BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0104.0066.p (505 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Rv0214 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 325 2e-88 MT0224 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 325 2e-88 CC0944 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 288 2e-77 CC0966 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 238 3e-62 Rv1427c [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 231 3e-60 >Rv0214 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Mtu Mycobacterium tuberculosis H37Rv Length = 537 Score = 325 bits (833), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 206/512 (40%), Positives = 279/512 (54%), Gaps = 52/512 (10%) Query: 9 ISYGDIHARIARAAGGFRALGVQGGKPVGMMLRNDFALFEVVAASAALGSPVVPINWHLK 68 IS+ ++ AR R A FR G++ V +++ N+ + V+ A+ G VPIN HL Sbjct: 55 ISFDELEARANRLAHWFRQAGLREDDVVAILMENNEHVHAVMWAARRSGLYYVPINTHLT 114 Query: 69 ADEVAYILSDSGADTLV--------CHADLLPQIRDGLPPAIKLLVVTTPPEIAAAYGVA 120 A E AYI+ +SGA +V CH L + GLP + L A G+ Sbjct: 115 ASEAAYIVDNSGAKAIVGSAALRETCHG-LAEHLPGGLPDLLML----------AGGGLV 163 Query: 121 PALTRVPDGMTDWDVWRDKHQPLTDAPIG----GAPMFYTSGTTGLPKGVKRKPMPSEQQ 176 +T P+ + D QP D PI G + Y+SGTTG PKG+KR+ +P Sbjct: 164 GWMT-YPECVAD--------QP--DTPIEDEREGDLLQYSSGTTGRPKGIKRE-LPHVSP 211 Query: 177 AAAARVGAITYGVKPNEKQVILMNGPMYHSAPHSYGMLAFRNGCVIVLEPRFDPEDLLQL 236 AA + + V L PMYH+AP + M A G V+ +FD E L Sbjct: 212 DAAPGMMPALLDFWMDADSVYLSPAPMYHTAPSVWTMSALAAGVTTVVMEKFDAEGALDA 271 Query: 237 IERHRVTHMHMVPTMFVRLLRLPEETRRRYDLSSLRFIVHGAAPCPVDVKRAMIEWWGPV 296 I+R+RVTH VP MFVR+L+LPE R YD+SSLR ++H AAPCPV +K MI WWGP+ Sbjct: 272 IQRYRVTHAQFVPAMFVRMLKLPEAVRNSYDMSSLRRVIHAAAPCPVQIKEQMIHWWGPI 331 Query: 297 INEYLGSTETGIPVWHSSAEALAKPGTVGRAIEGGIVKIFRPDGTQCDVGEPGEIFMRQK 356 I+EY S+E ++ + L PG+VG+ I+GG V I DG++ +PGEI+ Sbjct: 332 IDEYYASSEASGSTLITAEDWLTHPGSVGKPIQGG-VHIVGADGSELPPNQPGEIYFEGG 390 Query: 357 LISDFDYHGN-AKARAEADHDGLISVGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEI 415 F+Y + AK A + G ++VGDVGYLD DGYLFL R+ M+ISGGVNIYP E Sbjct: 391 Y--PFEYLNDPAKTAASRNKHGWVTVGDVGYLDDDGYLFLTGRRHHMIISGGVNIYPQEA 448 Query: 416 ENALIGMAGVRDCAVFGVPDDEYGERLFACVEPEAGSTLSVAAIQD--------FLRDKL 467 EN L+ V D AVFGVPDDE G+R+ A V+ T+ A D +LRD+L Sbjct: 449 ENLLVAHPKVLDAAVFGVPDDEMGQRVMAAVQ-----TVDSADANDQFAGELLAWLRDRL 503 Query: 468 ANFKVPKDIRFMDALPREATGKIFKRKLRDLY 499 ++FK P+ I F LPR TGK++K L + Y Sbjct: 504 SHFKCPRSIAFEPQLPRTDTGKLYKSGLVEKY 535 >MT0224 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II MtC Mycobacterium tuberculosis CDC1551 Length = 537 Score = 325 bits (833), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 206/512 (40%), Positives = 279/512 (54%), Gaps = 52/512 (10%) Query: 9 ISYGDIHARIARAAGGFRALGVQGGKPVGMMLRNDFALFEVVAASAALGSPVVPINWHLK 68 IS+ ++ AR R A FR G++ V +++ N+ + V+ A+ G VPIN HL Sbjct: 55 ISFDELEARANRLAHWFRQAGLREDDVVAILMENNEHVHAVMWAARRSGLYYVPINTHLT 114 Query: 69 ADEVAYILSDSGADTLV--------CHADLLPQIRDGLPPAIKLLVVTTPPEIAAAYGVA 120 A E AYI+ +SGA +V CH L + GLP + L A G+ Sbjct: 115 ASEAAYIVDNSGAKAIVGSAALRETCHG-LAEHLPGGLPDLLML----------AGGGLV 163 Query: 121 PALTRVPDGMTDWDVWRDKHQPLTDAPIG----GAPMFYTSGTTGLPKGVKRKPMPSEQQ 176 +T P+ + D QP D PI G + Y+SGTTG PKG+KR+ +P Sbjct: 164 GWMT-YPECVAD--------QP--DTPIEDEREGDLLQYSSGTTGRPKGIKRE-LPHVSP 211 Query: 177 AAAARVGAITYGVKPNEKQVILMNGPMYHSAPHSYGMLAFRNGCVIVLEPRFDPEDLLQL 236 AA + + V L PMYH+AP + M A G V+ +FD E L Sbjct: 212 DAAPGMMPALLDFWMDADSVYLSPAPMYHTAPSVWTMSALAAGVTTVVMEKFDAEGALDA 271 Query: 237 IERHRVTHMHMVPTMFVRLLRLPEETRRRYDLSSLRFIVHGAAPCPVDVKRAMIEWWGPV 296 I+R+RVTH VP MFVR+L+LPE R YD+SSLR ++H AAPCPV +K MI WWGP+ Sbjct: 272 IQRYRVTHAQFVPAMFVRMLKLPEAVRNSYDMSSLRRVIHAAAPCPVQIKEQMIHWWGPI 331 Query: 297 INEYLGSTETGIPVWHSSAEALAKPGTVGRAIEGGIVKIFRPDGTQCDVGEPGEIFMRQK 356 I+EY S+E ++ + L PG+VG+ I+GG V I DG++ +PGEI+ Sbjct: 332 IDEYYASSEASGSTLITAEDWLTHPGSVGKPIQGG-VHIVGADGSELPPNQPGEIYFEGG 390 Query: 357 LISDFDYHGN-AKARAEADHDGLISVGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEI 415 F+Y + AK A + G ++VGDVGYLD DGYLFL R+ M+ISGGVNIYP E Sbjct: 391 Y--PFEYLNDPAKTAASRNKHGWVTVGDVGYLDDDGYLFLTGRRHHMIISGGVNIYPQEA 448 Query: 416 ENALIGMAGVRDCAVFGVPDDEYGERLFACVEPEAGSTLSVAAIQD--------FLRDKL 467 EN L+ V D AVFGVPDDE G+R+ A V+ T+ A D +LRD+L Sbjct: 449 ENLLVAHPKVLDAAVFGVPDDEMGQRVMAAVQ-----TVDSADANDQFAGELLAWLRDRL 503 Query: 468 ANFKVPKDIRFMDALPREATGKIFKRKLRDLY 499 ++FK P+ I F LPR TGK++K L + Y Sbjct: 504 SHFKCPRSIAFEPQLPRTDTGKLYKSGLVEKY 535 >CC0944 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Ccr Caulobacter vibrioides Length = 512 Score = 288 bits (737), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 185/507 (36%), Positives = 261/507 (51%), Gaps = 29/507 (5%) Query: 2 IISGDRQI-SYGDIHARIARAAGGFRALGVQGGKPVGMMLRNDFALFEVVAASAALGSPV 60 I++G ++ +Y + AR + A FR+LG+ G + +++ N+ FEV A+ G Sbjct: 17 IMAGSGEVVTYAQLDARSNQGAHLFRSLGLVPGDVIAILMENNARFFEVAWAAQRSGLYF 76 Query: 61 VPINWHLKADEVAYILSDSGADTLVCHADLLPQIRD--GLPPAIKLLVVTTPPEIAAAYG 118 V I+ L A E YIL D GA + + P I + L P + L V A+G Sbjct: 77 VCISTKLTASEAEYILQDCGAKVFITSTAMGPMIDEIAALVPGLSLFTV------GGAHG 130 Query: 119 VAPALTRVPDGMTDWDVWRDKHQPLTDAPIGGAPMFYTSGTTGLPKGVK--RKPMPSEQQ 176 +G P+ D G M Y+SGTTG PKGVK P + Sbjct: 131 PYADFVAAREGRPT--------TPIADES-AGTDMLYSSGTTGRPKGVKPALSGGPIDAP 181 Query: 177 AAAARVGAITYGVKPNEKQVILMNGPMYHSAPHSYGMLAFRNGCVIVLEPRFDPEDLLQL 236 A + +G + + L P+YH+AP + M + G +++ +F+PE L L Sbjct: 182 NALQMMAQGLFGFSGD--SIYLSPAPLYHAAPLRWCMTVHKLGGTVIVMEKFEPETALAL 239 Query: 237 IERHRVTHMHMVPTMFVRLLRLPEETRRRYDLSSLRFIVHGAAPCPVDVKRAMIEWWGPV 296 IE+HRV VPT FVR+L+LPE R +YD+SS++ VH AAPCP+ VK MI WWGPV Sbjct: 240 IEQHRVNCGQFVPTHFVRMLKLPEAVRAKYDVSSMKSAVHAAAPCPIPVKEQMIAWWGPV 299 Query: 297 INEYLGSTETGIPVWHSSAEALAKPGTVGRAIEGGIVKIFRPDGTQCDVGEPGEIFMRQK 356 I EY TE W +S L G+VG+A+ G + +I G G ++ Sbjct: 300 IFEYYAGTEGNGFCWINSQNWLTHKGSVGQAVLGEL-RICDEAGDPVPPRTEGTVYFANG 358 Query: 357 LISDFDYHGNAKARAEA-DHDGLISVGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEI 415 +YH AE+ + G ++GDVG+ D DG+L+L DRK M+ISGGVNIYP EI Sbjct: 359 --PAVNYHNAPDKTAESYNKHGWTTLGDVGWADEDGFLYLTDRKSFMIISGGVNIYPQEI 416 Query: 416 ENALIGMAGVRDCAVFGVPDDEYGERLFACVEPEAGSTLSVAAIQD---FLRDKLANFKV 472 EN LI V D AV G P +E GE++ A ++P VA ++ F R L++ K Sbjct: 417 ENLLITHPKVADAAVVGAPHEEMGEQVVAVIQPMDLGDDQVALAEELMAFARANLSHVKS 476 Query: 473 PKDIRFMDALPREATGKIFKRKLRDLY 499 P+ I FM LPR TGK++KR +RD Y Sbjct: 477 PRRIDFMAELPRHPTGKLYKRLIRDAY 503 >CC0966 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Ccr Caulobacter vibrioides Length = 530 Score = 238 bits (607), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 169/507 (33%), Positives = 248/507 (48%), Gaps = 26/507 (5%) Query: 7 RQISYGDIHARIARAAGGFRALGVQGGKPVGMMLRNDFALFEVVAASAALGSPVVPINWH 66 R+ ++ + + A A G+ G + + +N FE++ +A G PI W Sbjct: 35 RETTFAQLDRHTNQVANALLAAGLSTGDRIAYVGKNSDHYFELLLGAAKAGVVTTPIGWR 94 Query: 67 LKADEVAYILSDSGADTLVCHADLLPQIRDGLPPAI--KLLVVTTPPEIAAAYGVAPALT 124 L A E+AYI+ DS A + +L+ + D + + + +V+ E A Y Sbjct: 95 LAAPEIAYIVGDSEAKLVFVGRELIGHV-DAVAAELTHRPVVIAMEAEGAGDY------- 146 Query: 125 RVPDGMTDWDVWRDKHQPLTDAPIGGAPMFYTSGTTGLPKGVKRKPMPSEQQAAAARVGA 184 + +G D D H+P+ + I A YTSGTTG PKG M + R A Sbjct: 147 QTFEGWRDAASDVDPHKPIQISDI--AIQLYTSGTTGRPKGA----MLTHHNLLGMRREA 200 Query: 185 ITYGVKPNE---KQVILMNGPMYHSAPHSYGMLAFRNGCVIVLEPRFDPEDLLQLIERHR 241 ++ N+ V L+ P+ H +G++ NG V+ FDP +L IE+ R Sbjct: 201 AKNPLEWNQWGPSDVSLVAMPVAHIGGTGWGLVGLINGAKGVVAREFDPTKVLDFIEKDR 260 Query: 242 VTHMHMVPTMFVRLLRLPEETRRRYDLSSLRFIVHGAAPCPVDVKRAMIEWWGPVINEYL 301 ++ M MVP ++RLP R D S L I++GAAP P+D+ R +E +G + Sbjct: 261 ISKMFMVPAALQIVVRLPRA--REVDYSRLTHILYGAAPIPLDLLRECMEVFGCGFVQQY 318 Query: 302 GSTETGIPVWHSSAEALAKPGT-----VGRAIEGGIVKIFRPDGTQCDVGEPGEIFMRQK 356 G TET V + E G G + G +KI G GE+ +R Sbjct: 319 GMTETTGTVVYLPPEDHDPAGNKRMRAAGLPMPGVELKIIDEAGKSLPPNTVGEVAVRSS 378 Query: 357 LISDFDYHGNAKARAEADHDGLISVGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIE 416 + + D DG + GD GYLD DGYLF+ DR +DM+ISGG NIYPAE+E Sbjct: 379 ANMAGYWKLDEATAKTMDADGWLRTGDAGYLDEDGYLFIHDRVKDMIISGGENIYPAEVE 438 Query: 417 NALIGMAGVRDCAVFGVPDDEYGERLFACVEPEAGSTLSVAAIQDFLRDKLANFKVPKDI 476 +A+ G V + AV GVPDD++GE + A V P+ G T I F R ++A+FK PK + Sbjct: 439 SAVYGHPHVAEVAVIGVPDDKWGEAVKAVVAPKPGVTPDADDIIAFARTRIAHFKAPKSV 498 Query: 477 RFMDALPREATGKIFKRKLRDLYREGQ 503 F+ ALPR A+GKI +R+LR Y EG+ Sbjct: 499 DFIPALPRNASGKILRRELRAPYWEGR 525 >Rv1427c [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Mtu Mycobacterium tuberculosis H37Rv Length = 535 Score = 231 bits (589), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 153/467 (32%), Positives = 228/467 (48%), Gaps = 30/467 (6%) Query: 34 KPVGMMLRNDFALFEVVAASAALGSPVVPINWHLKADEVAYILSDSGADTLVCHADLLPQ 93 + VG+M RN + + A +G+ ++ +N +A +++ G DT+V + Sbjct: 90 RVVGIMCRNHRGFVDALLAVNRIGAHILLLNTSFAGPALAEVVTREGVDTVVYDEEFSAT 149 Query: 94 IRDGL---PPAIKLLVVTTPP-EIAAAYGVAPALTRVPDGMTDWDVWRDKHQPLTDAPIG 149 + L P A +++ T ++ VA R P+ Sbjct: 150 VDRALAEKPQATRIVAWTDEDHDLTVEKLVAAHAGRRPEHTGSH---------------- 193 Query: 150 GAPMFYTSGTTGLPKGVKRKPMPSEQQAAAARVGAITYGVKPNEKQVILMNGPMYHSAPH 209 G + TSGTTG PKG + + AI ++V ++ PM+H+ Sbjct: 194 GKVILLTSGTTGTPKGAR------HSGGGIGTLKAILDRTPWRAEEVTVIVAPMFHAWGF 247 Query: 210 SYGMLAFRNGCVIVLEPRFDPEDLLQLIERHRVTHMHMVPTMFVRLLRLPEETRRRYDLS 269 S +LA C IV RFDPE L LI+RH T + +VP MF R++ LP E R RYD Sbjct: 248 SQLVLASSLACTIVTRRRFDPEATLDLIDRHHATGLVVVPVMFDRIMDLPAEIRNRYDGR 307 Query: 270 SLRFIVHGAAPCPVDVKRAMIEWWGPVINEYLGSTETGIPVWHSSAEALAKPGTVGRAIE 329 SLRF + DV A ++ +G VI +TE G+ + A+ P T GR E Sbjct: 308 SLRFAAASGSRMRPDVVIAFMDQFGDVIYNNYNATEAGMIATATPADLRTAPDTAGRPAE 367 Query: 330 GGIVKIFRPDGTQCDVGEPGEIFMRQKLISDFDYHGNAKARAEADHDGLISVGDVGYLDA 389 G ++I T+ GE G I++R +D + G A+ H G +S GDVGYLD Sbjct: 368 GTEIRILDQQFTEVPTGEVGTIYVR----NDSQFDGYTSGAAKDFHAGFMSSGDVGYLDE 423 Query: 390 DGYLFLCDRKRDMVISGGVNIYPAEIENALIGMAGVRDCAVFGVPDDEYGERLFACVEPE 449 +G LF+ R +M++SGG NIYP E+E L V + AV GV D +YG+RL A V + Sbjct: 424 NGRLFVVGRDDEMIVSGGENIYPIEVEKTLATHPDVAEAAVIGVDDQQYGQRLAAFVVLK 483 Query: 450 AGSTLSVAAIQDFLRDKLANFKVPKDIRFMDALPREATGKIFKRKLR 496 G + + ++ +RD LAN+KVP+DI +D LPR TGKI + +L+ Sbjct: 484 PGVSATPETLKQHVRDNLANYKVPRDIAVLDELPRGITGKILRTELQ 530