BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0104.0110.p (370 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr9222 [R] COG2159 Predicted metal-dependent hydrolase of the T... 339 5e-93 mlr9221 [R] COG2159 Predicted metal-dependent hydrolase of the T... 244 2e-64 SMa1734 [R] COG2159 Predicted metal-dependent hydrolase of the T... 195 1e-49 slr0619 [R] COG2159 Predicted metal-dependent hydrolase of the T... 90 8e-18 AGc4595 [R] COG2159 Predicted metal-dependent hydrolase of the T... 64 5e-10 >mlr9222 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Mlo Mesorhizobium loti Length = 371 Score = 339 bits (870), Expect = 5e-93, Method: Compositional matrix adjust. Identities = 175/367 (47%), Positives = 240/367 (65%), Gaps = 4/367 (1%) Query: 5 IDRPMLDQEKTAA--SRLRIIDCDIHPSIHAHSDLNEFLPKRWQQHLKEYGSHLRTPYIG 62 +D +L+Q+ A S IDCDIH + ++ +DL+EFLP+ W++H + GS R Sbjct: 1 MDTNLLNQKPKVAAPSHTGFIDCDIHATWNSLADLDEFLPQYWREHRETIGSRWRQGLAK 60 Query: 63 TTPYPRSSPLIA-RRDAWPPTGGVPGSDLDFMRKQHLDPFDVEFGILQVLDLFIFSQQNL 121 T YPR S R+DAWP G VPG+DL M+KQ LD F+VE+GI+ V + + S++N+ Sbjct: 61 TMNYPRMSKGGGVRQDAWPSNGCVPGADLPMMQKQLLDLFNVEYGIM-VQPMELSSERNV 119 Query: 122 EFGAAIQRAINDWQLAFWAHRDPRLKASILVGQDGSDLGLAEIERCAKSGEYIQINVSPR 181 EFGAA+ A N+WQLA W +PRLK ++ + + +AEIER A ++Q+ R Sbjct: 120 EFGAALATAANEWQLAHWCDPEPRLKGAVQINVEDEKAAIAEIERRAGDRRFVQVQFPQR 179 Query: 182 ANEPLGRRRYWPIYERAQELDLPLGIHVGGYGGHAPTGGGWPSYYCEEHQSNAHTVASNL 241 EP+GRRRYWPI E P+ IH+GG GGH T GGWPSYY EEH + + + + Sbjct: 180 GVEPIGRRRYWPIMEACAANGFPIAIHIGGIGGHPSTAGGWPSYYHEEHPAYVQAMQNTV 239 Query: 242 TSLVLEGVPERFPKLKIVFIEGGFGWIPATMWRMDQHFERFRSEVPHLKRRPSEYVKQHF 301 SLV EGV P LK++ +E GFGW+P+ WR+D ++R R+EVP L+ +PSEYV++ Sbjct: 240 ISLVCEGVLSHIPNLKVILLESGFGWLPSLCWRLDTAWKRLRAEVPLLREKPSEYVRERI 299 Query: 302 WFTTQPIDEPDEARHLRQLIEWVGIDRLLFSSDYPHWDYDDARYAFKTPLTDAERRKIFN 361 W TTQP+DEP+ + L IEW+G DRL+FS+DYPHWD DD RYAFK L +A +RKIF+ Sbjct: 300 WLTTQPVDEPERPQDLLSTIEWIGEDRLMFSTDYPHWDQDDPRYAFKAALPEAWKRKIFH 359 Query: 362 SNARAVY 368 NAR +Y Sbjct: 360 DNARTLY 366 >mlr9221 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Mlo Mesorhizobium loti Length = 357 Score = 244 bits (624), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 141/356 (39%), Positives = 199/356 (55%), Gaps = 10/356 (2%) Query: 16 AASRLRIIDCDIHPSIHAHSDLNEFLPKRWQQHLKEYGSHLRTPYIGTTPYPRSSPLIAR 75 +S R IDCD+H ++ L +L W++H+ G L + + YP S PL R Sbjct: 5 VSSPARPIDCDVHVALPDTRALLPYLDDYWREHVIRRG--LDREDLELSSYPPSLPLSCR 62 Query: 76 RDAWPPTGGVPGSDLDFMRKQHLDPFDVEFGILQVL--DLFIFSQQNLEFGAAIQRAIND 133 D W P G+PGS + +R Q LDPF F I VL +FS+ + AA+ RAIN+ Sbjct: 63 PD-WRPQQGLPGSQFEQLRDQVLDPFQSRFAICNVLHGSQIMFSE---DLSAALCRAINN 118 Query: 134 WQLAFWAHRDPRLKASILVGQDGSDLGLAEIERCAKSGEYIQINVSPRANEPLGRRRYWP 193 W A W RDPRL+ASI+V +L +AEIE A ++Q+ + PLGRR+ WP Sbjct: 119 WISAEWLDRDPRLRASIVVPVHSPELAVAEIEHRAADLRFVQVLMLAMGEVPLGRRQNWP 178 Query: 194 IYERAQELDLPLGIHVGGYGGHAPTGGGWPSYYCEEHQSNAHTVASNLTSLVLEGVPERF 253 IY A+ DLP+GIH G + P+ GW S+Y +++ S A + L SLV+EGV +F Sbjct: 179 IYAAAERHDLPIGIHAGSSLRNPPSPLGWSSHYIDDYVSQAPGFSGALNSLVVEGVFNKF 238 Query: 254 PKLKIVFIEGGFGWIPATMWRMDQHFERFRSEVPHLKRRPSEYVKQHFWFTTQPIDEPDE 313 P+LK+V +E G W+PA +WR ++ + RSE P L R PSE +++H T QP D P Sbjct: 239 PRLKVVLLESGVTWLPAHLWRANKQWRGVRSETPWLTRPPSEIIREHVRLTVQPFDAPPT 298 Query: 314 ARHLRQLIEWVGIDR-LLFSSDYPHWDYDDARYAFKTPLTDAERRKIFNSNARAVY 368 ++ +I+ +G D LLFS+DYPHW +D A L+ K+ NA A Y Sbjct: 299 DGQVQTIIDQIGSDEMLLFSTDYPHWHFDGTD-AVPQGLSHESLSKMLADNALATY 353 >SMa1734 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Sme Sinorhizobium meliloti Length = 366 Score = 195 bits (496), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 104/282 (36%), Positives = 157/282 (55%), Gaps = 7/282 (2%) Query: 92 FMRKQHLDPFDVEFGILQVLDLFIFSQ-QNLEFGAAIQRAINDWQLAFWAHRDPRLKASI 150 M+++HLD ++++ IL + S N + A+ RA ND+ + +W +D R SI Sbjct: 77 IMKEKHLDKYNIDVAILTGDEPIEASTLANAHYANALCRAYNDYMIDYWLPKDSRFWGSI 136 Query: 151 LVGQDGSDLGLAEIERCAKSGEYIQINVSPRANEPLGRRRYWPIYERAQELDLPLGIHVG 210 +V L EI R +Q+ VS A P G Y PIYE E+ LP +H+G Sbjct: 137 IVAPQDPKLAAEEIRRLGSHPRIVQVLVSHGAQRPYGDPFYHPIYEACAEMGLPFAMHLG 196 Query: 211 GYGG--HAPTGGGWPSYYCEEHQSNAHTVASNLTSLVLEGVPERFPKLKIVFIEGGFGWI 268 G GG P G G +++ E H + +++ SL+ +GV E++P LK+V IE G W+ Sbjct: 197 GQGGVNSTPIGAGPSTFFWETHAILPQSAMTHMASLIAQGVFEKWPSLKVVIIECGVAWV 256 Query: 269 PATMWRMDQHFERFRSEVPHLKRRPSEYVKQHFWFTTQPIDEPDEARHLRQLIEWV-GID 327 P+ +WR+D +++ R E P LKR PSEY K + +TQP+++P+ +HL +E + G + Sbjct: 257 PSVLWRLDANYKALRKETPWLKRLPSEYFKTNIRMSTQPLEQPENVQHLWATLEAMDGEN 316 Query: 328 RLLFSSDYPHWDYDDARYAFKTPLTDAERRKIFNSNARAVYK 369 LLF+SDYPHWDYDD K + A R K+ NA VY+ Sbjct: 317 TLLFASDYPHWDYDDVT---KLHIPPAWREKVLGLNALDVYR 355 >slr0619 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Syn Synechocystis Length = 348 Score = 90.1 bits (222), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 79/297 (26%), Positives = 131/297 (44%), Gaps = 32/297 (10%) Query: 89 DLDFMRKQHLDPFDVEFGILQVLD--LFIFSQQNLEFGAAIQRAINDWQLAFWAHRDP-R 145 DL R H+D ++ ++ + + +F E + R ND QL+ P R Sbjct: 57 DLGAGRIAHMDATGIDIQVISITSPGVQVFEP---ELATTLARQAND-QLSEAIKNHPDR 112 Query: 146 LKASILVGQDGSDLGLAEIERCAKSGEYIQINV-SPRANEPLGRRRYWPIYERAQELDLP 204 V EIER + + + S E L ++YW I+ A+ P Sbjct: 113 FAGLAAVAPQSPQTAALEIERAIQKLGLCGVLINSHTGGEYLDDQKYWEIFAAAEANSAP 172 Query: 205 LGIH--------VGGYGGHAPTGGGWPSYYCEEHQSNAHTVASNLTSLVLEGVPERFPKL 256 + +H + + + GW + E A++ L++ GV + FPKL Sbjct: 173 IYLHPRTPSPEMISPFLDYGLYFAGW-GFTVE--------TATHGLRLIMGGVFDHFPKL 223 Query: 257 KIVFIEGGFGWIPATMWRMDQHF--ERFRSEVPHLKRRPSEYVKQHFWFTTQPIDEPDEA 314 IV G G +P + R+D + + VP + R PSEY K HF TT + P Sbjct: 224 TIVLGHMGEG-LPYWLQRLDNRYSLQVEIGAVPKMPRLPSEYFKDHFLITTSGVCSPPAL 282 Query: 315 RHLRQLIEWVGIDRLLFSSDYPHWDYDDA-RYAFKTPLTDAERRKIFNSNARAVYKF 370 RH ++E +G D +LF++DYP+ ++A + P++D ERRKI+ +NA +++ Sbjct: 283 RH---VLEVLGSDAVLFAADYPYESVEEAVSFLDNAPISDQERRKIYQTNAEKLFRL 336 >AGc4595 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 327 Score = 64.3 bits (155), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 77/300 (25%), Positives = 125/300 (41%), Gaps = 31/300 (10%) Query: 89 DLDFMRKQHLDPFDVEFGILQVLDLFIFSQQNLEFGAAIQRAINDWQLAFWAHRDPRLKA 148 D+ R + +D +E IL + + + + I R ND A R R A Sbjct: 38 DIQDTRLKLMDAHGIETMILSLNAPAVQAIPDKTKAIEIARRANDALAEECAKRPDRFLA 97 Query: 149 SILVGQDGSDLGLAEIERCAKSGEYIQINVSPRANEPLGRR-------RYWPIYERAQEL 201 + D E+ RC ++ V+ + E G+ +Y P + ++L Sbjct: 98 FAALPLQDPDAATQELRRCVNDLGFVGALVNGFSQEGDGQTPLYYDLPQYRPFWGEVEKL 157 Query: 202 DLPLGIHVGG--------YGGHAPTGGGWPSYYCEEHQSNAHTVASNLTSLVLEGVPERF 253 D+P +H Y GH G P++ + A A + L+ G+ + Sbjct: 158 DVPFYLHPRNPLPQDSRIYDGHPWLLG--PTW------AFAQETAVHALRLMASGLFDEH 209 Query: 254 PKLKIVFIEGGFGWIPATMWRMDQHFERFRSEVPHL--KRRPSEYVKQHFWFTTQPIDEP 311 P+L I+ G G +P MWR+D H + P KRR +Y ++F TT Sbjct: 210 PRLDIILGHMGEG-LPYMMWRID-HRNAWVKLPPRYPAKRRFVDYFNENFHITTS---GN 264 Query: 312 DEARHLRQLIEWVGIDRLLFSSDYPHWDYDDARYAF-KTPLTDAERRKIFNSNARAVYKF 370 + L I +G DR+LFS+D+P + D A F T + +A+R KI +NAR ++K Sbjct: 265 FRTQTLIDAILEIGADRILFSTDWPFENIDHASDWFHATSIAEADRVKIGRTNARRLFKL 324