BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0059.0164.p (294 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc2623 [R] COG2159 Predicted metal-dependent hydrolase of the T... 437 e-122 Cgl0287 [R] COG2159 Predicted metal-dependent hydrolase of the T... 293 2e-79 SSO2112 [R] COG2159 Predicted metal-dependent hydrolase of the T... 191 2e-48 Rv2303c [R] COG2159 Predicted metal-dependent hydrolase of the T... 94 4e-19 MT2360 [R] COG2159 Predicted metal-dependent hydrolase of the TI... 94 4e-19 >AGc2623 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 291 Score = 437 bits (1125), Expect = e-122, Method: Compositional matrix adjust. Identities = 202/290 (69%), Positives = 244/290 (84%), Gaps = 3/290 (1%) Query: 8 DIEKVTAIDIHTHAEEPCGMHGDDGYDDFQAQMADYFKSPHKHP---PTVPETAAYYRAK 64 +I+++ AID+HTHAEEPC DDGYD+FQA MA YFK+P H PTV ETAAYYR + Sbjct: 2 NIDELIAIDVHTHAEEPCCGPRDDGYDEFQAGMAKYFKNPAGHKGMLPTVQETAAYYRER 61 Query: 65 NIAAVIFPVDAERETGSRRYNNYEMLEVASDHLDVLIPFVSIDPHKGKLGVREARKLIEE 124 I VIFPVDAERETG RRY N E+ ++A+++ D++IPF SIDP KGK G REAR+L+ E Sbjct: 62 KIGCVIFPVDAERETGFRRYENEEVAKIAAENSDIMIPFASIDPAKGKAGAREARRLVRE 121 Query: 125 HGVRGFKFHPTMQGFYANDRMAYPLYEAINDGGAIALFHTGQTGVGSGMPGGMGMRLKYS 184 GV+GFKFHPTMQGFY NDRMAYPLYEAI + GAI LFHTGQTGVG+G+ GGM MRLK+S Sbjct: 122 FGVKGFKFHPTMQGFYPNDRMAYPLYEAIAEEGAITLFHTGQTGVGAGLRGGMAMRLKFS 181 Query: 185 NPMYMDDVAADFPDLKIILAHPSFPWQEEALSVATHKPNVYIDLSGWSPKYFPPILVRYI 244 NP+++DDVA DFPD+ IILAHPSFPWQEEALSVATHKPNVYID+SGWSPKYFPPILV+Y Sbjct: 182 NPIHLDDVAVDFPDMPIILAHPSFPWQEEALSVATHKPNVYIDMSGWSPKYFPPILVQYA 241 Query: 245 NSILQDKMLFGSDWPVITPDRWLADFAKLDIREEIRPKVMKANARKILGI 294 NS+L+ KMLFGSDWP +TP+RWL DFA + I++E+RP ++K NAR++L + Sbjct: 242 NSLLKHKMLFGSDWPAMTPERWLNDFANISIKDEVRPLILKENARRLLKL 291 >Cgl0287 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Cgl Corynebacterium glutamicum Length = 314 Score = 293 bits (751), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 141/288 (48%), Positives = 200/288 (69%), Gaps = 4/288 (1%) Query: 8 DIEKVTAIDIHTHAE-EPCGMHGDDGYDDFQAQMADYFKSPHKHPPTVPETAAYYRAKNI 66 D + + A+D+H H E + CG D A + YFK+ + P+ A YR + Sbjct: 13 DADNIVAVDMHVHLEVDSCGHKSMPA--DIMAASSKYFKTAER-TPSADAIADIYREHKM 69 Query: 67 AAVIFPVDAERETGSRRYNNYEMLEVASDHLDVLIPFVSIDPHKGKLGVREARKLIEEHG 126 AAV+F +DA + G + +++ + + DVLIPF S+DP G+ + EAR+ +EE G Sbjct: 70 AAVVFTIDARTQMGHLPNSIDDLVASCARNNDVLIPFGSVDPRTGEDALVEARRQVEELG 129 Query: 127 VRGFKFHPTMQGFYANDRMAYPLYEAINDGGAIALFHTGQTGVGSGMPGGMGMRLKYSNP 186 VRGFKFHP++QGF + YPL+E + G +FHTGQ G+G+G+PGG G++L++SNP Sbjct: 130 VRGFKFHPSVQGFDPSAPEFYPLWELLESFGLPCVFHTGQNGMGAGLPGGRGIKLRFSNP 189 Query: 187 MYMDDVAADFPDLKIILAHPSFPWQEEALSVATHKPNVYIDLSGWSPKYFPPILVRYINS 246 M +DDVAADFP+L II+AHPS PWQ+EA S+ATHK NV+IDLSGWSPKYFP LVR N+ Sbjct: 190 MLLDDVAADFPNLTIIMAHPSVPWQDEANSIATHKANVFIDLSGWSPKYFPESLVRQSNN 249 Query: 247 ILQDKMLFGSDWPVITPDRWLADFAKLDIREEIRPKVMKANARKILGI 294 +L K+LFG+D+P+ITP++WLA FA L +++E+RP ++K NA K+LG+ Sbjct: 250 VLSKKVLFGTDFPLITPEKWLAAFANLPLKDEVRPGILKDNAVKVLGL 297 >SSO2112 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Sso Sulfolobus solfataricus Length = 269 Score = 191 bits (485), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 106/279 (37%), Positives = 162/279 (58%), Gaps = 15/279 (5%) Query: 15 IDIHTHAEEPCGMHGDDGYDDFQAQMADYFKSPHKHPPTVPETAAYYRAKNIAA-VIFPV 73 ID H HA + D +F YF + + ET YY + I V+ P+ Sbjct: 2 IDFHFHAPVKEFL---DFLGEFGEPAIKYFNA-KVEVKGLKETLDYYESFGIKRFVVLPI 57 Query: 74 DAERETGSRRYNNYEMLEVASDHLDVLIPFVSIDPHKGKLGVREARKLIEEHGVRGFKFH 133 D+ G R N L+ D ++ F+S+DP K E ++ I+E G K H Sbjct: 58 DSTTFLGRRIPNQVINLD------DRIVKFISVDPSKPNAD-EELKRSIKEFEPVGVKLH 110 Query: 134 PTMQGFYANDRMAYPLYEAINDGGAIALFHTGQTGVGSGMPGGMGMRLKYSNPMYMDDVA 193 P +QGF+ D A +YE I++ G I +FHTG +G+G+G+ + RL Y P+Y D++A Sbjct: 111 PQLQGFHPLDERALKIYEIIDNHGLIVVFHTGTSGIGAGVKSSI--RLDYGRPIYFDEIA 168 Query: 194 ADFPDLKIILAHPSFPWQEEALSVATHKPNVYIDLSGWSPKYFPPILVRYINSILQDKML 253 F ++KIILAH +PW EEA+++A HKPNVY+DLSGW+P+Y P ++ L DK+L Sbjct: 169 VRFQNMKIILAHFGWPWTEEAIAIALHKPNVYLDLSGWAPRYIPQVIWSNAKR-LSDKLL 227 Query: 254 FGSDWPVITPDRWLADFAKLDIREEIRPKVMKANARKIL 292 FGSD+P+I P+RWL +F +++ ++I+ K++K NA K++ Sbjct: 228 FGSDFPLIRPERWLEEFKSVNLSQDIKDKILKHNAEKLV 266 >Rv2303c [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Mtu Mycobacterium tuberculosis H37Rv Length = 307 Score = 94.0 bits (232), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 73/286 (25%), Positives = 122/286 (42%), Gaps = 30/286 (10%) Query: 30 DDGYDDFQAQMADYFKSPHKHPPTVPETAAYYR---------------AKNIAAVIFPVD 74 DD + F ++ YF+ K + + AY A ++I D Sbjct: 25 DDLREGFPVELLSYFEVFTKTTISAEQFGAYAESLRRTDDQILDSLDDAGITRSLITGFD 84 Query: 75 AERETGSRRYNNYEMLEVASDHLDVLIPFVSIDPHKGKLGVREARKLIEEHGVRGFKFHP 134 G +N + VA+ + D +PF D G V E + + EHG RG P Sbjct: 85 ERSTCGVTFVHNASVAAVAARYPDRFLPFAGADILAGDSAVDEFERWVVEHGFRGLSLRP 144 Query: 135 TMQGFYANDRMAYPLYEAINDGGAIALFHTGQTGVGSGMPGGMGMRLKYSNPMYMDDVAA 194 M G A+D +P Y + G HT + + +P ++DDVA Sbjct: 145 FMIGRPASDPAYFPCYAKCVELGVPVSIHTSADWTRTRLS-------DLGHPRHIDDVAC 197 Query: 195 DFPDLKIILAHPSFPWQEEALSVATHKPNVYIDLSGWSPKYFPP------ILVRYINSIL 248 FP+L I+++H +PW +A +A PNVY++L+ PKYF L+R+ + + Sbjct: 198 RFPELTILMSHGGYPWVLQACLIAWKHPNVYLELAAHRPKYFASPGAGWEPLMRFGQTTI 257 Query: 249 QDKMLFGSDWPVIT-PDRWLAD-FAKLDIREEIRPKVMKANARKIL 292 ++K+++G+ +I P L D L + E+ + NA ++L Sbjct: 258 RNKIVYGTGGFLINRPYLQLCDEMRALPVPREVLEDWLWRNATRVL 303 >MT2360 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold MtC Mycobacterium tuberculosis CDC1551 Length = 307 Score = 94.0 bits (232), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 73/286 (25%), Positives = 122/286 (42%), Gaps = 30/286 (10%) Query: 30 DDGYDDFQAQMADYFKSPHKHPPTVPETAAYYR---------------AKNIAAVIFPVD 74 DD + F ++ YF+ K + + AY A ++I D Sbjct: 25 DDLREGFPVELLSYFEVFTKTTISAEQFGAYAESLRRTDDQILDSLDDAGITRSLITGFD 84 Query: 75 AERETGSRRYNNYEMLEVASDHLDVLIPFVSIDPHKGKLGVREARKLIEEHGVRGFKFHP 134 G +N + VA+ + D +PF D G V E + + EHG RG P Sbjct: 85 ERSTCGVTFVHNASVAAVAARYPDRFLPFAGADILAGDSAVDEFERWVVEHGFRGLSLRP 144 Query: 135 TMQGFYANDRMAYPLYEAINDGGAIALFHTGQTGVGSGMPGGMGMRLKYSNPMYMDDVAA 194 M G A+D +P Y + G HT + + +P ++DDVA Sbjct: 145 FMIGRPASDPAYFPCYAKCVELGVPVSIHTSADWTRTRLS-------DLGHPRHIDDVAC 197 Query: 195 DFPDLKIILAHPSFPWQEEALSVATHKPNVYIDLSGWSPKYFPP------ILVRYINSIL 248 FP+L I+++H +PW +A +A PNVY++L+ PKYF L+R+ + + Sbjct: 198 RFPELTILMSHGGYPWVLQACLIAWKHPNVYLELAAHRPKYFASPGAGWEPLMRFGQTTI 257 Query: 249 QDKMLFGSDWPVIT-PDRWLAD-FAKLDIREEIRPKVMKANARKIL 292 ++K+++G+ +I P L D L + E+ + NA ++L Sbjct: 258 RNKIVYGTGGFLINRPYLQLCDEMRALPVPREVLEDWLWRNATRVL 303