BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0087.0037.p (642 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr7070 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 582 e-166 CC1830 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 447 e-125 YBR208c_1 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A... 406 e-113 CAC2977 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 164 8e-40 BMEII0197 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A... 152 2e-36 >mlr7070 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Mlo Mesorhizobium loti Length = 619 Score = 582 bits (1500), Expect = e-166, Method: Compositional matrix adjust. Identities = 324/633 (51%), Positives = 401/633 (63%), Gaps = 43/633 (6%) Query: 13 NDVTETVAAIVAAHRAGTMTPARTVARSYQRIREHNDPAIFISLRDEKDALAEAGRLA-- 70 +DV +A++ AA+R G +A Y RI +DP IFI L + + L A L Sbjct: 2 SDVHFDIASLHAAYRDGVGV-GDVIATIYARIEAADDPGIFIHLVSKANLLTAAEALGPF 60 Query: 71 DPTL-PLYGVPVAVKDNIDVKGLPTTAACPAFSYLPAQDSTAVARLRAAGAIIIGKTNLD 129 DP PL+G+P AVKDNIDV G+PTTAAC ++Y P +D+T VARL+AAGA+++GKTNLD Sbjct: 61 DPAAKPLWGIPFAVKDNIDVAGMPTTAACAEYTYWPDEDATVVARLKAAGALVVGKTNLD 120 Query: 130 QFATGLVGVRSPYGIPKNPVRGDLVPGGSSSGSAVAVSAGLVPLALGTDTAGSGRVPAML 189 QFATGLVGVR+PY IP+N + +LVPGGSSSGSAVA + G+V ALGTDTAGSGR+PA L Sbjct: 121 QFATGLVGVRTPYPIPRNAIDAELVPGGSSSGSAVATARGIVSFALGTDTAGSGRIPAGL 180 Query: 190 NNIVGLKPSLGLISTTGVVPACRTLDCVSIFSLTVDDALAALAVMSGPDQSDPFSRGRPL 249 NNIVGLKP++G +ST GVVPACRTLDCVS+F+LTVDDA A +V + D +DP+SR + Sbjct: 181 NNIVGLKPTVGALSTGGVVPACRTLDCVSVFALTVDDAYAVFSVAAARDAADPYSRDIAV 240 Query: 250 APLAAFPAKLKLGIPQSGQLKFFSDHEAEKAYGEACKRWQALGADLVGFDLAPFYETARL 309 PLAA P L +GIP LKFF D + + + LG LV FY TA L Sbjct: 241 QPLAARPPVLSVGIPAKADLKFFGDVAMQAGFEASLAMLSRLGCRLVEIPFGDFYATANL 300 Query: 310 LYEGPWVAERYLVIRDLLASSPEAVHPVTREITVGGSRPTAADTFSALYRLEALRRVAER 369 LYEG WVAERY IRD + ++ A+HPVTR+I G + +AAD F LY L+A R Sbjct: 301 LYEGAWVAERYAAIRDFMEANEAAMHPVTRKIIGGARKLSAADAFRGLYALQAYRAKLAA 360 Query: 370 SLAHLDAIVLPTAPTVYSTADVLANPIELNSRLGTYTNFVNLLDMCGLALPAAMRPDGAP 429 +A +D +PTAPT Y+ A VLA+P+E NSRLGTYTNFVNLLDMCG+A+P R DG P Sbjct: 361 VIASVDLFCVPTAPTHYTAAAVLADPVETNSRLGTYTNFVNLLDMCGIAVPTGKRDDGLP 420 Query: 430 FGITLLAPAGRDAELAGIGRVFHADTGLALGANSLPQP---PLAAAPMQTGSGEITIAVV 486 +TLLAPAGRD A + R H +GL+LGA PQP P+AA P G I + VV Sbjct: 421 MSVTLLAPAGRDVLTAMLARDLHVQSGLSLGATGWPQPNLQPVAAPPCD---GMIDLVVV 477 Query: 487 GAHLSGMALNHELTTLDARLLVGAALGLDLTGMITGAKRTLTKDEADHLERLAQXEAVTA 546 GAHLSGM LN +L L A+ L + T T Q Sbjct: 478 GAHLSGMPLNGQLNQLGAQFL----------------RATKTAAAYRLYALAGQ------ 515 Query: 547 PDYKLYALDTTPPKPGMLRIEAGAGASIKLELWAMSPAAFGTFVAAIPPPLSIGTIRLAD 606 + PKPG++R+ G G I +E+W + P AFG FVAAIP PL IGT+ L D Sbjct: 516 ----------SVPKPGLIRVADG-GTRIDVEIWRLEPEAFGRFVAAIPAPLGIGTVELDD 564 Query: 607 GRQAKGFIVEPAAIHGASDISSYGGWRAFMAER 639 G AKGF+VE A + GASDISS GGWR F+A Sbjct: 565 GTPAKGFLVETAGLDGASDISSLGGWRRFVARN 597 >CC1830 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Ccr Caulobacter vibrioides Length = 594 Score = 447 bits (1150), Expect = e-125, Method: Compositional matrix adjust. Identities = 293/613 (47%), Positives = 370/613 (60%), Gaps = 52/613 (8%) Query: 34 ARTVARSYQRIREHND---PAIFISLRDEKDALAEAGRL-----ADPTLPLYGVPVAVKD 85 A +VARS E D P ++ E LA+A + A TLPL GVP AVKD Sbjct: 21 AESVARSALAAIEAYDAIQPQVWTLRLPEAAVLAQARAVDARIAAGETLPLAGVPFAVKD 80 Query: 86 NIDVKGLPTTAACPAFSYLPAQDSTAVARLRAAGAIIIGKTNLDQFATGLVGVRSPYGIP 145 NIDV L TTAACP F+Y + +T V +L AAGA+++GKTNLDQFATGLVG RSPYG P Sbjct: 81 NIDVADLATTAACPGFAYTAGRSATVVEQLLAAGAVLVGKTNLDQFATGLVGTRSPYGAP 140 Query: 146 KNPVRGDLVPGGSSSGSAVAVSAGLVPLALGTDTAGSGRVPAMLNNIVGLKPSLGLISTT 205 + V GGSSSGSAVAV+AGLV ALGTDTAGSGRVPA N++VGLKPS G ST Sbjct: 141 RCVFNQAFVSGGSSSGSAVAVAAGLVAFALGTDTAGSGRVPAAFNHLVGLKPSKGRWSTR 200 Query: 206 GVVPACRTLDCVSIFSLTVDDALAALAVMSGPDQSDPFSRGRPLAPLAAFPAKLKLGIPQ 265 G+VPACR+LDC+S+F+ +D A AV++ D DP+SR P A A L++ IP+ Sbjct: 201 GLVPACRSLDCISVFAADLDGAGRVDAVLAAFDPEDPYSRPAPQPEPALTLAGLRVAIPR 260 Query: 266 SGQLKFFSDHEAEKAYGEACKRWQALGADLVGFDLAPFYETARLLYEGPWVAERYLVIRD 325 Q FF D E+E Y A R QA GA V D+AP + A+LLY GPWVAER + Sbjct: 261 PEQRIFFGDGESEALYASAVARLQAAGAVAVEVDMAPLLDAAKLLYSGPWVAERTAAVEP 320 Query: 326 LLASSPEAVHPVTREITVGGSRPTAADTFSALYRLEALRRVAERSLAHLDAIVLPTAPTV 385 LL ++P A+ P R I GG T +TF LY LEA RR AE A DA+ LPT PT+ Sbjct: 321 LLRAAPSAIEPTVRAIVQGGLAVTGVETFRGLYALEAYRRAAEAVWAAADALFLPTTPTI 380 Query: 386 YSTADVLANPIELNSRLGTYTNFVNLLDMCGLALPAAMRPDGAPFGITLLAPAGRDAELA 445 Y+ + A P+ LN+ LG YTNFVNLLD+ LA+PA R DG FG+T + PA D L Sbjct: 381 YTVKALKAEPLALNANLGLYTNFVNLLDLSALAVPAGFRADGTGFGVTFIGPAFADRALL 440 Query: 446 GIGRVFHADTGLALGANSLPQPPLAAAPMQTGSGEITIAVVGAHLSGMALNHELTTLDAR 505 + V+ +A PPL P + G + +AVVGAHLSGM L+ +LT+ +AR Sbjct: 441 DLAAVYLETFPMA------ETPPLDLTPHKPG---VKLAVVGAHLSGMPLHWQLTSREAR 491 Query: 506 LLVGAALGLDLTGMITGAKRTLTKDEADHLERLAQXEAVTAPDYKLYAL-DTTPPKPGML 564 L+ A RT AP YKLYA+ ++TPPKP ++ Sbjct: 492 LV--------------AATRT-------------------APAYKLYAMAESTPPKPALV 518 Query: 565 RIEAGAGASIKLELWAMSPAAFGTFVAAIPPPLSIGTIRLADGRQAKGFIVEPAAIHGAS 624 + G GASI +E++ + AAFG F A +P PL+IGT+ L DG KGF+ EP A++GAS Sbjct: 519 HVGDG-GASILVEVYELDVAAFGAFTAEVPAPLAIGTVTLEDGTSVKGFVAEPRALNGAS 577 Query: 625 DISSYGGWRAFMA 637 DI+ GGWRA++A Sbjct: 578 DITVLGGWRAYIA 590 >YBR208c_1 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Sce Saccharomyces cerevisiae Length = 631 Score = 406 bits (1043), Expect = e-113, Method: Compositional matrix adjust. Identities = 242/603 (40%), Positives = 336/603 (55%), Gaps = 54/603 (8%) Query: 53 FISLRDEKDALAE----AGRLADPTLPLYGVPVAVKDNIDVKGLPTTAACPAFSYLPAQD 108 +ISL +++ L + R TLPLYGVP+AVKDNIDV+GLPTTAACP+F+Y P++D Sbjct: 52 WISLISKENLLHQFQILKSRENKETLPLYGVPIAVKDNIDVRGLPTTAACPSFAYEPSKD 111 Query: 109 STAVARLRAAGAIIIGKTNLDQFATGLVGVRSPYGIPKNPVRGDLVPGGSSSGSAVAVSA 168 S V LR AGAII+GKTNLDQFATGLVG RSPYG + V GGSS+GSA V+ Sbjct: 112 SKVVELLRNAGAIIVGKTNLDQFATGLVGTRSPYGKTPCAFSKEHVSGGSSAGSASVVAR 171 Query: 169 GLVPLALGTDTAGSGRVPAMLNNIVGLKPSLGLISTTGVVPACRTLDCVSIFSLTVDDAL 228 G+VP+ALGTDTAGSGRVPA LNN++GLKP+ G+ S GVVPAC++LDCVSIF+L + DA Sbjct: 172 GIVPIALGTDTAGSGRVPAALNNLIGLKPTKGVFSCQGVVPACKSLDCVSIFALNLSDAE 231 Query: 229 AALAVMSGPD-QSDPFSRGRPLAPLAAFPAKLKLGIPQSGQLKFFSDHEAEKAYGEACKR 287 +M PD +D +SR PL F + + + IP++ + ++ + + + A + Sbjct: 232 RCFRIMCQPDPDNDEYSRPYVSNPLKKFSSNVTIAIPKN--IPWYGETKNPVLFSNAVEN 289 Query: 288 WQALGADLVGFDLAPFYETARLLYEGPWVAERYLVIRDLLASSP--EAVHPVTREITVGG 345 GA+++ D P E AR LYEG WVAERY I+ L S P E++ P I G Sbjct: 290 LSRTGANVIEIDFEPLLELARCLYEGTWVAERYQAIQSFLDSKPPKESLDPTVISIIEGA 349 Query: 346 SRPTAADTFSALYRLEALRRVAERSLAHLDAIVLPTAPTVYSTADVLANPIELNSRLGTY 405 + +A D FS Y+ + + + R L +D + +PT P + V P+ +NSR GT+ Sbjct: 350 KKYSAVDCFSFEYKRQGILQKVRRLLESVDVLCVPTCPLNPTMQQVADEPVLVNSRQGTW 409 Query: 406 TNFVNLLDMCGLALPAAMRPDGAPFGITLLAPAGRDAELAGIGRVFHADT--------GL 457 TNFVNL D+ LA+PA R DG P GITL+ D L + + + G Sbjct: 410 TNFVNLADLAALAVPAGFRDDGLPNGITLIGKKFTDYALLELANRYFQNIFPNGSRTYGT 469 Query: 458 ALGANSLPQPPLAAAPMQTGSGEITIAVVGAHLSGMALNHELTTLDARLLVGAALGLDLT 517 ++ P P S I +AVVGAHL G+ L+ +L ++A L Sbjct: 470 FTSSSVKPANDQLVGPDYDPSTSIKLAVVGAHLKGLPLHWQLEKVNATYLCTTK------ 523 Query: 518 GMITGAKRTLTKDEADHLERLAQXEAVTAPDYKLYALDTTPP--KPGMLRIEAGAGASIK 575 T+ Y+L+AL P KPG+ R++ G+ I+ Sbjct: 524 ---------------------------TSKAYQLFALPKNGPVLKPGLRRVQDSNGSQIE 556 Query: 576 LELWAMSPAAFGTFVAAIPPPLSIGTIRLADGRQAKGFIVEPAAI--HGASDISSYGGWR 633 LE++++ FG F++ +P PL IG++ L G K FI E + G DI+ YGG+R Sbjct: 557 LEVYSVPKELFGAFISMVPEPLGIGSVELESGEWIKSFICEESGYKAKGTVDITKYGGFR 616 Query: 634 AFM 636 A+ Sbjct: 617 AYF 619 >CAC2977 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Cac Clostridium acetobutylicum Length = 478 Score = 164 bits (414), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 132/446 (29%), Positives = 219/446 (49%), Gaps = 49/446 (10%) Query: 34 ARTVARSYQRIREHNDPAI--FISLRDEKDALAEAGRL------ADPTLPLYGVPVAVKD 85 + + ++Y + N+P I +I+L ++ AL EA + D L G+P+A+KD Sbjct: 22 SEDIVKAYFDNIKRNEPEINAYITLCEDY-ALKEAKDVDKKIANGDKVGRLAGIPIAIKD 80 Query: 86 NIDVKGLPTTAACPA-FSYLPAQDSTAVARLRAAGAIIIGKTNLDQFATGLVGVRSPYGI 144 NI G+ TT A + ++P D+T + +L+A AIIIGK N+D+FA G S + I Sbjct: 81 NICTDGIKTTCASKMLYDFVPPYDATVIKKLKAEDAIIIGKVNMDEFAMGSSTENSAFKI 140 Query: 145 PKNPVRGDLVPGGSSSGSAVAVSAGLVPLALGTDTAGSGRVPAMLNNIVGLKPSLGLIST 204 KNP VPGGSS GSA V+A + P++LG+DT GS R PA +VGLKP+ GL+S Sbjct: 141 TKNPRDITRVPGGSSGGSAAVVAAKMAPISLGSDTGGSIRQPAAFCGVVGLKPTYGLVSR 200 Query: 205 TGVVPACRTLDCVSIFSLTVDDALAALAVMSGPDQSDPFSRGRP-----LAPLAAFPAKL 259 G++ +LD + TV D L V+SG D+ D S + L + + Sbjct: 201 FGLIAFASSLDQIGPLGKTVKDCAELLEVISGEDELDNTSSKKHEKEDYLEGIDDGIKGM 260 Query: 260 KLGIPQSGQLKFFSDH---EAEKAYGEACKRWQALGADLVGFDLAPFYETARLLYEGPWV 316 K+G+P+ +F +D E K + ++ ++LGA++ L P E Y Sbjct: 261 KIGMPK----EFLNDGLDPEIRKCIDDTIEKLKSLGAEVCEMSL-PITEEGLSAYYIISS 315 Query: 317 AE------RYLVIR------------DLLASS-PEAVHPVTREITVGGSRPTAADTFSAL 357 AE R+ IR DL+ +S EA + + G+ ++ + A Sbjct: 316 AEASSNLARFDGIRYGYRPDDFEDVYDLMETSRSEAFGDEVKRRIMLGTYALSSGYYDAY 375 Query: 358 YR-----LEALRRVAERSLAHLDAIVLPTAPTV-YSTADVLANPIELNSRLGTYTNFVNL 411 Y+ + ++ + + + D I+ P +P + + + A+P+++ YT +NL Sbjct: 376 YKRALKLKKKIKNEFKEAFENYDLILSPVSPVLPFKIGEKKADPLQM-YLADIYTVNINL 434 Query: 412 LDMCGLALPAAMRPDGAPFGITLLAP 437 + ++LP ++ +G P G+ LL P Sbjct: 435 AGIPAISLPCSVSKEGLPIGLQLLGP 460 >BMEII0197 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Bme Brucella melitensis Length = 472 Score = 152 bits (385), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 145/483 (30%), Positives = 225/483 (46%), Gaps = 36/483 (7%) Query: 14 DVTETVAA-IVAAHRAGTMTPARTVARSYQRIREHNDPA-IFISLRDEKDALAEAGRLAD 71 D+TE AA +V +P+ +A RI HN +++R + +AEA L D Sbjct: 2 DLTELSAARLVNMMGKRQTSPSEVMAAHLDRIERHNGAVNAMVAMRSRDELIAEARLLDD 61 Query: 72 PTL--PLYGVPVAVKDNIDVKGLPTTAACPAFS-YLPAQDSTAVARLRAAGAIIIGKTNL 128 + PL+G+P A+KD + K + +T P + Y+PA+D+ AV+R+R AGA+I+GKTN Sbjct: 62 KPVCGPLHGLPWAIKDLLPTKDIRSTWGSPIHADYIPAEDALAVSRIRKAGAVIMGKTNT 121 Query: 129 DQFATGLVGVRSPYGIPKNPVRGDLVPGGSSSGSAVAVSAGLVPLALGTDTAGSGRVPAM 188 ++ G YG+ NP L GGSS G+A A++A +PL G+D GS R PA Sbjct: 122 PEWGHGSHSFNPVYGVTCNPYDTQLSAGGSSGGTAAALAAHFMPLGDGSDMMGSLRNPAG 181 Query: 189 LNNIVGLKPSLGLI-STTGVVPACRTLDCVSIFSLTVDDALAALAVMSGPDQSDPFSRGR 247 NI G +PS GL+ + G T+ + + ++D + L V++ P PF + Sbjct: 182 FCNIYGYRPSWGLVPNEIGGDLFVHTMATLGPMARDIEDLVLLLDVLAEPYSLSPFGQKH 241 Query: 248 PLAPLAAFPAKLKLGIPQSGQLKFFSDHEAEKAYGEACKRWQA----LGADLVGFDLAPF 303 PA L+ G + + + E E C++ A LGA++V + PF Sbjct: 242 TGFATNHAPADLR-GKRIAWLGNWGGAYPCEAGILERCEKGLAVLSELGAEIVPLE-PPF 299 Query: 304 YETARLLYEGPWVAERYLVI---RDLLASSPEA---VHPVTREITVGGSRPTAADTFSAL 357 +++ L+E W+ R LVI + L PE + P T G+ TA A Sbjct: 300 --SSQALWEA-WITLRSLVIYGSKRALWEKPETRKLIKPETLWEIENGAGLTAQQIMHAS 356 Query: 358 YRLEALRRVAERSLAHLDAIVLPTAPTVYSTAD------VLANPIELNSRLGTYTNFVNL 411 A R A DA+++PTA AD + P++ R V+L Sbjct: 357 QIRSDWYVSAHRLFAEYDALIMPTAQVWPFPADWRWPQHINGQPMDSYHRWMEVVVPVSL 416 Query: 412 LDMCGLALPAAMRPDGAPFGITLLAPAGRDAELAGIGRVFHADTGLALGANSLPQ--PPL 469 + + L++P G P G+ ++ +G DAE+ IG +H A PQ PP+ Sbjct: 417 IGLAALSVPVGFDQRGRPTGMQIIGASGADAEILAIGETYHR-------ATLWPQRKPPM 469 Query: 470 AAA 472 AA Sbjct: 470 LAA 472