BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0050.0003.p (233 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr8347 [J] COG0293 23S rRNA methylase Mlo Mesorhizobium loti 256 3e-68 BMEII0597 [J] COG0293 23S rRNA methylase Bme Brucella melitensis 252 6e-67 SMc00811 [J] COG0293 23S rRNA methylase Sme Sinorhizobium meliloti 249 2e-66 RC0205 [J] COG0293 23S rRNA methylase Rco Rickettsia conorii 244 1e-64 RP163 [J] COG0293 23S rRNA methylase Rpr Rickettsia prowazekii 239 5e-63 >mlr8347 [J] COG0293 23S rRNA methylase Mlo Mesorhizobium loti Length = 265 Score = 256 bits (654), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 123/218 (56%), Positives = 155/218 (71%) Query: 9 LHVTVKSGGKRKLSSKLWLERQLNDPYVAKAKAMGYRSRAAFKLTEIDDKYRLLKPGMTV 68 L +K K SS+ WL+R +NDPYV ++KA GYRSRAA+KL EIDDK+ LLKPGM V Sbjct: 16 LKTRIKKKSGLKESSRRWLQRHINDPYVQRSKADGYRSRAAYKLIEIDDKHHLLKPGMKV 75 Query: 69 VDLGAAPGGWSQIAAKRTGAADGKGKVVAIDLLEMGEIPGVTFAQLDFLDHDAPEKLIAM 128 +DLGAAPGGW Q+AA RT + VV ID LEM +PG +DFLD DAP+KL Sbjct: 76 IDLGAAPGGWCQVAAARTKSTAENPHVVGIDYLEMDAVPGAPVLLMDFLDPDAPQKLAEA 135 Query: 129 MGGRADIVMSDMAANTTGHRKTDQLRIIGLVETAAHFAGEVLNPGGTFLAKVFQSGADAE 188 +GG D+V+SDMAA TTGH++TD +R + L E AA FA VL PGG FLAK FQ GA+ E Sbjct: 136 LGGNPDVVLSDMAAPTTGHKRTDHIRTMHLCEVAADFALSVLKPGGHFLAKTFQGGAENE 195 Query: 189 LVAQLKRDFATVRHVKPAASRQDSSERYVLATGFRGQQ 226 L++ LK++F +V HVKP ASR +S E Y+LA F+G++ Sbjct: 196 LLSMLKKNFRSVHHVKPPASRDESVELYLLAKDFKGRE 233 >BMEII0597 [J] COG0293 23S rRNA methylase Bme Brucella melitensis Length = 240 Score = 252 bits (643), Expect = 6e-67, Method: Compositional matrix adjust. Identities = 124/217 (57%), Positives = 155/217 (71%), Gaps = 1/217 (0%) Query: 9 LHVTVKS-GGKRKLSSKLWLERQLNDPYVAKAKAMGYRSRAAFKLTEIDDKYRLLKPGMT 67 LHV VK G K SS+ WLER LNDPYV K++ GYRSRAA+KL EI+D+Y LLK G Sbjct: 24 LHVRVKKKAGTIKESSRRWLERHLNDPYVHKSRQDGYRSRAAYKLIEINDRYNLLKKGQK 83 Query: 68 VVDLGAAPGGWSQIAAKRTGAADGKGKVVAIDLLEMGEIPGVTFAQLDFLDHDAPEKLIA 127 ++DLGAAPGGWSQIAA+ G+ D +VV ID L + +PGV ++DFLD +AP+KL+ Sbjct: 84 IIDLGAAPGGWSQIAARIVGSTDENPQVVGIDYLHVDPLPGVILLEMDFLDDEAPQKLMD 143 Query: 128 MMGGRADIVMSDMAANTTGHRKTDQLRIIGLVETAAHFAGEVLNPGGTFLAKVFQSGADA 187 +G + D+V+SDMAA TTGHR+TD LR + L E AA FA VL PGG FL K FQ G + Sbjct: 144 ALGDKPDLVISDMAAPTTGHRRTDHLRTVHLCEVAADFAVSVLKPGGHFLTKTFQGGTEN 203 Query: 188 ELVAQLKRDFATVRHVKPAASRQDSSERYVLATGFRG 224 EL+A LK+ F +V HVKP ASR +S E Y+LA F+G Sbjct: 204 ELLALLKQKFRSVHHVKPPASRAESVELYLLARDFKG 240 >SMc00811 [J] COG0293 23S rRNA methylase Sme Sinorhizobium meliloti Length = 245 Score = 249 bits (637), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 121/210 (57%), Positives = 153/210 (72%) Query: 17 GKRKLSSKLWLERQLNDPYVAKAKAMGYRSRAAFKLTEIDDKYRLLKPGMTVVDLGAAPG 76 GK K SS+ W+ER +NDPYV +A+ GYR+RAAFKL EID+K+++L ++DLGAAPG Sbjct: 22 GKLKASSRRWIERHINDPYVQRAQLEGYRARAAFKLLEIDEKHKILAGAKRIIDLGAAPG 81 Query: 77 GWSQIAAKRTGAADGKGKVVAIDLLEMGEIPGVTFAQLDFLDHDAPEKLIAMMGGRADIV 136 WSQIAAK T + D +V AID LEM IPGV F Q+DFLD +APE L +GG DIV Sbjct: 82 SWSQIAAKVTNSTDADPRVAAIDFLEMDPIPGVRFLQMDFLDPEAPENLKQAIGGAPDIV 141 Query: 137 MSDMAANTTGHRKTDQLRIIGLVETAAHFAGEVLNPGGTFLAKVFQSGADAELVAQLKRD 196 +SDMAA TTGHR+TD +R + L E AAHFA EVL GG FLAK FQ G + +L+ LK++ Sbjct: 142 LSDMAAPTTGHRQTDHIRTMHLCEVAAHFAVEVLAEGGHFLAKTFQGGTERDLLNMLKQN 201 Query: 197 FATVRHVKPAASRQDSSERYVLATGFRGQQ 226 F V HVKPA+SR +S E ++LA GF+G+ Sbjct: 202 FRQVVHVKPASSRAESVEMFLLAKGFKGRH 231 >RC0205 [J] COG0293 23S rRNA methylase Rco Rickettsia conorii Length = 227 Score = 244 bits (622), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 113/211 (53%), Positives = 154/211 (72%), Gaps = 2/211 (0%) Query: 11 VTVKSGGKRKLSSKLWLERQLNDPYVAKAKAMGYRSRAAFKLTEIDDKYRLLKPGMTVVD 70 V VK+ KR +SS WL RQLNDPYVAKA+ G+RSRAA+KL EI +K++L P M +VD Sbjct: 13 VRVKTSKKRTVSSSNWLRRQLNDPYVAKARIDGFRSRAAYKLLEIHEKFKLFTPNMKIVD 72 Query: 71 LGAAPGGWSQIAAKRTGAADGK--GKVVAIDLLEMGEIPGVTFAQLDFLDHDAPEKLIAM 128 LGAAPGGWSQ+A+K A+D K+++ID+LE+ + GV F Q DF + D E +I Sbjct: 73 LGAAPGGWSQVASKLIKASDNNLNNKIISIDVLEIEHVAGVEFVQKDFFEADTEELIIQA 132 Query: 129 MGGRADIVMSDMAANTTGHRKTDQLRIIGLVETAAHFAGEVLNPGGTFLAKVFQSGADAE 188 + GRADIVMSDMA+NT GH+ TD +R + L E A FA +VL P G F+AK+F+ GA+ E Sbjct: 133 LDGRADIVMSDMASNTIGHKATDHIRTLLLCEQAFEFALKVLKPSGHFIAKIFRGGAENE 192 Query: 189 LVAQLKRDFATVRHVKPAASRQDSSERYVLA 219 L+ ++KR+F TV+H KP++SR +S+E Y++A Sbjct: 193 LLHKVKREFKTVKHFKPSSSRSESTEIYLVA 223 >RP163 [J] COG0293 23S rRNA methylase Rpr Rickettsia prowazekii Length = 227 Score = 239 bits (609), Expect = 5e-63, Method: Compositional matrix adjust. Identities = 113/211 (53%), Positives = 150/211 (71%), Gaps = 2/211 (0%) Query: 11 VTVKSGGKRKLSSKLWLERQLNDPYVAKAKAMGYRSRAAFKLTEIDDKYRLLKPGMTVVD 70 V VK+ KR +SS WL RQLNDPYVAKA+ G+RSRAA+KL EI K+RL P M +VD Sbjct: 13 VRVKTSKKRPISSSNWLRRQLNDPYVAKARLEGFRSRAAYKLLEIHYKFRLFTPNMKIVD 72 Query: 71 LGAAPGGWSQIAAKRTGAADGK--GKVVAIDLLEMGEIPGVTFAQLDFLDHDAPEKLIAM 128 LGAAPGGWSQ+A+K A+D K+++IDLLE+ I GV F Q DF + D E +I Sbjct: 73 LGAAPGGWSQVASKLIKASDNNLNNKIISIDLLEIEPIVGVEFLQKDFFEKDTEELIIHN 132 Query: 129 MGGRADIVMSDMAANTTGHRKTDQLRIIGLVETAAHFAGEVLNPGGTFLAKVFQSGADAE 188 + G+ADIVMSDMA+NT GH+ TD +R + L E A FA +VL P F+AK+F+ GA+ Sbjct: 133 LEGKADIVMSDMASNTIGHKATDHMRTLLLCEQAFEFALKVLKPSCHFIAKIFRGGAETA 192 Query: 189 LVAQLKRDFATVRHVKPAASRQDSSERYVLA 219 L+ ++KR+F TV+H KP +SR +S+E Y++A Sbjct: 193 LLNKVKREFRTVKHFKPVSSRSESTEIYLVA 223