BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0059.0132.p (335 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF0939 [R] COG1234 Metal-dependent hydrolases of the beta-lactam... 189 1e-47 BS_yqjK [R] COG1234 Metal-dependent hydrolases of the beta-lacta... 177 4e-44 MA3031 [R] COG1234 Metal-dependent hydrolases of the beta-lactam... 166 6e-41 MTH1831 [R] COG1234 Metal-dependent hydrolases of the beta-lacta... 164 4e-40 lin2084 [R] COG1234 Metal-dependent hydrolases of the beta-lacta... 158 2e-38 >AF0939 [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Afu Archaeoglobus fulgidus Length = 306 Score = 189 bits (479), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 114/301 (37%), Positives = 169/301 (56%), Gaps = 11/301 (3%) Query: 35 MFSLTFLGTSASVPSPERNHPGILVEAAGSRILVDCGEGTQRQLLRSGAGFRRLDRLLLT 94 +F +TFLGTS ++PS ERN P I V+ G R+L DCGEGTQRQ++ + GFR LD + +T Sbjct: 2 LFKITFLGTSGTIPSVERNSPAIFVQFGGQRMLFDCGEGTQRQMMIAKTGFRNLDNIFIT 61 Query: 95 HGHFDHVLGIPGLFSTLRLRQSSDAMTIHAGPGTLDVVVRMLAGLWGEGRAPIPLDLVPL 154 H H DH +G+ GL T+ L + S + +++ V+R L +G + + + L Sbjct: 62 HLHTDHFIGLFGLIETMSLNERSRELNVYSPRAE---VLRALFEAFGYDQLNYDIRVREL 118 Query: 155 SEGRVLDAGEFTIDCFAVRHRDTDSFAFSFQSAMRRHLLPDRLLA---LRVPDGPVRREL 211 +G + F + F H S ++ RR +R A L +P GP+ +L Sbjct: 119 KDGEEVKFDGFKVVAFRTEH-IVKSVGYAIIENDRRGKF-NREKAEKELGIPPGPLYAKL 176 Query: 212 AEGRQVKLDDGRTIDPEQVLGSAEGRKKLVIVGDAETTEGLAELVRDADVLVIEATFLER 271 A G + + GRTI P+ VLG +K+V GD+ T+ E+ R+AD+L+ +A+F E Sbjct: 177 ARGESI-VWKGRTITPDMVLGEKRRGRKVVYTGDSRPTKRTVEIARNADILIHDASFKEE 235 Query: 272 DAAIARDYGHLTASEAAALAARSGVKQLILTHISGRYPDE--DILAEATRIFPNSRIAID 329 A + GH TA EAA +A + VK+LILTHIS RY + +L EA ++F N+ IA D Sbjct: 236 LKDWAIESGHSTAKEAAEVAREANVKKLILTHISTRYSKDASPLLEEAKKVFENTIIAED 295 Query: 330 F 330 F Sbjct: 296 F 296 >BS_yqjK [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Bsu Bacillus subtilis Length = 307 Score = 177 bits (448), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 107/307 (34%), Positives = 160/307 (52%), Gaps = 10/307 (3%) Query: 36 FSLTFLGTSASVPSPERNHPGI---LVEAAGSRILVDCGEGTQRQLLRSGAGFRRLDRLL 92 L FLGT A +P+ RN + L+E S L DCGE TQ Q+L + R+++++ Sbjct: 1 MELLFLGTGAGIPAKARNVTSVALKLLEERRSVWLFDCGEATQHQILHTTIKPRKIEKIF 60 Query: 93 LTHGHFDHVLGIPGLFSTLRLRQSSDAMTIHAGPGTLDVVVRMLAGLWGEGRAPIPLDLV 152 +TH H DHV G+PGL + + D +T++ G + LA + PL + Sbjct: 61 ITHMHGDHVYGLPGLLGSRSFQGGEDELTVYGPKGIKAFIETSLA--VTKTHLTYPLAIQ 118 Query: 153 PLSEGRVLDAGEFTIDCFAVRHRDTDSFAFSFQSA-MRRHLLPDRLLALRVPDGPVRREL 211 + EG V + +F + +V H ++F + Q + L D L + +P GPV +++ Sbjct: 119 EIEEGIVFEDDQFIVTAVSVIH-GVEAFGYRVQEKDVPGSLKADVLKEMNIPPGPVYQKI 177 Query: 212 AEGRQVKLDDGRTIDPEQVLGSAEGRKKLVIVGDAETTEGLAELVRDADVLVIEATFLER 271 +G V L+DGR I+ L + + +V GD ++ L EL RD DVLV EATF + Sbjct: 178 KKGETVTLEDGRIINGNDFLEPPKKGRSVVFSGDTRVSDKLKELARDCDVLVHEATFAKE 237 Query: 272 DAAIARDYGHLTASEAAALAARSGVKQLILTHISGRYPDE---DILAEATRIFPNSRIAI 328 D +A DY H T +AA A + KQLILTHIS RY + ++ EA +FPNS A Sbjct: 238 DRKLAYDYYHSTTEQAAVTAKEARAKQLILTHISARYQGDASLELQKEAVDVFPNSVAAY 297 Query: 329 DFDRIAI 335 DF + + Sbjct: 298 DFLEVNV 304 >MA3031 [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Mac Methanosarcina acetivorans str.C2A Length = 305 Score = 166 bits (420), Expect = 6e-41, Method: Compositional matrix adjust. Identities = 104/308 (33%), Positives = 161/308 (52%), Gaps = 15/308 (4%) Query: 35 MFSLTFLGTSASVPSPERNHPGILVEAAGSRILVDCGEGTQRQLLRSGAGFRRLDRLLLT 94 M +TFLGT S+P+ RN +++ G +L DCGEGTQ+Q++R+ G L + ++ Sbjct: 1 MLRITFLGTGGSLPTRNRNPSAVMINREGELMLFDCGEGTQQQMMRAKTGMMSLSSIFVS 60 Query: 95 HGHFDHVLGIPGLFSTLRLRQSSDAMTIHAGPGTLDVVVRMLAGLWGEGRAPIPLDLVPL 154 H H DH LGIPGL T+ D + I+ GT + A G + + L Sbjct: 61 HFHADHFLGIPGLIQTMSFMGRKDPLMIYGPAGTREFTELFKA--LGYFNLKYEIHGMEL 118 Query: 155 SEGRVLDAGEFTIDCFAVRHRDTDSFAFSFQSAMRRHLLPDRL-----LALRVPDGPVRR 209 G V++ + + VR +T+ S A+ + P R +AL VP GP+ Sbjct: 119 KPGDVVEG-----EGYVVRALETEHSTPSLGYALIENPRPGRFNREKAVALGVPPGPLFS 173 Query: 210 ELAEGRQVKLDDGRTIDPEQVLGSAEGRKKLVIVGDAETTEGLAELVRDADVLVIEATFL 269 +L +G V+ G+ + PE+V+G+ + +V GD E + E RDAD+L+ + +F Sbjct: 174 KLQKGNPVEA-GGKVVRPEEVMGTPRPGRTIVYSGDTRPCEAVLEASRDADLLIHDGSFA 232 Query: 270 ERDAAIARDYGHLTASEAAALAARSGVKQLILTHISGRYPD--EDILAEATRIFPNSRIA 327 + A A + H TA E AALA +GV++L+LTHIS RY D E IL ++ ++F N +A Sbjct: 233 DEMAEWAEESMHSTAGEVAALAKEAGVRKLVLTHISSRYTDDVEPILKDSKKVFENVIVA 292 Query: 328 IDFDRIAI 335 D + I Sbjct: 293 EDLMELEI 300 >MTH1831 [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Mth Methanothermobacter thermautotrophicus Length = 307 Score = 164 bits (414), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 101/302 (33%), Positives = 162/302 (53%), Gaps = 5/302 (1%) Query: 35 MFSLTFLGTSASVPSPERNHPGILVEAAGSRILVDCGEGTQRQLLRSGAGFRRLDRLLLT 94 M +TFLGTS++VPS RNH I + G L DCGEGTQRQ+ +G ++ R+ +T Sbjct: 1 MMEVTFLGTSSAVPSKNRNHTSIALRIPGEIFLFDCGEGTQRQMALAGISPMKVTRIFIT 60 Query: 95 HGHFDHVLGIPGLFSTLRLRQSSDAMTIHAGPGTLDVVVRMLAGLWGEGRAPIPLDLVPL 154 H H DH+LGIPG+ ++ R + + I+ PG ++ ++ G +++ + Sbjct: 61 HLHGDHILGIPGMIQSMGFRGREEPLDIYGPPGIHELHECIMK--MGYFTLDFDINVHEV 118 Query: 155 SEGRVLDAGEFTIDCFAVRHRDTDSFAFSFQSAMRRHLLPDRLLALRVPDGPVRRELAEG 214 G V++ ++ + H + A+ F+ R L ++ +AL + GP +L G Sbjct: 119 RGGTVVEEDDYRVTSAPASH-SVFNLAYCFEEKKRPRFLREKAIALGLKPGPAFGKLHRG 177 Query: 215 RQVKLDDGRTIDPEQVLGSAEGRKKLVIVGDAETTEGLAELVRDADVLVIEATFLERDAA 274 V++ D R I PE+VLGS K+ GD E + +L A++L+ E+T Sbjct: 178 IPVRVGD-RIIMPEEVLGSPRKGVKVCYSGDTRPCESVIKLAEGAELLIHESTLEAGSED 236 Query: 275 IARDYGHLTASEAAALAARSGVKQLILTHISGRYPDEDILAEATR-IFPNSRIAIDFDRI 333 A + GH TA EAA +A +GVK+LILTH+S RY +++ EA R +FP + +A D + Sbjct: 237 KAAESGHSTAREAAEVARSAGVKRLILTHLSTRYKRTEVILEAARQVFPVTDVADDLMTV 296 Query: 334 AI 335 + Sbjct: 297 EV 298 >lin2084 [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Lin Listeria innocua Length = 306 Score = 158 bits (399), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 111/304 (36%), Positives = 154/304 (50%), Gaps = 18/304 (5%) Query: 38 LTFLGTSASVPSPERNHPGILVEAAGSR---ILVDCGEGTQRQLLRSGAGFRRLDRLLLT 94 L FLGT A VPS RN I + R L DCGE TQ Q+LRS +L+++ +T Sbjct: 3 LVFLGTGAGVPSRGRNVTSIALSMLNERNAIWLFDCGEATQHQVLRSHIKLSKLEKIFIT 62 Query: 95 HGHFDHVLGIPGLFSTLRLRQSSDAMTIHAGPGTLDVVVRMLAGLWGEGRAPIPLDLVPL 154 H H DH+ G+PGL S+ + +TI+ GP + V L G R ++ + Sbjct: 63 HMHGDHIFGLPGLLSSRSFQGGDSDLTIY-GPAGIAEYVETSLRLSGT-RLTYKINFEEI 120 Query: 155 SEGRVLDAGEFTIDCFAVRHRDTD----SFAFSFQSAMRRHLL-PDRLLALRVPDGPVRR 209 G + F + F V D D SF + ++ L ++L A V GPV + Sbjct: 121 EPGVI-----FEDEMFLVTADDLDHGVRSFGYRIVEKDKQGALNAEKLKADGVEAGPVFQ 175 Query: 210 ELAEGRQVKLDDGRTIDPEQVLGSAEGRKKLVIVGDAETTEGLAELVRDADVLVIEATFL 269 +L G V L DGR ID + +G + K + I GD + T EL +AD+LV EATF Sbjct: 176 KLKNGEIVTLADGRVIDGKNYIGEPQKGKIISIFGDTKETVSELELAMNADILVHEATFE 235 Query: 270 ERDAAIARDYGHLTASEAAALAARSGVKQLILTHISGRY---PDEDILAEATRIFPNSRI 326 A +A +Y H T +AA LA + VK+LILTHIS RY +++L EA +F N+ I Sbjct: 236 GDKAKMAGEYMHSTTLQAANLAKAANVKKLILTHISSRYDRDASKELLIEAKTVFENTEI 295 Query: 327 AIDF 330 A D Sbjct: 296 AYDL 299