BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0102.0006.p (391 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl1631 [L] COG3569 Topoisomerase IB Atu Agrobacterium tumefacie... 189 8e-48 PA2244 [L] COG3569 Topoisomerase IB Pae Pseudomonas aeruginosa 187 3e-47 SMb21445 [L] COG3569 Topoisomerase IB Sme Sinorhizobium meliloti 182 1e-45 DR0690 [L] COG3569 Topoisomerase IB Dra Deinococcus radiodurans 162 9e-40 mll2873 Non COG 130 5e-30 >AGl1631 [L] COG3569 Topoisomerase IB Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 338 Score = 189 bits (481), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 111/295 (37%), Positives = 165/295 (55%), Gaps = 7/295 (2%) Query: 61 LGLRLGDQNELTIRRIKRGKTYSFVRANGSRVRDARTIRRLHAMAVPPAYRQVRYSSDPS 120 +GL + E I R KRG+ + + G + D+ ++R+ ++ VPPAYR V DP+ Sbjct: 12 IGLAYVNDQEPGIAREKRGRGFCYRLPGGDLLSDSVELKRIKSLGVPPAYRDVWICIDPA 71 Query: 121 SHLQAVGRDAAGRLQYRYHADWEKVREQRKAHRLAKLVAALPKIRRKVSAFLSGDEPTRE 180 HLQA G DA GR QYRYH DW +R + K +L ALP IRR+ A + + +E Sbjct: 72 GHLQATGFDARGRKQYRYHPDWHALRGETKFFQLKSFGKALPAIRRRAMADIGKQDHGQE 131 Query: 181 FALSAVIELIARTAIRPGNESYARLNGTRGATTLLKSNVTLEDDSLVLTFKAKGGKAVRK 240 L+A+ L+ +R GN SY NGT GATTLLK +V+ +++ L F AKGG+ V++ Sbjct: 132 MTLAALTLLLDAAYLRVGNRSYLETNGTYGATTLLKRHVSF-GETIELRFAAKGGQKVKR 190 Query: 241 ECDAAKLVRAIGILRGVPGKRMFQYYDRSGVVRAASTTAVNAFLREIAGIKISLKDFRTL 300 + +L + + + +PGK +F + D V + ++ +NA+L I G IS K FRT Sbjct: 191 QLRHPRLQKILEEIADLPGKELFVWQDSENRVHSVDSSDLNAYLSGIGGQGISAKTFRTW 250 Query: 301 MAS-AVVVESLSRITPATSQRGRKRQVLDAIRAAADQLSNTPAICRKSYVHDTIV 354 + A ++ + G K + +AAA +LSNTPAICRKSYVH ++ Sbjct: 251 GGTLAAFCNAMEHVAA-----GEKPSIKGMCQAAAAELSNTPAICRKSYVHPAVL 300 >PA2244 [L] COG3569 Topoisomerase IB Pae Pseudomonas aeruginosa Length = 333 Score = 187 bits (475), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 117/324 (36%), Positives = 165/324 (50%), Gaps = 17/324 (5%) Query: 62 GLRLGDQNELTIRRIKRGKTYSFVRANGSRVRDARTIRRLHAMAVPPAYRQVRYSSDPSS 121 GL D + IRR ++GK + + A+G R+ DA IRRL +A+PPAYR+V DP+ Sbjct: 14 GLCYVDDRQPGIRRRRQGKRFVYFDADGQRIGDAEEIRRLDKLAIPPAYREVWICPDPNG 73 Query: 122 HLQAVGRDAAGRLQYRYHADWEKVREQRKAHRLAKLVAALPKIRRKVSAFLSGDEPTREF 181 HLQA GRDA GR QYRYH W +VR+ K RL + ALP++RR + A L R+ Sbjct: 74 HLQATGRDARGRKQYRYHPRWREVRDADKYERLLRFGQALPRLRRHIDAQLRLPGLGRDK 133 Query: 182 ALSAVIELIARTAIRPGNESYARLNGTRGATTLLKSNVTLEDDSLVLTFKAKGGKAVRKE 241 ++ + L+ T IR GN YAR N + G TTL +V + + FK K G Sbjct: 134 VVALAVALLDATLIRVGNHRYARDNRSYGLTTLRTRHVDVNGSRIRFRFKGKSGIEHDVS 193 Query: 242 CDAAKLVRAIGILRGVPGKRMFQYYDRSGVVRAASTTAVNAFLREIAGIKISLKDFRTLM 301 + +L R + +PG+ + QY D G + +N +LR G S KD+RT Sbjct: 194 LEHPRLARVLRRCLELPGQDLLQYLDEDGQPHRIGSHDINEYLRRHTGEDFSAKDYRTWA 253 Query: 302 ASAVVVESLSRITPATSQRGRKRQVLDAIRA-AADQLSNTPAICRKSYVHDTIVTAFEDG 360 SA+ +E L R L A+ A A +L N+ AICR+ Y+H I+ A++ Sbjct: 254 GSALALERLRRAEADG---------LGAVVAEVAAELGNSVAICRQCYIHPAIIEAYQ-- 302 Query: 361 ILERFAATMGGYRTQSKREQLLAQ 384 A +G R KR L A+ Sbjct: 303 -----AGQLGQLRRARKRRWLSAE 321 >SMb21445 [L] COG3569 Topoisomerase IB Sme Sinorhizobium meliloti Length = 351 Score = 182 bits (461), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 106/300 (35%), Positives = 165/300 (55%), Gaps = 8/300 (2%) Query: 57 LARDLGLRLGDQNELTIRRIKRGKTYSFVRANGSRVRDARTIRRLHAMAVPPAYRQVRYS 116 + + GL +E IRR +RGK + + +GS V D R+ A+ +PPAY V Sbjct: 1 MPEETGLVYVSDSEPGIRRQRRGKGFVYRMPDGSIVTDPSIKSRIAALGLPPAYDNVWIC 60 Query: 117 SDPSSHLQAVGRDAAGRLQYRYHADWEKVREQRKAHRLAKLVAALPKIRRKVSAFLSGDE 176 + HLQA G DA GR QYRYH++W+ +R K +L + ALPKIRR + + GD Sbjct: 61 LEERGHLQATGYDARGRKQYRYHSEWQALRSADKFAQLVEFGKALPKIRRTIRRHMQGDV 120 Query: 177 PTREFALSAVIELIARTAIRPGNESYARLNGTRGATTLLKSNVTLEDDSLVLTFKAKGGK 236 + L+A++ L+ +R GN++Y + NG+ GATTLLK ++ L D + L F KGGK Sbjct: 121 ENMQTVLAALVALLDEAHLRTGNQAYVQANGSYGATTLLKRHLRLGDGFIELKFIGKGGK 180 Query: 237 AVRKECDAAKLVRAIGILRGVPGKRMFQYYDRSGVVRAASTTAVNAFLREIAGIKISLKD 296 V++ KL + + + +PG+++F + D + V+R + +N +L ++AG +S K Sbjct: 181 RVQRLLRRPKLQQLLEEIADLPGRQLFVWKDENDVLRPVDSGRLNRYLSDVAGTAVSAKT 240 Query: 297 FRTLMASAVVVESLSRITPATS--QRGRKRQVLDAIRAAADQLSNTPAICRKSYVHDTIV 354 FRT +L+ T A + ++G + AAA L NTPAI R SY+H ++ Sbjct: 241 FRTWGG------TLAAFTAARTSIEQGEWPTIKQMSEAAASVLHNTPAISRSSYIHPDVL 294 >DR0690 [L] COG3569 Topoisomerase IB Dra Deinococcus radiodurans Length = 346 Score = 162 bits (411), Expect = 9e-40, Method: Compositional matrix adjust. Identities = 101/284 (35%), Positives = 155/284 (54%), Gaps = 6/284 (2%) Query: 80 KTYSFVRANGSRVRDARTIRRLHAMAVPPAYRQVRYSSDPSSHLQAVGRDAAGRLQYRYH 139 + + +V +G+ DA + R+ +AVPPAY+ V S D + LQA GRDAAGRLQYRYH Sbjct: 21 QKFRYVHPDGTPYTDADGLARIARLAVPPAYQDVYVSPDAENELQAFGRDAAGRLQYRYH 80 Query: 140 ADWEKVREQRKAHRLAKLVAALPKIRRKVSAFL--SGDEPTREFALSAVIELIARTAIRP 197 D+ + +K RL + ALP ++ +A L SG P + AL + +AR R Sbjct: 81 PDFVQAGALKKWQRLTRFAGALPTLKVATTADLRASGLPPRKVMALMTRLLHVAR--FRV 138 Query: 198 GNESYARLNGTRGATTLLKSNVTLEDDSLVLTFKAKGGKAVRKECDAAKLVRAIGILRGV 257 G++ YAR + T G +TL + +V ++ +++ FK K G + K L + L + Sbjct: 139 GSDIYARQHKTYGLSTLRQRHVVVDGNTVTFRFKGKHGVSQHKATSDRTLAANMQKLLDL 198 Query: 258 PGKRMFQYYDRSGVV-RAASTTAVNAFLREIAGIKISLKDFRTLMASAVVVESLSRITPA 316 PG +FQ D G R +T +NA+LRE+ G + KDFRT + + E L++ Sbjct: 199 PGPWLFQTVDAGGGERRRIHSTELNAYLREVIG-PFTAKDFRTWGGTLLAAEYLAQQGTE 257 Query: 317 TSQRGRKRQVLDAIRAAADQLSNTPAICRKSYVHDTIVTAFEDG 360 +S+R K+ ++D ++ AD L NTPA+ R SY+ I + DG Sbjct: 258 SSERQAKKVLVDCVKFVADDLGNTPAVTRGSYICPVIFDRYLDG 301 >mll2873 Non COG Length = 288 Score = 130 bits (327), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 88/240 (36%), Positives = 121/240 (50%), Gaps = 11/240 (4%) Query: 39 PSTLKLDAPAAIRTTVEELARDLGLRLGDQNELTIRRIKRGKTYSFVRANGSRVRDARTI 98 P D AA + + A L E IRR+K GK +S+ +G V DA Sbjct: 8 PPGRSTDGDAAPKNGAQSAAEHAALTYVSDAEPGIRRLKTGKGFSYKGPDGKAVSDAARA 67 Query: 99 RRLHAMAVPPAYRQVRYSSDPSSHLQAVGRDAAGRLQYRYHADWEKVREQRKAHRLAKLV 158 R + A+ +PPA+ V S D + H+QA GRD GR QYRYH W K R+ K L Sbjct: 68 R-IEAIVIPPAWTDVWISLDANGHIQATGRDQRGRKQYRYHPQWAKERDGAKYSSLVAFA 126 Query: 159 AALPKIRRKVSAFLSGDEPTREFALSAVIELIARTAIRPGNESYARLNGTRGATTLLKSN 218 +L +RR + + L E ++AV+ L+ T IR GN +YAR N + G TTL + Sbjct: 127 ESLASLRRTIDSDLRRHGLPFERVVAAVVWLLDNTMIRVGNAAYARDNQSFGLTTLRDRH 186 Query: 219 VTLEDDSLVLTFKAKGGKAVRKECDAAKLV--RAIGILRG---VPGKRMFQYYDRSGVVR 273 V ++ SL FK K GK + +LV R GI+RG +PG+++FQY D G R Sbjct: 187 VDIKGSSLRFAFKGKSGKEWK-----LRLVDRRIAGIVRGAQDLPGQKLFQYLDEDGSRR 241