BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0075.0046.p (410 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa0669 [V] COG1566 Multidrug resistance efflux pump Sme Sinorhi... 490 e-138 yiaV [V] COG1566 Multidrug resistance efflux pump Eco Escherichi... 98 4e-20 ZyiaV [V] COG1566 Multidrug resistance efflux pump EcZ Escherich... 97 6e-20 ECs4462 [V] COG1566 Multidrug resistance efflux pump Ecs Escheri... 97 6e-20 VCA0047 [V] COG1566 Multidrug resistance efflux pump Vch Vibrio ... 92 3e-18 >SMa0669 [V] COG1566 Multidrug resistance efflux pump Sme Sinorhizobium meliloti Length = 409 Score = 490 bits (1262), Expect = e-138, Method: Compositional matrix adjust. Identities = 239/410 (58%), Positives = 303/410 (73%), Gaps = 1/410 (0%) Query: 1 MLELMICSLVTILPDYLYRHYRQGKRFGKEITFFSVWYELRWGITGCLMLTISLITMIFY 60 MLE +ICSL+TI PDYL R Y QGKR G+EI +S+W+ELR+GIT CL+LTISLITMIFY Sbjct: 1 MLEFLICSLLTIFPDYLVRRYVQGKRVGREINLYSMWFELRYGITACLILTISLITMIFY 60 Query: 61 FHPSTTSAAIYFRTVPILPEGSGRVVEVNVDFTAPVKKGDVLFRLDSSKQEAALETAKRK 120 FHPST++ FRTV I+PE +GRV EV VD V G LFRLD S+Q AALETA+R+ Sbjct: 61 FHPSTSNVTAVFRTVTIMPESTGRVAEVYVDLNEKVSAGAPLFRLDDSEQRAALETARRR 120 Query: 121 IAEVDASLIAAQADVVKADAQIGEAKASLQQAKDELEVKSELQRRNPGIVPQRDIEKLQV 180 +AE++A I AQ+++ AD I +A+ QA +ELE + EL +RNP IV +R+IE+LQV Sbjct: 121 VAEIEAEAIVAQSELASADGLIAQAEGDYLQALNELETQVELNQRNPNIVARREIERLQV 180 Query: 181 LVDQRQSGVDAAGAAKQSAALRVSTLLPAEKASAEAARAQAQVDLDKTFVRAGVDGRVEQ 240 VD R A+ + K++ ++++LLPA+KASAEAA QAQV+LDKT VRAG G V+Q Sbjct: 181 AVDGRDGARAASISNKRTLETKIASLLPAQKASAEAALTQAQVELDKTIVRAGTAGVVQQ 240 Query: 241 FLVRPGDLVAQIMRPAGIMIPDEAGRNRLQAGFGQIEAQVMKPGMIAEATCMSKPWVIIP 300 F +R GD+V ++R AGI++P++ R L AGFGQIEAQVMK GMIAEATC+ KP+ IIP Sbjct: 241 FALRAGDIVNPMIRSAGILVPEQR-RIGLIAGFGQIEAQVMKAGMIAEATCIGKPFTIIP 299 Query: 301 LVVTSVQDFIAAGQFRASELLIDPQSLQKPGSILVFLEPLYKGGLDGVTPGSTCIVNAYT 360 +VVT VQD IAAGQ R ++ L+D Q L PG++ +LEPLY+G GV PGS+CI NAYT Sbjct: 300 MVVTEVQDVIAAGQIRPTDQLVDAQQLASPGTLTAYLEPLYEGQFSGVPPGSSCIANAYT 359 Query: 361 SNHDVIADPKTGTLKGLALHAVDATALVHALLLRIQALLLPIQTLVLSGH 410 SNHD + P T L LH VDA LVHA++LR+QALLLP+QTLV GH Sbjct: 360 SNHDALHAPGISTPHWLFLHVVDAVGLVHAMILRLQALLLPVQTLVFKGH 409 >yiaV [V] COG1566 Multidrug resistance efflux pump Eco Escherichia coli K12 Length = 378 Score = 97.8 bits (242), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 86/308 (27%), Positives = 154/308 (50%), Gaps = 28/308 (9%) Query: 41 RWGIT----GCLMLTISLITMIFYFHPSTTSAAIYFRTVPILPEGSGRVVEVNVDFTAPV 96 +W I G + + LI ++ Y HP T A ++P++P+ +G V+EV + Sbjct: 26 KWTIPTAALGGIFIVSGLILLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLI 85 Query: 97 KKGDVLFRLDSSKQEAALETAKRKIAEVDASLIAAQADVVKADAQIGEAKASLQQAKDEL 156 KKG+VLFRLD ++ +A ++ + A ++ A+ A++ E A+ QQAK Sbjct: 86 KKGEVLFRLDPTRYQA-------RVDRLMADIVTAEHKQRALGAELDEMAANTQQAKATR 138 Query: 157 E-VKSELQRRNPG----IVP--QRDIEKLQVLVDQRQSGVDAAGAAKQSAALRVSTLLPA 209 + E QR G + P +RDI+ + +++ V ++ A ++ ++ +L+ Sbjct: 139 DKFAKEYQRYARGSQAKVNPFSERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLG 198 Query: 210 EK---ASAEAARAQAQVDLDKTFVRAGVDGRVEQFLVRPGDLVAQI-MRPAGIMIPDEAG 265 E AS +A A+A+ +L++T VRA DG V Q L+RPG A + +RP + IPD+ Sbjct: 199 EHSQIASLKAQLAEAKYNLEQTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQ-- 256 Query: 266 RNRLQAGFGQIEAQVMKPGMIAEATCMSKPWVIIPLVVTSVQDFIAAGQFRASELLIDPQ 325 + ++ A F Q + PG AE + P + + ++ + G ++++ L Q Sbjct: 257 KRQIVAQFRQNSLLRLAPGDDAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTL---Q 313 Query: 326 SLQK-PGS 332 +L PGS Sbjct: 314 TLNTAPGS 321 >ZyiaV [V] COG1566 Multidrug resistance efflux pump EcZ Escherichia coli O157:H7 EDL933 Length = 378 Score = 97.1 bits (240), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 83/303 (27%), Positives = 152/303 (50%), Gaps = 18/303 (5%) Query: 41 RWGIT----GCLMLTISLITMIFYFHPSTTSAAIYFRTVPILPEGSGRVVEVNVDFTAPV 96 +W I G + + LI ++ Y HP T A ++P++P+ +G V+EV + Sbjct: 26 KWTIPTAALGGIFIVSGLILLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLI 85 Query: 97 KKGDVLFRLDSSKQEAALETAKRKI--AEVDASLIAAQADVVKADAQIGEAKASLQQAKD 154 KKG+VLFRLD ++ +A ++ I AE + A+ D + A+ Q +AKA+ + Sbjct: 86 KKGEVLFRLDPTRYQARVDRLMADIVTAEHKQRALGAELDEMAANTQ--KAKATRDKFAK 143 Query: 155 ELEVKSELQRRNPGIVPQRDIEKLQVLVDQRQSGVDAAGAAKQSAALRVSTLLPAEK--- 211 E + + + +RDI+ + +++ V ++ A ++ ++ +L+ E Sbjct: 144 EYQRYARGSQAKVNPFSERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQI 203 Query: 212 ASAEAARAQAQVDLDKTFVRAGVDGRVEQFLVRPGDLVAQI-MRPAGIMIPDEAGRNRLQ 270 AS +A A+A+ +L++T VRA DG V Q L+RPG A + +RP + IPD+ + ++ Sbjct: 204 ASLKAQLAEAKYNLEQTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQ--KRQIV 261 Query: 271 AGFGQIEAQVMKPGMIAEATCMSKPWVIIPLVVTSVQDFIAAGQFRASELLIDPQSLQK- 329 A F Q + PG AE + P + + ++ + G ++++ L Q+L Sbjct: 262 AQFRQNSLLRLAPGDDAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTL---QTLNTA 318 Query: 330 PGS 332 PGS Sbjct: 319 PGS 321 >ECs4462 [V] COG1566 Multidrug resistance efflux pump Ecs Escherichia coli O157:H7 Length = 378 Score = 97.1 bits (240), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 83/303 (27%), Positives = 152/303 (50%), Gaps = 18/303 (5%) Query: 41 RWGIT----GCLMLTISLITMIFYFHPSTTSAAIYFRTVPILPEGSGRVVEVNVDFTAPV 96 +W I G + + LI ++ Y HP T A ++P++P+ +G V+EV + Sbjct: 26 KWTIPTAALGGIFIVSGLILLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLI 85 Query: 97 KKGDVLFRLDSSKQEAALETAKRKI--AEVDASLIAAQADVVKADAQIGEAKASLQQAKD 154 KKG+VLFRLD ++ +A ++ I AE + A+ D + A+ Q +AKA+ + Sbjct: 86 KKGEVLFRLDPTRYQARVDRLMADIVTAEHKQRALGAELDEMAANTQ--KAKATRDKFAK 143 Query: 155 ELEVKSELQRRNPGIVPQRDIEKLQVLVDQRQSGVDAAGAAKQSAALRVSTLLPAEK--- 211 E + + + +RDI+ + +++ V ++ A ++ ++ +L+ E Sbjct: 144 EYQRYARGSQAKVNPFSERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQI 203 Query: 212 ASAEAARAQAQVDLDKTFVRAGVDGRVEQFLVRPGDLVAQI-MRPAGIMIPDEAGRNRLQ 270 AS +A A+A+ +L++T VRA DG V Q L+RPG A + +RP + IPD+ + ++ Sbjct: 204 ASLKAQLAEAKYNLEQTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQ--KRQIV 261 Query: 271 AGFGQIEAQVMKPGMIAEATCMSKPWVIIPLVVTSVQDFIAAGQFRASELLIDPQSLQK- 329 A F Q + PG AE + P + + ++ + G ++++ L Q+L Sbjct: 262 AQFRQNSLLRLAPGDDAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTL---QTLNTA 318 Query: 330 PGS 332 PGS Sbjct: 319 PGS 321 >VCA0047 [V] COG1566 Multidrug resistance efflux pump Vch Vibrio cholerae Length = 376 Score = 91.7 bits (226), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 80/304 (26%), Positives = 141/304 (46%), Gaps = 10/304 (3%) Query: 41 RW----GITGCLMLTISLITMIFYFHPSTTSAAIYFRTVPILPEGSGRVVEVNVDFTAPV 96 +W + G ++L +LI ++ Y HP T + + T PI+ GRVVEV V P+ Sbjct: 26 KWTVPTAVLGGVILIGTLILLMNYNHPFTQLGSQVYSTTPIVSGVRGRVVEVPVKPNQPL 85 Query: 97 KKGDVLFRLDSSKQEAALETAKRKIAEVDASLIAAQADVVKADAQIGEAKASLQQAKDEL 156 +GDVLFR+D EA + K K+ E + ++ + +A A + +A A +A+ E Sbjct: 86 TQGDVLFRIDPIPFEADVARLKAKVKEASQGALGLESTLKEAQAAVLKAIAERDKAQREY 145 Query: 157 EVKSELQRRNPGIVPQRDIEKLQVLVDQRQSGVDAAGAAKQSAALRVSTLLPAEKASAEA 216 + +R G ++ ++ + Q+ ++ A + ++ A + + + + E + Sbjct: 146 DRYQRGYQR--GAFTEQQMDTTRQTYKAAQAALEVAQSKQEQAQIALDSEVGGENTTVAQ 203 Query: 217 ARAQ---AQVDLDKTFVRAGVDGRVEQFLVRPGDLVAQIMRPAGIMIPDEAGRNRLQAGF 273 A+ A+ DL++T VRA DG V Q +RPG +++ + A +M A F Sbjct: 204 LLAELRKAEFDLEQTIVRAPTDGYVTQLALRPG-MMSVPLPLAPVMTFVHTEEKIYTAAF 262 Query: 274 GQIEAQVMKPGMIAEATCMSKPWVIIPLVVTSVQDFIAAGQFRASELLIDPQSLQKPGSI 333 Q Q ++PG AE + P + V V I Q +A L+ +L+ G + Sbjct: 263 RQNSLQRLQPGFAAEFMFRALPGKVFKGEVIEVLPAIGESQIQARGALLGTDALRTSGRV 322 Query: 334 LVFL 337 V L Sbjct: 323 FVTL 326