BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0059.0099.p (400 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEI0884 [L] COG0188 Type IIA topoisomerase (DNA gyrase/topo II,... 518 e-146 SMc01231 [L] COG0188 Type IIA topoisomerase (DNA gyrase/topo II,... 509 e-144 AGc2778 [L] COG0188 Type IIA topoisomerase (DNA gyrase/topo II, ... 502 e-142 mll0732 [L] COG0188 Type IIA topoisomerase (DNA gyrase/topo II, ... 489 e-138 CC1580 [L] COG0188 Type IIA topoisomerase (DNA gyrase/topo II, t... 466 e-131 >BMEI0884 [L] COG0188 Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit Bme Brucella melitensis Length = 902 Score = 518 bits (1333), Expect = e-146, Method: Compositional matrix adjust. Identities = 262/384 (68%), Positives = 308/384 (80%), Gaps = 9/384 (2%) Query: 1 VKQQFATPRKTEIIEQEGEVEDEDLIQREDMVVTVSHAGYVKRVPLSTYRAQRRGGKGRA 60 V+ +FATPR+TEI E++DEDLI REDMVVTVSHAGY+KRVPL+TYRAQRRGGKGR+ Sbjct: 484 VRDEFATPRRTEIGFGGAEMDDEDLIAREDMVVTVSHAGYIKRVPLTTYRAQRRGGKGRS 543 Query: 61 GMQTRDEDFVSRLFVASTHTPVLFFSSRGQVYKEKVWRLPMAAPNARGKALINILPLEQG 120 GM T++EDFV+RLFVA+THTPVLFFSSRG VYKEK+WRLP+ P +RGKALIN+LPL+QG Sbjct: 544 GMATKEEDFVTRLFVANTHTPVLFFSSRGIVYKEKLWRLPVGTPQSRGKALINMLPLQQG 603 Query: 121 ERITTIMPLPEDESSWANLDVMFATTGGTVRRNKLSDFVDVRRSGIIAMKL-GEGEAIVD 179 ERITTIMPLPEDE SWANLDVMF+TT GTVRRNKLSDFV V R+G IAMKL EG+ I+ Sbjct: 604 ERITTIMPLPEDEDSWANLDVMFSTTRGTVRRNKLSDFVQVNRNGKIAMKLEDEGDEILS 663 Query: 180 VQICTERDDVLLTAAGGQCIRFPVTDVRVFTGRTSMGVRGIALAEADKLISLAILRHVET 239 V CTE DDVLLT AGGQCIRFPVTDVRVF GR S+GVRGI LAE DK+IS+AIL HV+ Sbjct: 664 VDTCTEFDDVLLTCAGGQCIRFPVTDVRVFAGRNSIGVRGINLAEGDKVISMAILHHVDA 723 Query: 240 TSDERSAYLKM----RRAVAGEAAAEEPVEAEGEETANAIQLSQERYAELSAAEQVVLTV 295 T ERSAYLK RRA + A + + GE+ +LS++RY EL A E+ VLTV Sbjct: 724 TPAERSAYLKRSIAERRAQGADDADD--IVVVGEDVDAEAELSEDRYQELKAREETVLTV 781 Query: 296 SVNGYGKRTSSYEYRTTGRGGKGIVAMSVNNRN--GNLVASFPVEDADQIMLVTDKGQLI 353 S GYGKR+SSYE+R +GRGGKGI A + + G LVA FPVE +DQI+LV+D GQLI Sbjct: 782 SEFGYGKRSSSYEFRISGRGGKGIRATDTSKTDEIGKLVALFPVEGSDQILLVSDGGQLI 841 Query: 354 RCPVADIRVAGRSTQGVIVFDTAE 377 R PV IR+AGRST+GV +F+TA+ Sbjct: 842 RVPVDGIRIAGRSTKGVTIFNTAD 865 >SMc01231 [L] COG0188 Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit Sme Sinorhizobium meliloti Length = 931 Score = 509 bits (1312), Expect = e-144, Method: Compositional matrix adjust. Identities = 255/385 (66%), Positives = 305/385 (79%), Gaps = 10/385 (2%) Query: 1 VKQQFATPRKTEIIEQEGEVEDEDLIQREDMVVTVSHAGYVKRVPLSTYRAQRRGGKGRA 60 V+ +F TPR+TEI E +++DEDLI +EDMVVTVSH GY+KRVPL+TYRAQRRGGKGR+ Sbjct: 508 VRDEFGTPRRTEIAEGGPDMDDEDLIAQEDMVVTVSHLGYIKRVPLTTYRAQRRGGKGRS 567 Query: 61 GMQTRDEDFVSRLFVASTHTPVLFFSSRGQVYKEKVWRLPMAAPNARGKALINILPLEQG 120 GM TRDEDFV+RLFVA+THTPVLFFSSRG VYKEKVWRLP+ P +RGKALIN+LPLE G Sbjct: 568 GMATRDEDFVTRLFVANTHTPVLFFSSRGIVYKEKVWRLPIGTPQSRGKALINMLPLEPG 627 Query: 121 ERITTIMPLPEDESSWANLDVMFATTGGTVRRNKLSDFVDVRRSGIIAMKL-GEGEAIVD 179 ERITTIMPLPEDE++W NLDVMF+TT GTVRRNKLSDFV V R+G IAMKL EG+ I+ Sbjct: 628 ERITTIMPLPEDETTWENLDVMFSTTRGTVRRNKLSDFVQVNRNGKIAMKLEEEGDEILS 687 Query: 180 VQICTERDDVLLTAAGGQCIRFPVTDVRVFTGRTSMGVRGIALAEADKLISLAILRHVET 239 V CTE DDV+LT A GQCIRFPV DVRVF GR S+GVRGI+L + D++IS+AI+ HVE Sbjct: 688 VDTCTEFDDVVLTTALGQCIRFPVADVRVFAGRNSIGVRGISLGDGDRIISMAIVAHVEA 747 Query: 240 TSDERSAYLKM----RRAVAGEAAAEEPVEAEGEETANAIQLSQERYAELSAAEQVVLTV 295 ER+AYLK RRA+ GE EE + GEE N +L+ ERY EL A EQ +LTV Sbjct: 748 EPWERAAYLKRSAAERRALTGE---EEEIVLVGEEVTNGGELTNERYEELKAREQFILTV 804 Query: 296 SVNGYGKRTSSYEYRTTGRGGKGIVAMSVNNRN--GNLVASFPVEDADQIMLVTDKGQLI 353 S+ G+GKR+SSY++RT+GRGGKGI A + G LVA+FP+E DQIMLV+D GQLI Sbjct: 805 SMKGFGKRSSSYDFRTSGRGGKGIRATDTSKTAEIGELVAAFPIEHNDQIMLVSDGGQLI 864 Query: 354 RCPVADIRVAGRSTQGVIVFDTAED 378 R PV IR+A R+T+GV +F TA+D Sbjct: 865 RVPVEGIRLASRATKGVTIFSTAKD 889 >AGc2778 [L] COG0188 Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 930 Score = 502 bits (1292), Expect = e-142, Method: Compositional matrix adjust. Identities = 253/383 (66%), Positives = 304/383 (79%), Gaps = 6/383 (1%) Query: 1 VKQQFATPRKTEIIEQEGEVEDEDLIQREDMVVTVSHAGYVKRVPLSTYRAQRRGGKGRA 60 ++ +F TPR+++I+E +++DEDLI REDMVVTVSH GY+KRVPL+TYRAQRRGGKGR+ Sbjct: 508 IRDEFGTPRRSQIVEGGPDMDDEDLIAREDMVVTVSHLGYIKRVPLTTYRAQRRGGKGRS 567 Query: 61 GMQTRDEDFVSRLFVASTHTPVLFFSSRGQVYKEKVWRLPMAAPNARGKALINILPLEQG 120 GM TRDEDFV+RLFVA+THTPVLFFSSRG VYKEKVWRLP+ P ++GKALIN+LPLE G Sbjct: 568 GMATRDEDFVNRLFVANTHTPVLFFSSRGIVYKEKVWRLPIGTPQSKGKALINMLPLEPG 627 Query: 121 ERITTIMPLPEDESSWANLDVMFATTGGTVRRNKLSDFVDVRRSGIIAMKLG-EGEAIVD 179 ERITTIMPLPEDE++W LDVMF+TT GTVRRNKL DFV V R+G IAMKL EG+ I+ Sbjct: 628 ERITTIMPLPEDETAWETLDVMFSTTRGTVRRNKLGDFVQVNRNGKIAMKLDEEGDEILS 687 Query: 180 VQICTERDDVLLTAAGGQCIRFPVTDVRVFTGRTSMGVRGIALAEADKLISLAILRHVET 239 V+ CT+RDDVLLT A GQCIRFPV DVRVF GR S+GVRGI +AE D++IS+ I+ HVE Sbjct: 688 VETCTDRDDVLLTTALGQCIRFPVDDVRVFAGRNSVGVRGINMAEGDRIISMTIVGHVEA 747 Query: 240 TSDERSAYLKMRRAVAGEAAA--EEPVEAEGEETANAIQLSQERYAELSAAEQVVLTVSV 297 ER+AYLK R A AA EE + GEE +LS+ERY EL A E+ VLTVSV Sbjct: 748 EPWERAAYLK-RSATERRAAGVDEEDIALVGEEVTEEGELSEERYQELKAREEFVLTVSV 806 Query: 298 NGYGKRTSSYEYRTTGRGGKGIVAMSVNNRN--GNLVASFPVEDADQIMLVTDKGQLIRC 355 G+GKR+SSY++RT+GRGGKGI A N G LVA+FPVE+ DQIMLV+D GQLIR Sbjct: 807 KGFGKRSSSYDFRTSGRGGKGIRATDTAKTNEIGELVAAFPVEEGDQIMLVSDGGQLIRV 866 Query: 356 PVADIRVAGRSTQGVIVFDTAED 378 PV IR+A R+T+GV +F TA+D Sbjct: 867 PVNGIRIASRATKGVTIFSTAKD 889 >mll0732 [L] COG0188 Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit Mlo Mesorhizobium loti Length = 906 Score = 489 bits (1258), Expect = e-138, Method: Compositional matrix adjust. Identities = 247/384 (64%), Positives = 298/384 (77%), Gaps = 7/384 (1%) Query: 1 VKQQFATPRKTEIIEQEGEVEDEDLIQREDMVVTVSHAGYVKRVPLSTYRAQRRGGKGRA 60 V+ +F TPR+TE+ + ++EDEDLIQREDMVVTVSH+GY+KRVPLS YRAQRRGGKGR+ Sbjct: 484 VRDEFGTPRRTELTDGGADMEDEDLIQREDMVVTVSHSGYIKRVPLSLYRAQRRGGKGRS 543 Query: 61 GMQTRDEDFVSRLFVASTHTPVLFFSSRGQVYKEKVWRLPMAAPNARGKALINILPLEQG 120 GM T++EDFV+RLFVA+THTPVLFFSSRG VYKEKVWRLP+ P +RGKALIN+LPLEQG Sbjct: 544 GMSTKEEDFVTRLFVANTHTPVLFFSSRGIVYKEKVWRLPIGNPQSRGKALINMLPLEQG 603 Query: 121 ERITTIMPLPEDESSWANLDVMFATTGGTVRRNKLSDFVDVRRSGIIAMKL-GEGEAIVD 179 ERITTIMPLPEDE+SW LDVMFATT GTVRRNKLSDFV V R+G IAMKL EG+ I+ Sbjct: 604 ERITTIMPLPEDETSWGELDVMFATTRGTVRRNKLSDFVQVNRNGKIAMKLEEEGDEILG 663 Query: 180 VQICTERDDVLLTAAGGQCIRFPVTDVRVFTGRTSMGVRGIALAEADKLISLAILRHVET 239 V+ CT+ DDVLLTA+ GQCIRF V DVRVF R S+GVRGI +AE D++IS++++ +V+ Sbjct: 664 VETCTDNDDVLLTASSGQCIRFSVGDVRVFQSRNSVGVRGITMAETDRIISMSVIENVDA 723 Query: 240 TSDERSAYLKMRRAVAGEAAA----EEPVEAEGEETANAIQLSQERYAELSAAEQVVLTV 295 + ER+AYLK A AA EE + EE +LS ERY L A EQ VLTV Sbjct: 724 SPAERAAYLKRAAAERRLAAGANGEEEEIALTNEEIGEEAELSDERYEFLKAHEQYVLTV 783 Query: 296 SVNGYGKRTSSYEYRTTGRGGKGIVAMSVNNRN--GNLVASFPVEDADQIMLVTDKGQLI 353 + GYGKR+SSY++R TGRGGKGI A V+ G LVA+FPV + DQIMLV+D G +I Sbjct: 784 TEYGYGKRSSSYDFRLTGRGGKGIRATDVSKTAEIGRLVATFPVGNDDQIMLVSDGGTVI 843 Query: 354 RCPVADIRVAGRSTQGVIVFDTAE 377 R PV IR A R+T+GV +F+TAE Sbjct: 844 RVPVNGIRFASRATKGVTIFNTAE 867 >CC1580 [L] COG0188 Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit Ccr Caulobacter vibrioides Length = 919 Score = 466 bits (1198), Expect = e-131, Method: Compositional matrix adjust. Identities = 221/383 (57%), Positives = 293/383 (76%), Gaps = 5/383 (1%) Query: 1 VKQQFATPRKTEIIEQEGEVEDEDLIQREDMVVTVSHAGYVKRVPLSTYRAQRRGGKGRA 60 V+++FA PR+ +I++ + ++EDEDLI REDMV+TV+ GYVKR PL+ YR Q RGGKG++ Sbjct: 509 VREKFAIPRRCQIVDGDADMEDEDLIVREDMVITVTMGGYVKRTPLANYRTQHRGGKGKS 568 Query: 61 GMQTRDEDFVSRLFVASTHTPVLFFSSRGQVYKEKVWRLPMAAPNARGKALINILPLEQG 120 GM T++ED V+R+F ASTH P+LFF+S G+VYK KVWRLP+ N+RGKA +N+LP+E G Sbjct: 569 GMATKNEDAVTRVFSASTHAPLLFFTSGGKVYKMKVWRLPLGVANSRGKAFVNLLPIEPG 628 Query: 121 ERITTIMPLPEDESSWANLDVMFATTGGTVRRNKLSDFVDVRRSGIIAMKLGEGEAIVDV 180 E IT+I+ LPEDE++W LDVMFAT G+VRRNKLSDFVDVRR+G IAMKL EG+ IV V Sbjct: 629 ETITSILALPEDEATWGGLDVMFATRSGSVRRNKLSDFVDVRRNGKIAMKLDEGDGIVGV 688 Query: 181 QICTERDDVLLTAAGGQCIRFPVTDVRVFTGRTSMGVRGIALAEADKLISLAILRHVETT 240 +C DVLLT A G+CIRF V +VRVF R S GVRG+ L + D++IS+A+LR V+ T Sbjct: 689 AVCNADQDVLLTTAAGRCIRFSVDEVRVFASRDSTGVRGVKLLDGDQVISMAVLRSVDAT 748 Query: 241 SDERSAYLKMRRAVAGEAAAEEPVEA-----EGEETANAIQLSQERYAELSAAEQVVLTV 295 ER+AYLK +RA+ A EE + +G+E L+ ER AEL AAE+++LTV Sbjct: 749 PAERAAYLKHQRAMLRAADGEEGEDTATAADDGDEEVGEASLTPERIAELGAAEEILLTV 808 Query: 296 SVNGYGKRTSSYEYRTTGRGGKGIVAMSVNNRNGNLVASFPVEDADQIMLVTDKGQLIRC 355 S G+GKRTS+Y++R TGRGG+G+ A ++ R G LV SFP++++DQI+LVTD+GQLIR Sbjct: 809 SSEGFGKRTSAYDFRRTGRGGQGLAAQDLSKRGGRLVGSFPIDESDQILLVTDQGQLIRV 868 Query: 356 PVADIRVAGRSTQGVIVFDTAED 378 PV+ IRVA R+TQGV +F TA+D Sbjct: 869 PVSQIRVAARNTQGVTIFRTAQD 891