BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0075.0025.p (198 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2372 [C] COG0437 Fe-S-cluster-containing hydrogenase componen... 312 3e-85 VC1512 [C] COG0437 Fe-S-cluster-containing hydrogenase component... 286 2e-77 Cj1510c [C] COG0437 Fe-S-cluster-containing hydrogenase componen... 250 1e-66 PAE1263 [C] COG0437 Fe-S-cluster-containing hydrogenase componen... 99 8e-21 AF2385 [C] COG0437 Fe-S-cluster-containing hydrogenase component... 97 3e-20 >RSc2372 [C] COG0437 Fe-S-cluster-containing hydrogenase components 1 Rso Ralstonia solanacearum Length = 231 Score = 312 bits (799), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 145/193 (75%), Positives = 167/193 (86%), Gaps = 1/193 (0%) Query: 1 MARMKFLCDADRCIECNACVTACKNEHEVPWGINRRRVVTIADGKPG-ERSVSMACMHCT 59 MARMKF+CD++RCIECNAC TACKNEHEVPWG++RRRVVTI DG G E+S+S+ACMHC+ Sbjct: 1 MARMKFICDSERCIECNACTTACKNEHEVPWGVSRRRVVTINDGMVGAEKSISVACMHCS 60 Query: 60 DAPCAAVCPVNCFYTTADGVVLHSKDLCIGCGYCFYACPFGAPQYPKVGNFGSRGKMDKC 119 DAPC AVCPV+CFY T DGVVLH KD+CIGCGYC YACPFGAPQ+P G FG RGKMDKC Sbjct: 61 DAPCMAVCPVDCFYRTEDGVVLHDKDVCIGCGYCSYACPFGAPQFPSQGTFGVRGKMDKC 120 Query: 120 TYCAGGPEADGSKEEYEKYGANRLAEGKLPLCAEMCSTKSLLAGDGEIIAQIYKERVLKR 179 T+CAGGPE +GS E+EKYG NRLAEGKLPLCAEMCSTK+LL GDG++IA I + RV+KR Sbjct: 121 TFCAGGPEPNGSDAEFEKYGRNRLAEGKLPLCAEMCSTKALLGGDGDVIADILRNRVMKR 180 Query: 180 GYGSGAWGWKTAY 192 G G+ +GW TAY Sbjct: 181 GKGAELFGWGTAY 193 >VC1512 [C] COG0437 Fe-S-cluster-containing hydrogenase components 1 Vch Vibrio cholerae Length = 202 Score = 286 bits (732), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 136/200 (68%), Positives = 161/200 (80%), Gaps = 5/200 (2%) Query: 1 MARMKFLCDADRCIECNACVTACKNEHE--VPWGINRRRVVTIADGKPGERSVSMACMHC 58 MARMKFLCD RCIECN CVTACKNE++ + WGI RRRVVT+ DG+PGE S+S+ACMHC Sbjct: 1 MARMKFLCDTKRCIECNGCVTACKNENDDALEWGIQRRRVVTLNDGEPGENSISVACMHC 60 Query: 59 TDAPCAAVCPVNCFYTTADGVVLHSKDLCIGCGYCFYACPFGAPQYPKVGNFGSRGKMDK 118 TDAPC AVCP +CF T DG+VLH+KDLCIGCGYC +ACPFGAPQ+PK FG RGKMDK Sbjct: 61 TDAPCMAVCPADCFVHTEDGIVLHNKDLCIGCGYCLFACPFGAPQFPKQAAFGERGKMDK 120 Query: 119 CTYCAGGPEAD-GSKEEYEKYGANRLAEGKLPLCAEMCSTKSLLAGDGEIIAQIYKERVL 177 CT+CAGGPE + GS+EE KYGANR+AEGKLP+CA +CSTK+LLAGD E I+ I+++RV+ Sbjct: 121 CTFCAGGPETEPGSEEERRKYGANRIAEGKLPMCASLCSTKALLAGDAEKISDIFRQRVV 180 Query: 178 KRGYGSGAW--GWKTAYRET 195 +RG W G AY T Sbjct: 181 ERGAKGAGWTNGEDLAYDAT 200 >Cj1510c [C] COG0437 Fe-S-cluster-containing hydrogenase components 1 Cje Campylobacter jejuni Length = 213 Score = 250 bits (639), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 116/195 (59%), Positives = 144/195 (73%), Gaps = 5/195 (2%) Query: 3 RMKFLCDADRCIECNACVTACKNEHEVPWGINRRRVVTIADGKPG-ERSVSMACMHCTDA 61 R+KF CD +RCI+CN C AC HE+P I RRRV+T+ +G G E S S++CMHC DA Sbjct: 13 RLKFFCDNERCIDCNGCAVACDEAHELPIHIRRRRVITLNEGIQGKEVSTSISCMHCDDA 72 Query: 62 PCAAVCPVNCFYTTADGVVLHSKDLCIGCGYCFYACPFGAPQYPKVGNFGSRGKMDKCTY 121 PC+ VCPV+CFY ADG+VLH K++CIGCGYC YACPFGAPQ+PK FG++G MDKCT Sbjct: 73 PCSIVCPVDCFYIRADGIVLHDKEICIGCGYCLYACPFGAPQFPKDSVFGNKGIMDKCTM 132 Query: 122 CAGGPEADGSKEEYEKYGANRLAEGKLPLCAEMCSTKSLLAGDGEIIAQIYKERVLKRGY 181 CAGGPEA S++E E YG NR+AEGK+P+CA MCSTK+LL G+ I +IY R++ R Y Sbjct: 133 CAGGPEATNSEKERELYGQNRIAEGKVPVCAAMCSTKALLVGESSKIEEIYHNRLMNRNY 192 Query: 182 G----SGAWGWKTAY 192 G S + WK AY Sbjct: 193 GIPNPSESLEWKIAY 207 >PAE1263 [C] COG0437 Fe-S-cluster-containing hydrogenase components 1 Pya Pyrobaculum aerophilum Length = 214 Score = 98.6 bits (244), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 59/162 (36%), Positives = 78/162 (48%), Gaps = 22/162 (13%) Query: 3 RMKFLCDADRCIECNACVTACKNEHEVPWGINRRRVVTIADGKPGERSVSMACMHCTDAP 62 R D +C C AC+ AC E+ VP G+ R + V C HC +AP Sbjct: 34 RWAMYIDVSKCYGCYACMVACAAENNVPIGVFRTWIERHVTKGGTVIFVPKQCNHCENAP 93 Query: 63 CAAVCPVNCFYTTADGVVLHSKDLCIGCGYCFYACPFGAPQYPKVGNFGSRGKMDKCTYC 122 C CP Y T DG+VL + DLCIGCG C ACP+GA + V +G +DKCT+C Sbjct: 94 CVKPCPTGATYKTEDGLVLVNDDLCIGCGACIQACPYGARFFNPV-----KGVVDKCTFC 148 Query: 123 AGGPEADGSKEEYEKYGANRLAEGKLPLCAEMCSTKSLLAGD 164 ++R+ +GKLP C E C T + + GD Sbjct: 149 -----------------SHRIYQGKLPACVETCPTGARVFGD 173 >AF2385 [C] COG0437 Fe-S-cluster-containing hydrogenase components 1 Afu Archaeoglobus fulgidus Length = 182 Score = 96.7 bits (239), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 67/194 (34%), Positives = 89/194 (45%), Gaps = 33/194 (17%) Query: 1 MARMKFLCDADRCIECNACVTACKNEHEVPWGIN-RRRVVTIADGK-PGERSVSM--ACM 56 MAR + D ++C+ C ACVTAC E+ VP+ R +V + GK P ++ M C Sbjct: 1 MARYGMVIDLEKCLGCEACVTACIEENNVPYETGLRTQVHRVVTGKFPNVSTMYMHRICQ 60 Query: 57 HCTDAPCAAVCPVNCFYTTADGVVLHSKDLCIGCGYCFYACPFGAPQYPKVGNFGSRGKM 116 HC C VCP Y DG+VL DLCIGC YC ACP+ A R Sbjct: 61 HCEHPGCVHVCPTGASYVNEDGIVLIDYDLCIGCKYCMVACPYLARYI-----HPERHTP 115 Query: 117 DKCTYCAGGPEADGSKEEYEKYGANRLAEGKLPLCAEMCSTKSLLAGD-----GEIIAQI 171 DKCT+C +R+ EGKLP C E C + + GD E+ + Sbjct: 116 DKCTFC-----------------VHRVKEGKLPACVETCPANARIFGDLDDPNSEVAKLV 158 Query: 172 YKERVLKRG--YGS 183 + R + G YG+ Sbjct: 159 AENRAIPIGAEYGT 172