BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0059.0092.p (215 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr0810 [M] COG3637 Opacity protein and related surface antigens... 103 2e-22 BMEII0844 [M] COG3637 Opacity protein and related surface antige... 100 3e-21 mll1390 [M] COG3637 Opacity protein and related surface antigens... 94 3e-19 mll0155 [M] COG3637 Opacity protein and related surface antigens... 90 4e-18 mll2060 [M] COG3637 Opacity protein and related surface antigens... 88 1e-17 >mlr0810 [M] COG3637 Opacity protein and related surface antigens Mlo Mesorhizobium loti Length = 226 Score = 103 bits (258), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 81/212 (38%), Positives = 107/212 (50%), Gaps = 27/212 (12%) Query: 21 MAARAPYVKAPPPVAIYNWTGFYVGGFGGY------ASEN-SGTPSMEG-------GF-G 65 A A + A P A ++W+G YVGG GY A+ N TP++ G GF G Sbjct: 17 QALAADAISAEPATA-HDWSGVYVGGQIGYGFGKTDATYNLPNTPTIRGSQDYDTDGFLG 75 Query: 66 GGTIGYNWQAGNIVYGLEVDAAGADI----DASVSALGVTARSKIDSLGTVRGRLGFAVD 121 G IGYN+Q + V G+E D +GADI D S LG +K+D GT+R R G+A D Sbjct: 76 GVQIGYNYQINSAVLGVEADVSGADIKGHSDEITSGLGDRYDTKVDWFGTLRARAGYAFD 135 Query: 122 KVLFYGTGGYAW--VNNEVSVTALGVTASQSNFHSGWTVGAGVEAFIAPQWSIKGEYLYR 179 + L YGTGG A+ V N V T S+ N GWT+GAG+E I WS K EY Y Sbjct: 136 RTLIYGTGGLAFGSVENRY-VDGPFDTFSEKNTKVGWTIGAGLEQAITDHWSAKFEYQYV 194 Query: 180 SLDSENYFGVPSGTL----ELHSAQFGVNYHF 207 L + P+ ++ + G+NY F Sbjct: 195 DLRDQTIDYAPNSNTTFDNTFNAVKIGMNYKF 226 >BMEII0844 [M] COG3637 Opacity protein and related surface antigens Bme Brucella melitensis Length = 219 Score = 100 bits (248), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 79/220 (35%), Positives = 106/220 (48%), Gaps = 34/220 (15%) Query: 21 MAARAPYVKAPPPVAIYNWTGFYVGGFGGYA----------------SENSGTPSME-GG 63 M P PV ++WTG Y+G GYA + SG+ + GG Sbjct: 1 MVVSEPSAPTAAPVDTFSWTGGYIGINAGYAGGKFKHPFSSFDKEDNEQVSGSLDVTAGG 60 Query: 64 FGGGT-IGYNWQAGN-IVYGLEVDAAGADIDASVSA----LGVTARSKIDSLGTVRGRLG 117 F GG GYNWQ N +V G E D G+ + S+SA L A +K++ GTVR RLG Sbjct: 61 FVGGVQAGYNWQLDNGVVLGAETDFQGSSVTGSISAGASGLEGKAETKVEWFGTVRARLG 120 Query: 118 F-AVDKVLFYGTGGYAWVNNEVSVTALGVTAS-----QSNFHSGWTVGAGVEAFIAPQWS 171 + A ++++ YGTGG A+ S LG AS +GWT+GAG E I W+ Sbjct: 121 YTATERLMVYGTGGLAY-GKVKSAFNLGDDASALHTWSDKTKAGWTLGAGAEYAINNNWT 179 Query: 172 IKGEYLYRSLDSENYFGVPSGTLE----LHSAQFGVNYHF 207 +K EYLY L N V + LE H+ + G+NY F Sbjct: 180 LKSEYLYTDLGKRNLVDVDNSFLESKVNFHTVRVGLNYKF 219 >mll1390 [M] COG3637 Opacity protein and related surface antigens Mlo Mesorhizobium loti Length = 225 Score = 93.6 bits (231), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 76/230 (33%), Positives = 108/230 (46%), Gaps = 34/230 (14%) Query: 5 AGMFALSALAPAMAADMAARAPYVKAPPPVAIYNWTGFYVGGF--GGYASENS------G 56 A +F S A+ A A A P + YNWTG Y+G GG++ + Sbjct: 3 ASIFIGSVFFAALMAGTALAADATVELPVASSYNWTGGYIGAQIGGGWSKVDQPWGFTEA 62 Query: 57 TPSM--------EGGFGGGTIGYNWQAGNIVYGLEVDAAGADIDASVSALGVTARS---- 104 +P++ G GG GYNWQ+G+ V+G E ADI+A+ Sbjct: 63 SPNIFDQDNADGSGVVGGLHAGYNWQSGSFVFGGE-----ADINATGIDGDDGGSGGDIN 117 Query: 105 --KIDSLGTVRGRLGFAVDKVLFYGTGGYAWVNNEVSVTALGVTASQSNFHSGWTVGAGV 162 K + +VR R GFA D+VL YGTGGYA++N + +G S S +GWT+GAG Sbjct: 118 GFKARWVASVRARAGFAFDRVLIYGTGGYAYLNGKADTRDVGRQESHSASFNGWTIGAGA 177 Query: 163 EAFIAPQWSIKGEYLYRSLDSE-----NYFGVPSGTLELHSAQFGVNYHF 207 E + +++GEY Y S+ NY V + LH+ GV+Y F Sbjct: 178 EYALTDNITVRGEYRYADFGSKTVVFNNY--VEDISPALHTVTIGVSYKF 225 >mll0155 [M] COG3637 Opacity protein and related surface antigens Mlo Mesorhizobium loti Length = 221 Score = 90.1 bits (222), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 70/200 (35%), Positives = 106/200 (53%), Gaps = 19/200 (9%) Query: 1 LLASAGMFALSALAPAMAADMAARAPYVKAPPPVAIYNWTGFYVG---GFGGYASENSGT 57 ++ASAG+ A+ ++ PA+AAD P V P ++WTG+Y G G+G S+ SGT Sbjct: 5 VVASAGLLAVLSV-PALAAD-----PTVDVPMTAPGFDWTGYYAGLQAGYGWGQSDISGT 58 Query: 58 --------PSMEGGFGGGTIGYNWQAGNIVYGLEVDAAGADIDASVSAL-GVTARSKIDS 108 P + GGF GG I WQ V G E D + ++ + A G + I Sbjct: 59 EGEPFSVSPDINGGFVGGHIAGLWQFDQAVIGAEADLNYSSVNGTAEAEPGNNFGTDIKW 118 Query: 109 LGTVRGRLGFAVDKVLFYGTGGYAWVNNEVSVTALGVTASQSNFHSGWTVGAGVEAFIAP 168 G+V + G+A+D+VL YG GG A+ E S +A G S++ + GWT+GAGV+ + Sbjct: 119 FGSVNAKAGYAMDRVLVYGIGGVAFAGIETSQSA-GSAFSETRANVGWTLGAGVDYALTD 177 Query: 169 QWSIKGEYLYRSLDSENYFG 188 ++ + +Y Y SE+Y G Sbjct: 178 KFVVGAQYRYYDFGSEHYDG 197 >mll2060 [M] COG3637 Opacity protein and related surface antigens Mlo Mesorhizobium loti Length = 220 Score = 88.2 bits (217), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 78/234 (33%), Positives = 107/234 (45%), Gaps = 43/234 (18%) Query: 1 LLASAGMFALSALAPAMAADMAARAPYVKAPPPVAIYNWTGFY--VGGFGGY-------A 51 LL S L+A +P +AAD+ P ++WTGFY V GG+ A Sbjct: 5 LLCSVAF--LAAASPVLAADIVE-------PVAAMPFSWTGFYGGVQAGGGWNDSRWSGA 55 Query: 52 SENSGTPSMEGGFGGGTIGYNWQAGNIVYGLEVDAAGADI--DASVS-ALGVTARSKIDS 108 + +S + GG GG IGYN+Q V G+E D AG+ + D S ALG T +K D Sbjct: 56 TFDSFNTNGSGGIFGGQIGYNYQINQFVIGIEGDLAGSTVKGDGQCSTALGTTCETKQDY 115 Query: 109 LGTVRGRLGFAVDKVLFYGTGGYAWVNNEVSVTALGVTASQSNFHS--------GWTVGA 160 LG+VRGRLG+A+D++L YG G A+ ++ A FH GWT G Sbjct: 116 LGSVRGRLGYAIDRILIYGDAGVAFTKYKI--------AEVDGFHQSFGGGSRVGWTAGL 167 Query: 161 GVEAFIAPQWSIKGEYLYRSLDSENYFGVPSGTLELHSAQFGVNYHFGGPIVAK 214 G E + W+ E+ Y FG SG + F N + +V K Sbjct: 168 GAEYALTDHWTAGVEWNYYD------FGTKSGESDTIPGFFVDNRNTENVVVGK 215