BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0104.0109.p (387 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr9221 [R] COG2159 Predicted metal-dependent hydrolase of the T... 397 e-110 mlr9222 [R] COG2159 Predicted metal-dependent hydrolase of the T... 208 1e-53 SMa1734 [R] COG2159 Predicted metal-dependent hydrolase of the T... 162 8e-40 slr0619 [R] COG2159 Predicted metal-dependent hydrolase of the T... 81 3e-15 AGc4595 [R] COG2159 Predicted metal-dependent hydrolase of the T... 73 1e-12 >mlr9221 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Mlo Mesorhizobium loti Length = 357 Score = 397 bits (1019), Expect = e-110, Method: Compositional matrix adjust. Identities = 193/348 (55%), Positives = 245/348 (70%), Gaps = 4/348 (1%) Query: 39 IDCDIHPAVGGTRTTLLPYLSDHWKEQVVSRAID--GLDLNSYPPSMPLSGRADWRPADG 96 IDCD+H A+ TR LLPYL D+W+E V+ R +D L+L+SYPPS+PLS R DWRP G Sbjct: 12 IDCDVHVALPDTRA-LLPYLDDYWREHVIRRGLDREDLELSSYPPSLPLSCRPDWRPQQG 70 Query: 97 SKPGSDLAMVQRGAFDQLGASHAICNVVYGAQAVFDSYMAADFCKAINDWIAAEWLARDK 156 PGS ++ D + AICNV++G+Q +F ++A C+AIN+WI+AEWL RD Sbjct: 71 -LPGSQFEQLRDQVLDPFQSRFAICNVLHGSQIMFSEDLSAALCRAINNWISAEWLDRDP 129 Query: 157 RLRASIVVPIQAPDLAVEEIERRAGDNRFVSVLVPSQGETLLGRRHYWPIYQAAEKYKLP 216 RLRASIVVP+ +P+LAV EIE RA D RFV VL+ + GE LGRR WPIY AAE++ LP Sbjct: 130 RLRASIVVPVHSPELAVAEIEHRAADLRFVQVLMLAMGEVPLGRRQNWPIYAAAERHDLP 189 Query: 217 IAIHAGSAYRGAPSSIGWPSYRYEYYLAEAQAFQAQTLSLIYEGVFGKYPGLTVVLMESG 276 I IHAGS+ R PS +GW S+ + Y+++A F SL+ EGVF K+P L VVL+ESG Sbjct: 190 IGIHAGSSLRNPPSPLGWSSHYIDDYVSQAPGFSGALNSLVVEGVFNKFPRLKVVLLESG 249 Query: 277 VSWLPAFMWRANKTWRGVRVEVPWVEREPAAIIRDHFRVTMQPFDAPLDPTSVADIIDQI 336 V+WLPA +WRANK WRGVR E PW+ R P+ IIR+H R+T+QPFDAP V IIDQI Sbjct: 250 VTWLPAHLWRANKQWRGVRSETPWLTRPPSEIIREHVRLTVQPFDAPPTDGQVQTIIDQI 309 Query: 337 GSDKMFLFASDYPHWQFDGDDPIPPHLPRSLVQRMCADNPLETFPRLK 384 GSD+M LF++DYPHW FDG D +P L + +M ADN L T+PRL Sbjct: 310 GSDEMLLFSTDYPHWHFDGTDAVPQGLSHESLSKMLADNALATYPRLN 357 >mlr9222 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Mlo Mesorhizobium loti Length = 371 Score = 208 bits (530), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 125/345 (36%), Positives = 179/345 (51%), Gaps = 12/345 (3%) Query: 39 IDCDIHPAVGGTRTTLLPYLSDHWKEQ---VVSRAIDGLDLNSYPPSMPLSG--RADWRP 93 IDCDIH A + L +L +W+E + SR GL P M G R D P Sbjct: 21 IDCDIH-ATWNSLADLDEFLPQYWREHRETIGSRWRQGLAKTMNYPRMSKGGGVRQDAWP 79 Query: 94 ADGSKPGSDLAMVQRGAFDQLGASHAICNVVYGAQAVFDSYMAADFCKAINDWIAAEWLA 153 ++G PG+DL M+Q+ D + I + + A A N+W A W Sbjct: 80 SNGCVPGADLPMMQKQLLDLFNVEYGIMVQPMELSSERNVEFGAALATAANEWQLAHWCD 139 Query: 154 RDKRLRASIVVPIQAPDLAVEEIERRAGDNRFVSVLVPSQGETLLGRRHYWPIYQAAEKY 213 + RL+ ++ + ++ A+ EIERRAGD RFV V P +G +GRR YWPI +A Sbjct: 140 PEPRLKGAVQINVEDEKAAIAEIERRAGDRRFVQVQFPQRGVEPIGRRRYWPIMEACAAN 199 Query: 214 KLPIAIHAGSAYRGAPSSIGWPSYRYEYYLAEAQAFQAQTLSLIYEGVFGKYPGLTVVLM 273 PIAIH G ++ GWPSY +E + A QA Q +SL+ EGV P L V+L+ Sbjct: 200 GFPIAIHIGGIGGHPSTAGGWPSYYHEEHPAYVQAMQNTVISLVCEGVLSHIPNLKVILL 259 Query: 274 ESGVSWLPAFMWRANKTWRGVRVEVPWVEREPAAIIRDHFRVTMQPFDAPLDPTSVADII 333 ESG WLP+ WR + W+ +R EVP + +P+ +R+ +T QP D P P + I Sbjct: 260 ESGFGWLPSLCWRLDTAWKRLRAEVPLLREKPSEYVRERIWLTTQPVDEPERPQDLLSTI 319 Query: 334 DQIGSDKMFLFASDYPHWQFDGDDP---IPPHLPRSLVQRMCADN 375 + IG D++ +F++DYPHW D DDP LP + +++ DN Sbjct: 320 EWIGEDRL-MFSTDYPHW--DQDDPRYAFKAALPEAWKRKIFHDN 361 >SMa1734 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Sme Sinorhizobium meliloti Length = 366 Score = 162 bits (411), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 86/259 (33%), Positives = 138/259 (53%), Gaps = 4/259 (1%) Query: 127 AQAVFDSYMAADFCKAINDWIAAEWLARDKRLRASIVVPIQAPDLAVEEIERRAGDNRFV 186 A + +++ A C+A ND++ WL +D R SI+V Q P LA EEI R R V Sbjct: 101 ASTLANAHYANALCRAYNDYMIDYWLPKDSRFWGSIIVAPQDPKLAAEEIRRLGSHPRIV 160 Query: 187 SVLVPSQGETLLGRRHYWPIYQAAEKYKLPIAIHAGS--AYRGAPSSIGWPSYRYEYYLA 244 VLV + G Y PIY+A + LP A+H G P G ++ +E + Sbjct: 161 QVLVSHGAQRPYGDPFYHPIYEACAEMGLPFAMHLGGQGGVNSTPIGAGPSTFFWETHAI 220 Query: 245 EAQAFQAQTLSLIYEGVFGKYPGLTVVLMESGVSWLPAFMWRANKTWRGVRVEVPWVERE 304 Q+ SLI +GVF K+P L VV++E GV+W+P+ +WR + ++ +R E PW++R Sbjct: 221 LPQSAMTHMASLIAQGVFEKWPSLKVVIIECGVAWVPSVLWRLDANYKALRKETPWLKRL 280 Query: 305 PAAIIRDHFRVTMQPFDAPLDPTSVADIIDQIGSDKMFLFASDYPHWQFDGDDPIPPHLP 364 P+ + + R++ QP + P + + ++ + + LFASDYPHW D DD H+P Sbjct: 281 PSEYFKTNIRMSTQPLEQPENVQHLWATLEAMDGENTLLFASDYPHW--DYDDVTKLHIP 338 Query: 365 RSLVQRMCADNPLETFPRL 383 + +++ N L+ + R+ Sbjct: 339 PAWREKVLGLNALDVYRRI 357 >slr0619 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Syn Synechocystis Length = 348 Score = 81.3 bits (199), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 75/257 (29%), Positives = 113/257 (43%), Gaps = 19/257 (7%) Query: 102 DLAMVQRGAFDQLGASHAICNVVYGAQAVFDSYMAADFCKAINDWIAAEWLARDKRLRAS 161 DL + D G + ++ VF+ +A + ND ++ R Sbjct: 57 DLGAGRIAHMDATGIDIQVISITSPGVQVFEPELATTLARQANDQLSEAIKNHPDRFAGL 116 Query: 162 IVVPIQAPDLAVEEIERRAGDNRFVSVLVPSQ-GETLLGRRHYWPIYQAAEKYKLPIAIH 220 V Q+P A EIER VL+ S G L + YW I+ AAE PI +H Sbjct: 117 AAVAPQSPQTAALEIERAIQKLGLCGVLINSHTGGEYLDDQKYWEIFAAAEANSAPIYLH 176 Query: 221 AGSAYRGAPSSIGWPSYRYEYYLAEAQAFQAQT----LSLIYEGVFGKYPGLTVVLMESG 276 + +P I P Y Y A F +T L LI GVF +P LT+VL G Sbjct: 177 PRTP---SPEMIS-PFLDYGLYFA-GWGFTVETATHGLRLIMGGVFDHFPKLTIVLGHMG 231 Query: 277 VSWLPAFMWRANKTWRGVRVE---VPWVEREPAAIIRDHFRVTMQPFDAPLDPTSVADII 333 LP ++ R + + ++VE VP + R P+ +DHF +T P ++ ++ Sbjct: 232 -EGLPYWLQRLDNRY-SLQVEIGAVPKMPRLPSEYFKDHFLITT---SGVCSPPALRHVL 286 Query: 334 DQIGSDKMFLFASDYPH 350 + +GSD + LFA+DYP+ Sbjct: 287 EVLGSDAV-LFAADYPY 302 >AGc4595 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 327 Score = 72.8 bits (177), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 76/289 (26%), Positives = 119/289 (41%), Gaps = 46/289 (15%) Query: 96 GSKPGSDLAMVQRGAFD------QLGASHAICNVVY-----GAQAVFDSYMAADFCKAIN 144 G PG +Q D +L +H I ++ QA+ D A + + N Sbjct: 22 GFVPGDCWTELQHRLLDIQDTRLKLMDAHGIETMILSLNAPAVQAIPDKTKAIEIARRAN 81 Query: 145 DWIAAEWLARDKRLRASIVVPIQAPDLAVEEIERRAGDNRFVSVLVPSQGETLLGRR--- 201 D +A E R R A +P+Q PD A +E+ R D FV LV + G+ Sbjct: 82 DALAEECAKRPDRFLAFAALPLQDPDAATQELRRCVNDLGFVGALVNGFSQEGDGQTPLY 141 Query: 202 ----HYWPIYQAAEKYKLPIAIHA-------GSAYRGAPSSIGWPSYRYEYYLAEAQAFQ 250 Y P + EK +P +H Y G P +G P++ + AQ Sbjct: 142 YDLPQYRPFWGEVEKLDVPFYLHPRNPLPQDSRIYDGHPWLLG-PTWAF------AQETA 194 Query: 251 AQTLSLIYEGVFGKYPGLTVVLMESGVSWLPAFMWRANKTWRGVRVEVPWVEREPAA--- 307 L L+ G+F ++P L ++L G LP MWR + R V++P R PA Sbjct: 195 VHALRLMASGLFDEHPRLDIILGHMGEG-LPYMMWRIDH--RNAWVKLP--PRYPAKRRF 249 Query: 308 --IIRDHFRVTMQPFDAPLDPTSVADIIDQIGSDKMFLFASDYPHWQFD 354 ++F +T ++ D I +IG+D++ LF++D+P D Sbjct: 250 VDYFNENFHITTS---GNFRTQTLIDAILEIGADRI-LFSTDWPFENID 294