BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0046.0028.p (467 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1106 [Q] COG3670 Lignostilbene-alpha,beta-dioxygenase and rel... 335 1e-91 Rv0654 [Q] COG3670 Lignostilbene-alpha,beta-dioxygenase and rela... 203 8e-52 MT0683 [Q] COG3670 Lignostilbene-alpha,beta-dioxygenase and rela... 203 8e-52 RSp0212 [Q] COG3670 Lignostilbene-alpha,beta-dioxygenase and rel... 198 3e-50 all4284 [Q] COG3670 Lignostilbene-alpha,beta-dioxygenase and rel... 185 2e-46 >all1106 [Q] COG3670 Lignostilbene-alpha,beta-dioxygenase and related enzymes Nos Nostoc sp. PCC 7120 Length = 475 Score = 335 bits (859), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 185/457 (40%), Positives = 258/457 (56%), Gaps = 15/457 (3%) Query: 13 NLAPIPFEADAPFLKISGELPRDLNGTLYRNGPNPQFEAPGA-HWFVGDGMLHAFHLENG 71 N AP+ E LK+ GELP +L+G RNGPNPQ+ G HWF GDGMLH + NG Sbjct: 25 NFAPVHEEITTDTLKVIGELPPELSGMFVRNGPNPQWTPIGQYHWFDGDGMLHGVRISNG 84 Query: 72 RASYRNRWVRTPKWQAEHDAGRALFGGFGRKLPDAPATTTTDGGVANTNIIFHGGRLLAL 131 +A+YRNR+VRT +WQ EH+AG+A++ G L + P T NT +I+H G+LLAL Sbjct: 85 KATYRNRYVRTQRWQIEHEAGQAIWTG----LMEPPQTELPSRNTGNTALIWHAGQLLAL 140 Query: 132 EEGHLPTEIEPGTLNRLGYCDYKGAISGPFTAHPKIDPVTGEMVFFGYNAAGPLTPALSF 191 EG P I+ L +G Y +S FTAHPK+DPVTGEM+FFGY+ A P L + Sbjct: 141 WEGGAPYAIQVPDLASIGEYTYNNQLSSAFTAHPKVDPVTGEMMFFGYSFAPPY---LHY 197 Query: 192 GAVSSSGVVTRFERFEAPYASMVHDFIVTENHVLFPILPITGSMQRAMRGQAPYAWEPEK 251 VS++G + R + P M+HDF +T N+ +F LP+T S++R RG+ +E ++ Sbjct: 198 SVVSATGELVRTVPIDLPMGVMMHDFAITANYTIFMDLPLTFSVERMQRGEPMLMFESDR 257 Query: 252 GAYVGVMKRSGASKDIVWFRAETCYVFHVMNAWEDGNRIIADVMQFEEAPLFNHPDGSPT 311 + G++ R G + I WF A +CYVFH +NA+ED + ++ + PD S T Sbjct: 258 PSRFGILPRHGDNSQIRWFEAPSCYVFHTLNAYEDKDEVVLFACPMRSTTVLASPD-SQT 316 Query: 312 DPRKNRARYCRWTFDLSGNTDRFTQTYLDDLTGEFPRVDDRRAGFASNHGWYA-CANPDL 370 DP + R RW F L T + + LDD+ EFPR+++ G + +G+ + A + Sbjct: 317 DPEADIPRLHRWRFHL--KTGKVHEEMLDDVASEFPRINENFLGQPTQYGYTSRLAKGSI 374 Query: 371 PMFGALSGVVHVDGRGKRLGHYLLPAGDTISEPVFVERGADAAEGDGWLLAVVWRARENR 430 P+F G++ D + +Y G SE VFV R E DGWL+ V+ E Sbjct: 375 PLF---EGLIKYDLSNAKSQNYEYGQGRYGSEAVFVPRPGATVEDDGWLITYVYDTGEES 431 Query: 431 SDLAVFNAQDVEAGPTALVHLGHRVPDGFHGNWVGAE 467 S+L V NAQD+ + P A V L RVP GFHG WV E Sbjct: 432 SELVVINAQDINSEPIARVLLPQRVPYGFHGIWVTEE 468 >Rv0654 [Q] COG3670 Lignostilbene-alpha,beta-dioxygenase and related enzymes Mtu Mycobacterium tuberculosis H37Rv Length = 501 Score = 203 bits (516), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 161/510 (31%), Positives = 229/510 (44%), Gaps = 90/510 (17%) Query: 14 LAPIPFEADAPFLKISGELPRDLNGTLYRNGPNPQFEAPGA--HWFVGDGMLHAFHLENG 71 LAP+ E A L ++G +P L+G RNGPNP E A HWF GD M+H L +G Sbjct: 18 LAPVSTEVTATDLPVTGRIPEHLDGRYLRNGPNPVAEVDPATYHWFTGDAMVHGVALRDG 77 Query: 72 RAS-YRNRWVRTPKWQAEHDAGRALFGGFGRKLPDAP--ATTTTDGGVANTNIIFHGGRL 128 +A YRNRWVRTP A+ G + P T +GG NTN++ H GR Sbjct: 78 KARWYRNRWVRTP----------AVCAALGEPISARPHPRTGIIEGG-PNTNVLTHAGRT 126 Query: 129 LALEEGHLPTEIEPGTLNRLGYCDYKGAISGPFTAHPKIDPVTGEMVFFGYNAAGPLTPA 188 LAL E + L+ +G CD+ G + G +TAHP+ DP TGE+ Y+ A Sbjct: 127 LALVEAGVVNYELTDELDTVGPCDFDGTLHGGYTAHPQRDPHTGELHAVSYSFA--RGHR 184 Query: 189 LSFGAVSSSGVVTRFERFEAPYASMVHDFIVTENHVLFPILPIT---------------- 232 + + + + G R E + M+H F +T+N+V+ LP+T Sbjct: 185 VQYSVIGTDGHARRTVDIEVAGSPMMHSFSLTDNYVVIYDLPVTFDPMQVVPASVPRWLQ 244 Query: 233 --------------------GSMQRAMRGQA---PYAWEPEKGAYVGVMKRSGASKDIVW 269 ++ M+G + PYAW P A VGVM R G ++D+ W Sbjct: 245 RPARLVIQSVLGRVRIPDPIAALGNRMQGHSDRLPYAWNPSYPARVGVMPREGGNEDVRW 304 Query: 270 FRAETCYVFHVMNAWED----GNRIIADVMQFEEAPLFNHPDGSPTDPRKNRARYCRWTF 325 F E CYV+H +NA+ + ++ DV+++ +F+ P +R RWT Sbjct: 305 FDIEPCYVYHPLNAYSECRNGAEVLVLDVVRYSR--MFDRDRRGPGG--DSRPSLDRWTI 360 Query: 326 DLSGNTDRFTQTYLDDLTGEFPRVDDRRAG----FASNHGWY------ACANPDLPMFG- 374 +L+ T T DD EFPR+++ G FA G A A P++ Sbjct: 361 NLA--TGAVTAECRDDRAQEFPRINETLVGGPHRFAYTVGIEGGFLVGAGAALSTPLYKQ 418 Query: 375 -ALSGVVHVDGRGKRLGHYLLPAGDTISEPVFVERGADAAEGDGWLLAVVWRARENRSDL 433 ++G V L I E VFV + AE DG L+ W + L Sbjct: 419 DCVTGSSTVASLDPDL---------LIGEMVFVPNPSARAEDDGILMGYGWHRGRDEGQL 469 Query: 434 AVFNAQDVEAGPTALVHLGHRVPDGFHGNW 463 + +AQ +E+ A VHL RVP GFHGNW Sbjct: 470 LLLDAQTLES--IATVHLPQRVPMGFHGNW 497 >MT0683 [Q] COG3670 Lignostilbene-alpha,beta-dioxygenase and related enzymes MtC Mycobacterium tuberculosis CDC1551 Length = 501 Score = 203 bits (516), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 161/510 (31%), Positives = 229/510 (44%), Gaps = 90/510 (17%) Query: 14 LAPIPFEADAPFLKISGELPRDLNGTLYRNGPNPQFEAPGA--HWFVGDGMLHAFHLENG 71 LAP+ E A L ++G +P L+G RNGPNP E A HWF GD M+H L +G Sbjct: 18 LAPVSTEVTATDLPVTGRIPEHLDGRYLRNGPNPVAEVDPATYHWFTGDAMVHGVALRDG 77 Query: 72 RAS-YRNRWVRTPKWQAEHDAGRALFGGFGRKLPDAP--ATTTTDGGVANTNIIFHGGRL 128 +A YRNRWVRTP A+ G + P T +GG NTN++ H GR Sbjct: 78 KARWYRNRWVRTP----------AVCAALGEPISARPHPRTGIIEGG-PNTNVLTHAGRT 126 Query: 129 LALEEGHLPTEIEPGTLNRLGYCDYKGAISGPFTAHPKIDPVTGEMVFFGYNAAGPLTPA 188 LAL E + L+ +G CD+ G + G +TAHP+ DP TGE+ Y+ A Sbjct: 127 LALVEAGVVNYELTDELDTVGPCDFDGTLHGGYTAHPQRDPHTGELHAVSYSFA--RGHR 184 Query: 189 LSFGAVSSSGVVTRFERFEAPYASMVHDFIVTENHVLFPILPIT---------------- 232 + + + + G R E + M+H F +T+N+V+ LP+T Sbjct: 185 VQYSVIGTDGHARRTVDIEVAGSPMMHSFSLTDNYVVIYDLPVTFDPMQVVPASVPRWLQ 244 Query: 233 --------------------GSMQRAMRGQA---PYAWEPEKGAYVGVMKRSGASKDIVW 269 ++ M+G + PYAW P A VGVM R G ++D+ W Sbjct: 245 RPARLVIQSVLGRVRIPDPIAALGNRMQGHSDRLPYAWNPSYPARVGVMPREGGNEDVRW 304 Query: 270 FRAETCYVFHVMNAWED----GNRIIADVMQFEEAPLFNHPDGSPTDPRKNRARYCRWTF 325 F E CYV+H +NA+ + ++ DV+++ +F+ P +R RWT Sbjct: 305 FDIEPCYVYHPLNAYSECRNGAEVLVLDVVRYSR--MFDRDRRGPGG--DSRPSLDRWTI 360 Query: 326 DLSGNTDRFTQTYLDDLTGEFPRVDDRRAG----FASNHGWY------ACANPDLPMFG- 374 +L+ T T DD EFPR+++ G FA G A A P++ Sbjct: 361 NLA--TGAVTAECRDDRAQEFPRINETLVGGPHRFAYTVGIEGGFLVGAGAALSTPLYKQ 418 Query: 375 -ALSGVVHVDGRGKRLGHYLLPAGDTISEPVFVERGADAAEGDGWLLAVVWRARENRSDL 433 ++G V L I E VFV + AE DG L+ W + L Sbjct: 419 DCVTGSSTVASLDPDL---------LIGEMVFVPNPSARAEDDGILMGYGWHRGRDEGQL 469 Query: 434 AVFNAQDVEAGPTALVHLGHRVPDGFHGNW 463 + +AQ +E+ A VHL RVP GFHGNW Sbjct: 470 LLLDAQTLES--IATVHLPQRVPMGFHGNW 497 >RSp0212 [Q] COG3670 Lignostilbene-alpha,beta-dioxygenase and related enzymes Rso Ralstonia solanacearum Length = 520 Score = 198 bits (503), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 150/462 (32%), Positives = 226/462 (48%), Gaps = 40/462 (8%) Query: 26 LKISGELPRDLNGTLYRNGPNPQFEAPGA---HWFVGDGMLHAFHLEN----GRASYRNR 78 L + G P L+G+LYRNGP + + G H F GDGM+ + ++ R ++R Sbjct: 65 LTVEGRFPAALHGSLYRNGPV-RHQIGGLRYHHLFDGDGMIQRYDIDTRGARTRVTHRGV 123 Query: 79 WVRTPKWQAEHDAGRALFGGFGRKLPDAPATTTTDG-GVANTNIIFHGGRLLALEEGHLP 137 +VRT K+ A+ AGR + G F LP + D VANT+++ G LLAL EG Sbjct: 124 FVRTDKFVADTAAGRPVRGTFATLLPGMVPIDSPDAINVANTSVVMRGDELLALWEGGSA 183 Query: 138 TEIEPGTLNRLGYCDYKGAISG-PFTAHPKIDPVTGEMVFFGYNAAGPLTPALSFGAVSS 196 T ++P +L LG ++ +G PF+AHPK++ G + FG ++A L L+ V + Sbjct: 184 TRLDPDSLRTLGVKTWRADYAGMPFSAHPKVE-ADGTLRNFGVSSAAGL---LTVYCVGA 239 Query: 197 SGVVTRFERFEAPYASMVHDFIVTENHVLFPILPITGSMQRAMRGQA---PYAWEPEKGA 253 G + R + P +MVHDF VT+ H++F + ++RA G + + W PE G Sbjct: 240 DGALRRAQSVRVPQMAMVHDFAVTQRHLVFLLPSFIYDVERARDGASFLDAHVWRPELGM 299 Query: 254 YVGVMKRSGASKDIVWFRAETCYVFHVMNAWE--DGNRIIADVMQFEEAPLFNHP----- 306 V+ ++ A++ + WF +VFHV NAW DG RI D ++ ++A + Sbjct: 300 RALVLDKNDAAR-MRWFELPAGFVFHVGNAWSSPDGQRIHLDYVRSDDAAIVTTALRELM 358 Query: 307 DGSPTDPRKNRARYCRWTFDL-SGNTDRFTQTYLDDLTGEFPRVDDRRAGFASNHGWYAC 365 G P AR + T DL SG + Y EFP++D RR G + + Sbjct: 359 RGHIVHPAAP-ARLTQLTLDLRSGRAGQTATPY----AAEFPKIDTRRTGL-RHRALFCV 412 Query: 366 ANP------DLPMFGALSGVVHVDGRGKRLGHYLLPAGDTISEPVFVERGADAAEGDGWL 419 AN D P+ ++ D + Y + E +FV G +A EG+GWL Sbjct: 413 ANAVSRPGADSPI--GFDTLMRFDTESGHVQRYRFAQRALVEEHLFVPAGPNAPEGEGWL 470 Query: 420 LAVVWRARENRSDLAVFNAQDVEAGPTALVHLGHRVPDGFHG 461 L V+ R+ L VF+A++V AGP A+ VP GFHG Sbjct: 471 LGTVFDVDAGRTLLTVFDARNVSAGPVAVAAREGVVPQGFHG 512 >all4284 [Q] COG3670 Lignostilbene-alpha,beta-dioxygenase and related enzymes Nos Nostoc sp. PCC 7120 Length = 497 Score = 185 bits (470), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 138/462 (29%), Positives = 217/462 (46%), Gaps = 35/462 (7%) Query: 28 ISGELPRDLNGTLYRNGPNPQFEAPGA---HWFVGDGMLHAFHLENGRASYRNRWVRTPK 84 + GE+P +L GTL+RNGP + G H F GDGM+ NGRA YRN +V+T Sbjct: 39 VEGEIPTELQGTLFRNGPG-LLDIKGQSIHHPFDGDGMISRISFVNGRAHYRNSFVKTEG 97 Query: 85 WQAEHDAGRALFGG-FGRKLPDAPATTTTD---GGVANTNIIFHGGRLLALEEGHLPTEI 140 + AE +AG+ L G FG + P D +ANTN+I+ G +LLAL E P + Sbjct: 98 YLAEKNAGKILHRGVFGTQKPGGWLANIFDFKLKNIANTNVIYWGEKLLALWEAAEPHRL 157 Query: 141 EPGTLNRLGYCDYKGAISG--PFTAHPKIDPVT----GEMVFFGYNAAGPLTPALSFGAV 194 +P TL+ LG + G +S F AHP+ DP G ++ L+ ++ + Sbjct: 158 DPKTLDTLGKEYFHGVLSAGEAFGAHPRFDPSCEQDNGAPCLVNFSIKPGLSTTITIFEL 217 Query: 195 SSSGVVTRFERFEAPYASMVHDFIVTENHVLFPILPITGS---MQRAMRGQAPYAW-EPE 250 + +G V R P +HDF++T NH +F P++ + +RG +P Sbjct: 218 NVAGEVVRKHAHHVPGFCFIHDFVITPNHCIFFQNPVSFNPIPFALGLRGAGECIQVQPN 277 Query: 251 KGAYVGVMKR--SGASKDIVWFRAETCYVFHVMNAWEDGNRIIADVMQFEEAPL------ 302 + + V+ R K+I + ++FH +NA+ G I+ D + ++ P Sbjct: 278 QPTRIIVIPRFPQSGQKEIKTLEVGSGFIFHHVNAFVVGEEILVDSLCYDSLPEVEPESD 337 Query: 303 FNHPDGSPTDPRKNRARYCRWTFDLSGNTDRFTQTYLDDLTGEFPRVDDRRAGFASNHGW 362 F D P + W F L+ + ++ EFP + R G + + + Sbjct: 338 FRQVDFEAIAPGQ------LWRFYLNLKDGTVQKKLIESRCCEFPAIHPRNVGRSYRYLY 391 Query: 363 YACANPDLPMFGALSGVVHVD-GRGKRLGHYLLPAGDTISEPVFVERGADAAEGDGWLLA 421 + A+ L ++ +D G++ + P G T EP+FV R E DGW+LA Sbjct: 392 MSAAHAATGN-APLQALLKIDLESGEKQIWSVAPRGFT-GEPIFVPRPGSEKEDDGWVLA 449 Query: 422 VVWRARENRSDLAVFNAQDVEAGPTALVHLGHRVPDGFHGNW 463 +V+ A +RSD+ + +A D G A +HL H VP G HGN+ Sbjct: 450 LVYDAAHHRSDVVILDASDFTKGAIARLHLQHHVPYGLHGNF 491