BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0050.0062.p (491 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Rv3375 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 353 4e-97 MT3485 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 353 4e-97 PA4483 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 267 4e-71 NMB1356 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 263 9e-70 NMA1568 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 261 4e-69 >Rv3375 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Mtu Mycobacterium tuberculosis H37Rv Length = 475 Score = 353 bits (907), Expect = 4e-97, Method: Compositional matrix adjust. Identities = 200/463 (43%), Positives = 282/463 (60%), Gaps = 3/463 (0%) Query: 24 RSTENQMHYLTLSELSVLLRTKAISPVELVKHMLGRIDALEPRLESYVTITAERALEQAE 83 R E+ + L L+E++ L+RT+ ++ E+ + L RI+ L+P+L+SY + E AL A Sbjct: 11 RLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAAR 70 Query: 84 RAEREIGSGQWRGHLHGVPLAYKDIFYTDFALTTAGTAIHKSFQPTYSATAVRRLEAAGA 143 A+ +I G + G LHGVP+ KD+ YT A T AGT I + F+P Y AT V RL AAGA Sbjct: 71 AADADIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGA 130 Query: 144 VSLGKLKTTEQAWTEHHPAVAAPKNPWGAELYAGASSSGSGVATAAGLTFGALGSDTGGS 203 V +GKL TE A+ +HP++ P NPW +AG SSSG GVATAAGL FG++GSDTGGS Sbjct: 131 VIIGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGS 190 Query: 204 IRFPSAVNGVTGLKPTWGRVSRHGVFELAASLDHVGPMARSAIDCAIMLGAIAGADPADP 263 IRFP+++ GVTG+KPTWGRVSRHGV ELAAS DHVGP+ RSA D A++L IAG+D DP Sbjct: 191 IRFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSVIAGSDIHDP 250 Query: 264 TAVLNPIEDYEAAALRSVAGMRIGLPRGYATEGVDPGIVAVWQAAAKVFEDLGAIVEEIS 323 + P+ DY AA L R+G+ T D A+ K +D+G V ++ Sbjct: 251 SCSAEPVPDY-AADLALTRIPRVGVDWSQTTS-FDEDTTAMLADVVKTLDDIGWPVIDVK 308 Query: 324 FPEWRKAASTWPILCSSETALTHAATYPSKSESYGTALSALIDLGRSSTAMDLAAAHQAR 383 P + + + + ETA+ HA TYP++++ YG + A+ID G A++ + R Sbjct: 309 LPALAPMVAAFGKMRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVEYQTLTERR 368 Query: 384 LEFSSRLRARFDNLDVILVPATIWPTPTAAATPDILQKSPLEDLLRF-TCVFDSSGSPTL 442 LEF+ LR F ++D++L+P+ +PT + Q L L T F+ SG+P + Sbjct: 369 LEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPFNVSGNPAI 428 Query: 443 TLPGGFDGGGTPMAIQLIGAHFDEAAVLQAGAAFQRATDWHSK 485 LP G GTP+ +Q IG FDE +++AG AFQ+ T +H + Sbjct: 429 CLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRR 471 >MT3485 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases MtC Mycobacterium tuberculosis CDC1551 Length = 475 Score = 353 bits (907), Expect = 4e-97, Method: Compositional matrix adjust. Identities = 200/463 (43%), Positives = 282/463 (60%), Gaps = 3/463 (0%) Query: 24 RSTENQMHYLTLSELSVLLRTKAISPVELVKHMLGRIDALEPRLESYVTITAERALEQAE 83 R E+ + L L+E++ L+RT+ ++ E+ + L RI+ L+P+L+SY + E AL A Sbjct: 11 RLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAAR 70 Query: 84 RAEREIGSGQWRGHLHGVPLAYKDIFYTDFALTTAGTAIHKSFQPTYSATAVRRLEAAGA 143 A+ +I G + G LHGVP+ KD+ YT A T AGT I + F+P Y AT V RL AAGA Sbjct: 71 AADADIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGA 130 Query: 144 VSLGKLKTTEQAWTEHHPAVAAPKNPWGAELYAGASSSGSGVATAAGLTFGALGSDTGGS 203 V +GKL TE A+ +HP++ P NPW +AG SSSG GVATAAGL FG++GSDTGGS Sbjct: 131 VIIGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGS 190 Query: 204 IRFPSAVNGVTGLKPTWGRVSRHGVFELAASLDHVGPMARSAIDCAIMLGAIAGADPADP 263 IRFP+++ GVTG+KPTWGRVSRHGV ELAAS DHVGP+ RSA D A++L IAG+D DP Sbjct: 191 IRFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSVIAGSDIHDP 250 Query: 264 TAVLNPIEDYEAAALRSVAGMRIGLPRGYATEGVDPGIVAVWQAAAKVFEDLGAIVEEIS 323 + P+ DY AA L R+G+ T D A+ K +D+G V ++ Sbjct: 251 SCSAEPVPDY-AADLALTRIPRVGVDWSQTTS-FDEDTTAMLADVVKTLDDIGWPVIDVK 308 Query: 324 FPEWRKAASTWPILCSSETALTHAATYPSKSESYGTALSALIDLGRSSTAMDLAAAHQAR 383 P + + + + ETA+ HA TYP++++ YG + A+ID G A++ + R Sbjct: 309 LPALAPMVAAFGKMRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVEYQTLTERR 368 Query: 384 LEFSSRLRARFDNLDVILVPATIWPTPTAAATPDILQKSPLEDLLRF-TCVFDSSGSPTL 442 LEF+ LR F ++D++L+P+ +PT + Q L L T F+ SG+P + Sbjct: 369 LEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPFNVSGNPAI 428 Query: 443 TLPGGFDGGGTPMAIQLIGAHFDEAAVLQAGAAFQRATDWHSK 485 LP G GTP+ +Q IG FDE +++AG AFQ+ T +H + Sbjct: 429 CLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRR 471 >PA4483 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Pae Pseudomonas aeruginosa Length = 484 Score = 267 bits (683), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 179/490 (36%), Positives = 267/490 (54%), Gaps = 36/490 (7%) Query: 30 MHYLTLSELSVLLRTKAISPVELVKHMLGRIDALEPRLESYVTITAERALEQAERAEREI 89 +H LTL+E++ L K S EL + +LGRI L+P+L S+++IT + A+ QA+ A+ Sbjct: 2 LHQLTLAEIARALADKQFSAEELTRTLLGRIRQLDPQLNSFISITDDLAIAQAKAADERR 61 Query: 90 GSGQWRGHLHGVPLAYKDIFYTDFALTTAGTAIHKSFQPTYSATAVRRLEAAGAVSLGKL 149 +G+ G L G P+A+KD+F T T+ G+ + +F Y AT V +L AAGAV+LGKL Sbjct: 62 ANGE-NGALLGAPIAHKDLFCTQGVRTSCGSKMLDNFVSPYDATVVEKLTAAGAVTLGKL 120 Query: 150 KTTEQAW-----TEHHPAVAAPKNPWGAELYAGASSSGSGVATAAGLTFGALGSDTGGSI 204 E A + H+ AV KNPW + G SS GS A AA L A G+DTGGSI Sbjct: 121 NMDEFAMGSSNQSSHYGAV---KNPWSLDRVPGGSSGGSAAAVAARLLPAATGTDTGGSI 177 Query: 205 RFPSAVNGVTGLKPTWGRVSRHGVFELAASLDHVGPMARSAIDCAIMLGAIAGADPADPT 264 R P+A+ +TG+KPT+GRVSR G+ A+SLD GP+AR+A DCA+MLG +AG DP D T Sbjct: 178 RQPAALTNLTGIKPTYGRVSRWGMIAYASSLDQGGPLARTAEDCALMLGVMAGFDPKDST 237 Query: 265 AVLNPIEDYEAAALRSVAGMRIGLPRGYATEGVDPGIVAVWQAAAKVFEDLGAIVEEISF 324 +V P++DY AA + ++G+RIGLPR Y G+D I A + + LGA V++IS Sbjct: 238 SVEQPVDDYLAALQKPLSGLRIGLPREYFGAGLDSRIADAVLAVVEELKTLGATVKDISL 297 Query: 325 PEWRKAASTWPILCSSETALT----------HAATYP---------SKSESYGTALSALI 365 P + A + ++ +E + + P S++E +G+ + I Sbjct: 298 PNMQHAIPAYYVIAPAEASSNLSRFDGVRYGYRCDAPQNLEDLYKRSRAEGFGSEVKNRI 357 Query: 366 DLGRSSTAMDLAAAHQARLEFSSRLRAR-----FDNLDVILVPATIWPTPTAAATPDILQ 420 +G + + A+ + + RL F +DVIL P T P D Sbjct: 358 MVGTYALSAGYYDAYYLQAQKIRRLIKNDFVSAFAEVDVILGPTTPNPAWKIGEKNDDPV 417 Query: 421 KSPLEDLLRFTCVFDSSGSPTLTLPGGFDGGGTPMAIQLIGAHFDEAAVLQAGAAFQRAT 480 LED+ +T + +G P L++P GF G P+ +QL+ +F E +L +Q+ + Sbjct: 418 SQYLEDI--YTITANLAGLPGLSMPAGF-VDGLPVGVQLLAPYFQEGRLLNVAHQYQQVS 474 Query: 481 DWHSKHSRHF 490 DWH++ F Sbjct: 475 DWHTRTPAGF 484 >NMB1356 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Nme Neisseria meningitidis MC58 Length = 481 Score = 263 bits (671), Expect = 9e-70, Method: Compositional matrix adjust. Identities = 177/487 (36%), Positives = 249/487 (51%), Gaps = 38/487 (7%) Query: 30 MHYLTLSELSVLLRTKAISPVELVKHMLGRIDALEPRLESYVTITAERALEQAERAEREI 89 M TL + SVLL++K IS VEL L I P L Y+TI ++ L +A A+ I Sbjct: 1 MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITIDQDKTLAEARAADERI 60 Query: 90 GSGQWRGHLHGVPLAYKDIFYTDFALTTAGTAIHKSFQPTYSATAVRRLEAAGAVSLGKL 149 G L GVP+AYKDIF + + + +F Y+AT V+ L G V+LG+ Sbjct: 61 AQGNASA-LTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTATVVQNLLDEGMVTLGRT 119 Query: 150 KTTEQAW--TEHHPAVAAPKNPWGAELYAGASSSGSGVATAAGLTFGALGSDTGGSIRFP 207 E A T + A KNPW E G SS GS AA L ALGSDTGGSIR P Sbjct: 120 NMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVAARLAPAALGSDTGGSIRQP 179 Query: 208 SAVNGVTGLKPTWGRVSRHGVFELAASLDHVGPMARSAIDCAIMLGAIAGADPADPTAVL 267 ++ G+TG+KPT+G VSR G+ A+S D GPMA++A DCAI+L A+AG DP D T++ Sbjct: 180 ASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAEDCAILLNAMAGFDPKDSTSLE 239 Query: 268 NPIEDYEAAALRSVAGMRIGLPRGYATEGVDPGIVAVWQAAAKVFEDLGAIVEEISFPEW 327 EDY + + G++IGLP+ Y EG ++ Q + + GA + E+S P+ Sbjct: 240 REKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTALQNTIDLLKAQGAELIEVSLPQT 299 Query: 328 RKAASTWPILCSSETALTHAATY--------------------PSKSESYGTALSALIDL 367 + + + +L S+E A T+ + Y +++E +G+ + I + Sbjct: 300 KLSIPAYYVLASAE-ASTNLSRYDGVRYGHRAAQFADLEEMYGKTRAEGFGSEVKRRIMI 358 Query: 368 GRSSTAMDLAAAHQARLEFSSRLRAR-----FDNLDVILVPATIWPTPTAAATPDI-LQK 421 G + A+ + + RL A F D+IL P PTAA P I Sbjct: 359 GTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFARCDLILAPT----APTAA--PKIGADA 412 Query: 422 SPLEDLLR--FTCVFDSSGSPTLTLPGGFDGGGTPMAIQLIGAHFDEAAVLQAGAAFQRA 479 SP+E L +T + +G P LTLP GF GGG P+ +QL+G +F EA +L A Q Sbjct: 413 SPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGVQLVGNYFAEAKILGAAHQIQLN 472 Query: 480 TDWHSKH 486 +DWH K Sbjct: 473 SDWHGKR 479 >NMA1568 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases NmA Neisseria meningitidis Z2491 Length = 481 Score = 261 bits (666), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 175/487 (35%), Positives = 249/487 (51%), Gaps = 38/487 (7%) Query: 30 MHYLTLSELSVLLRTKAISPVELVKHMLGRIDALEPRLESYVTITAERALEQAERAEREI 89 M TL + SVLL++K IS VEL L I P L Y+TI ++ L +A A+ I Sbjct: 1 MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITIDQDKTLAEARAADERI 60 Query: 90 GSGQWRGHLHGVPLAYKDIFYTDFALTTAGTAIHKSFQPTYSATAVRRLEAAGAVSLGKL 149 G L G+P+AYKDIF + + + +F Y+AT V++L G V+LG+ Sbjct: 61 AQGNASA-LTGIPVAYKDIFCQTGWRSACASKMLDNFISPYTATVVQKLLDEGMVTLGRT 119 Query: 150 KTTEQAW--TEHHPAVAAPKNPWGAELYAGASSSGSGVATAAGLTFGALGSDTGGSIRFP 207 E A T + A KNPW E G SS GS AA L ALGSDTGGSIR P Sbjct: 120 NMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVAARLAPAALGSDTGGSIRQP 179 Query: 208 SAVNGVTGLKPTWGRVSRHGVFELAASLDHVGPMARSAIDCAIMLGAIAGADPADPTAVL 267 ++ G+TG+KPT+G VSR G+ A+S D GPMA++A DCAI+L A+AG DP D T++ Sbjct: 180 ASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAEDCAILLNAMAGFDPKDSTSLE 239 Query: 268 NPIEDYEAAALRSVAGMRIGLPRGYATEGVDPGIVAVWQAAAKVFEDLGAIVEEISFPEW 327 EDY + + G++IGLP+ Y EG ++ Q + + GA + E+S P+ Sbjct: 240 REKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTALQNTIDLLKAQGAELIEVSLPQT 299 Query: 328 RKAASTWPILCSSETALTHAATY--------------------PSKSESYGTALSALIDL 367 + + + +L S+E A T+ + Y +++E +G+ + I + Sbjct: 300 KLSIPAYYVLASAE-ASTNLSRYDGVRYGHRAAQFSDLEEMYGKTRAEGFGSEVKRRIMI 358 Query: 368 GRSSTAMDLAAAHQARLEFSSRLRAR-----FDNLDVILVPATIWPTPTAAATPDI-LQK 421 G + A+ + + RL A F D+IL P P+AA P I Sbjct: 359 GTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFARCDLILAPT----APSAA--PKIGADA 412 Query: 422 SPLEDLLR--FTCVFDSSGSPTLTLPGGFDGGGTPMAIQLIGAHFDEAAVLQAGAAFQRA 479 SP+E L +T + +G P LTLP GF GG P+ +QLIG +F EA +L A Q Sbjct: 413 SPVETYLSDIYTIAVNLAGLPALTLPAGFSSGGLPIGVQLIGNYFSEAKILGAAHQIQLN 472 Query: 480 TDWHSKH 486 +DWH K Sbjct: 473 SDWHGKR 479