHSF++GLE +PD+K AR+VA IGTVHHE+ + Sbjct: 241 SITSAIAKRFAAMRIEDDEKSAAWWPQLHSFAIGLEGAPDLKAAREVAEKIGTVHHEMTY 300 Query: 299 TVQEGLDALPDVIRHLETFDVTTIRAGTPMYLMARKIKSMGIKMVLSGEGADEVFGGYLY 358 T+QEGLDA+ DVI H+ET+DVTTIRA TPM+LM RKIK+MGIKMVLSGEGADE+FGGYLY Sbjct: 301 TIQEGLDAIRDVIYHIETYDVTTIRASTPMFLMGRKIKAMGIKMVLSGEGADEIFGGYLY 360 Query: 359 FHMAPSAEEFHQETVRKIFALSKYDCCRANKAMAAWGVEARVPFLDKEFLDYAMSINPAE 418 FH AP+A+EFH+ETVRK+ AL+ +DC RANK++AAWGVE RVPFLDKEF+D AM +NPA+ Sbjct: 361 FHKAPNAKEFHEETVRKLLALNLFDCARANKSLAAWGVEGRVPFLDKEFIDVAMRLNPAD 420 Query: 419 KMCPGEKIEKGILREAFTDLLPQAILWRQKEQFSDGVGYSWIESLKHQAENKVSDQMLAA 478 KMC K+EK ILRE F LP++I WRQKEQFSDGVGY WI++LK AE K+SDQ + Sbjct: 421 KMCGNGKMEKHILRECFEHYLPESIAWRQKEQFSDGVGYGWIDTLKATAEAKISDQQMET 480 Query: 479 ASKRFPQQTPTSKEGYFYRTIFDSIFNNPTACLTVPVGPSIACSTPTAFLWSQQFASMND 538 A RFP TPT+KEGY YR IF+ +F +A VP GPS+ACS+ A W + F + D Sbjct: 481 AKFRFPYNTPTTKEGYVYREIFEELFPLESAARCVPGGPSVACSSAKAIEWDESFKNCID 540 Query: 539 PSGRAVKGIHVQA 551 PSGRAVK +H QA Sbjct: 541 PSGRAVKAVHKQA 553