BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0059.0186.p (311 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DR1609 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 152 9e-37 ML1689 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 139 7e-33 MTH1507 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 132 7e-31 mll4780 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 132 9e-31 Rv2993c [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 132 1e-30 >DR1609 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Dra Deinococcus radiodurans Length = 250 Score = 152 bits (384), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 90/241 (37%), Positives = 129/241 (53%), Gaps = 8/241 (3%) Query: 1 MRWLKFTAAGDTSWGLVEGDTVTTVNGDPFGEWNKTSKTHALKDVKIELPLIPRTFYCVG 60 M ++ G WG++EG+T+ G +T +T AL + ++ P P CVG Sbjct: 1 MLIVRVEQRGAAQWGVLEGETIHLTRGL---TGPRTGETLALSEARLLAPAEPSKIVCVG 57 Query: 61 LNYLKHLKEAADKAGTVPNVPDRPEIGYRAQNALIAHDENVVIPAGATEKIHYEGELVVV 120 NYL H++E + G ++P P I + NAL V P+ +E H+EGEL +V Sbjct: 58 RNYLDHIRELGNDTG---DLPAEPGIFLKGPNALAEPGGTVEYPS-WSENFHFEGELALV 113 Query: 121 IGKKARHLTEANAMDCVFGYTIGNDVSERSWQKADRGLWRAKNADTFKPMGPWIETDVDL 180 IG++AR+L A+ V GYT G D++ R QK D +RAK AD F P+GPW+ T D Sbjct: 114 IGQRARNLKADEALGAVLGYTCGLDLTARDRQKTDLQWFRAKAADRFCPLGPWLVTQFDP 173 Query: 181 DRMETAIRVNGKETGRFRTNEMIFGIVPFLVELSKYFTLSPGDVIWMGT-DGASPDLGRD 239 + RVNG+ RT+ MIF +V L L+++ TL PGDV+ GT +G P D Sbjct: 174 SDVRVQTRVNGEPRQDGRTSHMIFDVVQILTYLTRFVTLEPGDVVLTGTPEGVGPLQRGD 233 Query: 240 R 240 R Sbjct: 234 R 234 >ML1689 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Mle Mycobacterium leprae Length = 242 Score = 139 bits (351), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 79/204 (38%), Positives = 114/204 (55%), Gaps = 7/204 (3%) Query: 29 PFGEWNKTSKTHALKDVKIELPLIPRTFYCVGLNYLKHLKEAADKAGTVPNVPDRPEIGY 88 PFGE T ++ L DV++ P++ C+G NY H+ E + G P I Sbjct: 10 PFGELTFTGRSWPLADVRLLAPMLASKVVCIGKNYADHIVEMGGQTGLASA---DPVIFL 66 Query: 89 RAQNALIAHDENVVIPAGATEKIHYEGELVVVIGKKARHLTEANAMDCVFGYTIGNDVSE 148 + A+I + + +PA A+ +H+EGEL +V+G+ + + A+DC+ GYTIGND+S Sbjct: 67 KPNTAIIGPNVPIRLPANAS-PVHFEGELALVVGRSCKDVPAVQAVDCILGYTIGNDMSA 125 Query: 149 RSWQKADRGLWRAKNADTFKPMGPWIETDVD-LDRMETAIR--VNGKETGRFRTNEMIFG 205 R Q+AD RAK DTF P+GPWI TD+ LD + +R VNG+ RT+ MI G Sbjct: 126 RDQQRADGQWTRAKGHDTFCPVGPWIVTDLGPLDPADLELRTAVNGEVKQHSRTSLMIHG 185 Query: 206 IVPFLVELSKYFTLSPGDVIWMGT 229 I + +S TL PGD+I GT Sbjct: 186 IGAIMEWISAVMTLLPGDLILTGT 209 >MTH1507 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Mth Methanothermobacter thermautotrophicus Length = 260 Score = 132 bits (333), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 78/230 (33%), Positives = 121/230 (52%), Gaps = 8/230 (3%) Query: 1 MRWLKFTAAGDTSWGLVEGDTVTTVNGDPFGEWN-KTSKTHALKDVKIELPLIPRTFYCV 59 +++L+F G G E + + + + N + H+L DV+I P+ P CV Sbjct: 4 LKFLRFMKDGRLLHGFTENRRILEIREEEYLSDNPEVIAEHSLDDVRILPPVSPSKVVCV 63 Query: 60 GLNYLKHLKEAADKAGTVPNVPDRPEIGYRAQNALIAHDENVVIPAGATEKIHYEGELVV 119 GLNY H E ++PD P I + +I + V+ P ++E + YE EL Sbjct: 64 GLNYRDHAAELG------MDIPDEPIIFLKPPTTVIGSGDTVIYPEMSSE-VDYEVELAA 116 Query: 120 VIGKKARHLTEANAMDCVFGYTIGNDVSERSWQKADRGLWRAKNADTFKPMGPWIETDVD 179 V+ ++AR + + A + + GYT+ NDV+ R Q+ D RAK+ DTF P+GP IETDV Sbjct: 117 VVSERARRVDSSAASEFIAGYTVLNDVTARDLQRRDGQWTRAKSFDTFCPIGPVIETDVT 176 Query: 180 LDRMETAIRVNGKETGRFRTNEMIFGIVPFLVELSKYFTLSPGDVIWMGT 229 D ++ ++++NG+ T+ MIF + L +S TL PGDVI GT Sbjct: 177 PDNLDISLKLNGEVRQSSNTSNMIFTVHELLEFISHVMTLEPGDVIATGT 226 >mll4780 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Mlo Mesorhizobium loti Length = 281 Score = 132 bits (332), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 72/178 (40%), Positives = 107/178 (60%), Gaps = 11/178 (6%) Query: 56 FYCVGLNYLKHLKEAADKAGTVPNVPDRPEIGYRAQNALIAHDENVVIPAGATEKIHYEG 115 F C+GLNY H E T VP P I +A +A++ D++V+IP G +EK +E Sbjct: 73 FICIGLNYSDHAAE------TGATVPPEPIIFMKASSAIVGPDDDVLIPRG-SEKTDWEV 125 Query: 116 ELVVVIGKKARHLTEANAMDCVFGYTIGNDVSERSWQKADRGLW-RAKNADTFKPMGPWI 174 EL VVIGK A++++EA+A+D V GY + +DVSER++Q +G W + K+ DTF P GPW+ Sbjct: 126 ELAVVIGKTAKYVSEADALDYVAGYAVAHDVSERAFQAERQGQWTKGKSCDTFGPTGPWL 185 Query: 175 ETD---VDLDRMETAIRVNGKETGRFRTNEMIFGIVPFLVELSKYFTLSPGDVIWMGT 229 T D ++ + VNGK T M++G+ + LS++ +L PGD+I GT Sbjct: 186 VTKDEVADPQNLKMWLTVNGKTMQNGSTKTMVYGVKYLVSYLSQFMSLHPGDIISTGT 243 >Rv2993c [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Mtu Mycobacterium tuberculosis H37Rv Length = 239 Score = 132 bits (331), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 78/204 (38%), Positives = 109/204 (53%), Gaps = 10/204 (4%) Query: 29 PFGEWNKTSKTHALKDVKIELPLIPRTFYCVGLNYLKHLKEAADKAGTVPNVPDRPEIGY 88 PFG T ++ L DV++ P++ CVG NY H+ E + P P I Sbjct: 10 PFGTPTFTGRSWPLADVRLLAPILASKVVCVGKNYADHIAEMGGRP------PADPVIFL 63 Query: 89 RAQNALIAHDENVVIPAGATEKIHYEGELVVVIGKKARHLTEANAMDCVFGYTIGNDVSE 148 + A+I + + +PA A+ +H+EGEL +VIG+ + + A A+D + GYTIGNDVS Sbjct: 64 KPNTAIIGPNTPIRLPANAS-PVHFEGELAIVIGRACKDVPAAQAVDNILGYTIGNDVSA 122 Query: 149 RSWQKADRGLWRAKNADTFKPMGPWIETDV---DLDRMETAIRVNGKETGRFRTNEMIFG 205 R Q++D RAK DTF P+GPWI TD+ D +E VNG RT+ MI Sbjct: 123 RDQQQSDGQWTRAKGHDTFCPVGPWIVTDLAPFDPADLELRTVVNGDVKQHARTSLMIHD 182 Query: 206 IVPFLVELSKYFTLSPGDVIWMGT 229 I + +S TL PGD+I GT Sbjct: 183 IGAIVEWISAIMTLLPGDLILTGT 206