BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0101.0028.p (211 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2949 [U] COG2095 Multiple antibiotic transporter Rso Ralstoni... 121 9e-28 YPO2181 [U] COG2095 Multiple antibiotic transporter Ype Yersinia... 112 4e-25 VC2035 [U] COG2095 Multiple antibiotic transporter Vch Vibrio ch... 106 3e-23 STM1748 [U] COG2095 Multiple antibiotic transporter Sty Salmonel... 106 4e-23 ZychE [U] COG2095 Multiple antibiotic transporter EcZ Escherichi... 100 2e-21 >RSc2949 [U] COG2095 Multiple antibiotic transporter Rso Ralstonia solanacearum Length = 207 Score = 121 bits (304), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 69/195 (35%), Positives = 107/195 (54%), Gaps = 1/195 (0%) Query: 13 LYALTSPVGAIPVFLAITRQARSANIHRIIVLACTAVAVFFVGAAVLGKQILGFFNVGLD 72 L AL +P+GAIP F+++T R I A +VA+ +A+LG+QI+GFF + Sbjct: 10 LLALINPIGAIPFFISLTESQTDEEKRRTIKTASVSVALVIGISALLGEQIIGFFGFSVG 69 Query: 73 DFRIAGGLLALVIALEMFQAQYGKFLQSTSGVDSSEVDIHGMAITPFAFPLLVGPAQVGI 132 ++ GG++ ++IAL M AQ + + D + V +A+ P A PLL GP + Sbjct: 70 SLQVGGGIIMIMIALNMLNAQTSRTKATPEEEDEAGVR-ASIAVVPLAIPLLTGPGSIST 128 Query: 133 MITLSNDNPGWLAKALLAAASIIATFLIGLTLWTAAAIERFLGKTGINVMTRTMALIVAA 192 +I + W LA ++ ++ + L A I R +G+TGINV TR M LI+AA Sbjct: 129 VIVYAGKTQHWYELLFLAGVGVLIALVVWIVLRAAEPIARVIGRTGINVGTRLMGLILAA 188 Query: 193 IGVHFIMTGLRSELP 207 + V FI+ GL++ LP Sbjct: 189 LAVEFIVDGLKTLLP 203 >YPO2181 [U] COG2095 Multiple antibiotic transporter Ype Yersinia pestis Length = 214 Score = 112 bits (281), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 71/204 (34%), Positives = 109/204 (53%), Gaps = 5/204 (2%) Query: 6 FVGITMLLYALTSPVGAIPVFLAITRQARSANIHRIIVLACTAVAVFFVGAAVLGKQILG 65 FVG L+AL +PVG +PVF+++T +A + + A +VA+ + LG IL Sbjct: 15 FVG----LFALVNPVGILPVFISMTSYQAAAGRDKTNLTANLSVAIILWISLFLGDAILT 70 Query: 66 FFNVGLDDFRIAGGLLALVIALEMFQAQYGKFLQSTSGVDSSEVDIHGMAITPFAFPLLV 125 F + +D FRIAGG+L + IA+ M + G+ Q+ + V + + P A PL+ Sbjct: 71 IFGISIDSFRIAGGILVVSIAMSMISGKLGEDKQNKQEKTETAVR-ESIGVVPLALPLMA 129 Query: 126 GPAQVGIMITLSNDNPGWLAKALLAAASIIATFLIGLTLWTAAAIERFLGKTGINVMTRT 185 GP + I S+ W L A + F L A + R LG+TGINV+TR Sbjct: 130 GPGAISSTIVWSSRYHSWQNLLGLTVAIALFAFCCWLLFRAAPLLVRLLGQTGINVITRI 189 Query: 186 MALIVAAIGVHFIMTGLRSELPGL 209 M L++ A+G+ FI+TG+++ PGL Sbjct: 190 MGLLLMALGIEFIVTGIKAIFPGL 213 >VC2035 [U] COG2095 Multiple antibiotic transporter Vch Vibrio cholerae Length = 212 Score = 106 bits (265), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 71/198 (35%), Positives = 105/198 (53%), Gaps = 1/198 (0%) Query: 13 LYALTSPVGAIPVFLAITRQARSANIHRIIVLACTAVAVFFVGAAVLGKQILGFFNVGLD 72 L A +PVG +PVF+++T ++ A AVA+ + A + G+ +L F++ LD Sbjct: 16 LVAAVNPVGIMPVFVSLTGHMTLEEKNKTAATANIAVAIILIIALLAGQMLLDLFSISLD 75 Query: 73 DFRIAGGLLALVIALEMFQAQYGKFLQSTSGVDSSEVDIHGMAITPFAFPLLVGPAQVGI 132 FR+AGGLL L IA M + G+ Q+ S + +A+ P A PL+ GP + Sbjct: 76 SFRVAGGLLLLSIAFSMMSGKLGEDKQNKQE-KSEYISREQIAVVPLAMPLMAGPGAISS 134 Query: 133 MITLSNDNPGWLAKALLAAASIIATFLIGLTLWTAAAIERFLGKTGINVMTRTMALIVAA 192 I + P L + + +F L +A I R LG+TGINV+TR M LI+ A Sbjct: 135 TIVYGSRYPNMLDTLGIILTVVAFSFCSWLLFRSAPYIVRLLGQTGINVITRIMGLILGA 194 Query: 193 IGVHFIMTGLRSELPGLA 210 +G+ FI GLR+ PGLA Sbjct: 195 LGIEFIANGLRNLFPGLA 212 >STM1748 [U] COG2095 Multiple antibiotic transporter Sty Salmonella typhimurium LT2 Length = 215 Score = 106 bits (264), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 71/202 (35%), Positives = 109/202 (53%), Gaps = 11/202 (5%) Query: 13 LYALTSPVGAIPVFLAITRQARSANIHRIIVLACTAVAVFFVGAAVLGKQILGFFNVGLD 72 L+AL +PVG IPVF+++T + ++ + A +VA+ + LG IL F + +D Sbjct: 18 LFALVNPVGIIPVFISMTSYQTATARNKTNLTANLSVAIILWSSLFLGDGILQLFGISID 77 Query: 73 DFRIAGGLLALVIALEMFQAQYGKFLQSTSGVDSSEVDI-HGMAITPFAFPLLVGPAQVG 131 FRIAGG+L + IA+ M + G+ Q+ + SE I + + P A PL+ GP + Sbjct: 78 SFRIAGGILVVTIAMSMISGKLGEDKQNKQ--EKSETAIRESIGVVPLALPLMAGPGAIS 135 Query: 132 IMITLSNDNPGWLAKALLAAASIIATFLIGLTLW----TAAAIERFLGKTGINVMTRTMA 187 I + + A L S +A L L W A + R LG+TGINV+TR M Sbjct: 136 STIVWGTR---YHSIAHLLGFS-VAIALFALCCWGVFRMAPWLVRLLGQTGINVITRIMG 191 Query: 188 LIVAAIGVHFIMTGLRSELPGL 209 L++ A+G+ FI+TG++ PGL Sbjct: 192 LLLMALGIEFIVTGIKGIFPGL 213 >ZychE [U] COG2095 Multiple antibiotic transporter EcZ Escherichia coli O157:H7 EDL933 Length = 215 Score = 100 bits (250), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 69/204 (33%), Positives = 108/204 (52%), Gaps = 15/204 (7%) Query: 13 LYALTSPVGAIPVFLAITRQARSANIHRIIVLACTAVAVFFVGAAVLGKQILGFFNVGLD 72 L+AL +PVG IPVF+++T +A ++ + A +VA+ + LG IL F + +D Sbjct: 18 LFALVNPVGIIPVFISMTSYQTAAARNKTNLTANLSVAIILWISLFLGDTILQLFGISID 77 Query: 73 DFRIAGGLLALVIALEMFQAQYGKFLQSTSGVDSSEVDI-HGMAITPFAFPLLVGPAQVG 131 FRIAGG+L + IA+ M + G+ Q+ + SE + + + P A PL+ GP + Sbjct: 78 SFRIAGGILVVTIAMSMISGKLGEDKQNKQ--EKSETAVRESIGVVPLALPLMAGPGAIS 135 Query: 132 IMITLSN--DNPGWLAKALLAAASIIATFLIGLTLW----TAAAIERFLGKTGINVMTRT 185 I + +L +A A L L W A + R L +TGINV+TR Sbjct: 136 STIVWGTRYHSISYLFGFFVAIA------LFALCCWGLFRMAPWLVRVLRQTGINVITRI 189 Query: 186 MALIVAAIGVHFIMTGLRSELPGL 209 M L++ A+G+ FI+TG++ PGL Sbjct: 190 MGLLLMALGIEFIVTGIKGIFPGL 213