BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0070.0036.p (443 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa1115 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP fami... 403 e-112 CAC3329 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP fami... 150 5e-36 CAC0685 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP fami... 133 8e-31 DR1709 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP famil... 97 1e-19 RSp0027 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP fami... 89 1e-17 >SMa1115 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Sme Sinorhizobium meliloti Length = 547 Score = 403 bits (1035), Expect = e-112, Method: Compositional matrix adjust. Identities = 211/416 (50%), Positives = 288/416 (69%) Query: 10 DDKHDDAPAAKPRRQPFGFNRVLKTLGPGLITGASDDDPSGIGTYSQAGAQLGYGIGWTM 69 DD+ AKP +L LGPGLITGA+DDDPSGI TYSQAGAQ G + W M Sbjct: 116 DDRVQSGLLAKPVVYRLRRAALLDKLGPGLITGAADDDPSGIATYSQAGAQFGANMLWMM 175 Query: 70 LLTFPLMSAIQEISARVGRVTGHGIAGNVCRHYPGWLLAVVVTLLSIANTINIAADLSAM 129 +PLM +Q ISAR+GRV+GHG+A N+ R +P W++ +V LL IANTINI ADL+AM Sbjct: 176 FFLYPLMCTMQMISARIGRVSGHGLAANMRRIFPSWVVTSLVALLFIANTINIGADLAAM 235 Query: 130 ADAAKLLIGGPAILYVVLFGVTSVAAQIFMNYRRYVAILKWLTLSLFAYVAALAFAKVSW 189 AA+L++G L+ ++F V S+ Q+ + Y RYV LKWLTL LFAYV + ++ W Sbjct: 236 GAAAELVLGWGRHLFTLVFAVASLTVQVLVPYHRYVLYLKWLTLVLFAYVGVVFTIEIDW 295 Query: 190 TEALAGVLLPRISWSADYFTTIVAILGTTISPYLFFWQASQEAEEQRVDVTKHPLIERHY 249 +E ++ P+++ + + +VA+ GTTISPYL FWQAS+E E+ D T PLI+ Sbjct: 296 SETALRMVTPQLALTRETAIMVVAVFGTTISPYLLFWQASEEVEDDEADPTTDPLIDHPE 355 Query: 250 GARREFSRIRADTITGMAFSNVIALSIIVTAAASLHAAGKTDIQTSAQAAEALRPIAGPF 309 A + SRIR DT GMAF+N++A II+T A +LHAAG T+I+TSA AAEALRPIAG Sbjct: 356 QALVQLSRIRWDTYIGMAFANLVAFFIILTTALTLHAAGVTEIETSADAAEALRPIAGDL 415 Query: 310 AEVIFALGIVGTGLLAIPVLAGATAYAVGEGRRWRVGLTRRPKEAIAFYSVLALSGGIGI 369 A +F+LGI+GTGLLA+P+LAG+ AYAV E R W +GL +P+EA+ FY+V+ L+ IG+ Sbjct: 416 AFALFSLGIIGTGLLAVPILAGSAAYAVCESRGWPIGLEHKPREAVGFYTVIGLATLIGL 475 Query: 370 ALNFTPIDPIAALYWSAVVNGVLAVPVMVLLMFMARRRDVMDRFVVGGWLYWLGWL 425 A++++ +DPI AL+WSAV+NGV++VP+M +M + R+D M +FV L +GW Sbjct: 476 AVDYSDLDPIRALFWSAVLNGVVSVPLMAAMMIVVSRKDEMGQFVASFRLRVMGWF 531 >CAC3329 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Cac Clostridium acetobutylicum Length = 411 Score = 150 bits (380), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 104/391 (26%), Positives = 192/391 (49%), Gaps = 29/391 (7%) Query: 31 VLKTLGPGLITGASDDDPSGIGTYSQAGAQLGYGIGWTMLLTFPLMSAIQEISARVGRVT 90 +L +GPGLIT + +D GI TY+ GA GY + W +LL ++ IQE++AR+ VT Sbjct: 10 ILGIIGPGLITVNAGNDAGGIATYATVGASYGYKMLWGLLLITFSLAIIQEMNARMAVVT 69 Query: 91 GHGIAGNVCRHY-PGWLLAVVVTLLSIANTINIAADLSAMADAAKLL-----IGGPAILY 144 G G++ + +Y W L + +L IAN D + +A + +L I P + + Sbjct: 70 GKGLSDLIRENYGVKWSL-FAMCILFIANFGVCVGDFAGIAASLELFGISKYITVPIMAF 128 Query: 145 VVLFGVTSVAAQIFMNYRRYVAILKWLTLSLFAYVAALAFAKVSWTEALAGVLLPRISWS 204 +V F +T +Y + + T F Y+ +W + + P +S++ Sbjct: 129 LVWFVITK------GSYEKTEKVFLAFTFVFFTYIITCIKVHPNWNVVMKATITPSLSFT 182 Query: 205 ADYFTTIVAILGTTISPYLFFWQASQEAEEQRVDVTKHPLIERHYGARREFSRIRADTIT 264 Y T++ ++GTTI+PY+ F+ S ++ + +E+ + D Sbjct: 183 KGYVLTLIGMIGTTITPYMQFYLQSSVVDKGL--------------SVKEYKYEKIDVYL 228 Query: 265 GMAFSNVIALSIIVTAAASLHAAGKTDIQTSAQAAEALRPIAGPFAEVIFALGIVGTGLL 324 G + ++++ IIV A +L+ AG I ++ QAA AL+P+AG +A ++FA G+ G +L Sbjct: 229 GAIWGDLVSFFIIVCTAVTLYKAG-IKIDSAEQAAMALKPLAGNYATILFAAGLFGASVL 287 Query: 325 AIPVLAGATAYAVGEGRRWRVGLTRRPKEAIAFYSVLALSGGIGIALNFTP-IDPIAALY 383 A V+ +T YA+ E + GL EA FY + AL P + + + Sbjct: 288 ATMVIPLSTTYAICEAFGFESGLDNSFSEAKTFYGIFTFMILFSAALVLVPKLSLVGIML 347 Query: 384 WSAVVNGVLAVPVMVLLMFMARRRDVMDRFV 414 + + G+L+ +++ ++ + +D+M +++ Sbjct: 348 ITQQIAGILSPVILIFMVLLINNKDIMGKYI 378 >CAC0685 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Cac Clostridium acetobutylicum Length = 408 Score = 133 bits (335), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 102/392 (26%), Positives = 191/392 (48%), Gaps = 26/392 (6%) Query: 28 FNRVL---KTLGPGLITGASDDDPSGIGTYSQAGAQLGYGIGWTMLLTFPLMSAIQEISA 84 F+R+L +GPGLIT + +D G+ TYS G++ GY + W + ++ +QE++A Sbjct: 5 FSRLLFIFSIVGPGLITANAGNDAGGVTTYSVIGSKYGYSMLWGLFAIGIGLAVVQEMNA 64 Query: 85 RVGRVTGHGIAGNVCRHYPGWLLAVVVTLLSIANTINIAADLSAMADAAKLLIGGPAILY 144 R+ VTG G++ + Y L + +L IAN AD + +A + +L I Sbjct: 65 RMAVVTGKGLSDLIRERYGVKLAFFAMLVLMIANLGVCIADFAGIAASLQLFNINKYISV 124 Query: 145 VVLFGVTSVAAQIFM---NYRRYVAILKWLTLSLFAYVAALAFAKVSWTEALAGVLLPRI 201 +L ++ I M NY + + LTL+ F YV K +W+ + + P Sbjct: 125 PIL----AIMIWIIMTKGNYSKAEKVFLLLTLTFFGYVITAFMLKPNWSNVFSHISNPGG 180 Query: 202 SWSADYFTTIVAILGTTISPYLFFWQASQEAEEQRVDVTKHPLIERHYGARREFSRIRAD 261 + S +Y+ ++ ++GTTI+PY+ F+ S ++ + +E+ + D Sbjct: 181 TLSKNYWLDLIGMVGTTITPYMQFYLQSSIVDKGI--------------SLKEYGYEKLD 226 Query: 262 TITGMAFSNVIALSIIVTAAASLHAAGKTDIQTSAQAAEALRPIAGPFAEVIFALGIVGT 321 G + + AL I + + +L A I ++ AA AL+P+AG ++ V+F +G+ G Sbjct: 227 VYLGTVWGILTALFITICTSETLFKA-HMAISSAQDAAAALKPLAGQYSFVLFGVGLFGA 285 Query: 322 GLLAIPVLAGATAYAVGEGRRWRVGLTRRPKEAIAFYSVLALSGGIGIALNFTP-IDPIA 380 LA ++ +T+YAV E + GL + KEA F+ + + + P I I+ Sbjct: 286 SFLACCIIPLSTSYAVCEAFGFESGLDNKIKEAPVFFGLFFFMIITSVIIVLMPNISLIS 345 Query: 381 ALYWSAVVNGVLAVPVMVLLMFMARRRDVMDR 412 + ++ + GVL V++ ++ + +++M + Sbjct: 346 IILFTQQLAGVLCPVVLIFMIKLVNDKEIMGK 377 >DR1709 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Dra Deinococcus radiodurans Length = 436 Score = 96.7 bits (239), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 116/451 (25%), Positives = 195/451 (43%), Gaps = 51/451 (11%) Query: 9 PDDKHDDAPAAKPRRQ---PFGFNRVLKTLGPGLITGASDDDPSGIGTYSQAGAQLGYGI 65 P ++H DA A R P G R+L LGP +I + DP T + GA+ GY + Sbjct: 15 PPEQHLDARAGATLRGTAGPRGVRRILPFLGPAVIASIAYMDPGNFATNIEGGARYGYSL 74 Query: 66 GWTMLLTFPLMSAIQEISARVGRVTGHGIAGNVCRHYPG---WLLAVVVTLLSIANTINI 122 W +L + IQ +SA +G +G + + +P W + L+++A + Sbjct: 75 LWVILAANLMAMVIQNLSANLGIASGRNLPELIRERWPRPLVWFYWIQAELVAMATDL-- 132 Query: 123 AADLSAMADAAKLLIGGPAILYVVLFGVTSVAAQIFMNYRR------YVAILKWLTLSLF 176 A+ A A +LL G P V VT V +N ++ +A+ ++ + Sbjct: 133 -AEFLGAALAIQLLTGLPMFWGAV---VTGVVTFWLLNLQKRGTRPLELAVGAFVLMIGV 188 Query: 177 AYVAALAFAKVSWTEALAGVLLPRISWSADYFTTIVAILGTTISPYLFFWQASQEAEEQR 236 AY+ + A+ AG +PR+ + V I+G T+ P++ + ++ + Sbjct: 189 AYLVQVVLARPDLAAVGAG-FVPRLQGPGSAYLA-VGIIGATVMPHVIYLHSALTQGRIQ 246 Query: 237 VDVTKHPLIERHYGARREFSRI-RADTITGMAFSNVIALSIIVTAAASLHAAGKTDIQTS 295 D T+ +R R+ R D I M + +I +S++ AAA+ H + Sbjct: 247 TDTTEE---------KRRLVRLNRVDVIAAMGLAGLINMSMLAVAAATFHGKNVENAGDL 297 Query: 296 AQAAEALRPIAGPFAEVIFALGIVGTGL--LAIPVLAGATAYAVGEGRRWRVGLTRRPKE 353 A + L P+ GP A V+FA+ ++ +GL A+ +AG G + L R Sbjct: 298 TTAYQTLTPLLGPAASVLFAVALLASGLSSSAVGTMAGDVIMQGFMGFHIPLWLRRLITM 357 Query: 354 AIAFYSVLALSGGIGIALNFTPIDPIAALYWSAVVNGVLAVP-VMVLLMFMARRRDVMDR 412 AF +L +G +DP + L S V+ VP +V L+ RRDVM Sbjct: 358 LPAFIVIL-----LG-------MDPSSVLILSQVIL-CFGVPFALVPLLLFTARRDVMGA 404 Query: 413 FVVGGWLYWLGWLSTGAMALSVIAMGVGFLL 443 V +GW+ +A+ +IA+ G+LL Sbjct: 405 LVTRRSFTVIGWV----IAVIIIALN-GYLL 430 >RSp0027 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Rso Ralstonia solanacearum Length = 547 Score = 89.4 bits (220), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 116/420 (27%), Positives = 186/420 (44%), Gaps = 33/420 (7%) Query: 5 GTIPPDDKHDDAPAAKPRRQPFGFNRVLKTLGPGLITGASDDDPSGIGTYSQAGAQLGYG 64 GTI P HD P R + +L LGPGLI D+D GTY+QAG G Sbjct: 30 GTIAP---HDIGPRRGWRAR---LRTLLAILGPGLIVMVGDNDAGAFGTYAQAGQNYGTT 83 Query: 65 IGWTMLLTFPLMSAIQEISARVGRVTGHGIAGNVCRHYPGWLLAVVVTLLSIANTINIAA 124 + WT+LL P++ QE+ R+G VT G A + + + + V L + N + + Sbjct: 84 LLWTLLLLIPVLYVNQEMVLRLGAVTRVGHARLIFARFGRFWGSFSVIDLFLVNALTLVT 143 Query: 125 DLSAMADAAKLLIGGPAILYVVLFGVTSVAAQIFMNYRRYVAILKWLTLSLFAYVAALAF 184 + ++ A L G P V + + A ++RR+ L + A + LA Sbjct: 144 EFIGISLALAYL-GIPRHWGVCVSAALVLLAAATGDFRRFERFALALVVGSLALIPVLAM 202 Query: 185 AKVSWTEALAGVLLPRISWSA---DYFTTIVAILGTTISPYLFFWQASQEAEEQRVDVTK 241 A + +P + A + I+AI+GTT++P+ F+Q S +D Sbjct: 203 AHPPLGQMARDFFVPALPAGAPLSEVVLLIIAIVGTTVAPWQLFFQQSYV-----IDKRI 257 Query: 242 HPLIERHYGARREFSRIRADTITGMAFSNVIALSIIVTAAASLHAAGKTDIQTSAQA-AE 300 P R+ RAD G+ + V A++++ +A + H + T A A Sbjct: 258 TPRFIRYE---------RADLWLGIVLAVVGAVAMMAFSAQAFHGTPEFGRYTDALGTAV 308 Query: 301 ALRPIAGPFAEVIFALGIVGTGLLAIPVLAGATAYAVGEGRRWRVGLTRRPKEAIAFYSV 360 L AG +A ++FA+ ++ ++ ++ +TAYA+G+ R L R+P EA FY+V Sbjct: 309 GLERHAGRWAGILFAIALLDASIIGACAVSLSTAYAIGDVLAVRHSLHRKPTEARCFYAV 368 Query: 361 LALSGGIGIALNFTPIDPIAALYWSAV--VNGVLAVPVMVLLMFMARRRDVMDRFVVGGW 418 + L TP P+ L SAV + GVL V L+ + D+ V+G W Sbjct: 369 YFGLTLLAAGLVLTPGVPLGLLT-SAVQSLAGVLLPSATVFLLLLCN-----DKAVLGPW 422