BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0097.0010.p (249 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2491 [IQR] COG1028 Dehydrogenases with different specificities... 176 5e-44 CAC3574 [IQR] COG1028 Dehydrogenases with different specificitie... 171 1e-42 SA1074 [IQR] COG1028 Dehydrogenases with different specificities... 171 1e-42 alr1894 [IQR] COG1028 Dehydrogenases with different specificitie... 166 5e-41 RSp0305 [IQR] COG1028 Dehydrogenases with different specificitie... 154 2e-37 >BH2491 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bha Bacillus halodurans Length = 246 Score = 176 bits (446), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 102/245 (41%), Positives = 142/245 (57%) Query: 4 LKGRVALVTGGGRDVGAAIAKLLAERGASVAVNYHSSKTEAEAVVAEIEKAGGKANAYQA 63 L+G+ A+VTG R +G A A LA GA+V VNY +K +AE VVAEI++ G +A A QA Sbjct: 2 LQGKTAIVTGASRGIGRATAMELARHGANVVVNYAGNKEKAEKVVAEIKELGVEAIAIQA 61 Query: 64 DISDADVVRKMVAGIKNDFGGLDILVNNAGLVFRKRFSESTPEDWKKQIDTCLYGALNCS 123 D++D++ V+ MV + FG +DILVNNAG+ F EDW IDT L G +CS Sbjct: 62 DVADSESVQAMVKETIDTFGAVDILVNNAGITRDNLFMRMKEEDWDAVIDTNLKGVFHCS 121 Query: 124 HSAGPLLEATGRGRIISIMGDSSRVGESGLALGAAARAGTIALMKSLAREWGRSGVTANS 183 + + GRII++ +G +G A AA+AG I L K+LARE +T N+ Sbjct: 122 KAVTRPMMKQRFGRIINVSSVVGAIGNAGQANYVAAKAGVIGLTKTLARELANRNITVNA 181 Query: 184 ISLGLIETAHDRTWVDANRDKLVKAYAIRRLGLPSDVAPLVALLASDAGSWITGQVISVN 243 ++ G IET + + +++ + RLG P +VA V LASD S++TGQ I VN Sbjct: 182 VAPGFIETDMTGELPEDVKAQMLGQIPLARLGQPEEVAKAVRFLASDDASYLTGQTIHVN 241 Query: 244 GGYSM 248 GG M Sbjct: 242 GGMVM 246 >CAC3574 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Cac Clostridium acetobutylicum Length = 249 Score = 171 bits (433), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 98/245 (40%), Positives = 143/245 (58%) Query: 4 LKGRVALVTGGGRDVGAAIAKLLAERGASVAVNYHSSKTEAEAVVAEIEKAGGKANAYQA 63 L G+VA+VTG GR +G AIA LA GA++ VNY SS+ E + ++ EIE+ G KA A +A Sbjct: 5 LSGKVAVVTGAGRGLGRAIALKLAAEGANLVVNYRSSEAETQKLIKEIEELGSKAVAVKA 64 Query: 64 DISDADVVRKMVAGIKNDFGGLDILVNNAGLVFRKRFSESTPEDWKKQIDTCLYGALNCS 123 DIS D ++ +++G +DILVNNAG+ ED+ I+ L GA NC Sbjct: 65 DISKYDEAETIIKKALDEYGTVDILVNNAGITKDNLLLRMKEEDFDSVINVNLKGAFNCI 124 Query: 124 HSAGPLLEATGRGRIISIMGDSSRVGESGLALGAAARAGTIALMKSLAREWGRSGVTANS 183 ++ G+II+I +G +G AAA+AG I + KS+A+E G+T N+ Sbjct: 125 KHTSRVMLKKKSGKIINISSVIGLIGNAGQVNYAAAKAGIIGMTKSVAKELASRGITVNA 184 Query: 184 ISLGLIETAHDRTWVDANRDKLVKAYAIRRLGLPSDVAPLVALLASDAGSWITGQVISVN 243 ++ G+I++ D R+ +V A + ++G DVA LV LASD S+ITGQVI+V+ Sbjct: 185 VAPGIIKSDMTDALTDKQRESIVAAVPLNKVGEAEDVANLVLFLASDLSSYITGQVINVD 244 Query: 244 GGYSM 248 GG M Sbjct: 245 GGMVM 249 >SA1074 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sau Staphylococcus aureus N315 Length = 246 Score = 171 bits (433), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 99/242 (40%), Positives = 135/242 (55%) Query: 7 RVALVTGGGRDVGAAIAKLLAERGASVAVNYHSSKTEAEAVVAEIEKAGGKANAYQADIS 66 + ALVTG R +G +IA LAE G +VAVNY SK +AEAVV EI+ G + A QA+++ Sbjct: 5 KSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVVEEIKAKGVDSFAIQANVA 64 Query: 67 DADVVRKMVAGIKNDFGGLDILVNNAGLVFRKRFSESTPEDWKKQIDTCLYGALNCSHSA 126 DAD V+ M+ + + FG LD+LVNNAG+ ++W IDT L G NC A Sbjct: 65 DADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQEWDDVIDTNLKGVFNCIQKA 124 Query: 127 GPLLEATGRGRIISIMGDSSRVGESGLALGAAARAGTIALMKSLAREWGRSGVTANSISL 186 P + G II++ VG G A A +AG I L KS ARE G+T N+++ Sbjct: 125 TPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVIGLTKSAARELASRGITVNAVAP 184 Query: 187 GLIETAHDRTWVDANRDKLVKAYAIRRLGLPSDVAPLVALLASDAGSWITGQVISVNGGY 246 G I + D +++++ + R G +D+A VA LASD +ITGQ I VNGG Sbjct: 185 GFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIANTVAFLASDKAKYITGQTIHVNGGM 244 Query: 247 SM 248 M Sbjct: 245 YM 246 >alr1894 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Nos Nostoc sp. PCC 7120 Length = 251 Score = 166 bits (420), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 100/247 (40%), Positives = 147/247 (59%), Gaps = 4/247 (1%) Query: 3 DLKGRVALVTGGGRDVGAAIAKLLAERGASVAVNYHSSKTEAEAVVAEIEKAGGKANAYQ 62 +L+G+VA+VTG R +G AIA LA GA+V VNY SS T A+ VVAEI AGG+A A + Sbjct: 7 NLRGQVAVVTGASRGIGRAIALELANYGATVVVNYASSSTAADEVVAEITGAGGEAVALK 66 Query: 63 ADISDADVVRKMVAGIKNDFGGLDILVNNAGLVFRKRFSESTPEDWKKQIDTCLYGALNC 122 AD+S + V ++ G + F +DILVNNAG+ PEDW+ ID L G C Sbjct: 67 ADVSQVEQVDNLINGAIDKFKRIDILVNNAGITRDTLLLRMKPEDWQAVIDLNLTGVFLC 126 Query: 123 SHSAGPLLEATGRGRIISIMGDSSRVGESGLALGAAARAGTIALMKSLAREWGRSGVTAN 182 + + L+ GRII+I + ++G G A +AA+AG I K++A+E G+T N Sbjct: 127 TRAVSKLMLKQRSGRIINITSVAGQMGNPGQANYSAAKAGVIGFTKTVAKELASRGITVN 186 Query: 183 SISLGLIETAHDRTWVDANRDKLVKAYAIRRLGLPSDVAPLVALLASD-AGSWITGQVIS 241 +++ G I T D T + + +++ + R G P ++A +V LA+D A ++ITGQV + Sbjct: 187 AVAPGFIAT--DMTS-NLKSEGILQYIPLGRYGQPEEIAGMVRFLAADPAAAYITGQVFN 243 Query: 242 VNGGYSM 248 V+GG M Sbjct: 244 VDGGMVM 250 >RSp0305 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Rso Ralstonia solanacearum Length = 255 Score = 154 bits (389), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 96/243 (39%), Positives = 135/243 (55%), Gaps = 1/243 (0%) Query: 4 LKGRVALVTGGGRDVGAAIAKLLAERGASVAVNYHSSKTEAEAVVAEIEKAGGKANAYQA 63 L GR A+VTGG R +GAAIA+ LA GA VAV Y S EA+AVV I G KA A QA Sbjct: 13 LAGRAAIVTGGSRGIGAAIARRLAADGAHVAVVYRSHHDEADAVVRAIRDTGAKAIAVQA 72 Query: 64 DISDADVVRKMVAGIKNDFGGLDILVNNAGLVFRKRFSESTPEDWKKQIDTCLYGALNCS 123 D+SDA VR MV ++ FG +DILVNNAG++ + + +Q + A+ S Sbjct: 73 DVSDAASVRAMVDTVQRAFGEIDILVNNAGILASQPVGSIDQAAFDRQFRINAFSAILVS 132 Query: 124 HSAGPLLEATGRGRIISIMGDSSRVGESGLALGAAARAGTIALMKSLAREWGRSGVTANS 183 + P + A G GRI+++ +GLA+ AA++A AL ++ A E G +T N+ Sbjct: 133 QAVLPQMPARG-GRIVNVSSSLVFRPRAGLAVYAASKAAVSALTQAFALELGPRNITVNA 191 Query: 184 ISLGLIETAHDRTWVDANRDKLVKAYAIRRLGLPSDVAPLVALLASDAGSWITGQVISVN 243 ++ + T DA + +L A + RL P D+A VA LA D WITG+ + + Sbjct: 192 VAPAMTRTDMTAPLPDALKARLRDATPLGRLAEPEDIADAVAFLACDDSRWITGRTLLTD 251 Query: 244 GGY 246 GG+ Sbjct: 252 GGF 254