BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0082.0021.p (213 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0255 [S] COG3619 Predicted membrane protein Pae Pseudomonas ae... 91 2e-18 AGc254 [S] COG3619 Predicted membrane protein Atu Agrobacterium ... 83 5e-16 BMEI0217 [S] COG3619 Predicted membrane protein Bme Brucella mel... 82 6e-16 SMa1297 [S] COG3619 Predicted membrane protein Sme Sinorhizobium... 77 3e-14 SMa1162 [S] COG3619 Predicted membrane protein Sme Sinorhizobium... 73 4e-13 >PA0255 [S] COG3619 Predicted membrane protein Pae Pseudomonas aeruginosa Length = 228 Score = 90.9 bits (224), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 68/176 (38%), Positives = 98/176 (55%), Gaps = 7/176 (3%) Query: 8 LALACALSALAGYVDGIGYLHLGGLFVSFMSGNSTRLGVTLAEGHWQHALEALALIVLFV 67 L L LS LAG D IG+L G FVSFMSGN+TRL V+L EG + + + FV Sbjct: 20 LGLVAGLSVLAGMTDAIGFLATGD-FVSFMSGNTTRLAVSLGEGDFGGLVRLSVALASFV 78 Query: 68 AGAAAGSLIVLSALAHRQ--PLLLLFEAWLLTAAAVAYAYGLPSAAVAAIVLAMGLENAV 125 G A G ++L+ + R+ P+L+ L +A + + +P A+ A +LAMG+ NAV Sbjct: 79 VGNALG--VILARFSGRRAAPILVCCAMLLALSAVWPFDWNVP--ALVAAILAMGMLNAV 134 Query: 126 FQLEGGAGLGLTYVTGALVKAGQLIAAALTGGAGWAWLPNLLLWAALVAGALAGAM 181 + G +GLTYVTGAL + G+ + L G W L+ W+ + GA+ GA+ Sbjct: 135 VEQVNGLPIGLTYVTGALSRFGRGLGRWLLGERRNGWRVQLVPWSGMCVGAVLGAL 190 >AGc254 [S] COG3619 Predicted membrane protein Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 206 Score = 82.8 bits (203), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 66/172 (38%), Positives = 92/172 (53%), Gaps = 4/172 (2%) Query: 22 DGIGYLHLGGLFVSFMSGNSTRLGVTLAEGHWQHALEALALIVLFVAGAAAGSLIVLSAL 81 D +G LHL G FVSFM+GN+TR ++L G + HA + L IV FVAG A G IV++ Sbjct: 3 DAVG-LHLSGDFVSFMTGNTTRAAISLEAGVYSHAAKLLFAIVAFVAGNAGG--IVVAHK 59 Query: 82 AHRQPLLLLFEAWLLTA-AAVAYAYGLPSAAVAAIVLAMGLENAVFQLEGGAGLGLTYVT 140 R L +L LL A AA+ +V AMG+ NA + G +GLTYVT Sbjct: 60 FDRSILAVLVGVGLLVAIAALLRGDAFALTQFYLVVFAMGMVNAAVEHIEGLPIGLTYVT 119 Query: 141 GALVKAGQLIAAALTGGAGWAWLPNLLLWAALVAGALAGAMAYGWINLAAIW 192 GAL + G+ I L G W ++ W +++GA+ GA+ + A+W Sbjct: 120 GALSRFGRGIGRFLLGERSLDWGIQIVPWLGMISGAICGAVLGVTLQSGALW 171 >BMEI0217 [S] COG3619 Predicted membrane protein Bme Brucella melitensis Length = 214 Score = 82.4 bits (202), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 79/212 (37%), Positives = 108/212 (50%), Gaps = 28/212 (13%) Query: 2 LESRRKLALACALSALAGYVDGIGYLHLGGLFVSFMSGNSTRLGVTLA-----EGH---W 53 + KL L L+A AG+VD I +L LGG F SFMSGN+T+LG +A +G W Sbjct: 1 MTPNSKLTLGLVLTASAGFVDAIAFLQLGGFFASFMSGNTTQLGTAIAGQPGVQGEVLVW 60 Query: 54 QHALEALALIVLFVAGAAAGSLIV--------LSALAHRQPLLLLFEAWLLTAAAVAYAY 105 A+ LIVLF +GA G+LIV +A +LLL A V Sbjct: 61 FPAI----LIVLFFSGAFLGTLIVRAYGRWGSFYVMASVVAILLLVSVLRREGALVI--- 113 Query: 106 GLPSAAVAAIVLAMGLENAVFQLEGGAGLGLTYVTGALVKAGQLIAAALTG-GAGWAWLP 164 V + AMG +NA Q G A LG+TYVTG L A +A +L G W WL Sbjct: 114 ----QPVLLLAAAMGAQNAAVQPIGAARLGVTYVTGTLFNAAADLACSLRGETPKWRWLQ 169 Query: 165 NLLLWAALVAGALAGAMAYGWINLAAIWFAAA 196 ++ +W +L+ GA+ G + + +I L A++ AA Sbjct: 170 HVAVWLSLMLGAVGGGLGHSFIGLDALFVPAA 201 >SMa1297 [S] COG3619 Predicted membrane protein Sme Sinorhizobium meliloti Length = 195 Score = 77.0 bits (188), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 60/160 (37%), Positives = 85/160 (53%), Gaps = 6/160 (3%) Query: 22 DGIGYLHLGGLFVSFMSGNSTRLGVTLAEGHWQHALEALALIVLFVAGAAAGSLIVLSAL 81 D IG + +G FVSFMSGN+TR V L +G L + +V FV G AAG +I ++ Sbjct: 3 DAIGLMSIGD-FVSFMSGNTTRASVALVQGDAAQGLLLIGGLVSFVLGNAAGVMI---SI 58 Query: 82 AHRQPLLLLFEAWLLTAAAVAYAYGLPSAAVAAIVLAMGLENAVFQLEGGAGLGLTYVTG 141 R LLF + LL AA+ G P +++ AMG NA + G +GLTYVTG Sbjct: 59 RFRPQAALLFVSALLACAALQE--GQPELRFVSLIFAMGAVNASVEQIEGLPVGLTYVTG 116 Query: 142 ALVKAGQLIAAALTGGAGWAWLPNLLLWAALVAGALAGAM 181 AL + G+ + G W+ ++ W + AGA+ GA+ Sbjct: 117 ALSRFGRGLGRWAMGVRNTQWIIQIVPWLGMFAGAIMGAV 156 >SMa1162 [S] COG3619 Predicted membrane protein Sme Sinorhizobium meliloti Length = 283 Score = 73.2 bits (178), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 69/204 (33%), Positives = 103/204 (50%), Gaps = 6/204 (2%) Query: 8 LALACALSALAGYVDGIGYLHLGGLFVSFMSGNSTRLGVTLAEGHWQHALEALALIVLFV 67 LAL A+S LAG D +G + LG FVSFMSGN+TR V L + + + + FV Sbjct: 77 LALVAAISFLAGMTDAMGLMSLGD-FVSFMSGNTTRASVALIQADVARGVLLIGGLSTFV 135 Query: 68 AGAAAGSLIVLSALAHRQPLLLLFEAWLLTAAAVAYAYGLPSAAVAAIVLAMGLENAVFQ 127 G A G ++ + H +LLF + + A A ++LAMG NA + Sbjct: 136 IGNAVGVMVSIRFRPHS---VLLFVS--VLLACAALLADRREMQFLLLILAMGAINASVE 190 Query: 128 LEGGAGLGLTYVTGALVKAGQLIAAALTGGAGWAWLPNLLLWAALVAGALAGAMAYGWIN 187 G +GLTYVTGAL + G+ + + G W+ ++ W ++AGA++GA I Sbjct: 191 QIEGLPVGLTYVTGALSRFGRGLGRWVMGIRNTHWVIQIVPWLGMMAGAISGAALVQEIG 250 Query: 188 LAAIWFAAAAAFALSALVAATAKR 211 A+W +AAA L+A+ +R Sbjct: 251 SLALWIPSAAALLLTAVAIQIPRR 274