BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0019.p (217 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEII0739 [L] COG3145 Alkylated DNA repair protein Bme Brucella ... 270 2e-72 ZalkB [L] COG3145 Alkylated DNA repair protein EcZ Escherichia c... 253 2e-67 ECs3101 [L] COG3145 Alkylated DNA repair protein Ecs Escherichia... 253 2e-67 alkB [L] COG3145 Alkylated DNA repair protein Eco Escherichia co... 252 4e-67 RSc2568 [L] COG3145 Alkylated DNA repair protein Rso Ralstonia s... 241 1e-63 >BMEII0739 [L] COG3145 Alkylated DNA repair protein Bme Brucella melitensis Length = 212 Score = 270 bits (690), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 128/212 (60%), Positives = 156/212 (73%), Gaps = 4/212 (1%) Query: 4 DLFETIGDARPSRETIADGAVLLRGFVRPFEAELIPALRVIVKQAPFRHLITPGGHRMSV 63 DLF+ + +RE +A GAVLLRGF E ++ A+ + APFRH+ TPGG+RMSV Sbjct: 3 DLFDNLN----TREIMAPGAVLLRGFALACEESILSAVARVCAAAPFRHMTTPGGYRMSV 58 Query: 64 AMTNCGSLGWVSDPGGYRYDPIDPDSGQPWPEMPEVLRRLAAGAAEEAGFNGFAPEACLI 123 AMTNCG +GWV+D GYRY P DP++G+PWP MP+ LA AA +AG+ F P+ACLI Sbjct: 59 AMTNCGPVGWVTDRTGYRYSPADPETGKPWPSMPDTFLALAQDAARQAGYPDFTPDACLI 118 Query: 124 NRYVPGAKLSLHQDKDELDYRAPIVSISLGLPAIFLFGGLKRSDTPRRYRLEHGDVAVWG 183 NRY PGA+LSLHQDKDE ++ PIVS+SLGLPA F FGG+KR+D +Y L HGDV VWG Sbjct: 119 NRYKPGARLSLHQDKDERNFSNPIVSVSLGLPATFQFGGMKRTDPITKYILHHGDVVVWG 178 Query: 184 GPSRLFYHGVAPLADGEHSVLGRQRINLTFRK 215 GPSRLFYHG+ PL GEH LG R+NLTFRK Sbjct: 179 GPSRLFYHGILPLKSGEHERLGPFRLNLTFRK 210 >ZalkB [L] COG3145 Alkylated DNA repair protein EcZ Escherichia coli O157:H7 EDL933 Length = 216 Score = 253 bits (647), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 122/212 (57%), Positives = 146/212 (68%) Query: 5 LFETIGDARPSRETIADGAVLLRGFVRPFEAELIPALRVIVKQAPFRHLITPGGHRMSVA 64 + + DA P +E +A GAV+LR F +LI + + Q+PFR ++TPGG+ MSVA Sbjct: 1 MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVA 60 Query: 65 MTNCGSLGWVSDPGGYRYDPIDPDSGQPWPEMPEVLRRLAAGAAEEAGFNGFAPEACLIN 124 MTNCG LGW + GY Y PIDP + +PWP MP+ L AA AG+ F P+ACLIN Sbjct: 61 MTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQRAATAAGYPDFQPDACLIN 120 Query: 125 RYVPGAKLSLHQDKDELDYRAPIVSISLGLPAIFLFGGLKRSDTPRRYRLEHGDVAVWGG 184 RY PGAKLSLHQDKDE D RAPIVS+SLGLPAIF FGGLKR+D +R LEHGDV VWGG Sbjct: 121 RYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVWGG 180 Query: 185 PSRLFYHGVAPLADGEHSVLGRQRINLTFRKV 216 SRLFYHG+ PL G H + R NLTFR+ Sbjct: 181 ESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQA 212 >ECs3101 [L] COG3145 Alkylated DNA repair protein Ecs Escherichia coli O157:H7 Length = 216 Score = 253 bits (647), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 122/212 (57%), Positives = 146/212 (68%) Query: 5 LFETIGDARPSRETIADGAVLLRGFVRPFEAELIPALRVIVKQAPFRHLITPGGHRMSVA 64 + + DA P +E +A GAV+LR F +LI + + Q+PFR ++TPGG+ MSVA Sbjct: 1 MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVA 60 Query: 65 MTNCGSLGWVSDPGGYRYDPIDPDSGQPWPEMPEVLRRLAAGAAEEAGFNGFAPEACLIN 124 MTNCG LGW + GY Y PIDP + +PWP MP+ L AA AG+ F P+ACLIN Sbjct: 61 MTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQRAATAAGYPDFQPDACLIN 120 Query: 125 RYVPGAKLSLHQDKDELDYRAPIVSISLGLPAIFLFGGLKRSDTPRRYRLEHGDVAVWGG 184 RY PGAKLSLHQDKDE D RAPIVS+SLGLPAIF FGGLKR+D +R LEHGDV VWGG Sbjct: 121 RYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVWGG 180 Query: 185 PSRLFYHGVAPLADGEHSVLGRQRINLTFRKV 216 SRLFYHG+ PL G H + R NLTFR+ Sbjct: 181 ESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQA 212 >alkB [L] COG3145 Alkylated DNA repair protein Eco Escherichia coli K12 Length = 216 Score = 252 bits (644), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 122/212 (57%), Positives = 146/212 (68%) Query: 5 LFETIGDARPSRETIADGAVLLRGFVRPFEAELIPALRVIVKQAPFRHLITPGGHRMSVA 64 + + DA P +E +A GAV+LR F +LI + + Q+PFR ++TPGG+ MSVA Sbjct: 1 MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVA 60 Query: 65 MTNCGSLGWVSDPGGYRYDPIDPDSGQPWPEMPEVLRRLAAGAAEEAGFNGFAPEACLIN 124 MTNCG LGW + GY Y PIDP + +PWP MP+ L AA AG+ F P+ACLIN Sbjct: 61 MTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQRAATAAGYPDFQPDACLIN 120 Query: 125 RYVPGAKLSLHQDKDELDYRAPIVSISLGLPAIFLFGGLKRSDTPRRYRLEHGDVAVWGG 184 RY PGAKLSLHQDKDE D RAPIVS+SLGLPAIF FGGLKR+D +R LEHGDV VWGG Sbjct: 121 RYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVWGG 180 Query: 185 PSRLFYHGVAPLADGEHSVLGRQRINLTFRKV 216 SRLFYHG+ PL G H + R NLTFR+ Sbjct: 181 ESRLFYHGIQPLKAGFHPLTIDCRYNLTFRQA 212 >RSc2568 [L] COG3145 Alkylated DNA repair protein Rso Ralstonia solanacearum Length = 218 Score = 241 bits (614), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 127/212 (59%), Positives = 151/212 (71%) Query: 4 DLFETIGDARPSRETIADGAVLLRGFVRPFEAELIPALRVIVKQAPFRHLITPGGHRMSV 63 DLF A R + + AV+LRGF L+ A+ I +QAPFRH++TPGG MSV Sbjct: 5 DLFADHAPADDRRIALGEAAVVLRGFALAEATALLAAIDDIARQAPFRHMVTPGGFEMSV 64 Query: 64 AMTNCGSLGWVSDPGGYRYDPIDPDSGQPWPEMPEVLRRLAAGAAEEAGFNGFAPEACLI 123 A+TNCG+LGW SD GYRY DP +GQPWP +P+ RLA AA AGF GF P+ACLI Sbjct: 65 ALTNCGALGWTSDRRGYRYAARDPQTGQPWPPLPDCFLRLARDAAAAAGFPGFTPDACLI 124 Query: 124 NRYVPGAKLSLHQDKDELDYRAPIVSISLGLPAIFLFGGLKRSDTPRRYRLEHGDVAVWG 183 NRYVPGA+LSLHQDKDE DY APIVS+SLG+PA+FL+GG +R+D R L HGDV VWG Sbjct: 125 NRYVPGARLSLHQDKDEQDYGAPIVSVSLGMPAMFLWGGHRRTDKTLRVPLFHGDVVVWG 184 Query: 184 GPSRLFYHGVAPLADGEHSVLGRQRINLTFRK 215 GP RL YHGV PL + H +LG QRINLT R+ Sbjct: 185 GPDRLRYHGVLPLKEAAHPLLGAQRINLTLRR 216