BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0086.0001.p (390 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1446 [R] COG3550 Uncharacterized protein related to capsule b... 292 8e-79 hipA [R] COG3550 Uncharacterized protein related to capsule bios... 285 9e-77 ZhipA [R] COG3550 Uncharacterized protein related to capsule bio... 242 1e-63 ECs2115 [R] COG3550 Uncharacterized protein related to capsule b... 129 9e-30 CC2386 Non COG 129 1e-29 >RSc1446 [R] COG3550 Uncharacterized protein related to capsule biosynthesis enzymes Rso Ralstonia solanacearum Length = 445 Score = 292 bits (748), Expect = 8e-79, Method: Compositional matrix adjust. Identities = 169/390 (43%), Positives = 232/390 (59%), Gaps = 19/390 (4%) Query: 8 GTAVTNVFDNLLPDSEPIRKRVAERVGAAGTDAYSMLSALGHDCVGALQFLPEGIEAGRA 67 G V + FDNLLP+++PIR+RV R G A TDA +L+A+G DCVGA+Q LP G+ Sbjct: 58 GQVVDDYFDNLLPEAQPIRERVRRRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGY 117 Query: 68 GAIDGKHISNDGIAEIIANLATAPLGLGED----DAFRISIAGAQEKTALLRREGKWYTP 123 ID + +++ + ++ NL P LGED DAFRISIAGAQEKTALLR W+ P Sbjct: 118 DRIDSEALTDSQVDTLLRNLTAMP-ALGEDAGDPDAFRISIAGAQEKTALLRIGDAWHRP 176 Query: 124 AGTTATTHILKPQIGRLPN-GGDLSNSVENEYFCLKLLARLGIPTAHAEMADFGGRRTLV 182 G T TTHILK +G + N D+ +SV+NE+ C +LLA G+ A E+A FG + L Sbjct: 177 LGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLA 236 Query: 183 VQRFDRLWT-ADKRLLRLPQEDCCQALSIAPTRKYQSEGGPGLKEILELLKGSDVPGEDL 241 V+RFDR W + + RLPQED CQA RKY+++GGPG++ L+LL S+ +D Sbjct: 237 VERFDRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDK 296 Query: 242 ATFMRAAIVFWLIGATDGHAKNFSIFLTPGGRFQLTPLYDVLSAQPSL--DARQIQPKAF 299 F+ A + FWL+ ATDGHAKNFS+ + GGRF++TPLYDVLS P + Q+ P Sbjct: 297 RRFVLAQLAFWLLAATDGHAKNFSLAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRA 356 Query: 300 KLAMSV-GKSRHYTVNEILPRHFLQTADLSKVISQASVRTLFID---LVSRFEEAFDEVT 355 +LAM++ GK+ H + +I RH+ A +Q+ V F LV R + V Sbjct: 357 RLAMALRGKNAHARLADIHVRHWQALA------AQSGVPDAFAGMQALVERVPAGLERVE 410 Query: 356 KGLPKSFPKDLTTSIRAAALHRIELIKDEL 385 LP F + +S+R L + E +EL Sbjct: 411 AILPPDFSGRVWSSVRTGVLAQAERFLNEL 440 >hipA [R] COG3550 Uncharacterized protein related to capsule biosynthesis enzymes Eco Escherichia coli K12 Length = 440 Score = 285 bits (730), Expect = 9e-77, Method: Compositional matrix adjust. Identities = 160/374 (42%), Positives = 218/374 (58%), Gaps = 10/374 (2%) Query: 10 AVTNVFDNLLPDSEPIRKRVAERVGAAGTDAYSMLSALGHDCVGALQFLPEGIEAGRAGA 69 AV N FDNLLPDS +R R+ +R A + +LS +G D VGA+ +PE E Sbjct: 56 AVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVTLIPED-ETVTHPI 114 Query: 70 IDGKHISNDGIAEII-ANLATAPLGL-GEDDAFRISIAGAQEKTALLRREGKWYTPAGTT 127 + + ++ + E++ A A PLG+ E++ FRIS+AGAQEKTALLR W P G T Sbjct: 115 MAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTALLRIGNDWCIPKGIT 174 Query: 128 ATTHILKPQIG--RLPNGG-DLSNSVENEYFCLKLLARLGIPTAHAEMADFGGRRTLVVQ 184 TTHI+K IG R PN DLS SV+NEY+CL L LG+ AE+ G R L V+ Sbjct: 175 PTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVE 234 Query: 185 RFDRLWTADKR-LLRLPQEDCCQALSIAPTRKYQSEGGPGLKEILELLKGSDVPGEDLAT 243 RFDR W A++ LLRLPQED CQ + + KY+S+GGPG+ I+ L GS +D Sbjct: 235 RFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYD 294 Query: 244 FMRAAIVFWLIGATDGHAKNFSIFLTPGGRFQLTPLYDVLSAQPSLDARQIQPKAFKLAM 303 FM+ + WLIGATDGHAKNFS+F+ GG ++LTP YD++SA P L I KLAM Sbjct: 295 FMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAM 354 Query: 304 --SVGKSRHYTVNEILPRHFLQTADLSKVISQASVRTLFIDLVSRFEEAFDEVTKGLPKS 361 + K + +++I PRHFL TA + + + + + D A D V LP Sbjct: 355 GLNASKGKKTAIDKIYPRHFLATAKVLR-FPEVQMHEILSDFARMIPAALDNVKTSLPTD 413 Query: 362 FPKDLTTSIRAAAL 375 FP+++ T++ + L Sbjct: 414 FPENVVTAVESNVL 427 >ZhipA [R] COG3550 Uncharacterized protein related to capsule biosynthesis enzymes EcZ Escherichia coli O157:H7 EDL933 Length = 340 Score = 242 bits (618), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 132/295 (44%), Positives = 173/295 (58%), Gaps = 8/295 (2%) Query: 88 ATAPLGL-GEDDAFRISIAGAQEKTALLRREGKWYTPAGTTATTHILKPQIG--RLPNGG 144 A PLG+ E++ FRIS+AGAQEKTALLR W P G T TTHI+K IG R PN Sbjct: 34 ADIPLGMIREENDFRISVAGAQEKTALLRIGNDWCIPKGITPTTHIIKLPIGEIRQPNAT 93 Query: 145 -DLSNSVENEYFCLKLLARLGIPTAHAEMADFGGRRTLVVQRFDRLWTADKR-LLRLPQE 202 DLS SV+NEY+CL L LG+ AE+ G R L V+RFDR W A++ LLRLPQE Sbjct: 94 LDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQE 153 Query: 203 DCCQALSIAPTRKYQSEGGPGLKEILELLKGSDVPGEDLATFMRAAIVFWLIGATDGHAK 262 D CQ + + KY+S+GGPG+ I+ L G D FM+ + WLIGATDGHAK Sbjct: 154 DMCQTFGLPSSVKYESDGGPGIARIMAFLMGFSEALRDRYDFMKFQVFQWLIGATDGHAK 213 Query: 263 NFSIFLTPGGRFQLTPLYDVLSAQPSLDARQIQPKAFKLAMSVG--KSRHYTVNEILPRH 320 NFS+F+ GG ++LTP YD++SA P L I KLAM + K + +++I PRH Sbjct: 214 NFSVFIQAGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRH 273 Query: 321 FLQTADLSKVISQASVRTLFIDLVSRFEEAFDEVTKGLPKSFPKDLTTSIRAAAL 375 FL TA + + + + + D A D V LP FP+++ T++ L Sbjct: 274 FLATAKVLR-FPEVQMHEILSDFARMIPAALDNVKTSLPTDFPENVVTAVETNVL 327 >ECs2115 [R] COG3550 Uncharacterized protein related to capsule biosynthesis enzymes Ecs Escherichia coli O157:H7 Length = 186 Score = 129 bits (325), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 65/173 (37%), Positives = 94/173 (54%), Gaps = 3/173 (1%) Query: 205 CQALSIAPTRKYQSEGGPGLKEILELLKGSDVPGEDLATFMRAAIVFWLIGATDGHAKNF 264 CQ + + KY+S+GGPG+ I+ L G D FM+ + WLIGATDGHAKNF Sbjct: 2 CQTFGLPSSVKYESDGGPGIARIMAFLMGFSEALRDRYDFMKFQVFQWLIGATDGHAKNF 61 Query: 265 SIFLTPGGRFQLTPLYDVLSAQPSLDARQIQPKAFKLAMSVG--KSRHYTVNEILPRHFL 322 S+F+ GG ++LTP YD++SA P L I KLAM + K + +++I PRHFL Sbjct: 62 SVFIQAGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFL 121 Query: 323 QTADLSKVISQASVRTLFIDLVSRFEEAFDEVTKGLPKSFPKDLTTSIRAAAL 375 TA + + + + + D A D V LP FP+++ T++ L Sbjct: 122 ATAKVLR-FPEVQMHEILSDFARMIPAALDNVKTSLPTDFPENVVTAVETNVL 173 >CC2386 Non COG Length = 198 Score = 129 bits (323), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 63/131 (48%), Positives = 84/131 (64%), Gaps = 1/131 (0%) Query: 252 WLIGATDGHAKNFSIFLTPGGRFQLTPLYDVLSAQPSLDARQIQPKAFKLAMSVGKSRHY 311 WL+GATDGHAKNFS+ L PGGRF+L PLYD+LS QP +DA Q++ +KL+M+VG +RHY Sbjct: 67 WLLGATDGHAKNFSVALDPGGRFRLKPLYDILSVQPLVDAGQVRHNQYKLSMAVGHNRHY 126 Query: 312 TVNEILPRHFLQTADLSKVISQASVRTLFIDLVSRFEEAFDEVTKGLPKSFPKDLTTSIR 371 I PRHF+QTA ++ I Q V + L R A + V GLP FP L S Sbjct: 127 AAGSIRPRHFIQTARRAE-IGQRVVEAVLEALGQRTPAALEAVEAGLPAEFPVGLVNSTL 185 Query: 372 AAALHRIELIK 382 A R+++++ Sbjct: 186 AGVHARLQIVR 196