BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0100.0002.p (284 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the T... 208 1e-53 mlr7156 Non COG 129 9e-30 SMb21101 [R] COG3618 Predicted metal-dependent hydrolase of the ... 119 5e-27 AGl2610 [R] COG3618 Predicted metal-dependent hydrolase of the T... 115 1e-25 BMEI1718 [R] COG3618 Predicted metal-dependent hydrolase of the ... 113 5e-25 >AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 287 Score = 208 bits (530), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 114/270 (42%), Positives = 157/270 (58%), Gaps = 4/270 (1%) Query: 4 IDAHHHVWTLARADYGWLTPERGPIYRDFGLADLAPHLAAAGIEGTILVQAAPTEAETAF 63 IDAH H W + W P IYRDF DL+P L AAG+ GT+LVQ T A+T F Sbjct: 17 IDAHQHFWLMKDRAGQWPPPSLAAIYRDFLPTDLSPLLTAAGVSGTVLVQTMETAADTDF 76 Query: 64 LLDIAKGAEQVRGVVGWIDFDAADAAARIDAIAGRELLVGLRPMVQDIADDDWLLRPELA 123 +L++A+ + ++GVVGW+D +A+ A I A G+RPM+Q + D W+ L Sbjct: 77 MLELAENTDFIKGVVGWVDMKSAEVTAEIARRAKHPAFKGVRPMLQGMEDVAWIDDAALN 136 Query: 124 APLEAMARHDLVFDALVLPRHLQRLIQVVDRHPDLQFVLDHFGKPHLATGDIATWKDDIA 183 A +EA+ +H LVFDALVLP +L L+ +HP+L V+DH KP +ATG W+ ++A Sbjct: 137 AAVEALVKHGLVFDALVLPPNLPHLLSFASKHPELPVVIDHGAKPLIATGHYRDWRRNMA 196 Query: 184 SLATRSNVVCKLSGLATEAAPNWQVADLSEAVDHALACFGPQRMLWGSDWPVVNLAGGYG 243 +LA R NV CKLSGL TE A + + L FG R+++GSDWPV+ LAG + Sbjct: 197 ALAARPNVHCKLSGLLTERGEQRPEA-VRPYAETILELFGGDRVIFGSDWPVLRLAGDFQ 255 Query: 244 HWFAAAETLLADLSSDARAAIFGGNAARIY 273 W + + + AA+FGGNA R Y Sbjct: 256 GWLDFCRDI---VPAKDHAAVFGGNAIRFY 282 >mlr7156 Non COG Length = 297 Score = 129 bits (323), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 88/278 (31%), Positives = 134/278 (48%), Gaps = 12/278 (4%) Query: 4 IDAHHHVWTLARADYGWL--TPERGPIYRDFGLADLAPHLAAAGIEGTILVQA----APT 57 ID H H+ + Y WL P+ + RDF D A G+E + ++ A Sbjct: 21 IDTHLHLIDRSALRYPWLAGVPQ---LNRDFSYEDYATEAQRVGVERVLHMEVDVDPADI 77 Query: 58 EAETAFLLDIAKGA-EQVRGVVGWIDFDAADAAARIDAIAGRELLVGLRPMVQDIADDDW 116 E ETA + +++ A + G + + AD A ++ + G R ++ + DD Sbjct: 78 EVETARVEGLSRQAGSMLVGAIASCRPEDADFPAYLERQRTNPFVKGFRRVLH-VVPDDL 136 Query: 117 LLRPELAAPLEAMARHDLVFDALVLPRHLQRLIQVVDRHPDLQFVLDHFGKPHLATGDIA 176 ++ + L FD +VLP + + I + D PD+QFVLDH G P + G Sbjct: 137 SEGAVFRDNIKRLGGIGLTFDLVVLPHQIPKAIALADLAPDVQFVLDHCGVPDIKGGAEH 196 Query: 177 TWKDDIASLATRSNVVCKLSGLATEAAP-NWQVADLSEAVDHALACFGPQRMLWGSDWPV 235 W+ ++ +A R NV+ K+SG+ A P NW V L V+H +A FG R++WGSDWPV Sbjct: 197 PWRAHMSEIARRPNVMAKISGVVAYADPVNWTVGTLRPYVEHTIAAFGWDRIVWGSDWPV 256 Query: 236 VNLAGGYGHWFAAAETLLADLSSDARAAIFGGNAARIY 273 L GG W AA LL+ S R + GNA +++ Sbjct: 257 CTLGGGLSTWVAATHALLSGCSIAERDRLLSGNARKLW 294 >SMb21101 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Sme Sinorhizobium meliloti Length = 278 Score = 119 bits (299), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 78/275 (28%), Positives = 128/275 (46%), Gaps = 7/275 (2%) Query: 4 IDAHHHVWTLARADYGWLTPERGPIYRDFGLADLAPHLAAAGIEGTILVQA----APTEA 59 ID H H+ + +Y WL + RDF A A G+ ++ ++ A E Sbjct: 3 IDTHLHLIDKSALNYPWLAGVPA-LDRDFLYATYAAEAKRVGVAASLHMEVDVDPAEIEL 61 Query: 60 ETAFLLDIA-KGAEQVRGVVGWIDFDAADAAARIDAIAGRELLVGLRPMVQDIADDDWLL 118 ET + +A + ++G + + AA ++ + G R ++ + DD Sbjct: 62 ETREVARLAGEPGSLLKGAIAACRPEDEGFAAYLERQEENAFVKGFRRVLH-VVTDDLSE 120 Query: 119 RPELAAPLEAMARHDLVFDALVLPRHLQRLIQVVDRHPDLQFVLDHFGKPHLATGDIATW 178 +P ++ ++ FD VLP + + I + D PD+QF+LDH G P + W Sbjct: 121 QPLFRENVKRLSGTRFTFDLCVLPHQIPKAIALADLAPDVQFILDHCGVPDIKGHAEHPW 180 Query: 179 KDDIASLATRSNVVCKLSGLATEAAPNWQVADLSEAVDHALACFGPQRMLWGSDWPVVNL 238 +D + +A NVV K+SG+ A +W + + V+H ++ FG R++WGSDWPV L Sbjct: 181 RDHMTEIARHPNVVAKISGVVAYAEEDWALDSIRPYVEHTISVFGWDRVVWGSDWPVCTL 240 Query: 239 AGGYGHWFAAAETLLADLSSDARAAIFGGNAARIY 273 G W AA + L+ S R + GNA RI+ Sbjct: 241 GGNLSTWVAATQALIEGCSPQERRKLLSGNAQRIW 275 >AGl2610 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 297 Score = 115 bits (288), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 87/285 (30%), Positives = 128/285 (44%), Gaps = 20/285 (7%) Query: 4 IDAHHHVWTLARADYGWLTPERGPIYRDFGLADLAPHLAAAGIEGTILVQAAPTEAETAF 63 ID H H+ + Y W E + F AD A + +G+ GTI ++ + Sbjct: 21 IDTHQHLILRGKLGYAWTRGEPS-LAGSFTRADYAALVEGSGVIGTIFMETGVDDG---- 75 Query: 64 LLDIAKGAEQVRGVVG--WIDFDAADAAARIDAIAG--------REL-LVGLRPMVQDIA 112 D A V G+VG + A+ R + G R L +VG R ++Q + Sbjct: 76 --DYRAEARLVAGMVGEGTLPMFGQIASCRPETDQGFDAWLEECRHLKVVGFRRILQ-VM 132 Query: 113 DDDWLLRPELAAPLEAMARHDLVFDALVLPRHLQRLIQVVDRHPDLQFVLDHFGKPHLAT 172 DD + + + D R L ++V PD++F+LDH G P +A Sbjct: 133 PDDTSRAENYRRNVRKIGKAGWPVDLCFHSRQLAIAAELVRACPDVRFMLDHCGTPDIAG 192 Query: 173 GDIATWKDDIASLATRSNVVCKLSGLATEAAPNW-QVADLSEAVDHALACFGPQRMLWGS 231 D W + +A LA+ N+ KLSG+ AP + + V+ L FGP RM+WG Sbjct: 193 NDFPAWSERMADLASLPNLFVKLSGVTAYCAPGQVTLESVRPYVETVLKLFGPARMVWGG 252 Query: 232 DWPVVNLAGGYGHWFAAAETLLADLSSDARAAIFGGNAARIYLSS 276 DWPVV+L G W A L+ LS D +A+I GNA R YL++ Sbjct: 253 DWPVVDLGSGLPEWIAMTREFLSTLSPDEQASIGHGNARRFYLAT 297 >BMEI1718 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Bme Brucella melitensis Length = 282 Score = 113 bits (282), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 89/283 (31%), Positives = 131/283 (46%), Gaps = 18/283 (6%) Query: 4 IDAHHHVWTLARADYGWLTPERGP-IYRDFGLADLAPHLAAAGIEGT--ILVQAAP--TE 58 ID H H+ Y WL E P + RDF AGI G I V AP + Sbjct: 3 IDTHLHLIDKNALSYPWL--ESAPALNRDFLFDSYRLQAERAGIGGALHIEVDVAPHAMQ 60 Query: 59 AETAFLLDIAKGAEQ-VRGVVGWIDFDAADAAARIDAIAGRELLVGLRPMVQDIADD--D 115 AET + IA+ + ++G + + AA ++ + GLR ++ + D+ + Sbjct: 61 AETDHIRQIARRSGGFIKGAIASCRPEEPGFAAYLERQLSDPFVKGLRRVLHVVPDEVSE 120 Query: 116 WLLRPELAAPLEAMARHDLVFDALVLPRHLQRLIQVVDRHPDLQFVLDHFGKPHLATGDI 175 L E LE L FD V P + + I + D PDL F++DH G P + G + Sbjct: 121 GALFRENIKRLEGTG---LTFDLCVQPHQIGKAIALADLAPDLCFIVDHCGAPDIKAGAL 177 Query: 176 ATWKDDIASLATRSNVVCKLSGLATEA-APNWQVADLSEAVDHALACFGPQRMLWGSDWP 234 W++ +A ++ R NV+ K+SGL A +W V L + + FG R++WGSDWP Sbjct: 178 DGWREGMAEISRRPNVIAKISGLVVYCDAESWTVETLRPYAEQVIQSFGWDRVIWGSDWP 237 Query: 235 VVNLAG----GYGHWFAAAETLLADLSSDARAAIFGGNAARIY 273 V LA G W AA LL S+ ++ +F NA RI+ Sbjct: 238 VCTLASSPDAGLSTWVAATHALLEGCSTTEKSRLFCENARRIW 280