BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0080.p (1137 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PAE2859 [C] COG0243 Anaerobic dehydrogenases, typically selenocy... 183 2e-45 AF2384 [C] COG0243 Anaerobic dehydrogenases, typically selenocys... 182 3e-45 VNG0829G [C] COG0243 Anaerobic dehydrogenases, typically selenoc... 177 2e-43 SMa1191_2 [C] COG1018 Flavodoxin reductases (ferredoxin-NADPH re... 150 2e-35 AF0176 [C] COG0243 Anaerobic dehydrogenases, typically selenocys... 142 5e-33 >PAE2859 [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Pya Pyrobaculum aerophilum Length = 816 Score = 183 bits (465), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 149/526 (28%), Positives = 243/526 (46%), Gaps = 47/526 (8%) Query: 8 CTLCGSQCGTLNVVENGQLVAVRNDPSHPTGQAVCPKGRAGPEIVAANNRIIYPMRRTRP 67 C CG+ CG L V NGQ + + +P HP +C + + ++ R+ P++R Sbjct: 49 CAACGAACG-LFFVRNGQRMYLLPNPKHPQ-PGMCFRPASALQLWNHPLRLKKPLKRVGE 106 Query: 68 KTDPDPGWVRISWDEALDLCAGKLLDVRERYGAEGVAFAVTTKSGTGIADSIEWIDRFV- 126 + + + + W+ AL+ A +L ++ +RYG E VAF D + WI F+ Sbjct: 107 RGEGK--FQEVDWETALNEIAQRLREIVQRYGPESVAFTRH--------DHMSWILPFIA 156 Query: 127 RLFGSPNIASSTDVCNWHRDVAHRFTFGVGMPTA---DYAHAELIVLWGHNPTSTWLAQA 183 + G+PNI S C+ +F G P++ DY +A I+L G N + + Sbjct: 157 SVIGTPNIISHEGTCHAASTAVRKFVLGASGPSSVDPDYENANYILLIGRNLDAA-MGHI 215 Query: 184 DAIARGRANGAAMIVVDPRATPLAIDADVWLQVRPGTDAALALGVSNLLIQEGGVDLEFL 243 + R G ++VVDPRA LA + W+ + PGTDAA L + ++I EG D FL Sbjct: 216 RRLTVAREKGVKVVVVDPRAPNLAYSSVEWIPINPGTDAAFVLSLIYVIISEGLYDAAFL 275 Query: 244 RRWTNAPMLIRESDGLLLRARDIPDRESDGTAQQFVAWDVRTNEPYIYDTT--RLLDVDT 301 +++TNAP LI+ DG L +D+ +GTA +YD L D Sbjct: 276 KKYTNAPFLIK-PDGQPLTQKDL---GQEGTAY------------LVYDNAAKSLAPHDK 319 Query: 302 ARRFALTGLYEVRGHHGEAIACRPAFDHFQRACAAWTPERVEQVTGVPRTKLKAAASLMA 361 A+ AL EV+ G I + AF A +TPE E++TG+P ++ A A Sbjct: 320 AQDPALDYEGEVQTPAG-VIKVKTAFRLLAERAAKYTPENAEKITGIPADTIRRIAREFA 378 Query: 362 SHSKIAYHAWTGIGQGSNATQIERAVATLYALRGSFDEIGGNRILGRQPANKVDSTDLLV 421 + +A W G N RA+ L AL G+ D+ GG L Q ++K S + Sbjct: 379 TSRGVAEDGWYTAKNG-NDFDAYRAILILNALVGNIDKRGG---LCFQESSKFPSAVTVF 434 Query: 422 ETQSCKTLGRDIRPMGPAAEGRVKPLD-------VYRAIVTSDPYPIRALVGFGSNHLLT 474 + + +G + P+ R+K + + AI+ PYP++AL ++ Sbjct: 435 QDRIETIIGHVLPPIKAQRLDRLKYPETPATFDALLDAIIEEKPYPVKALFIVATSPFHR 494 Query: 475 QGDTDEIRRGLEVVEFYVHTDIVETPVARYADVLLPASTPWEREGL 520 +T+++++ L+ +E V DI+ ++D +LP ERE + Sbjct: 495 DVNTEKLKKALQKLELIVAIDILPQDHIDWSDYVLPDLMFLEREEI 540 >AF2384 [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Afu Archaeoglobus fulgidus Length = 822 Score = 182 bits (463), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 163/582 (28%), Positives = 256/582 (43%), Gaps = 59/582 (10%) Query: 4 IQSFCTLCGSQCGTLNVVENGQLVAVRNDPSHPTGQA-VCPKGRAGPEIVAANNRIIYPM 62 + + C +C + C V +G++ + P HP +C +G AG + V +R+ P+ Sbjct: 42 VPNICAMCPAACSIQVEVRDGKVHRIHGTPDHPINNGKICARGNAGVQRVYNPDRLKKPL 101 Query: 63 RRTRPKTDPDPGWV--RISWDEALDLCAGKLLDVRERYGAEGVAFAVTTKSGTGIADSIE 120 RT K W SW+EAL L A K+ + RE E + T Sbjct: 102 IRTGEKGT----WSFREASWEEALSLIASKVKEYREMGHPEYIGMLGGWLPCTYYKP--- 154 Query: 121 WIDRFVRLFGSPNIASSTD-VCNWHRDVAHRFTFGVGMP---TADYAHAELIVLWGHNPT 176 + F+ G+PN + +C + + + +G G DY +A +++ N Sbjct: 155 FFKAFLAALGTPNGGGVPEALCFLSKALGWKSAYGFGAHPELLTDYENARYVIMLRRN-- 212 Query: 177 STWLAQADAIARGRANGA-----AMIVVDPRATPLAIDADVWLQVRPGTDAALALGVSNL 231 +A + ++ G G ++V+DPR + A ADVWL ++PGTD A L + N+ Sbjct: 213 ---VAGSISVVHGWRLGQNRRKFKLVVLDPRYSETAAKADVWLPIKPGTDLAFLLAMMNV 269 Query: 232 LIQEGGVDLEFLRRWTNAPMLIRESDGLLLRARDIPDRESDGTAQQFVAWDVRTNEPY-I 290 +I E D FL +++NAPML++ DG + F WD + Y + Sbjct: 270 IINEKLYDSNFLAKYSNAPMLLK--DG-----------------KPFKVWDENGKKKYLV 310 Query: 291 YDTTR--LLDVDTARRFALTGLYEVRGHHGEAIACRPAFDHFQRACAAWTPERVEQVTGV 348 +D + ++ D+A AL G YEV G PAF+ +R A +TPE E +T + Sbjct: 311 FDLAKGAAVEHDSAMLPALEGEYEVEGEK-----VIPAFEALKRRVAEYTPEWAEGITDI 365 Query: 349 PRTKLKAAASLMASHSKIAYHAWTGIGQGSNATQIERAVATLYALRGSFDEIGGNRILGR 408 K++ A A + W G + N+ RA A + AL GS + GG G Sbjct: 366 EAKKIREIAREFALRRGVIDSGWHG-PKYRNSLLTWRAAAIVNALVGSVNNDGGLLFTGL 424 Query: 409 QPANKVDSTDLLVE--TQSCKTLGRDIRPMGPAAEGRVKPLDVYRAIVTSDPYPIRALVG 466 A V S + E +QS + + R + A G Y AIV DPYPI+ L Sbjct: 425 --AQFVTSKESTTEAPSQSVLRMWAEKRGIATAFLGHTVQA-FYDAIVNEDPYPIKMLFV 481 Query: 467 FGSNHLLTQGDTDEIRRGLEVVEFYVHTDIVETPVARYADVLLPASTPWEREGLRFGFGI 526 G N L+ + + L+ ++F V DI+ YADV+LP ST E++ F I Sbjct: 482 VGHNLLMNMPERQKWEEALKKLDFMVAVDILPQDHLYYADVVLPESTYIEKDDPL--FPI 539 Query: 527 SEAAQNLIQLRPAVLAPRGEARSDLEIVFALAERLGMGDKFF 568 + A R + P + + +E++ +A RLG D FF Sbjct: 540 AYAPAFGFHTRVKAIEPIYDTKHVIEMMVEIASRLGTEDTFF 581 >VNG0829G [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Hbs Halobacterium sp. NRC-1 Length = 837 Score = 177 bits (448), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 202/777 (25%), Positives = 308/777 (39%), Gaps = 93/777 (11%) Query: 1 MAEIQSFCTLCGSQCG---TLNV-VENGQLVAVRND-PSHPTGQAVCPKGRAGPEIVAAN 55 + E Q T C C L+V V +GQ+ V P+ T + C G + V Sbjct: 50 LGEDQVVKTACSPNCRGKCPLDVFVRDGQIKKVEQQVPAAKTFKRGCTLGMTHLQRVYNA 109 Query: 56 NRIIYPMRRTR-PKTDPDPG-------WVRISWDEALDLCAGKLLDVRERYGAEGVAFAV 107 +R+ YPM+RT DP P + RI+WD+ALDL A + + YG + + Sbjct: 110 DRLKYPMKRTSWSPDDPQPDQRGADAQFERIAWDDALDLVADGIQRAKREYGPRSLLW-- 167 Query: 108 TTKSGTGIADSIEWIDRFVRLFGSPNIASSTDVCNWHRDVAHRFT-----FGVGMPTA-- 160 SG+G I R L G + + +V F GV MP Sbjct: 168 --HSGSGDG-GITGYRRLKELVGGLQDDFTYGIDT---NVGQGFNRVTGEGGVFMPPTNT 221 Query: 161 --DYAHAELIVLWGHNP-TSTWLAQADAIARGRANGAAMIVVDPRATPLAIDADVWLQVR 217 D+ +AE I++WG + S + A+ I + NGA ++VVDP T A AD+WL ++ Sbjct: 222 ADDWVNAETIIIWGSDIFASQFQMDAEWILDAKRNGAKLVVVDPVYTNTAEKADLWLPIK 281 Query: 218 PGTDAALALGVSNLLIQEGGVDLEFLRRWTNAPMLIRESDGLLLRARDIPDR-ESDGTAQ 276 PG D LAL + + + D FLR TNAP L+R DG LL + DG Sbjct: 282 PGKDTHLALAMMQYIFEHDHYDEAFLRSRTNAPALVRADDGTLLDPASVTATPPEDG--- 338 Query: 277 QFVAWDVRTNEPYIYDTTRLLDVDTARRFALTGLYEVRGHHGEAIACRPAFDHFQRACAA 336 V ++ T P ++ +T FAL G + + G + ++ Sbjct: 339 -IVVFNTETGSP------EVVPAETNGPFALFGEWTIDG-----TTVHTGLTALREQASS 386 Query: 337 WTPERVEQVTGVPRTKLKAAASLMASHSKIAYHAWTGIGQGSNATQIERAVATLYALRGS 396 + P+ V G+ ++ AA +A+ G+G+ + ATL AL G Sbjct: 387 YPPQAVADTAGLAAADIETAADWLATRGPGGIMPSYGVGRYLYGHVFGQTYATLLALTGD 446 Query: 397 FDEIGGNRILGRQPANKVDSTDLLVETQSCKTLGRDIRPMGPAAEGRVKPLDVYRAIVTS 456 + G I + P+ D + +ET P G A V + Sbjct: 447 YGRHG--NIHAQHPS--YDGS--YLETGDWND------PDGAAGVDTYGYNRVLDLLANG 494 Query: 457 DPYPIRALVGFGSNHLLTQ-GDTDEIRRGLEVVEFYVHTDIVETPVARYADVLLPASTPW 515 DP + + G SN L Q + D + ++ V DI TP R AD++LPA+ + Sbjct: 495 DPVQTKFMYGMNSNMLGNQFPERDRWLDAMSNLDTVVWADIYHTPTTRQADIILPAAHWF 554 Query: 516 EREGLRFGFGISEAAQNLIQLRPAVLAPRGEARSDLEIVFALAERLGMGDKFFHGDIDAG 575 E E L ++ + R P EAR D I+ LA+RLG GDKF D Sbjct: 555 ETEDL-----LTTYTHPNLSYRTKAHDPLWEARDDYYIMAGLAQRLGHGDKFPDDKHDVL 609 Query: 576 WNYMLAPLGITVQDLRANGGKIIRPIEHAVRKYGHVDAWDRVAGFQTETRRVELYSELLL 635 ++ ++ LR G + E Y F TE+ R+ +Y + Sbjct: 610 DRFVKNDDRLSWDALRETG--TVATDETPTVAY--------TDEFGTESGRITVYDDDAP 659 Query: 636 RNH-----------QSPVPIHAEE-----HVDRSLYPFTLISMKNGVFCHSQHRGIASLR 679 + P P+ A H D YP + + HSQ + LR Sbjct: 660 VEEGPALPDDGVSLEVPKPLEARTADDWAHADE--YPLLFMQKHSKWRIHSQWANVPWLR 717 Query: 680 RRASRPTIRLHGSAARLKNINDGHSVEVTSRHGSTRFVAEIDNTLASDVLAADFGWW 736 + P + +H A + I+DG V V + GS A+ ++ + ++ D GWW Sbjct: 718 EINTEPQLDIHPKDATRRGIDDGEYVRVHNDRGSVVVRAKYNDGIQPGLVNTDQGWW 774 >SMa1191_2 [C] COG1018 Flavodoxin reductases (ferredoxin-NADPH reductases) family 1 Sme Sinorhizobium meliloti Length = 252 Score = 150 bits (379), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 79/248 (31%), Positives = 133/248 (53%), Gaps = 4/248 (1%) Query: 795 GFRRFIVSDRHAESDSALAISLAPQETGALPDYEPGQYVRIRVARPDTGELLTRAYSLTG 854 G+RRFIV +++ ESD + L P + G + D+EPGQY + V P G R YSL+ Sbjct: 2 GWRRFIVREKNPESDVITSFVLEPADGGPVADFEPGQYTSVAVQVPKLGYQQIRQYSLSD 61 Query: 855 AAGIRNRSEYRICVGRASAGASDASTSLSSLIHETLKVGDMVEVEAPSGAFTMPTVSSVP 914 + N YRI V R G +SSL+H+ + VGD +++ AP G F + ++ P Sbjct: 62 SP---NGRSYRISVKREDGGLG-TPGYVSSLLHDEINVGDELKLAAPYGNFYIDVSATTP 117 Query: 915 LILMATGIGITPFMSLLNSLDGDQMPSVTLLYGNRNGSEHAFKRQLRALAQRLPRLVVRT 974 ++L++ G+G+TP +S+L V ++G RN + HA + +L+ ++ P + Sbjct: 118 IVLISGGVGLTPMVSMLKKALQTPPRKVVFVHGARNSAVHAMRDRLKEASRTYPDFKLFI 177 Query: 975 FYSRPRASDVPGADFDVHGRLDAAQIDASLVMERPRFYLCGSDTMVTSTTRSLVSFGVPK 1034 FY P +D+ G D+D G +D ++ S++++ +Y+CG + L+ G+ + Sbjct: 178 FYDEPLPTDIEGRDYDFAGLVDVEKVKDSILLDDADYYICGPVPFMRMQHDKLLGLGITE 237 Query: 1035 FDIHTEVF 1042 IH EVF Sbjct: 238 ARIHYEVF 245 >AF0176 [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Afu Archaeoglobus fulgidus Length = 896 Score = 142 bits (357), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 193/829 (23%), Positives = 328/829 (39%), Gaps = 118/829 (14%) Query: 4 IQSFCTL-CGSQCGTLNVVENGQLVAVRNDPSHPTGQAV---CPKGRAGPEIVAANNRII 59 ++S C+ C CG +V NG++ + +P + C +G++ ++ +R+ Sbjct: 42 VRSVCSPNCTGACGFDALVYNGRIETLTQAADYPEPEYNPRGCLRGQSMMNLIYGPDRLK 101 Query: 60 YPMRRTRPKTDPDPGWVRISWDEALDLCAGKLLDVRERYGAEGVAFAVTTKSGTGIADSI 119 P+ R + + + +SWDEALD A +L D+ +R+G E VA ++ GTG Sbjct: 102 RPLIRVGKRGEGK--FKAVSWDEALDYVADRLADIMKRHGPESVAISIQV-PGTGYVHKG 158 Query: 120 EWIDRFVRLFGSPNIASSTDVCNWHRDVAHRFTFGVG---MPTADYAHAELIVLWGHNPT 176 + R +FG + T N TFGV + ++ +++ I ++G N Sbjct: 159 A-MMRLASMFGWSALHGYT--MNGDLPAFWSQTFGVQTEEFESLEWTNSKYIAIFGSNIM 215 Query: 177 STWLAQADAIARGRANGAAMIVVDPRATPLAIDADVWLQVRPGTDAALALGVSNLLIQEG 236 T L A + GA ++VVDP +P A AD W+Q+ P TD+A AL ++ +I+ Sbjct: 216 VTRLPDAKFVNIAAERGAKVVVVDPNFSPTAAKADEWVQINPSTDSAFALAIAREIIENR 275 Query: 237 GVDLEFLRRWTNAPMLIRESDGLLLRARDIPDRESDGTAQQ-------FVAWDVRTNEPY 289 D EF++ +T+ P+L+R L A+++ + E FV +DV NE + Sbjct: 276 LYDEEFIKTYTDMPLLVRMDTQKRLMAKEVKELEEIAGKLNVPEYRDVFVVYDV--NEGF 333 Query: 290 IYDTTRLLDVDTARRFALTGLYEVRGHHGEAIACRPAFDHFQRACAAWTPERVEQVTGVP 349 L+ D A+ G +EV G+ + +P F + +TPE+V ++ P Sbjct: 334 KPVNPETLERDFEP--AIEGEFEVELVDGKRVKVKPVFQLLKEEVQKYTPEKVAEIITPP 391 Query: 350 -RTK------LKAAASLMASHSKIAYHAWTGIGQGSNATQIERAVATLYALRGSFDEIGG 402 R K +K A MA+ + Q + RA+A + AL G+ + G Sbjct: 392 NRNKGEYVDMIKRLAFEMATVKPLHIIYGASNYQWYHGDLKGRALALIVALTGNLGKSGA 451 Query: 403 NRILGRQPANKVDSTDLLVETQSCKTLGRDIRPMGPAAEGRVKPLDVYRAIVTSDPYP-- 460 A + L S K + + ++ PYP Sbjct: 452 GI---STYAGQFKIRWPLGAWWSFKGRKNKWVTYLLWMNKEYRQSEEFKKYNRETPYPKN 508 Query: 461 -IRALVGFGSNHLLTQGDTDEIRRGLEV-----VEFYVHTDIVETPVARYADVLLPASTP 514 ++A + FG N+ Q + R +E +E V D T ++ADV+LP Sbjct: 509 DVKAFL-FGWNNPFDQHNM--ANRMIEYAENGDLELIVAIDFQMTTSCKWADVVLPGVAW 565 Query: 515 WEREGLRFGFGISEAAQNLIQLRPAVLAPRGEARSDLEIVFALAERL--GMGDKFFHGDI 572 +E+ L + +QL+ + P E ++ I LA+RL D+ I Sbjct: 566 YEKYEL-----TATILHPYVQLQQPAIEPLFECMPEIWIFKELAKRLVQKWNDEELKSSI 620 Query: 573 ------------------DAGWNYMLAPLGITVQDLRA------NGGKIIRPIEHAVRKY 608 D W++ LA L+A GG+++ I Sbjct: 621 SEFYPDPELFDEEERARKDGTWSFRLARNIAHKASLKAVELMLKTGGELVEGITLEKLLK 680 Query: 609 G------------HVDAWDRV------------AGFQTETR-----RVELYSEL-LLRNH 638 G + W+++ Q R R+E Y E + + Sbjct: 681 GPVRLNLPTPNKRQIPFWEQINLKKPFPPQSYPVPLQKTARFVKSGRIEFYKEEDVFIDL 740 Query: 639 QSPVPIHAEEHVD---------RSLYPFTLISMKNGVF-CHSQH-RGIASLRRRASRPTI 687 +P+H + VD + Y + I+ +N ++ HS H I L + RP + Sbjct: 741 GETLPVHKDPFVDTEYRLNPEAKRKYRYFYIT-RNALYRVHSTHSNNITMLELQDFRPRV 799 Query: 688 RLHGSAARLKNINDGHSVEVTSRHGSTRFVAEIDNTLASDVLAADFGWW 736 LH A+ I +G VEV + G A +D L V+ + GWW Sbjct: 800 WLHPITAKENGIAEGDLVEVYNDRGRVYGYAVLDEGLHPSVIIFEQGWW 848