BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0059.0036.p (471 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1448 [G] COG0469 Pyruvate kinase Rso Ralstonia solanacearum 490 e-138 PA1498 [G] COG0469 Pyruvate kinase Pae Pseudomonas aeruginosa 477 e-134 BMEI0292 [G] COG0469 Pyruvate kinase Bme Brucella melitensis 464 e-130 AGl2146 [G] COG0469 Pyruvate kinase Atu Agrobacterium tumefacien... 458 e-128 mll3819 [G] COG0469 Pyruvate kinase Mlo Mesorhizobium loti 457 e-128 >RSp1448 [G] COG0469 Pyruvate kinase Rso Ralstonia solanacearum Length = 472 Score = 490 bits (1262), Expect = e-138, Method: Compositional matrix adjust. Identities = 256/471 (54%), Positives = 326/471 (69%), Gaps = 1/471 (0%) Query: 1 MRRHRRAKIVATVGPASSSPEMLEALLLAGVDTFRLNFSHGTQSDHARIHAAIRKLEQEV 60 M+R AKIVAT+GPAS +EAL AG D FRLNFSHG +DH R IR +E + Sbjct: 1 MQRSSHAKIVATLGPASDDHATIEALARAGADVFRLNFSHGAHADHQRRLERIRAVEAAL 60 Query: 61 GRPIGILMDLQGPKIRVGTLRDGKIAAAAGETIRFVL-SGSDGDRTAIPLPHGEIFAAVA 119 GRP+G+L+DLQGPK+RVGT +G++ G R L +G GD T + LPH EIFAA+ Sbjct: 61 GRPLGVLLDLQGPKLRVGTFAEGRVRLDDGAPFRLDLETGRPGDATRVALPHPEIFAAIR 120 Query: 120 PGHDLLIDDGRVRVRVTGLGDDFIEAKVIVGGTISNHKGVNLPGTVLDLSPLTAKDRLDL 179 PG LL+DDG++R+ V G D E +V+ GG +S+ KGVN+PG VL LS LT KDR DL Sbjct: 121 PGVALLLDDGKLRLEVVACGPDHAETRVVNGGMLSDRKGVNVPGVVLPLSALTEKDRADL 180 Query: 180 EFGLKLGVDWVALSFVQKPSDIIEARGLIGDRAGLMAKIEKPAALERIDDIIQLCDAIMV 239 FGL LGVDW+ALSFVQ+ +DI E R L+ RAG++AK+EKPAA++ +D I+ DA+MV Sbjct: 181 AFGLALGVDWIALSFVQRAADIAEIRQLVHGRAGIVAKLEKPAAIDSLDAILAETDAVMV 240 Query: 240 ARGDLGVEIPHEDVPGRQKELVRACRLAVKPVIVATQMLDSMVAAPTPTRAEVSDVATAI 299 ARGDLGVE+P E VP QK +VRACR KPVIVATQML+SM+ AP PTRAE SDVATA+ Sbjct: 241 ARGDLGVELPAEQVPAIQKRIVRACRRLGKPVIVATQMLESMIGAPVPTRAEASDVATAV 300 Query: 300 YDGADAVMLSAESATGRYPREAVEMMDRIIRSTEQHKMYRSIVEATQPGEEQTPPHAVAT 359 YDGADAVMLSAE+A+G++P EAV MM RII TE YR+ ++A+ P A+ Sbjct: 301 YDGADAVMLSAETASGQFPVEAVAMMRRIIAQTEADPHYRAAIDASHTPPGANTPDAIGC 360 Query: 360 AAADLASVVHAAAIVAYTSSGTTAARVARKRPSRPILAITPSREVSRRLCLLWGAHSVLS 419 AA +A ++ AA+VAYTSSG +A R+AR+RP IL +TP E +RRL L+WG H V+ Sbjct: 361 AAHSVAGLLDVAAMVAYTSSGASALRMARERPRAAILGMTPRPETARRLALVWGVHPVIC 420 Query: 420 DDVQSYEEMVERATTFAHAEQFASSRDLLVVVAGIPFGQAGTTNNLRVVSL 470 D V E M + A A +FA + LV+ AG+PFG G+TN LR+ + Sbjct: 421 DGVLDVEAMTDVARQTAVQRRFAQAGQTLVIAAGLPFGTPGSTNLLRIAQV 471 >PA1498 [G] COG0469 Pyruvate kinase Pae Pseudomonas aeruginosa Length = 477 Score = 477 bits (1227), Expect = e-134, Method: Compositional matrix adjust. Identities = 244/467 (52%), Positives = 325/467 (69%) Query: 1 MRRHRRAKIVATVGPASSSPEMLEALLLAGVDTFRLNFSHGTQSDHARIHAAIRKLEQEV 60 M ++AKI+AT+GPA+ S + + AL+ AG + RLNFSHG +DHA+ A +R++E E+ Sbjct: 1 MTADKKAKILATLGPATRSRDDIRALVEAGANLLRLNFSHGDYADHAQRFAWVREVEAEL 60 Query: 61 GRPIGILMDLQGPKIRVGTLRDGKIAAAAGETIRFVLSGSDGDRTAIPLPHGEIFAAVAP 120 PIG+LMDLQGPK+RVG G + G+T LS + GD + LPH EI A+ P Sbjct: 61 NYPIGVLMDLQGPKLRVGRFAAGAVQLQRGQTFTLDLSDAPGDERRVNLPHPEIIHALEP 120 Query: 121 GHDLLIDDGRVRVRVTGLGDDFIEAKVIVGGTISNHKGVNLPGTVLDLSPLTAKDRLDLE 180 G LL+DDG++R+ V D IE +V VGG +S+ KGVN+P VL LSPLT KDR DL Sbjct: 121 GMSLLLDDGKIRLEVVNCHSDAIETRVAVGGELSDRKGVNVPEAVLQLSPLTDKDRRDLA 180 Query: 181 FGLKLGVDWVALSFVQKPSDIIEARGLIGDRAGLMAKIEKPAALERIDDIIQLCDAIMVA 240 FGL+LGVDWVALSFVQ+P DI EARGLIGD+A LMAKIEKP+A+ I+ I + DAIMVA Sbjct: 181 FGLELGVDWVALSFVQRPEDIDEARGLIGDKAFLMAKIEKPSAVSAIEAIAERADAIMVA 240 Query: 241 RGDLGVEIPHEDVPGRQKELVRACRLAVKPVIVATQMLDSMVAAPTPTRAEVSDVATAIY 300 RGDLGVE+P E VPG QK +V+ CR KPV+VATQML+SM +P PTRAEV+DVATA+ Sbjct: 241 RGDLGVEVPAESVPGIQKRIVQVCRQLGKPVVVATQMLESMRFSPAPTRAEVTDVATAVG 300 Query: 301 DGADAVMLSAESATGRYPREAVEMMDRIIRSTEQHKMYRSIVEATQPGEEQTPPHAVATA 360 GADAVMLSAE+A+G+YPREAVEMM +I+R E Y +E +P + T A++ A Sbjct: 301 AGADAVMLSAETASGQYPREAVEMMAKIVRQVEAEPDYHVQLEVNRPQPDATVSDAISCA 360 Query: 361 AADLASVVHAAAIVAYTSSGTTAARVARKRPSRPILAITPSREVSRRLCLLWGAHSVLSD 420 ++ ++ A +V YT SG + R AR+RP PIL++TP+ +RRL + WG +SV+++ Sbjct: 361 IRRVSRILPVAVLVNYTESGNSTLRAARERPKAPILSLTPNLRTARRLTVAWGVYSVVNE 420 Query: 421 DVQSYEEMVERATTFAHAEQFASSRDLLVVVAGIPFGQAGTTNNLRV 467 + +E+ A A A++ A D +VV AG+PFG+ G+TN LR+ Sbjct: 421 QLAHVDEICSTALDIALAQRMARRGDTVVVTAGVPFGRPGSTNMLRI 467 >BMEI0292 [G] COG0469 Pyruvate kinase Bme Brucella melitensis Length = 478 Score = 464 bits (1194), Expect = e-130, Method: Compositional matrix adjust. Identities = 233/467 (49%), Positives = 315/467 (67%) Query: 1 MRRHRRAKIVATVGPASSSPEMLEALLLAGVDTFRLNFSHGTQSDHARIHAAIRKLEQEV 60 M+R+R+ KI+AT+GPAS ++ L AG D FR+N SH + IR +E+E+ Sbjct: 1 MKRNRKVKILATLGPASGEEAVIRKLFEAGADVFRINMSHADHDLMRTLVKRIRNVEKEL 60 Query: 61 GRPIGILMDLQGPKIRVGTLRDGKIAAAAGETIRFVLSGSDGDRTAIPLPHGEIFAAVAP 120 GRPIGIL DLQGPK+RVG +DGK+ G+T + + GD T + LPH EI AV P Sbjct: 61 GRPIGILADLQGPKLRVGKFKDGKVDLVPGQTFTLDNNEALGDETRVFLPHPEILEAVEP 120 Query: 121 GHDLLIDDGRVRVRVTGLGDDFIEAKVIVGGTISNHKGVNLPGTVLDLSPLTAKDRLDLE 180 GH LLIDDG++ + I KV+ G IS+ KGV+LP T L + LT KDR DL+ Sbjct: 121 GHRLLIDDGKLHLVAEACDGKSIRCKVVSGTRISDRKGVSLPDTTLGVGALTEKDRCDLD 180 Query: 181 FGLKLGVDWVALSFVQKPSDIIEARGLIGDRAGLMAKIEKPAALERIDDIIQLCDAIMVA 240 LK +DWVALSF+Q+P D+ E R + + GLM+KIEKP A+ R+D+II+L DA+MVA Sbjct: 181 AVLKEEIDWVALSFIQRPEDLAEVRKVSRGKVGLMSKIEKPQAVTRLDEIIELSDALMVA 240 Query: 241 RGDLGVEIPHEDVPGRQKELVRACRLAVKPVIVATQMLDSMVAAPTPTRAEVSDVATAIY 300 RGDLGVE+P E+VPG QK++ R R A KPV+VATQML+SM+ AP PTRAEVSDVATA++ Sbjct: 241 RGDLGVEVPLENVPGIQKQITRKARRAGKPVVVATQMLESMITAPVPTRAEVSDVATAVF 300 Query: 301 DGADAVMLSAESATGRYPREAVEMMDRIIRSTEQHKMYRSIVEATQPGEEQTPPHAVATA 360 +GADA+MLSAESA+G+YP EAV M+RI E+ Y +I++A + E T A++ A Sbjct: 301 EGADAIMLSAESASGQYPVEAVATMNRIAEQVEREPTYSTIIDAQRAMPEPTGADAISLA 360 Query: 361 AADLASVVHAAAIVAYTSSGTTAARVARKRPSRPILAITPSREVSRRLCLLWGAHSVLSD 420 A +A + +AIV YT+SGTT R AR+RP PI+A++P + +R+L L+WG H V++D Sbjct: 361 ARQIAETLKLSAIVTYTASGTTGLRAARERPRTPIIALSPVVDTARKLSLVWGLHCVITD 420 Query: 421 DVQSYEEMVERATTFAHAEQFASSRDLLVVVAGIPFGQAGTTNNLRV 467 D E+MV A E+F + D +++ AG+PFG G TN LR+ Sbjct: 421 DAHDLEDMVNHACEIVFHEEFGKAGDRVIITAGVPFGTPGATNMLRI 467 >AGl2146 [G] COG0469 Pyruvate kinase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 479 Score = 458 bits (1179), Expect = e-128, Method: Compositional matrix adjust. Identities = 242/470 (51%), Positives = 316/470 (67%), Gaps = 5/470 (1%) Query: 1 MRRHRRAKIVATVGPASSSPEMLEALLLAGVDTFRLNFSHGTQSDHARIHAAIRKLEQEV 60 M+R+R+ KI+AT+GPASS M+E L LAG D FR+N SH + + IR +E Sbjct: 1 MKRNRKVKILATLGPASSEEAMIEKLHLAGADLFRINMSHASHDVMRTLIQRIRSVESRN 60 Query: 61 GRPIGILMDLQGPKIRVGTLRDGKIAAAAGETIRFVLSGSD--GDRTAIPLPHGEIFAAV 118 GRPIGIL DLQGPK+RVG + K+ G+T F L +D GD T + LPH EI AV Sbjct: 61 GRPIGILADLQGPKLRVGKFAETKVDLVPGQT--FTLDNNDTPGDNTRVFLPHPEILEAV 118 Query: 119 APGHDLLIDDGRVRVRVTGLGDDFIEAKVIVGGTISNHKGVNLPGTVLDLSPLTAKDRLD 178 PGH LLIDDG++ +R I V+ G IS+ KG++LP T+L + LT KDR+D Sbjct: 119 KPGHRLLIDDGKLHLRAEKSDGKSIVTTVVSGTRISDRKGISLPDTLLGVGVLTDKDRVD 178 Query: 179 LEFGLKLG-VDWVALSFVQKPSDIIEARGLIGDRAGLMAKIEKPAALERIDDIIQLCDAI 237 L+ L+ VDWVALSFVQ+P D+ E R + R GLM+KIEKP A+ERI++II+L DA+ Sbjct: 179 LDAVLETNEVDWVALSFVQRPEDLAEVRKISRGRVGLMSKIEKPQAVERIEEIIELSDAL 238 Query: 238 MVARGDLGVEIPHEDVPGRQKELVRACRLAVKPVIVATQMLDSMVAAPTPTRAEVSDVAT 297 MVARGDLGVE+P E VPG QK+L RACR A KPV+VATQML+SM+ AP PTRAEVSDVAT Sbjct: 239 MVARGDLGVEMPLEAVPGIQKQLTRACRRAGKPVVVATQMLESMITAPVPTRAEVSDVAT 298 Query: 298 AIYDGADAVMLSAESATGRYPREAVEMMDRIIRSTEQHKMYRSIVEATQPGEEQTPPHAV 357 A+++GADA+MLSAESA+G YP EAV M I + EQ Y +I+ A +P E T A+ Sbjct: 299 AVFEGADAIMLSAESASGDYPIEAVSTMASIASTVEQDPYYSNIIYAQRPQPEATGADAI 358 Query: 358 ATAAADLASVVHAAAIVAYTSSGTTAARVARKRPSRPILAITPSREVSRRLCLLWGAHSV 417 + AA +A + AAIV YTSSGTT R AR+RP PI+A++P + +RRL ++WG H V Sbjct: 359 SLAARQIAETLKLAAIVTYTSSGTTGLRAARERPQVPIIALSPIIQTARRLSVVWGLHCV 418 Query: 418 LSDDVQSYEEMVERATTFAHAEQFASSRDLLVVVAGIPFGQAGTTNNLRV 467 ++ D ++MV RA +E F D +++ AG+P G G TN +R+ Sbjct: 419 VTGDASDLDDMVNRACRIVVSEGFGKPGDRIIISAGVPLGTPGATNMVRI 468 >mll3819 [G] COG0469 Pyruvate kinase Mlo Mesorhizobium loti Length = 478 Score = 457 bits (1175), Expect = e-128, Method: Compositional matrix adjust. Identities = 238/467 (50%), Positives = 310/467 (66%) Query: 1 MRRHRRAKIVATVGPASSSPEMLEALLLAGVDTFRLNFSHGTQSDHARIHAAIRKLEQEV 60 MRR R+ KI+AT+GPASSS EML+ L AG D FR+N SH + IR +E++V Sbjct: 1 MRRSRKVKILATIGPASSSEEMLQKLFEAGADVFRINMSHTDHELMRTLVGRIRAVEEKV 60 Query: 61 GRPIGILMDLQGPKIRVGTLRDGKIAAAAGETIRFVLSGSDGDRTAIPLPHGEIFAAVAP 120 GRPIGIL DLQGPK+RVG +GK G+T + G + + LPH EI ++V P Sbjct: 61 GRPIGILADLQGPKLRVGKFANGKEVLTPGQTFTLDDNPEPGTSSRVYLPHPEILSSVEP 120 Query: 121 GHDLLIDDGRVRVRVTGLGDDFIEAKVIVGGTISNHKGVNLPGTVLDLSPLTAKDRLDLE 180 GH LLIDDG++ ++ I V+ G TIS+ KGV+LP T L + LT KDR DL+ Sbjct: 121 GHRLLIDDGKLELKAVKSDGKSIVCTVVAGTTISDKKGVSLPDTDLPVGALTEKDRKDLD 180 Query: 181 FGLKLGVDWVALSFVQKPSDIIEARGLIGDRAGLMAKIEKPAALERIDDIIQLCDAIMVA 240 L GVDW+ALSFVQ+P D+ EAR + RA +MAKIEKP A+ R+ +II+L DA+MVA Sbjct: 181 AVLATGVDWIALSFVQRPEDLAEARKIARGRALIMAKIEKPQAVARLAEIIELSDALMVA 240 Query: 241 RGDLGVEIPHEDVPGRQKELVRACRLAVKPVIVATQMLDSMVAAPTPTRAEVSDVATAIY 300 RGDLGVE+P E VPG QK++ RA R A KPV+VATQML+SM+ AP PTRAEVSDV+ A++ Sbjct: 241 RGDLGVEMPLEAVPGIQKQITRAARRAGKPVVVATQMLESMITAPVPTRAEVSDVSIAVF 300 Query: 301 DGADAVMLSAESATGRYPREAVEMMDRIIRSTEQHKMYRSIVEATQPGEEQTPPHAVATA 360 +GADA+MLSAESA G YP EAV MMDRI E Y I+ A + E T A++ A Sbjct: 301 EGADAIMLSAESAAGAYPVEAVAMMDRIATKVETDPTYAGIINAQRSEPEATGADAISLA 360 Query: 361 AADLASVVHAAAIVAYTSSGTTAARVARKRPSRPILAITPSREVSRRLCLLWGAHSVLSD 420 A ++A + +AI+ YT+SGTT R AR+RP PI+A++P +RRL LLWG H V+S Sbjct: 361 AREIAETLKLSAIITYTASGTTGLRAARERPQVPIIALSPILNTARRLSLLWGTHCVVSP 420 Query: 421 DVQSYEEMVERATTFAHAEQFASSRDLLVVVAGIPFGQAGTTNNLRV 467 D ++MV RA A E+F D +++ AG+P G+TN LR+ Sbjct: 421 DATDLDDMVNRACRIALEEEFGKPGDRVIITAGVPLRTPGSTNMLRI 467