BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0067.0011.p (304 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc4592 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 239 4e-63 Cgl1134 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 236 7e-62 Cgl2996 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 229 5e-60 PA2507 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit ... 139 5e-33 Cgl2351 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 119 7e-27 >AGc4592 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 295 Score = 239 bits (611), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 120/260 (46%), Positives = 161/260 (61%), Gaps = 1/260 (0%) Query: 4 FNETELTAAVIRSFDQTPDPRAKFLLQELVKSLHDYVRRTDLTFEEWDHAIGFLTRTGQK 63 F E VI DPR K ++ + + LH+ V+ + T EEW AI FLT GQ Sbjct: 12 FVEERSAETVIARMRDCDDPRLKEIMAVVTRKLHEAVKEIEPTEEEWMKAIHFLTEVGQI 71 Query: 64 CTDIRQEFILLSDVLGVSMLVDAVNHRDRDGATETTVLGPFYVGEHKVTPHGTDISASLP 123 C + RQE+IL SD+LGVSMLVDA+NHR GA+E+TVLGPF+V + P G +I Sbjct: 72 CNEWRQEWILFSDILGVSMLVDAINHRKPSGASESTVLGPFHVADAPEMPMGANICLDGK 131 Query: 124 GERMFVQSRVTDLDGKPLANVPVDVWHADDDGFYDSQKPSYATEGPSSRARFITDADGRF 183 GE M V R+ D +G P+A +DVW A+D+GFYD Q+ + + R F+T DGR+ Sbjct: 132 GEDMVVTGRILDTEGAPVAGARIDVWQANDEGFYDVQQKGIQPDF-NLRGVFVTGEDGRY 190 Query: 184 FFRTIVPCSYPIPTDGPVGQMITQTNRHPMRPAHVHFLVNAKGHEPLITHVFVEGDKYID 243 +FR P YPIP DGPVG+++ RHP RPAH+H++V+A+ L+TH+F D YI Sbjct: 191 WFRAAKPKYYPIPDDGPVGRLLRAMGRHPYRPAHLHYIVSAESFTTLVTHIFDPDDPYIR 250 Query: 244 SDVVFGVKDELIAKVERRTD 263 SD VFGVK+ L+A +R D Sbjct: 251 SDAVFGVKESLLANFDRVED 270 >Cgl1134 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Cgl Corynebacterium glutamicum Length = 295 Score = 236 bits (601), Expect = 7e-62, Method: Compositional matrix adjust. Identities = 125/289 (43%), Positives = 171/289 (59%), Gaps = 6/289 (2%) Query: 3 QFNETELTAAVIRSFDQTPDPRAKFLLQELVKSLHDYVRRTDLTFEEWDHAIGFLTRTGQ 62 Q E +L V+ SFD +PR K +++ L LHD++R LT EEW++AI FLT+ G Sbjct: 8 QAVEEDLVERVLASFDSCENPRLKLVMKSLTVHLHDFIRDVRLTEEEWNYAIDFLTKVGH 67 Query: 63 KCTDIRQEFILLSDVLGVSMLVDAVNHRDRDGATETTVLGPFYVGEHKVTPHGTDISASL 122 D RQEF+LLSD LG SM AVN+ + ATE TV GPF+V + + +G DI+ Sbjct: 68 ITDDKRQEFVLLSDTLGASMQTIAVNNEAYEDATEATVFGPFFVDDAPLVQNGDDIAFGA 127 Query: 123 PGERMFVQSRVTDLDGKPLANVPVDVWHADDDGFYDSQKPSYATEGPSSRARFITDADGR 182 G+ +V+ V D +G P+ N ++VW D+DG YD Q YA E + RA +D +G Sbjct: 128 VGQPAWVEGTVKDTEGNPIPNARIEVWECDEDGLYDVQ---YADERSAGRAHLYSDENGE 184 Query: 183 FFFRTIVPCSYPIPTDGPVGQMITQTNRHPMRPAHVHFLVNAKGHEPLITHVFVEGDKYI 242 + F + P YPIP DGPVGQM+ R P+R AH+HF+V A L+TH+FVEGD + Sbjct: 185 YHFWGLTPVPYPIPHDGPVGQMLQAVGRSPVRCAHLHFMVTAPEKRTLVTHIFVEGDPQL 244 Query: 243 D-SDVVFGVKDELIAK-VERRTDPVMPDGQQ-SDGPWSLMTYEFHVKPG 288 + D VFGVKD LI K VE+ PDG+ D W+ ++ + PG Sbjct: 245 EIGDSVFGVKDSLIKKFVEQPAGTATPDGRDVGDQTWARTRFDIVLAPG 293 >Cgl2996 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Cgl Corynebacterium glutamicum Length = 301 Score = 229 bits (584), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 123/287 (42%), Positives = 168/287 (58%), Gaps = 6/287 (2%) Query: 6 ETELTAAVIRSFDQTPDPRAKFLLQELVKSLHDYVRRTDLTFEEWDHAIGFLTRTGQKCT 65 E L V++SFD +PR K L++ LV LHD++R LT +EW++AI FLT G Sbjct: 18 EENLVNRVLQSFDACENPRLKQLMESLVVHLHDFIRDVRLTEDEWNYAIDFLTAVGHITD 77 Query: 66 DIRQEFILLSDVLGVSMLVDAVNHRDRDGATETTVLGPFYVGEHKVTPHGTDISASLPGE 125 D RQEF+LLSD LG SM AVN+ + +TE TV GPF++ + G DI+ G+ Sbjct: 78 DKRQEFVLLSDTLGASMQTIAVNNEAYENSTEATVFGPFFLDDAPEVELGGDIAGGAQGQ 137 Query: 126 RMFVQSRVTDLDGKPLANVPVDVWHADDDGFYDSQKPSYATEGPSSRARFITDADGRFFF 185 +++ VTD +G P+ N ++VW D+DG YD Q YA E + RA TDA+G + F Sbjct: 138 AAWIEGTVTDTEGNPVPNARIEVWECDEDGLYDVQ---YADERMAGRAYMHTDANGDYRF 194 Query: 186 RTIVPCSYPIPTDGPVGQMITQTNRHPMRPAHVHFLVNAKGHEPLITHVFVEGDKYID-S 244 + P YPIP DGPVG M+ R P+R AH+HF+V A L+TH+FVEGD ++ Sbjct: 195 WGLTPVPYPIPHDGPVGNMLKAVGRSPVRCAHLHFMVTAPELRTLVTHIFVEGDPQLEIG 254 Query: 245 DVVFGVKDELIAKVERRTDPV-MPDGQQ-SDGPWSLMTYEFHVKPGG 289 D VFGVKD LI K E + PDG+ D W+ ++ + PG Sbjct: 255 DSVFGVKDSLIKKFEEQAPGTPTPDGRDLGDQTWARTRFDIVLAPGA 301 >PA2507 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Pae Pseudomonas aeruginosa Length = 310 Score = 139 bits (351), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 93/306 (30%), Positives = 150/306 (49%), Gaps = 23/306 (7%) Query: 10 TAAVIRSFDQTP-------DPRAKFLLQELVKSLHDYVRRTDLTFEEWDHAIGFLTRTGQ 62 TA V R F++ DPR K L++ ++ + +T +E+ A+ +L R G Sbjct: 8 TADVQRFFEEASGQLNERGDPRTKALVRRILDDTAKLIEEMQVTPDEFWKAVDYLNRLGS 67 Query: 63 KCTDIRQEFILLSDVLGVSMLVDAVN-HRDRDGATETTVLGPFYVGEHKVTPHGTDISAS 121 + L + + +L+DA + G T T+ GP YV ++ + Sbjct: 68 RQEAGLLAAGLGLEHY-LDLLLDAQDAEAGLTGGTPRTIEGPLYVAGAPLSDGEARMDDG 126 Query: 122 L-PGERMFVQSRVTDLDGKPLANVPVDVWHADDDG---FYDSQKPSYATEGPSSRARFIT 177 G MF+Q RV+ DG+PLA VDVWHA+ G ++DS + Y + R R T Sbjct: 127 RDAGTVMFLQGRVSGPDGQPLAGAIVDVWHANTQGTYSYFDSSQSEY-----NLRRRIRT 181 Query: 178 DADGRFFFRTIVPCSYPIPTDGPVGQMITQTNRHPMRPAHVHFLVNAKGHEPLITHVFVE 237 DADGR+ R+IVP Y P+DGP +++ + RH RPAH+HF V+A GH L T + + Sbjct: 182 DADGRYRARSIVPSGYGCPSDGPTQELLDRLGRHGQRPAHIHFFVSAPGHRHLTTQINLA 241 Query: 238 GDKYIDSDVVFGVKDELIAKVERRTDPVMP-DGQQSDGPWSLMTYEFHVKPGGGMAPTPL 296 GD+Y+ D + +D LI + DP D G ++ + ++F ++ +P P Sbjct: 242 GDRYLWDDFAYATRDGLIGDLRFNDDPAAARDRGVEGGRFAELDFDFQLQ----ASPAPA 297 Query: 297 GTKVTE 302 + ++ Sbjct: 298 AERRSQ 303 >Cgl2351 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Cgl Corynebacterium glutamicum Length = 285 Score = 119 bits (299), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 75/256 (29%), Positives = 124/256 (48%), Gaps = 18/256 (7%) Query: 15 RSFDQTPDPRAKFLLQELVKSLHDYVRRTDLTFEEWDHAIGFLTRTGQKCTDIRQEFILL 74 R T RA + +L+ ++ + ++T+EE+ ++ G+ E+ L Sbjct: 27 RVSSDTSKERANAIYVDLLAAIAQVAHKHEVTYEEYAVLKQWMIDVGEYG-----EWPLW 81 Query: 75 SDVLGVSMLVDAVNHRDRD-GATETTVLGPFYVGEHKVTPHGTDI------SASLPGERM 127 DV V ++ +N+ D T+ ++ GP+YV P ++ A P + Sbjct: 82 LDVF-VEHEIEEINYNRHDYTGTKGSIEGPYYVENSPKLPWDAEMPMRDKDRACTP---L 137 Query: 128 FVQSRVTDLDGKPLANVPVDVWHADDDGFYDSQKPSYATEGPSSRARFITDADGRFFFRT 187 + +VTDLDG L V++WHAD+DG+Y P + R +TD +GR+ +T Sbjct: 138 IFEGQVTDLDGNGLDGAEVELWHADEDGYYSQFAPGIPEW--NLRGTIVTDEEGRYKIKT 195 Query: 188 IVPCSYPIPTDGPVGQMITQTNRHPMRPAHVHFLVNAKGHEPLITHVFVEGDKYIDSDVV 247 + P Y IP DGP G I HP RPAH+H V+ G+ + T ++ EG +++++DV Sbjct: 196 LQPAPYQIPHDGPTGWFIESYGGHPWRPAHLHLRVSHPGYRTITTQLYFEGGEWVENDVA 255 Query: 248 FGVKDELIAKVERRTD 263 VK EL+ E D Sbjct: 256 TAVKPELVLHPETGED 271