BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0069.0003.p (223 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa1367 [IQR] COG1028 Dehydrogenases with different specificitie... 149 6e-36 SMb20750 [IQR] COG1028 Dehydrogenases with different specificiti... 94 3e-19 TVN0711 [IQR] COG1028 Dehydrogenases with different specificitie... 93 5e-19 PA4089 [IQR] COG1028 Dehydrogenases with different specificities... 90 5e-18 DR1943 [IQR] COG1028 Dehydrogenases with different specificities... 88 1e-17 >SMa1367 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 224 Score = 149 bits (375), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 104/210 (49%), Positives = 123/210 (58%), Gaps = 2/210 (0%) Query: 15 LNSVAEECRSKGAKVEVSTGDLAKSGA-GTALXATRAAFGPIDSIVHAAGFADRREFSSL 73 L +VA+E ++GA+V GDL+ G + A RAAFG +D IV AG A R F L Sbjct: 14 LRTVAKEVSARGAEVATELGDLSDPTVPGHLVQAARAAFGGLDQIVSNAGRAQRSSFGQL 73 Query: 74 PREALERAFAVMAAAFHEIAAAALSDLTRSAQGRAVAVSSFGPHRF-VPGATYPGSAAAK 132 L+ AF +M AF + AAL DL S QGR VAVSSF H F G +P S AAK Sbjct: 74 TDADLQTAFDMMPMAFFRLVDAALPDLRTSMQGRVVAVSSFVAHGFGTNGMHFPASGAAK 133 Query: 133 AALEVLVKSLAIELAASGATANAVMPGYTRKDPGLFGALDTATWERVAKANPMQRLAEPD 192 AALE L KSLA +LA G T N V PG+TRKD G A +A E P RL EP Sbjct: 134 AALEALAKSLAAQLAPVGVTVNCVAPGFTRKDTGGHAATSSAAMESARAVTPNGRLGEPI 193 Query: 193 EVAAVVAFLLSPEAGHVTGATLPVDGGLTL 222 +VA +VAFLLSP A H+TG + VDGGL L Sbjct: 194 DVAELVAFLLSPGARHITGQVMHVDGGLLL 223 >SMb20750 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 249 Score = 93.6 bits (231), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 81/233 (34%), Positives = 108/233 (46%), Gaps = 23/233 (9%) Query: 2 LHGQGAEATGLARLNSVAEECRSK----GAKVEVSTGDLAKSGAGTALXATRAAFGPIDS 57 L G GA R EE R + G ++ ++ GD+ + A + A G +D Sbjct: 26 LAGAGAWTVINGRNGQGLEEARKRLKGDGIEIGIAAGDITRD-APAIIAAAVEKTGGLDI 84 Query: 58 IVHAAGFADRR--------EFSSLPREALERAFAVMAAAFHEIAAAALSDLTRSAQGRAV 109 ++HA G DRR EF++L L A+AV A AL L RSA GR + Sbjct: 85 LIHAVGERDRRGTEAMEPHEFAALLNTDLTAAYAV--------AKTALPYLRRSAAGRLI 136 Query: 110 AVSSFGPHRFVPGATYPGSAAAKAALEVLVKSLAIELAASGATANAVMPGYTRKDPGLFG 169 V+S F A P AAK L L +SLA+EL A T NA+ PG+ + Sbjct: 137 FVTSIAA--FAARAGDPAYTAAKGGLAALTRSLAVELGADNLTVNAIAPGWFATETNAHL 194 Query: 170 ALDTATWERVAKANPMQRLAEPDEVAAVVAFLLSPEAGHVTGATLPVDGGLTL 222 A D A V P++R P+E+A FL SP A V G TL VDGG+T+ Sbjct: 195 AADPALKAFVDIRIPLKRWGRPEEIAPAAVFLASPAASFVNGITLTVDGGMTV 247 >TVN0711 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Tvo Thermoplasma volcanium Length = 256 Score = 92.8 bits (229), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 64/231 (27%), Positives = 115/231 (49%), Gaps = 12/231 (5%) Query: 1 MLHGQGAEATGLAR----LNSVAEECRSK-GAKVEVSTGDLA-KSGAGTALXATRAAFGP 54 +L G G T +R L ++ EE + K G ++ DL+ +S + R +G Sbjct: 24 VLLGYGYNITAFSRDESKLRALKEEMKLKFGREINTVVADLSVRSDLENVVSNHREKYGS 83 Query: 55 IDSIVHAAGFADRREFSSLPREALERAFAVMAAAFHEIAAAALSDLTRSAQGRAVAVSSF 114 ID++V G F + E + A ++M + + AL D+ R+ G V V+S Sbjct: 84 IDNLVVNYGDPKVSSFIEISDEDWDYAISMMLKSTIYLTREALRDMIRTKHGSIVYVTSM 143 Query: 115 GPHRFVPGATYPGSAAAKAALEVLVKSLAIELAASGATANAVMPGYTRKD---PGLFGAL 171 + G + SA+ ++A+ L K+L E++ +G N + GY D + + Sbjct: 144 TVREPIEG--FSISASLRSAVISLAKTLTHEVSQNGIRVNTIAQGYFNTDRFRDIMKREI 201 Query: 172 DTAT-WERVAKANPMQRLAEPDEVAAVVAFLLSPEAGHVTGATLPVDGGLT 221 D+ + +R+A P++R+ EP+E+ +V FLLS + ++ GA +P+DGG+T Sbjct: 202 DSLSGMKRLAGEIPLRRVGEPEEIGELVEFLLSSRSSYINGANIPIDGGIT 252 >PA4089 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Pae Pseudomonas aeruginosa Length = 253 Score = 89.7 bits (221), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 74/212 (34%), Positives = 106/212 (50%), Gaps = 8/212 (3%) Query: 13 ARLNSVAEECRSKGAKVEVSTGDLAKSGAGT-ALXATRAAFGPIDSIVHAAGFADRREFS 71 +R +V E R G E+ DL++ A A+ AT FG +D ++H A + + Sbjct: 41 SRGQAVLEAIRLGGGTAELLAVDLSEYDAARRAVSATLERFGQLDILLHNAAAFPQCALA 100 Query: 72 SLPREALERAFAVMAAAFHEIAAAALSDLTRSAQGRAVAVSSF-GPHRFVPGATYPGSAA 130 L LER AV + + AAL L RS GR + SS GP +PG ++ AA Sbjct: 101 ELDEGTLERTLAVNLKSCFRLTQAALPALRRSVAGRVLVTSSVTGPRTAIPGLSH--YAA 158 Query: 131 AKAALEVLVKSLAIELAASGATANAVMPGYTRKDPGLFGALDTATWERVAKANPMQRLAE 190 +KA + +++ A+ELA G T N V PG P L D A +A P+ R+ + Sbjct: 159 SKAGVNGFIRAAALELAGDGITVNGVEPGLV-ATPALGSLGDAAA---LAAHIPLGRVGQ 214 Query: 191 PDEVAAVVAFLLSPEAGHVTGATLPVDGGLTL 222 P ++A + FL S EA ++TG TL VDGG L Sbjct: 215 PLDIAHAMLFLASDEASYITGQTLVVDGGALL 246 >DR1943 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Dra Deinococcus radiodurans Length = 254 Score = 88.2 bits (217), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 75/223 (33%), Positives = 109/223 (48%), Gaps = 27/223 (12%) Query: 11 GLARLNSVAEECRSKGAKVEVSTGDLAK-SGAGTALXATRAAFGPIDSIVHAAGFA---- 65 G A VA + R+ G +V DL++ + AGT + AA G +D +V+ AG Sbjct: 45 GEAEAQQVAADIRAAGGAAQVFGADLSQPANAGTLVEDVIAALGRLDVLVNNAGITRDGL 104 Query: 66 ----DRREFSSLPREALERAFAVMAAAFHEIAAAALSDLTRSAQGRAVAVSSFGPHRFVP 121 ++ ++ + L AFA AAL + ++ GR V VSS V Sbjct: 105 AIRMKDEDWDAVLQTNLSSAFAA--------CRAALKHMMKNRSGRIVNVSSV-----VA 151 Query: 122 GATYPGSA---AAKAALEVLVKSLAIELAASGATANAVMPGYTRKDPGLFGALDTATWER 178 A PG A A+KA L L ++LA E G T NA+ PG+ D + L T ++ Sbjct: 152 LAGNPGQANYVASKAGLIGLTRALAKEYGGRGITVNAIAPGFIESD--MTAKLPEDTKKQ 209 Query: 179 VAKANPMQRLAEPDEVAAVVAFLLSPEAGHVTGATLPVDGGLT 221 P+ R +P+EVAA+VAFL S AG++TG T+ VDGG+ Sbjct: 210 YQANIPLARFGQPEEVAALVAFLASEGAGYITGQTIGVDGGMN 252