BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0089.p (622 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value APE1307 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming... 303 7e-82 MT2242 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming)... 273 1e-72 Rv2187 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming)... 272 2e-72 HI0002 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming)... 264 5e-70 AF0200 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming)... 259 9e-69 >APE1307 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Ape Aeropyrum pernix Length = 651 Score = 303 bits (776), Expect = 7e-82, Method: Compositional matrix adjust. Identities = 195/608 (32%), Positives = 307/608 (50%), Gaps = 15/608 (2%) Query: 16 TLPQLLQARRIERPTAVAYRQKRAGEWISVSWRDYATQVCRLAGALQMAGLARGDRVAIM 75 T P LL+ R +P VA+R KR G W +WRDY +V A AL+ GL GD A + Sbjct: 22 TFPWLLEWRARTQPGRVAFRWKRYGIWRRYTWRDYYLRVAYAALALENLGLGPGDTGAFI 81 Query: 76 GDVSQEWLLADMATMCAGAVTVGVYFTSSVEEVNYYLGDSGARFAFVGDETQLRTLLAAR 135 W++ ++ G +G+Y S +EV Y L A+F V + QL +L Sbjct: 82 TFNRPSWVIYEVGVQLLGGRAMGIYRDSLSDEVGYLLDSGDAKFVLVEGQEQLDRVLD-- 139 Query: 136 TAATLEKIVVMDPSWTGELRA---KNIVSFAEFY----EPVSFDCDAFLREQVAVAKTNE 188 + ++KI+V + + R +V+F + + S D +++ + + + Sbjct: 140 SGVDVDKIIVDEAKGLHQYRGMVGSKLVTFGDVMRLGRKLYSDGGDQQVKKLMGELEPDM 199 Query: 189 LASIGYTSGTTGYPKGAMLTHLSLLAGGHVQTTFHPTIRTETQRLVVHLPMSHTVARAQA 248 + + TSGTTG PK AM++ ++LA H T P E V LP + + + Sbjct: 200 VCGLFTTSGTTGLPKLAMISFKNMLAMAHQLNTVDPV--REDWEYVSFLPTAWIGEQMMS 257 Query: 249 TTLPLLSGAVPHFGETTAGFAQTIREAKPTYYMAPPRFYQRFATQILSVVHSASAKQRKD 308 ++ G +F ET + RE P + +PPR ++R A I++ V A +R Sbjct: 258 IPYHMIGGFKVNFPETPHTLWRDFREIAPHFMFSPPRVWERIAKDIMARVDDADPIKRAA 317 Query: 309 YQLAMRIARKALDDRQA-GRA-PDQYLSDLFAACRRHIFGPLLAEIGFDELKIAYTSSAP 366 ++LA +I +A R GR+ P L+ +L + G + AYT A Sbjct: 318 FRLAYKIGYEAARRRLVKGRSRPPTLWRALYYLAYWLALRAVLDKAGLKRIVRAYTGGAM 377 Query: 367 MPSEVMTLWQLWGLNLKECYGQTEMVGGNLVQMVDWPKAGSVGVPVQDPAWETAVLEDGE 426 + + + + G+NLK+ YGQTE+ G +V D + +VG P+ P E + EDGE Sbjct: 378 IGEDYVIFYHSLGVNLKQIYGQTEVAGIAVVHPDDDVRPDTVGKPL--PLTEVKIAEDGE 435 Query: 427 MIVRGPGLFIGYWNKPEETRSALRDGWLYTGDIAEVAQDGNFKLVDRKKEIINTSGGKSV 486 +++R P + GY+ E T + DGWL TGD+ + +DG+ K++DR KEII S G V Sbjct: 436 ILMRSPAMMKGYYKNEEATAKTIVDGWLRTGDVGVLTEDGHLKVLDRAKEIIILSDGTKV 495 Query: 487 SPTQIENEIRQSPYISEAAVIGEGKKYLTALIEVDATATMDWARSRDTDIAQYADLAKSE 546 +P I+N+++ SPYI EAA++G GK YL AL+ +D WA R Y+DL++ Sbjct: 496 APQVIQNKLKFSPYIGEAAIVGSGKPYLAALVNIDFEIVSRWAERRRIPFTSYSDLSQKP 555 Query: 547 IVIRMIEGEVAKANNRLARAEQIKAFRILPEELSPENGVMTPTRKKRRKQLMERYRALID 606 V+++++ E+ K N RL E+I+ F L +E P++ MT TRK RR + RY LI+ Sbjct: 556 EVLQLLKREIEKVNRRLPEKERIRRFVSLFKEFHPDDEEMTRTRKLRRMVIESRYAGLIE 615 Query: 607 TLYDESEE 614 +Y EE Sbjct: 616 AIYSGDEE 623 >MT2242 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) MtC Mycobacterium tuberculosis CDC1551 Length = 603 Score = 273 bits (697), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 191/596 (32%), Positives = 305/596 (51%), Gaps = 35/596 (5%) Query: 27 ERPTAVAYRQKRAGEWISVSWRDYATQVCRLAGALQMAGLARGDRVAIMGDVSQEWLLAD 86 + P V Y++ G W V+ + A Q+ A L G+ GDRV I EW + D Sbjct: 31 DDPDYVIYQRLIDGVWTDVTCAEAANQIRAAALGLISLGVQAGDRVVIFSATRYEWAILD 90 Query: 87 MATMCAGAVTVGVYFTSSVEEVNYYLGDSGARFAFVGDETQLRTLLAARTAATLEKI-VV 145 A + GAVTV Y TSS E+V + L DS A F ET + A + ++ + V Sbjct: 91 FAILAVGAVTVPTYETSSAEQVRWVLQDSEAVVLFA--ETDSHATMVAELSGSVPALREV 148 Query: 146 MDPSWTGELRAKNIVSFAEFYEPVSFDCDAFLREQVAVAKTNELASIGYTSGTTGYPKGA 205 + + +G + +P A L ++A ++ + A++ YTSGTTG PKG Sbjct: 149 LQIAGSGPNALDRLTEAGASVDP------AELTARLAALRSTDPATLIYTSGTTGRPKGC 202 Query: 206 MLTHLSLLAGGHVQTTFHPTIRTETQRLVVHLPMSHTVARAQATTLPLLSGAVPHFGETT 265 LT +L+ +HPT+ + +RL+V LP++H +ARA +S A H + T Sbjct: 203 QLTQSNLVHEIKGARAYHPTLLRKGERLLVFLPLAHVLARA-------ISMAAFH-SKVT 254 Query: 266 AGFAQTIREA-------KPTYYMAPPRFYQRFATQILSVVHSASAKQRKDYQLAMRIARK 318 GF I+ KPT ++ PR +++ + ++A+A + + + +A A+ Sbjct: 255 VGFTSDIKNLLPMLAVFKPTVVVSVPRVFEKVYNT--AEQNAANAGKGRIFAIA---AQT 309 Query: 319 ALDDRQAGR--APDQYLSDLFAACRRHIFGPLLAEIGFDELKIAYTSSAPMPSEVMTLWQ 376 A+D +A P L A R ++ L A +G + A + AP+ + + ++ Sbjct: 310 AVDWSEACDRGGPGLLLRAKHAVFDRLVYRKLRAALG-GNCRAAVSGGAPLGARLGHFYR 368 Query: 377 LWGLNLKECYGQTEMVGGNLVQMVDWPKAGSVGVPVQDPAWETAVLEDGEMIVRGPGLFI 436 GL + E YG + GG + + K G+VG PV P + +DGE++VRG +F Sbjct: 369 GAGLTIYEGYGLSGTSGGVAISQFNDLKIGTVGKPV--PGNSLRIADDGELLVRGGVVFS 426 Query: 437 GYWNKPEETRSALRDGWLYTGDIAEVAQDGNFKLVDRKKEIINTSGGKSVSPTQIENEIR 496 GYW + T A DGW TGD+ V +DG + RKKEII T+GGK+V+P +E+++R Sbjct: 427 GYWRNEQATTEAFTDGWFKTGDLGAVDEDGFLTITGRKKEIIVTAGGKNVAPAVLEDQLR 486 Query: 497 QSPYISEAAVIGEGKKYLTALIEVDATATMDWA-RSRDTDIAQYADLAKSEIVIRMIEGE 555 P IS+A V+G+ K ++ ALI +D A W R+ T A DLA +I I+ Sbjct: 487 AHPLISQAVVVGDAKPFIGALITIDPEAFEGWKQRNSKTAGASVGDLATDPDLIAEIDAA 546 Query: 556 VAKANNRLARAEQIKAFRILPEELSPENGVMTPTRKKRRKQLMERYRALIDTLYDE 611 V +AN ++ AE I+ FRILP + + + G +TPT K +RK + E++ + I+ +Y++ Sbjct: 547 VKQANLAVSHAESIRKFRILPVDFTEDTGELTPTMKVKRKVVAEKFASDIEAIYNK 602 >Rv2187 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Mtu Mycobacterium tuberculosis H37Rv Length = 600 Score = 272 bits (696), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 191/596 (32%), Positives = 305/596 (51%), Gaps = 35/596 (5%) Query: 27 ERPTAVAYRQKRAGEWISVSWRDYATQVCRLAGALQMAGLARGDRVAIMGDVSQEWLLAD 86 + P V Y++ G W V+ + A Q+ A L G+ GDRV I EW + D Sbjct: 28 DDPDYVIYQRLIDGVWTDVTCAEAANQIRAAALGLISLGVQAGDRVVIFSATRYEWAILD 87 Query: 87 MATMCAGAVTVGVYFTSSVEEVNYYLGDSGARFAFVGDETQLRTLLAARTAATLEKI-VV 145 A + GAVTV Y TSS E+V + L DS A F ET + A + ++ + V Sbjct: 88 FAILAVGAVTVPTYETSSAEQVRWVLQDSEAVVLFA--ETDSHATMVAELSGSVPALREV 145 Query: 146 MDPSWTGELRAKNIVSFAEFYEPVSFDCDAFLREQVAVAKTNELASIGYTSGTTGYPKGA 205 + + +G + +P A L ++A ++ + A++ YTSGTTG PKG Sbjct: 146 LQIAGSGPNALDRLTEAGASVDP------AELTARLAALRSTDPATLIYTSGTTGRPKGC 199 Query: 206 MLTHLSLLAGGHVQTTFHPTIRTETQRLVVHLPMSHTVARAQATTLPLLSGAVPHFGETT 265 LT +L+ +HPT+ + +RL+V LP++H +ARA +S A H + T Sbjct: 200 QLTQSNLVHEIKGARAYHPTLLRKGERLLVFLPLAHVLARA-------ISMAAFH-SKVT 251 Query: 266 AGFAQTIREA-------KPTYYMAPPRFYQRFATQILSVVHSASAKQRKDYQLAMRIARK 318 GF I+ KPT ++ PR +++ + ++A+A + + + +A A+ Sbjct: 252 VGFTSDIKNLLPMLAVFKPTVVVSVPRVFEKVYNT--AEQNAANAGKGRIFAIA---AQT 306 Query: 319 ALDDRQAGR--APDQYLSDLFAACRRHIFGPLLAEIGFDELKIAYTSSAPMPSEVMTLWQ 376 A+D +A P L A R ++ L A +G + A + AP+ + + ++ Sbjct: 307 AVDWSEACDRGGPGLLLRAKHAVFDRLVYRKLRAALG-GNCRAAVSGGAPLGARLGHFYR 365 Query: 377 LWGLNLKECYGQTEMVGGNLVQMVDWPKAGSVGVPVQDPAWETAVLEDGEMIVRGPGLFI 436 GL + E YG + GG + + K G+VG PV P + +DGE++VRG +F Sbjct: 366 GAGLTIYEGYGLSGTSGGVAISQFNDLKIGTVGKPV--PGNSLRIADDGELLVRGGVVFS 423 Query: 437 GYWNKPEETRSALRDGWLYTGDIAEVAQDGNFKLVDRKKEIINTSGGKSVSPTQIENEIR 496 GYW + T A DGW TGD+ V +DG + RKKEII T+GGK+V+P +E+++R Sbjct: 424 GYWRNEQATTEAFTDGWFKTGDLGAVDEDGFLTITGRKKEIIVTAGGKNVAPAVLEDQLR 483 Query: 497 QSPYISEAAVIGEGKKYLTALIEVDATATMDWA-RSRDTDIAQYADLAKSEIVIRMIEGE 555 P IS+A V+G+ K ++ ALI +D A W R+ T A DLA +I I+ Sbjct: 484 AHPLISQAVVVGDAKPFIGALITIDPEAFEGWKQRNSKTAGASVGDLATDPDLIAEIDAA 543 Query: 556 VAKANNRLARAEQIKAFRILPEELSPENGVMTPTRKKRRKQLMERYRALIDTLYDE 611 V +AN ++ AE I+ FRILP + + + G +TPT K +RK + E++ + I+ +Y++ Sbjct: 544 VKQANLAVSHAESIRKFRILPVDFTEDTGELTPTMKVKRKVVAEKFASDIEAIYNK 599 >HI0002 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Hin Haemophilus influenzae Length = 607 Score = 264 bits (675), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 178/607 (29%), Positives = 297/607 (48%), Gaps = 33/607 (5%) Query: 21 LQARRIERPTAVAYRQKRAGEWISVSWRDYATQVCRLAGALQMAGLARGDRVAIMGDVSQ 80 +Q + R A R K G W +SW+++ Q+ +L+ AL + D++AI + Sbjct: 19 IQQQAKTRTNMTALRYKEHGLWRDISWKNFQEQLNQLSRALLAHNIDVQDKIAIFAHNME 78 Query: 81 EWLLADMATMCAGAVTVGVYFTSSVEEVNYYLGDSGARFAFVGDETQL-RTLLAARTAAT 139 W + D+AT+ A+TV +Y T++ ++ + L + + FVGD+ Q +TL A Sbjct: 79 RWTIVDIATLQIRAITVPIYATNTAQQAEFILNHADVKILFVGDQEQYDQTLEIAHHCPK 138 Query: 140 LEKIVVMDPSWTGELRAKNIVSFAEFYEPVSFDCDAFLR-----------EQVAVAKTNE 188 L+KIV M K+ + + +P+S ++F++ +++ + ++ Sbjct: 139 LQKIVAM----------KSTIQLQQ--DPLSCTWESFIKTGSNAQQDELTQRLNQKQLSD 186 Query: 189 LASIGYTSGTTGYPKGAMLTHLSLLAGGHVQTTFHPTIRTETQRLVV-HLPMSHTVARAQ 247 L +I YTSGTTG PKG ML + +L H T ++ Q + + LP SH RA Sbjct: 187 LFTIIYTSGTTGEPKGVMLDYANL---AHQLETHDLSLNVTDQDISLSFLPFSHIFERAW 243 Query: 248 ATTLPLLSGAVPHFGETTAGFAQTIREAKPTYYMAPPRFYQRFATQILSVVHSASAKQRK 307 A + L GA+ + E T + E +PT A PRFY++ +L V A ++ Sbjct: 244 AAYI-LHRGAILCYLEDTNQVRSALTEIRPTLMCAVPRFYEKIYAAVLDKVQKAPKLRQI 302 Query: 308 DYQLAMRIARKALDDRQAGRAPDQYLSDLFAACRRHIFGPLLAEIGFDELKIAYTSSAPM 367 + A+ + +K D R +A L FA + + L +G +K+ A + Sbjct: 303 MFHWAISVGQKYFDLRANNKAIPFLLKKQFALADKLVLSKLRQLLG-GRIKMMPCGGAKL 361 Query: 368 PSEVMTLWQLWGLNLKECYGQTEMVGGNLVQMVDWPKAGSVGVPVQDPAWETAVLEDGEM 427 + + G+N+K YG TE S+G + P E + E+ E+ Sbjct: 362 EPAIGLFFHAIGINIKLGYGMTETTATVSCWHDFQFNPNSIGTLM--PKAEVKIGENNEI 419 Query: 428 IVRGPGLFIGYWNKPEETRSAL-RDGWLYTGDIAEVAQDGNFKLVDRKKEIINTSGGKSV 486 +VRG + GY+ KPEET A DG+L TGD E + GN + DR KE++ TS GK + Sbjct: 420 LVRGGMVMKGYYKKPEETAQAFTEDGFLKTGDAGEFDEQGNLFITDRIKELMKTSNGKYI 479 Query: 487 SPTQIENEIRQSPYISEAAVIGEGKKYLTALIEVDATATMDWARSRDTDIAQYADLAKSE 546 +P IE++I + +I + A+I + KKY++ALI + ++A+ + +L K+ Sbjct: 480 APQYIESKIGKDKFIEQIAIIADAKKYVSALIVPCFDSLEEYAKQLNIKYHDRLELLKNS 539 Query: 547 IVIRMIEGEVAKANNRLARAEQIKAFRILPEELSPENGVMTPTRKKRRKQLMERYRALID 606 +++M E + LA EQ+K F +L + S + G +TPT K RRK ++ERYR I+ Sbjct: 540 DILKMFEHRINAVQKELAHFEQVKKFTLLSQAFSIKLGEITPTLKLRRKVILERYRKQIE 599 Query: 607 TLYDESE 613 +Y E Sbjct: 600 AMYHSQE 606 >AF0200 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Afu Archaeoglobus fulgidus Length = 601 Score = 259 bits (663), Expect = 9e-69, Method: Compositional matrix adjust. Identities = 190/603 (31%), Positives = 286/603 (47%), Gaps = 54/603 (8%) Query: 28 RPTAVAYRQKRAGEWISVSWRDYATQVCRLAGALQMAGLARGDRVAIMGDVSQEWLLADM 87 R +A+ Y G+ +++ ++ +V +L+ L +GL +GDRVAI D EW +AD Sbjct: 30 RVSAIGYWDD--GKLKYLTYEEFWERVRKLSKFLISSGLRKGDRVAIYADTRYEWEIADF 87 Query: 88 ATMCAGAVTVGVYFTSSVEEVNYYLGDSGARFAFVGDE--TQLRTLLAARTAATLEKIVV 145 A + AG V V V+ + E+V Y L DS +R F + + LEK+V Sbjct: 88 AVLTAGGVVVTVHSVLNREQVEYILRDSESRVVFTEKKYAENVPEDFEVYFLEELEKLV- 146 Query: 146 MDPSWTGELRAKNIVSFAEFYEPVSFDCDAFLREQVAVAKTNELASIGYTSGTTGYPKGA 205 GE+ S + EP ++LASI YTSGTTG PKGA Sbjct: 147 ------GEVSDDEFESRWKSVEP------------------DDLASIVYTSGTTGEPKGA 182 Query: 206 MLTHLSLLAGGHVQTTFHPTIRTETQRLVVHLPMSHTVARAQATTLPLLSGAVPHFGETT 265 MLTH + H + P E + +LP+SH R +S A + Sbjct: 183 MLTHWNWRFNCHSVMSITPFYPGEPH--ICYLPLSHVYQRL--VFFAGISRAATAVFCSP 238 Query: 266 AGFAQTIREAKPTYYMAPPRFYQRFATQILSVVHSASAKQRKDYQLAMRIARKALDDRQA 325 F +T KP + PR +R I+ V + A +K + + +A + Sbjct: 239 QQFLETSTAVKPVGLIVVPRILERVNAGIVEKVEKSPALAKKIFYWSRGVAIECGKRMSR 298 Query: 326 GRAPDQYLSDLFAACRRHIFGPLLAEIGFDELKIAYTSSAPMPSEVMTLWQLWGLNLKEC 385 G +L+ R +F + +G ++ +S+A + E+ ++ G+ + E Sbjct: 299 GEKYGFWLNIKRIIADRLVFSKIRENLGLTRIRFVCSSAAELQKELAYMFNGMGIPVIEG 358 Query: 386 YGQTEMVGGNLVQMVDWPKAGSVGVPVQDPAWETAVLEDGEMIVRGPGLFIGYWNKPEET 445 YG TE + + V K G+VG P+ P E A+ EDGE++VRG + GYW K ET Sbjct: 359 YGMTETAAPSNLNPVGRFKPGTVGPPI--PGIEEAIAEDGEILVRGDNVMKGYWRKEAET 416 Query: 446 RSAL-RDGWLYTGDIAEVAQDGNFKLVDRKKEIINTSGGKSVSPTQIENEIRQSPYISEA 504 R DGWL TGD+ E +DG + RKK II GK+VSP IE E+ ++P +S+A Sbjct: 417 RKTFTEDGWLKTGDLGEFDEDGYLVFLGRKKHIIVLDTGKNVSPVPIEEELLKNPLVSDA 476 Query: 505 AVIGEGKKYLTALIEVDATATMDWAR------SRDTDIAQYADLAKSEIVI---RMIEGE 555 +IG+GK Y+T LI + + +++A R I Q + + EIV ++E Sbjct: 477 VIIGDGKPYVTTLIVPNFSMLLEFADKNGIEYDRSRTIVQRSISGEEEIVAVDENLVENN 536 Query: 556 VAK---------ANNRLARAEQIKAFRILPEELSPENGVMTPTRKKRRKQLMERYRALID 606 K N+RLA+ E IK F+ILPE S E G +TPT KKRR +++RY I+ Sbjct: 537 AVKELYAKIVDDVNSRLAKHETIKKFKILPEAFSLEKGEITPTLKKRRHVILKRYEKFIE 596 Query: 607 TLY 609 +Y Sbjct: 597 EMY 599