BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0046.0022.p (555 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEI1016_1 [C] COG1951 Tartrate dehydratase alpha subunit/Fumara... 523 e-148 fumA_1 [C] COG1951 Tartrate dehydratase alpha subunit/Fumarate h... 416 e-115 STM4300_1 [C] COG1951 Tartrate dehydratase alpha subunit/Fumarat... 414 e-115 ZfumA_1 [C] COG1951 Tartrate dehydratase alpha subunit/Fumarate ... 414 e-115 fumB_1 [C] COG1951 Tartrate dehydratase alpha subunit/Fumarate h... 414 e-115 >BMEI1016_1 [C] COG1951 Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain Bme Brucella melitensis Length = 356 Score = 523 bits (1348), Expect = e-148, Method: Compositional matrix adjust. Identities = 248/345 (71%), Positives = 294/345 (85%), Gaps = 4/345 (1%) Query: 22 LFPLGADTTPYKKVTSEGVRVEKVLGKDMLVVSREALRALSEAAFGDINHYLRPGHLKQL 81 LFPLG D TPY+K+TS+ V V+ G+++L V E LR LSEAAF DINH LRPGHL+QL Sbjct: 9 LFPLGEDDTPYRKLTSDYVSVDTFKGQEVLTVDPEGLRLLSEAAFADINHLLRPGHLQQL 68 Query: 82 RSILEDKEASDNDKFVAFDFLKNANIAAGGVLPMCQDTGTAIIMGKKGCNVITDGDDEAA 141 IL+D EA+DND+FVA+D LKNANIAAGGVLPMCQDTGTAIIMGKKG V T+G D A Sbjct: 69 AKILQDPEATDNDRFVAYDLLKNANIAAGGVLPMCQDTGTAIIMGKKGRRVWTEGGDADA 128 Query: 142 LSEGARDAYLRRNLRYSQVAPLSMYEEKNTANNMPAQCEIYAEGQGDSAGAYKFMFMAKG 201 L G DAY ++NLRYSQ+APLSM+EEKNT NN+PAQ +IY EG+ AYKF+F+AKG Sbjct: 129 LGAGVLDAYNKKNLRYSQLAPLSMFEEKNTKNNLPAQIDIYEEGED----AYKFLFVAKG 184 Query: 202 GGSANKSFLFQATPSVLTRDRLLAFLKEKVLTLGTAACPPYHLAIVIGGTSAELCMKTVK 261 GGSANK+FL+Q TPS+LT DR++ FLK+K+L+LGTAACPPYHLAIVIGGTSAE+ +KTVK Sbjct: 185 GGSANKTFLYQGTPSLLTHDRMIDFLKDKILSLGTAACPPYHLAIVIGGTSAEMNLKTVK 244 Query: 262 LASARYLDALPTHGSADGNAFRDLEMEQEILKMTQSLGVGAQFGGKYFCHDVRVIRMPRH 321 LAS RYLD+LPT GS G+AFRDLEME EI K+TQSLGVGAQFGGKYFCHDVRVIR+PRH Sbjct: 245 LASTRYLDSLPTKGSETGHAFRDLEMEAEIHKLTQSLGVGAQFGGKYFCHDVRVIRLPRH 304 Query: 322 GASLPIGLGVSCSADRQVLGKITRDGVYLEELEHNPAQYLPAVEQ 366 GASLPIG+GVSCSADRQ GKIT+DG++LE+LE NPA+Y+P +++ Sbjct: 305 GASLPIGIGVSCSADRQAKGKITKDGIFLEQLEANPAKYMPDIDE 349 >fumA_1 [C] COG1951 Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain Eco Escherichia coli K12 Length = 358 Score = 416 bits (1068), Expect = e-115, Method: Compositional matrix adjust. Identities = 203/347 (58%), Positives = 259/347 (74%), Gaps = 4/347 (1%) Query: 16 PYKHTPLFPLGADTTPYKKVTSEGVRVEKVLGKDMLVVSREALRALSEAAFGDINHYLRP 75 P+ + FPL D T Y +TSE V V + G+++L V+ EAL L+ AF D + LRP Sbjct: 5 PFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAPEALTLLARQAFHDASFMLRP 64 Query: 76 GHLKQLRSILEDKEASDNDKFVAFDFLKNANIAAGGVLPMCQDTGTAIIMGKKGCNVITD 135 H +Q+ IL D EAS+NDK+VA FL+N++IAA GVLP CQDTGTAII+GKKG V T Sbjct: 65 AHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTG 124 Query: 136 GDDEAALSEGARDAYLRRNLRYSQVAPLSMYEEKNTANNMPAQCEIYAEGQGDSAGAYKF 195 G DEAAL+ G + Y+ NLRYSQ APL MY+E NT N+PAQ ++YA GD YKF Sbjct: 125 GGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAV-DGDE---YKF 180 Query: 196 MFMAKGGGSANKSFLFQATPSVLTRDRLLAFLKEKVLTLGTAACPPYHLAIVIGGTSAEL 255 + +AKGGGSANK++L+Q T ++LT +L +L EK+ TLGTAACPPYH+A VIGGTSAE Sbjct: 181 LCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET 240 Query: 256 CMKTVKLASARYLDALPTHGSADGNAFRDLEMEQEILKMTQSLGVGAQFGGKYFCHDVRV 315 +KTVKLASA+Y D LPT G+ G AFRD+E+E+E+L Q+LG+GAQFGGKYF HD+RV Sbjct: 241 NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRV 300 Query: 316 IRMPRHGASLPIGLGVSCSADRQVLGKITRDGVYLEELEHNPAQYLP 362 IR+PRHGAS P+G+GVSCSADR + KI R G+++E+LEHNP +Y+P Sbjct: 301 IRLPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIP 347 >STM4300_1 [C] COG1951 Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain Sty Salmonella typhimurium LT2 Length = 358 Score = 414 bits (1065), Expect = e-115, Method: Compositional matrix adjust. Identities = 202/340 (59%), Positives = 256/340 (75%), Gaps = 4/340 (1%) Query: 23 FPLGADTTPYKKVTSEGVRVEKVLGKDMLVVSREALRALSEAAFGDINHYLRPGHLKQLR 82 FP+G D T Y +TS+ V V + G+ +L V +AL L++ AF D + LRP H +Q+ Sbjct: 12 FPMGEDKTEYYLLTSDYVSVSEFNGESILNVEPQALTLLAQQAFHDASFMLRPEHQQQVA 71 Query: 83 SILEDKEASDNDKFVAFDFLKNANIAAGGVLPMCQDTGTAIIMGKKGCNVITDGDDEAAL 142 +IL D EAS+NDK+VA FL+N+ IAA G+LP CQDTGTAIIMGKKG V T G DEAAL Sbjct: 72 AILHDPEASENDKYVALQFLRNSEIAAKGILPTCQDTGTAIIMGKKGQRVWTGGGDEAAL 131 Query: 143 SEGARDAYLRRNLRYSQVAPLSMYEEKNTANNMPAQCEIYAEGQGDSAGAYKFMFMAKGG 202 S+G + Y+ NLRYSQ APL+MY+E NT +N+PAQ ++YA GD YKF+ +AKGG Sbjct: 132 SKGVFNTYIEDNLRYSQNAPLNMYKEVNTGSNLPAQIDLYAV-DGDE---YKFLCVAKGG 187 Query: 203 GSANKSFLFQATPSVLTRDRLLAFLKEKVLTLGTAACPPYHLAIVIGGTSAELCMKTVKL 262 GSANK++L+Q T ++LT +L FL EK+ TLGTAACPPYH+A VIGGTSAE +KTVKL Sbjct: 188 GSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAESTLKTVKL 247 Query: 263 ASARYLDALPTHGSADGNAFRDLEMEQEILKMTQSLGVGAQFGGKYFCHDVRVIRMPRHG 322 AS Y DALP G+ G AFRD +EQE+L+ Q LG+GAQFGGKYF HD+RVIR+PRHG Sbjct: 248 ASTHYYDALPAEGNEHGQAFRDPHLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHG 307 Query: 323 ASLPIGLGVSCSADRQVLGKITRDGVYLEELEHNPAQYLP 362 AS P+G+GVSCSADR + KI R+G+++E+LEHNP QY+P Sbjct: 308 ASCPVGMGVSCSADRNIKAKINREGIWIEKLEHNPGQYIP 347 >ZfumA_1 [C] COG1951 Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain EcZ Escherichia coli O157:H7 EDL933 Length = 358 Score = 414 bits (1065), Expect = e-115, Method: Compositional matrix adjust. Identities = 202/347 (58%), Positives = 259/347 (74%), Gaps = 4/347 (1%) Query: 16 PYKHTPLFPLGADTTPYKKVTSEGVRVEKVLGKDMLVVSREALRALSEAAFGDINHYLRP 75 P+ + FPL D T Y +TSE V + + G+++L V+ EAL L+ AF D + LRP Sbjct: 5 PFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAPEALTLLARQAFHDASFMLRP 64 Query: 76 GHLKQLRSILEDKEASDNDKFVAFDFLKNANIAAGGVLPMCQDTGTAIIMGKKGCNVITD 135 H +Q+ IL D EAS+NDK+VA FL+N++IAA GVLP CQDTGTAII+GKKG V T Sbjct: 65 AHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTG 124 Query: 136 GDDEAALSEGARDAYLRRNLRYSQVAPLSMYEEKNTANNMPAQCEIYAEGQGDSAGAYKF 195 G DEAAL+ G + Y+ NLRYSQ APL MY+E NT N+PAQ ++YA GD YKF Sbjct: 125 GGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAV-DGDE---YKF 180 Query: 196 MFMAKGGGSANKSFLFQATPSVLTRDRLLAFLKEKVLTLGTAACPPYHLAIVIGGTSAEL 255 + +AKGGGSANK++L+Q T ++LT +L +L EK+ TLGTAACPPYH+A VIGGTSAE Sbjct: 181 LCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET 240 Query: 256 CMKTVKLASARYLDALPTHGSADGNAFRDLEMEQEILKMTQSLGVGAQFGGKYFCHDVRV 315 +KTVKLASA+Y D LPT G+ G AFRD+E+E+E+L Q+LG+GAQFGGKYF HD+RV Sbjct: 241 NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRV 300 Query: 316 IRMPRHGASLPIGLGVSCSADRQVLGKITRDGVYLEELEHNPAQYLP 362 IR+PRHGAS P+G+GVSCSADR + KI R G+++E+LEHNP +Y+P Sbjct: 301 IRLPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIP 347 >fumB_1 [C] COG1951 Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain Eco Escherichia coli K12 Length = 358 Score = 414 bits (1064), Expect = e-115, Method: Compositional matrix adjust. Identities = 203/347 (58%), Positives = 256/347 (73%), Gaps = 4/347 (1%) Query: 16 PYKHTPLFPLGADTTPYKKVTSEGVRVEKVLGKDMLVVSREALRALSEAAFGDINHYLRP 75 P+ + FP+G D T Y +TS+ V V G+ +L V EAL L++ AF D + LRP Sbjct: 5 PFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASFMLRP 64 Query: 76 GHLKQLRSILEDKEASDNDKFVAFDFLKNANIAAGGVLPMCQDTGTAIIMGKKGCNVITD 135 H KQ+ +IL D EAS+NDK+VA FL+N+ IAA GVLP CQDTGTAII+GKKG V T Sbjct: 65 AHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTG 124 Query: 136 GDDEAALSEGARDAYLRRNLRYSQVAPLSMYEEKNTANNMPAQCEIYAEGQGDSAGAYKF 195 G DE LS+G + Y+ NLRYSQ A L MY+E NT N+PAQ ++YA GD YKF Sbjct: 125 GGDEETLSKGVYNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYAV-DGDE---YKF 180 Query: 196 MFMAKGGGSANKSFLFQATPSVLTRDRLLAFLKEKVLTLGTAACPPYHLAIVIGGTSAEL 255 + +AKGGGSANK++L+Q T ++LT +L FL EK+ TLGTAACPPYH+A VIGGTSAE Sbjct: 181 LCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAET 240 Query: 256 CMKTVKLASARYLDALPTHGSADGNAFRDLEMEQEILKMTQSLGVGAQFGGKYFCHDVRV 315 +KTVKLASA Y D LPT G+ G AFRD+++EQE+L+ Q LG+GAQFGGKYF HD+RV Sbjct: 241 NLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQFGGKYFAHDIRV 300 Query: 316 IRMPRHGASLPIGLGVSCSADRQVLGKITRDGVYLEELEHNPAQYLP 362 IR+PRHGAS P+G+GVSCSADR + KI R+G+++E+LEHNP QY+P Sbjct: 301 IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHNPGQYIP 347