BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0012.p (268 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1105 [Q] COG3971 2-keto-4-pentenoate hydratase Sty Salmonella... 370 e-102 BMEII0138 [Q] COG3971 2-keto-4-pentenoate hydratase Bme Brucella... 363 e-100 DRA0122 [Q] COG3971 2-keto-4-pentenoate hydratase Dra Deinococcu... 362 e-100 YPO1766 [Q] COG3971 2-keto-4-pentenoate hydratase Ype Yersinia p... 358 9e-99 PA4127 [Q] COG3971 2-keto-4-pentenoate hydratase Pae Pseudomonas... 357 2e-98 >STM1105 [Q] COG3971 2-keto-4-pentenoate hydratase Sty Salmonella typhimurium LT2 Length = 267 Score = 370 bits (950), Expect = e-102, Method: Compositional matrix adjust. Identities = 167/256 (65%), Positives = 208/256 (81%) Query: 12 AERLDGAEKTRKQIRQLSLEYPRITLEDAYAIQKAWVEMKVAQGRVVKGHKIGLTSKAMQ 71 A+RL+ AEK R+QIR +SL+YP IT+EDAYA+Q+ WV +K+A+GR +KGHKIGLTSKAMQ Sbjct: 11 AQRLNQAEKQREQIRAVSLDYPNITIEDAYAVQREWVNIKIAEGRTLKGHKIGLTSKAMQ 70 Query: 72 SALNIDEPDSGILLDDMFFADGGLVPSDRFIATRVEAELAFVMKHRLAGPNCTLFDVLNA 131 ++ I EPD G LLDDMFF DGG +P+DRFI R+E ELAFV+ L GP+CTLFDV NA Sbjct: 71 ASSQISEPDYGALLDDMFFHDGGDIPTDRFIVPRIEVELAFVLAKPLRGPHCTLFDVYNA 130 Query: 132 TDFVVPALEILDTRVERVDPETKATRKIFDTIADNAANAGIVLGGRPIRPLDADLRWIGA 191 TD+V+PALE++D R +DPET+ RK+FDTI+DNAANAG++LGGRPI+P + DLRWI A Sbjct: 131 TDYVIPALELIDARSHNIDPETQRPRKVFDTISDNAANAGVILGGRPIKPDELDLRWISA 190 Query: 192 LCFRNGQLEETGLAAGVLNHPATAVAWLANKIAPNGLALEAGQVVLAGSFIRPIETRKGD 251 L +RNG +EETG+AAGVLNHPA VAWLANK+AP + LEAGQ++L GSF RP+ RKGD Sbjct: 191 LLYRNGVIEETGVAAGVLNHPANGVAWLANKLAPYDVQLEAGQIILGGSFTRPVPARKGD 250 Query: 252 TIQADYGPYGSVSCHF 267 T DYG G++SC F Sbjct: 251 TFHVDYGNMGAISCRF 266 >BMEII0138 [Q] COG3971 2-keto-4-pentenoate hydratase Bme Brucella melitensis Length = 267 Score = 363 bits (932), Expect = e-100, Method: Compositional matrix adjust. Identities = 174/265 (65%), Positives = 215/265 (81%) Query: 3 LSKDDIRAAAERLDGAEKTRKQIRQLSLEYPRITLEDAYAIQKAWVEMKVAQGRVVKGHK 62 L+KD+I AAA LDGAEKTR+Q+ LSL++P +T++DAY++Q AWV+ K+A GR V G K Sbjct: 2 LTKDEIEAAAAALDGAEKTRRQVGLLSLKHPGMTMDDAYSVQAAWVKQKIAAGRRVIGWK 61 Query: 63 IGLTSKAMQSALNIDEPDSGILLDDMFFADGGLVPSDRFIATRVEAELAFVMKHRLAGPN 122 IGLTSKAMQ ALNID PDSG+LLDDM F DG ++P+DRFI R+EAE+AF MK L GPN Sbjct: 62 IGLTSKAMQYALNIDIPDSGVLLDDMAFEDGAVIPADRFIQPRIEAEIAFTMKAPLKGPN 121 Query: 123 CTLFDVLNATDFVVPALEILDTRVERVDPETKATRKIFDTIADNAANAGIVLGGRPIRPL 182 ++FDVLNATD++ PALEILDTR+ RVDPETK TR I DTI+DNAANAGIV+GGR +R Sbjct: 122 VSVFDVLNATDYITPALEILDTRILRVDPETKKTRSIVDTISDNAANAGIVIGGRQMRAD 181 Query: 183 DADLRWIGALCFRNGQLEETGLAAGVLNHPATAVAWLANKIAPNGLALEAGQVVLAGSFI 242 D+RW+GA+ RN Q+EETGL AGVLNHPA + WLAN++A G +EAGQ+VLAGSFI Sbjct: 182 PVDMRWMGAIVSRNAQVEETGLGAGVLNHPARGIVWLANRLALYGEGIEAGQIVLAGSFI 241 Query: 243 RPIETRKGDTIQADYGPYGSVSCHF 267 +E R GDTI AD+GPYG+++CHF Sbjct: 242 CLVEARHGDTINADFGPYGTIACHF 266 >DRA0122 [Q] COG3971 2-keto-4-pentenoate hydratase Dra Deinococcus radiodurans Length = 268 Score = 362 bits (930), Expect = e-100, Method: Compositional matrix adjust. Identities = 170/266 (63%), Positives = 208/266 (78%) Query: 3 LSKDDIRAAAERLDGAEKTRKQIRQLSLEYPRITLEDAYAIQKAWVEMKVAQGRVVKGHK 62 L+ + IR AA RL AE R+QI LS +YP IT+EDAY +Q AWV K+AQGR V GHK Sbjct: 2 LTDEQIRDAAHRLQEAETNRRQIGHLSRQYPDITIEDAYRVQDAWVSHKLAQGRRVYGHK 61 Query: 63 IGLTSKAMQSALNIDEPDSGILLDDMFFADGGLVPSDRFIATRVEAELAFVMKHRLAGPN 122 IGLTS+AMQ + NI+EPD G+LLDDM FA+G +P+ RFI RVE ELAF++ L+GP+ Sbjct: 62 IGLTSRAMQKSSNINEPDYGVLLDDMVFAEGSEIPAGRFIVPRVEVELAFLLDKDLSGPH 121 Query: 123 CTLFDVLNATDFVVPALEILDTRVERVDPETKATRKIFDTIADNAANAGIVLGGRPIRPL 182 CT+FDVL AT +V PA+EI+D R++++DPETKATRK+FDTI+DNAANAGIV GGRP+RP Sbjct: 122 CTVFDVLGATAYVFPAIEIIDARIQQLDPETKATRKVFDTISDNAANAGIVTGGRPVRPF 181 Query: 183 DADLRWIGALCFRNGQLEETGLAAGVLNHPATAVAWLANKIAPNGLALEAGQVVLAGSFI 242 D DLRW+GAL ++NG +EETG+AAGVLNHPA VAWLANK A +G LEAGQV+LAGSF Sbjct: 182 DFDLRWLGALLYKNGVIEETGVAAGVLNHPANGVAWLANKYAAHGRRLEAGQVILAGSFT 241 Query: 243 RPIETRKGDTIQADYGPYGSVSCHFA 268 RP+E GD ADYGP G V+ F Sbjct: 242 RPVEADPGDVFHADYGPLGGVTLRFG 267 >YPO1766 [Q] COG3971 2-keto-4-pentenoate hydratase Ype Yersinia pestis Length = 267 Score = 358 bits (918), Expect = 9e-99, Method: Compositional matrix adjust. Identities = 158/265 (59%), Positives = 209/265 (78%) Query: 3 LSKDDIRAAAERLDGAEKTRKQIRQLSLEYPRITLEDAYAIQKAWVEMKVAQGRVVKGHK 62 L++ I A++L AE+ +QIRQ+SL+YP IT+EDAYAIQ+ WV MK+ QG++++GHK Sbjct: 2 LAQKTIIQIAQQLYQAEQCGEQIRQVSLDYPMITIEDAYAIQRQWVAMKIQQGQILRGHK 61 Query: 63 IGLTSKAMQSALNIDEPDSGILLDDMFFADGGLVPSDRFIATRVEAELAFVMKHRLAGPN 122 IGLTSKAMQ++ I+EPD G LLD MFFADG +P DRFI R+E ELAFV+ L+GPN Sbjct: 62 IGLTSKAMQTSSQINEPDYGTLLDQMFFADGSDIPIDRFIVPRLEVELAFVLDKPLSGPN 121 Query: 123 CTLFDVLNATDFVVPALEILDTRVERVDPETKATRKIFDTIADNAANAGIVLGGRPIRPL 182 CTLFDV NATDFV+PALE++D R ++DP+++ R + DTI+DNAANAG++LGGRP++P Sbjct: 122 CTLFDVYNATDFVIPALELIDARSHQIDPQSQRPRNVCDTISDNAANAGVILGGRPVKPT 181 Query: 183 DADLRWIGALCFRNGQLEETGLAAGVLNHPATAVAWLANKIAPNGLALEAGQVVLAGSFI 242 + DLRW+ AL +RNG +EE+G+AA VLNHPA VAWLANK++ + LE GQ++L GSF Sbjct: 182 ELDLRWVSALMYRNGVIEESGVAAAVLNHPANGVAWLANKLSAYDIPLEVGQIILGGSFT 241 Query: 243 RPIETRKGDTIQADYGPYGSVSCHF 267 RP+ R+GDT DYGP G++SCHF Sbjct: 242 RPVPARRGDTFHVDYGPLGAISCHF 266 >PA4127 [Q] COG3971 2-keto-4-pentenoate hydratase Pae Pseudomonas aeruginosa Length = 267 Score = 357 bits (915), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 165/260 (63%), Positives = 206/260 (79%) Query: 8 IRAAAERLDGAEKTRKQIRQLSLEYPRITLEDAYAIQKAWVEMKVAQGRVVKGHKIGLTS 67 I+ AA RLD AE++R+Q+RQ SL+YP I +EDAYAIQ+AWVE K+A GRV+KGHKIGLTS Sbjct: 7 IQEAANRLDAAERSRQQVRQFSLDYPDIAIEDAYAIQRAWVERKIADGRVLKGHKIGLTS 66 Query: 68 KAMQSALNIDEPDSGILLDDMFFADGGLVPSDRFIATRVEAELAFVMKHRLAGPNCTLFD 127 +AMQ + NI EPD G LLDDMFF +G +P RFI RVE ELAF++ L GP CTLFD Sbjct: 67 RAMQVSSNISEPDYGALLDDMFFEEGSDIPFQRFIVPRVEVELAFILGKPLKGPGCTLFD 126 Query: 128 VLNATDFVVPALEILDTRVERVDPETKATRKIFDTIADNAANAGIVLGGRPIRPLDADLR 187 VL AT++V+PALEI+D R+++VDP+TK TRK+FDTI+DNAANAG+V+GGR +RP D DLR Sbjct: 127 VLEATEWVIPALEIIDARIQQVDPQTKVTRKVFDTISDNAANAGVVMGGRAVRPGDIDLR 186 Query: 188 WIGALCFRNGQLEETGLAAGVLNHPATAVAWLANKIAPNGLALEAGQVVLAGSFIRPIET 247 + A+ +RNG +EE+G++A VLNHPA VAWLANK+AP G+ LEAGQV+L GSF RP+ Sbjct: 187 RVPAILYRNGVIEESGVSAAVLNHPAKGVAWLANKLAPYGVCLEAGQVILGGSFTRPVAA 246 Query: 248 RKGDTIQADYGPYGSVSCHF 267 GDT DY GS+SC F Sbjct: 247 APGDTFHVDYDLLGSISCRF 266