BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0066.0005.p (361 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr9117 Non COG 617 e-176 ybiC [C] COG2055 Malate/L-lactate dehydrogenases Eco Escherichia... 242 1e-63 ZybiC [C] COG2055 Malate/L-lactate dehydrogenases EcZ Escherichi... 241 2e-63 ECs0879 [C] COG2055 Malate/L-lactate dehydrogenases Ecs Escheric... 241 2e-63 mlr9216 Non COG 185 1e-46 >mlr9117 Non COG Length = 383 Score = 617 bits (1592), Expect = e-176, Method: Compositional matrix adjust. Identities = 289/361 (80%), Positives = 333/361 (92%) Query: 1 MVTIKVDNLINFVSEVFSHSESSPEEAKRIATYLTTANLTGHDSHGVIRVPVYVRWKKMG 60 MV IKV+NLI+F++EVF H++SSPEEA+RIATYLTTANLTGHDSHGVIRVP Y+RWKK+G Sbjct: 23 MVIIKVENLIDFIAEVFGHADSSPEEARRIATYLTTANLTGHDSHGVIRVPGYIRWKKIG 82 Query: 61 SIVPNQTPEVVVDTPSLAVVDGKFGYGQTVTPVAVKLGIEKCKNAGLAAVALRNSGHLGR 120 S+VPNQT EV+VDTPSLAV+DGKFGYGQTV PVAVKLGIEKCK AGLAAVALRN+GH+GR Sbjct: 83 SVVPNQTVEVLVDTPSLAVLDGKFGYGQTVAPVAVKLGIEKCKKAGLAAVALRNAGHIGR 142 Query: 121 VGDWAEMAAAEGLVSIHFVTAAGSLLVAPYGGVEKRLSTAPYCVGIPRQGQDPIVLDFAT 180 +GDWAEMAAA+GLVS+HFV A SLLVAP+GGVE+R+STAPYCVGIPRQG DPIVLDFAT Sbjct: 143 IGDWAEMAAADGLVSVHFVNATDSLLVAPFGGVERRMSTAPYCVGIPRQGHDPIVLDFAT 202 Query: 181 SVVAEGKCLVASRGGKKLPIGALVDADGTLSEDPHVLYGPYTPDGPRDHTKGTGAIRAFG 240 SVVAEGKCLVAS GGKKLP GAL+D+DGTLS+DP +LYGPYT D RDHT+G GAIRAFG Sbjct: 203 SVVAEGKCLVASLGGKKLPKGALIDSDGTLSDDPRLLYGPYTSDSTRDHTQGIGAIRAFG 262 Query: 241 DHKGSGLAFICELLGGALTGTGATSSDRRFANGMMAFYIDPKVIDTSNFFDGEITRYTDF 300 +HKGSGLA ICEL+GGALTGTGAT++++RFANGM+AFYIDPKV+D +NFFDGE+TRYTDF Sbjct: 263 EHKGSGLALICELMGGALTGTGATAANQRFANGMLAFYIDPKVVDPANFFDGEVTRYTDF 322 Query: 301 IRATKPIAGTDSVLVPGDPERKTRADRTRSGIPLPDDTWAAIVNTAREVGVSEVSIQRAT 360 IRATKP+ G + V +PGDPER+ RA+RT+ GIPL DDTWAAIVNTARE+GVSE I++AT Sbjct: 323 IRATKPVPGIEQVQLPGDPERRIRAERTKDGIPLLDDTWAAIVNTAREMGVSETDIRQAT 382 Query: 361 S 361 S Sbjct: 383 S 383 >ybiC [C] COG2055 Malate/L-lactate dehydrogenases Eco Escherichia coli K12 Length = 361 Score = 242 bits (617), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 134/357 (37%), Positives = 199/357 (55%), Gaps = 19/357 (5%) Query: 7 DNLINFVSEVFSHSESSPEEAKRIATYLTTANLTGHDSHGVIRVPVYVRWKKMGSIVPNQ 66 L +F+ VF S +EAK +A +L ANL GHDSHG+ +P YVR G + N Sbjct: 10 QTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSWSQGHLQINH 69 Query: 67 TPEVVVDTPSLAVVDGKFGYGQTVTPVAVKLGIEKCKNAGLAAVALRNSGHLGRVGDWAE 126 + V + + +DG +GQ A+ LGIEK G+AAVAL NS H+GR+G WAE Sbjct: 70 HAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHHIGRIGYWAE 129 Query: 127 MAAAEGLVSIHFVTAAGSLLVAPYGGVEKRLSTAPYCVGIPRQGQDPIVLDFATSVVAEG 186 AA G VSIHFV+ G +VAP+ G + R T P+CV PR+ P++LD+ATS +A G Sbjct: 130 QCAAAGFVSIHFVSVVGIPMVAPFHGRDSRFGTNPFCVVFPRKDNFPLLLDYATSAIAFG 189 Query: 187 KCLVASRGGKKLPIGALVDADGTLSEDPHVLYGPYTPDGPRDHTKGTGAIRAFGDHKGSG 246 K VA G +P G L+D +G + +P V+ + P G++ F +HKG Sbjct: 190 KTRVAWHKGVPVPPGCLIDVNGVPTTNPAVMQ-----ESP------LGSLLTFAEHKGYA 238 Query: 247 LAFICELLGGALTGTGAT------SSDRRFANGMMAFYIDPKVIDTSNFFDGEITRYTDF 300 LA +CE+LGGAL+G T +S N M I+P++ + + + + ++ Sbjct: 239 LAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTIIINPELFGAPD-CNAQTEAFAEW 297 Query: 301 IRATKPIAGTDSVLVPGDPERKTRADRTRSGIPLPDDTWAAIVNTAREVGVSEVSIQ 357 ++A+ P +L+PG+ E TR +R + GIPL +W AI + AR++G+ E ++Q Sbjct: 298 VKAS-PHDDDKPILLPGEWEVNTRRERQKQGIPLDAGSWQAICDAARQIGMPEETLQ 353 >ZybiC [C] COG2055 Malate/L-lactate dehydrogenases EcZ Escherichia coli O157:H7 EDL933 Length = 361 Score = 241 bits (616), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 134/357 (37%), Positives = 198/357 (55%), Gaps = 19/357 (5%) Query: 7 DNLINFVSEVFSHSESSPEEAKRIATYLTTANLTGHDSHGVIRVPVYVRWKKMGSIVPNQ 66 L +F+ VF S +EAK +A +L ANL GHDSHG+ +P YVR G + N Sbjct: 10 QTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSWSQGHLQINH 69 Query: 67 TPEVVVDTPSLAVVDGKFGYGQTVTPVAVKLGIEKCKNAGLAAVALRNSGHLGRVGDWAE 126 ++V + + +DG +GQ A+ LGIEK G+AAVAL NS H+GR+G WAE Sbjct: 70 HAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHHIGRIGYWAE 129 Query: 127 MAAAEGLVSIHFVTAAGSLLVAPYGGVEKRLSTAPYCVGIPRQGQDPIVLDFATSVVAEG 186 AA G VSIHFV+ G +VAP+ G + R T P+CV PR+ P++LD+ATS +A G Sbjct: 130 QCAAAGFVSIHFVSVVGIPMVAPFHGRDSRFGTNPFCVVFPRKDDFPLLLDYATSAIAFG 189 Query: 187 KCLVASRGGKKLPIGALVDADGTLSEDPHVLYGPYTPDGPRDHTKGTGAIRAFGDHKGSG 246 K VA G +P G L+D +G + +P V+ + P G++ F +HKG Sbjct: 190 KTRVAWHKGVPVPPGCLIDVNGVPTTNPAVMQ-----ESP------LGSLLTFAEHKGYA 238 Query: 247 LAFICELLGGALTGTGAT------SSDRRFANGMMAFYIDPKVIDTSNFFDGEITRYTDF 300 LA +CE+LGGAL+G T +S N M I+P++ + + + ++ Sbjct: 239 LAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTIIINPELFGAPD-CSAQTEXFAEW 297 Query: 301 IRATKPIAGTDSVLVPGDPERKTRADRTRSGIPLPDDTWAAIVNTAREVGVSEVSIQ 357 ++A+ P +L+PG+ E TR +R GIPL +W AI + AR++G+ E ++Q Sbjct: 298 VKAS-PHDDDKPILLPGEWEVNTRRERQEQGIPLDAGSWQAICDAARQIGMPEETLQ 353 >ECs0879 [C] COG2055 Malate/L-lactate dehydrogenases Ecs Escherichia coli O157:H7 Length = 361 Score = 241 bits (615), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 134/357 (37%), Positives = 198/357 (55%), Gaps = 19/357 (5%) Query: 7 DNLINFVSEVFSHSESSPEEAKRIATYLTTANLTGHDSHGVIRVPVYVRWKKMGSIVPNQ 66 L +F+ VF S +EAK +A +L ANL GHDSHG+ +P YVR G + N Sbjct: 10 QTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSWSQGHLQINH 69 Query: 67 TPEVVVDTPSLAVVDGKFGYGQTVTPVAVKLGIEKCKNAGLAAVALRNSGHLGRVGDWAE 126 ++V + + +DG +GQ A+ LGIEK G+AAVAL NS H+GR+G WAE Sbjct: 70 HAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHHIGRIGYWAE 129 Query: 127 MAAAEGLVSIHFVTAAGSLLVAPYGGVEKRLSTAPYCVGIPRQGQDPIVLDFATSVVAEG 186 AA G VSIHFV+ G +VAP+ G + R T P+CV PR+ P++LD+ATS +A G Sbjct: 130 QCAAAGFVSIHFVSVVGIPMVAPFHGRDSRFGTNPFCVVFPRKDDFPLLLDYATSAIAFG 189 Query: 187 KCLVASRGGKKLPIGALVDADGTLSEDPHVLYGPYTPDGPRDHTKGTGAIRAFGDHKGSG 246 K VA G +P G L+D +G + +P V+ + P G++ F +HKG Sbjct: 190 KTRVAWHKGVPVPPGCLIDVNGVPTTNPAVMQ-----ESP------LGSLLTFAEHKGYA 238 Query: 247 LAFICELLGGALTGTGAT------SSDRRFANGMMAFYIDPKVIDTSNFFDGEITRYTDF 300 LA +CE+LGGAL+G T +S N M I+P++ + + + ++ Sbjct: 239 LAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTIIINPELFGAPD-CSAQTEAFAEW 297 Query: 301 IRATKPIAGTDSVLVPGDPERKTRADRTRSGIPLPDDTWAAIVNTAREVGVSEVSIQ 357 ++A+ P +L+PG+ E TR +R GIPL +W AI + AR++G+ E ++Q Sbjct: 298 VKAS-PHDDDKPILLPGEWEVNTRRERQEQGIPLDAGSWQAICDAARQIGMPEETLQ 353 >mlr9216 Non COG Length = 366 Score = 185 bits (470), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 124/354 (35%), Positives = 179/354 (50%), Gaps = 22/354 (6%) Query: 1 MVTIKVDNLINFVSEVFSHSESSPEEAKRIATYLTTANLTGHDSHGVIRVPVYVRWKKMG 60 + +K D L + + + +S +EA+ +AT ANL GHDSHGVI +P Y+ K G Sbjct: 17 FLVVKSDRLTRICAALLKAAGASEDEAQAVATGCVNANLAGHDSHGVIAIPGYIDSLKAG 76 Query: 61 SIVPNQTPEVVVDTPSLAVVDGKFGYGQTVTPVAVKLGIEKCKNAGLAAVALRNSGHLGR 120 IVP +V ++P+ AV+DG +G+G V A+ L IEK K A +AA + H+GR Sbjct: 77 DIVPGAEWTIVRESPTTAVIDGNWGFGFHVNAKAMALVIEKAKIANVAACTVFRQSHVGR 136 Query: 121 VGDWAEMAAAEGLVSIHFVTAAGSL--LVAPYGGVEKRLSTAPYCVGIPRQGQDPIVLDF 178 + + MA + G++ V +GS VAP+GG E RL T P +G+P Q P +LD Sbjct: 137 LAAYPLMAVSAGMIGFA-VAGSGSFPDCVAPFGGREARLGTNPISIGVPSNLQAPFLLDM 195 Query: 179 ATSVVAEGKCLVASRGGKKLPIGALVDADGTLSEDPHVLYGPYTPDGPRDHTKGTGAIRA 238 ATS A GK VA +K+P G L+DA+G + +PH D+ KG G + Sbjct: 196 ATSAAAAGKLEVAIARREKIPEGWLIDAEGRPTTNPH------------DYLKG-GVLLP 242 Query: 239 FG---DHKGSGLAFICELLGGAL--TGTGATSSDRRFANGMMAFYIDPKVIDTSNFFDGE 293 G +KGSGLA I ELL G L G G S R MA + + N F E Sbjct: 243 LGGPEGYKGSGLAAIVELLCGLLPDPGFGPQPSGRENLGCFMAVF-NVAAFCPLNKFKQE 301 Query: 294 ITRYTDFIRATKPIAGTDSVLVPGDPERKTRADRTRSGIPLPDDTWAAIVNTAR 347 + + ++++ P G+ V PG+ +R +GI + TW + AR Sbjct: 302 VAEFARYLKSATPSEGSPGVFYPGEIGYLRAQERMANGIEIEKATWDKLRTLAR 355