BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0045.0004.p (415 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc3344 [E] COG0520 Selenocysteine lyase Atu Agrobacterium tumef... 566 e-161 SMc00533 [E] COG0520 Selenocysteine lyase Sme Sinorhizobium meli... 566 e-161 mlr0021 [E] COG0520 Selenocysteine lyase Mlo Mesorhizobium loti 551 e-157 BMEI1039 [E] COG0520 Selenocysteine lyase Bme Brucella melitensis 533 e-151 CC1860 [E] COG0520 Selenocysteine lyase Ccr Caulobacter vibrioides 501 e-141 >AGc3344 [E] COG0520 Selenocysteine lyase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 416 Score = 567 bits (1460), Expect = e-161, Method: Compositional matrix adjust. Identities = 265/404 (65%), Positives = 325/404 (80%) Query: 12 YEVNRLRADFPALSMTVYGRPLVYLDNAASAQKPKAVLDRMMQVYSSEYANVHRGLHYLA 71 Y+V +R DFP LS VYG+PL YLDN ASAQKP+ V+D + YS+EYANVHRGLH+L+ Sbjct: 13 YDVEAVRKDFPILSREVYGKPLTYLDNGASAQKPQVVIDAVSHAYSNEYANVHRGLHFLS 72 Query: 72 NAATEAYEGARAKLAQFVNARLNEEIIFTRSATEAINLVAQTFGRERVTPGDEIVLSMME 131 NAAT+AYE AR K+ +F+NA +EI+FT+S+TEAIN VA +G + GDEIV+S+ME Sbjct: 73 NAATDAYEAAREKVRRFLNAGSVDEIVFTKSSTEAINTVAYGYGMPVIGEGDEIVISIME 132 Query: 132 HHSNIVPWHFLRERHGAVIKWAPVDDDGNFLLDAFEQLLTRRTKMVAITQMSNALGTVLP 191 HHSNIVPWHF+RER GA + W PVDDDG F ++AFE+ LT RTK+VAIT MSNALGT+LP Sbjct: 133 HHSNIVPWHFIRERQGAKLVWVPVDDDGAFHIEAFEKSLTERTKLVAITHMSNALGTILP 192 Query: 192 VKEVVRIAHARGIPVLIDGSQGCVHLNADVCDIDCDFYVMTGHKLYGPTGIGVLYGKSEH 251 VKE+ RIAH RGIPVL+DGSQG VH+ DV DIDCD+YVMTGHKLYGP+GIGVLYGK+E Sbjct: 193 VKEICRIAHERGIPVLVDGSQGAVHMPVDVRDIDCDWYVMTGHKLYGPSGIGVLYGKTER 252 Query: 252 LALMPPFNGGGEMIREVSRTGVIYGDPPHRFEAGTPPIVEAVGLGAAVDYINSIGKKQIR 311 L M PF GGGEMI +VS V Y +PPHRFEAGTPPIV+A+GLG A+DY++++G+ I Sbjct: 253 LREMRPFQGGGEMILDVSEDNVTYNEPPHRFEAGTPPIVQAIGLGYALDYMDNLGRAAIA 312 Query: 312 EHESALLAYAQQQLADINSVRLIGTPAEKGPIISFDMKGAHAHDIATVIDRAGVAVRAGA 371 HE+ L AYA +L ++NS+R+IG +KG I SF+++G HAHD++ VIDR GVAVRAG Sbjct: 313 AHEADLAAYAATRLREVNSLRIIGNAPDKGGIFSFELEGIHAHDVSMVIDRRGVAVRAGT 372 Query: 372 HCAMPLLKRFGVLATCRASFGLYNTREEIDTLVGALIKARQLFS 415 HCAMPLLKRFGV +TCRASFGLYNTR E+D LV AL AR+ F+ Sbjct: 373 HCAMPLLKRFGVTSTCRASFGLYNTRAEVDRLVEALDYARKFFA 416 >SMc00533 [E] COG0520 Selenocysteine lyase Sme Sinorhizobium meliloti Length = 414 Score = 566 bits (1459), Expect = e-161, Method: Compositional matrix adjust. Identities = 266/412 (64%), Positives = 326/412 (79%) Query: 4 HPAMSNGRYEVNRLRADFPALSMTVYGRPLVYLDNAASAQKPKAVLDRMMQVYSSEYANV 63 HPA Y+V +R DFP LS TVYG+PLVYLDN ASAQKP+ V+D + YS+EYANV Sbjct: 3 HPAPPVAAYDVEAVRKDFPILSRTVYGKPLVYLDNGASAQKPQLVIDAVAHAYSNEYANV 62 Query: 64 HRGLHYLANAATEAYEGARAKLAQFVNARLNEEIIFTRSATEAINLVAQTFGRERVTPGD 123 HRGLH+L+NAATEAYE AR K+ +F+NA + IIFT+S+TEAIN VA +G ++ GD Sbjct: 63 HRGLHFLSNAATEAYEQAREKVRRFLNAPSVDNIIFTKSSTEAINTVAYGYGMPKLGEGD 122 Query: 124 EIVLSMMEHHSNIVPWHFLRERHGAVIKWAPVDDDGNFLLDAFEQLLTRRTKMVAITQMS 183 EIV+S+MEHHSNIVPWHF+RER GA + WAPVDDDG F ++ F + LT RTK++AIT MS Sbjct: 123 EIVISIMEHHSNIVPWHFIRERQGAKLVWAPVDDDGAFHIEDFVKCLTERTKLIAITHMS 182 Query: 184 NALGTVLPVKEVVRIAHARGIPVLIDGSQGCVHLNADVCDIDCDFYVMTGHKLYGPTGIG 243 NALGTV+PVKE+ RIA RGIPVL+DGSQG VH+ DV DIDCD+YVMTGHKLYGP+GIG Sbjct: 183 NALGTVVPVKEICRIARERGIPVLVDGSQGAVHMPVDVQDIDCDWYVMTGHKLYGPSGIG 242 Query: 244 VLYGKSEHLALMPPFNGGGEMIREVSRTGVIYGDPPHRFEAGTPPIVEAVGLGAAVDYIN 303 VLYGK+ L M PF GGGEMI EV+ V Y DPPHRFEAGTPPIV+A+GLG A+DY+ Sbjct: 243 VLYGKTGRLKEMRPFMGGGEMIEEVTEDRVTYNDPPHRFEAGTPPIVQAIGLGYALDYME 302 Query: 304 SIGKKQIREHESALLAYAQQQLADINSVRLIGTPAEKGPIISFDMKGAHAHDIATVIDRA 363 +G++ IR HE++L AYA+++L+ +NS+R+ G KG I SF++ G HAHD++ VIDRA Sbjct: 303 KVGREAIRAHEASLTAYARERLSSVNSLRVFGDAPGKGSIFSFEIAGIHAHDVSMVIDRA 362 Query: 364 GVAVRAGAHCAMPLLKRFGVLATCRASFGLYNTREEIDTLVGALIKARQLFS 415 G+AVRAG HCA PLLKRFGV +TCRASFGLYNTR E+D L AL AR+ F+ Sbjct: 363 GIAVRAGTHCAQPLLKRFGVTSTCRASFGLYNTRAEVDALADALEHARKFFA 414 >mlr0021 [E] COG0520 Selenocysteine lyase Mlo Mesorhizobium loti Length = 413 Score = 551 bits (1420), Expect = e-157, Method: Compositional matrix adjust. Identities = 260/403 (64%), Positives = 314/403 (77%) Query: 12 YEVNRLRADFPALSMTVYGRPLVYLDNAASAQKPKAVLDRMMQVYSSEYANVHRGLHYLA 71 Y+V +R DFP LS VYG+PLVYLDN ASAQKP+ VLD + YS EYANVHRGLH+L+ Sbjct: 10 YDVEAIRRDFPILSREVYGKPLVYLDNGASAQKPQVVLDTIQHAYSQEYANVHRGLHFLS 69 Query: 72 NAATEAYEGARAKLAQFVNARLNEEIIFTRSATEAINLVAQTFGRERVTPGDEIVLSMME 131 NAAT+AYE AR + +F+NA + I+FT + T AIN VA +G + GDEIVLS+ME Sbjct: 70 NAATDAYEKARETVRRFLNAPSTDNIVFTSNTTSAINTVAYGYGMPNIGEGDEIVLSIME 129 Query: 132 HHSNIVPWHFLRERHGAVIKWAPVDDDGNFLLDAFEQLLTRRTKMVAITQMSNALGTVLP 191 HHSNIVPWHF+RER GA + W PVDD G F ++ FE+ LT RTK+VAITQMSNALGTV P Sbjct: 130 HHSNIVPWHFIRERQGAKLVWVPVDDLGVFHIEEFEKRLTNRTKLVAITQMSNALGTVTP 189 Query: 192 VKEVVRIAHARGIPVLIDGSQGCVHLNADVCDIDCDFYVMTGHKLYGPTGIGVLYGKSEH 251 +KE+VRIAHARGIPVL+DGSQ VH+ DV D+DCDF+V TGHK+YGP+GIGVLYGK + Sbjct: 190 IKEIVRIAHARGIPVLVDGSQSAVHMPIDVQDLDCDFFVFTGHKVYGPSGIGVLYGKKDI 249 Query: 252 LALMPPFNGGGEMIREVSRTGVIYGDPPHRFEAGTPPIVEAVGLGAAVDYINSIGKKQIR 311 LA M PF GGGEMI EV+ V Y +PPHRFEAGTPPIV+A+GLGAA+DY+ IG+++I Sbjct: 250 LAGMRPFMGGGEMIEEVTEDIVTYNEPPHRFEAGTPPIVQAIGLGAALDYMEKIGRERIA 309 Query: 312 EHESALLAYAQQQLADINSVRLIGTPAEKGPIISFDMKGAHAHDIATVIDRAGVAVRAGA 371 HE L YA ++L INS+R+ G KG IISF+++G HAHD++ VIDR GVAVRAG Sbjct: 310 AHEEDLKTYAHERLRAINSLRIFGDAPGKGAIISFELQGIHAHDVSMVIDRQGVAVRAGT 369 Query: 372 HCAMPLLKRFGVLATCRASFGLYNTREEIDTLVGALIKARQLF 414 HCA PLLKRFGV +TCRASFG+YNTR E+D L AL KAR+ F Sbjct: 370 HCAQPLLKRFGVTSTCRASFGMYNTRAEVDALAEALEKARKFF 412 >BMEI1039 [E] COG0520 Selenocysteine lyase Bme Brucella melitensis Length = 436 Score = 533 bits (1372), Expect = e-151, Method: Compositional matrix adjust. Identities = 248/403 (61%), Positives = 316/403 (78%) Query: 12 YEVNRLRADFPALSMTVYGRPLVYLDNAASAQKPKAVLDRMMQVYSSEYANVHRGLHYLA 71 Y+V +R DFP LS V+G+ LVYLDN ASAQKP++V+D + Y++EYANVHRGLH+L+ Sbjct: 33 YDVEAIRRDFPILSRQVHGKTLVYLDNGASAQKPQSVIDAVTHAYANEYANVHRGLHFLS 92 Query: 72 NAATEAYEGARAKLAQFVNARLNEEIIFTRSATEAINLVAQTFGRERVTPGDEIVLSMME 131 NAAT+AYE +R + +F+NA +EI+FT++ATEAIN VA +G + GDEI+LS+ME Sbjct: 93 NAATDAYEKSRETVRRFLNAGSVDEIVFTKNATEAINTVAYGYGMPFIGEGDEILLSIME 152 Query: 132 HHSNIVPWHFLRERHGAVIKWAPVDDDGNFLLDAFEQLLTRRTKMVAITQMSNALGTVLP 191 HHSNIVPWHF+RER GA + + PVDD+G F ++ FE+ L+ RTK+VAIT MSN LGTV+P Sbjct: 153 HHSNIVPWHFIRERQGAKLVFTPVDDNGVFHIEEFEKRLSERTKLVAITHMSNTLGTVVP 212 Query: 192 VKEVVRIAHARGIPVLIDGSQGCVHLNADVCDIDCDFYVMTGHKLYGPTGIGVLYGKSEH 251 +K++V +AHARG+PVL+DGSQG VHL DV D+ CD+YV TGHK+YGP+GIGVLYG+++ Sbjct: 213 IKKIVELAHARGVPVLVDGSQGAVHLPVDVQDLGCDWYVFTGHKVYGPSGIGVLYGRAQM 272 Query: 252 LALMPPFNGGGEMIREVSRTGVIYGDPPHRFEAGTPPIVEAVGLGAAVDYINSIGKKQIR 311 L M PF GGGEMI EV+ V Y PPHRFEAGTPPIV+A+GLGAA++Y+ IG+ I Sbjct: 273 LEKMRPFQGGGEMIEEVTEENVTYNHPPHRFEAGTPPIVQAIGLGAALEYMEKIGRHAIL 332 Query: 312 EHESALLAYAQQQLADINSVRLIGTPAEKGPIISFDMKGAHAHDIATVIDRAGVAVRAGA 371 HE+ L YA ++L INS+R+ G +KG IISF ++G HAHD++ VIDRAGVAVRAG Sbjct: 333 AHEADLRDYAHERLGRINSLRIFGNAPDKGAIISFALEGIHAHDVSMVIDRAGVAVRAGT 392 Query: 372 HCAMPLLKRFGVLATCRASFGLYNTREEIDTLVGALIKARQLF 414 HCA PLLKRFGV +TCRASF LYNTR E+D L AL KAR+ F Sbjct: 393 HCAQPLLKRFGVTSTCRASFALYNTRAEVDALAEALEKARKFF 435 >CC1860 [E] COG0520 Selenocysteine lyase Ccr Caulobacter vibrioides Length = 408 Score = 501 bits (1291), Expect = e-141, Method: Compositional matrix adjust. Identities = 241/404 (59%), Positives = 303/404 (75%), Gaps = 2/404 (0%) Query: 12 YEVNRLRADFPALSMTVYGRPLVYLDNAASAQKPKAVLDRMMQVYSSEYANVHRGLHYLA 71 Y+V +RA FP L+ V+G+PL+YLD+AASAQKP AVLD MM + + YANVHRGLH LA Sbjct: 7 YDVAAIRAQFPILAREVHGKPLIYLDSAASAQKPDAVLDAMMGLARTSYANVHRGLHTLA 66 Query: 72 NAATEAYEGARAKLAQFVNARLNEEIIFTRSATEAINLVAQTFGRERVTPGDEIVLSMME 131 N TEAYE AR +A+F+NA +E+++T+SATEA+NLVA +FG + GDEIVLS ME Sbjct: 67 NETTEAYEKARETVARFINAE-RDEVVWTKSATEAVNLVASSFGLS-LNAGDEIVLSEME 124 Query: 132 HHSNIVPWHFLRERHGAVIKWAPVDDDGNFLLDAFEQLLTRRTKMVAITQMSNALGTVLP 191 HH+NIVPWHFLRER G V+K+ PV DDG ++A++ LL+ +TKMVA+T MSN LGTV Sbjct: 125 HHANIVPWHFLRERKGVVLKFIPVLDDGRLDMEAYKGLLSDKTKMVAVTHMSNVLGTVND 184 Query: 192 VKEVVRIAHARGIPVLIDGSQGCVHLNADVCDIDCDFYVMTGHKLYGPTGIGVLYGKSEH 251 V E+VR+AHA G PVL+DG QG VH DV +D DFYV +GHKLYGPTGIGVLYGK+E Sbjct: 185 VAEIVRLAHAAGAPVLLDGCQGVVHTKVDVKALDVDFYVFSGHKLYGPTGIGVLYGKAER 244 Query: 252 LALMPPFNGGGEMIREVSRTGVIYGDPPHRFEAGTPPIVEAVGLGAAVDYINSIGKKQIR 311 LA +PP+ GGGEMI V+ + Y DPPHRFEAGTP I+EAVGLGAA++++N + + I Sbjct: 245 LAALPPYQGGGEMIASVALDKITYADPPHRFEAGTPAILEAVGLGAAIEWLNGLDRDAIF 304 Query: 312 EHESALLAYAQQQLADINSVRLIGTPAEKGPIISFDMKGAHAHDIATVIDRAGVAVRAGA 371 HE AL ++L +N VR++G KG ++SF ++GAHAHDIA V+DR G+AVRAG Sbjct: 305 AHEHALYERVAERLRGVNGVRILGDAPGKGAVLSFTVEGAHAHDIAQVLDRYGIAVRAGT 364 Query: 372 HCAMPLLKRFGVLATCRASFGLYNTREEIDTLVGALIKARQLFS 415 HCA PL++RFGV ++ RASF LYNT E D V AL KAR FS Sbjct: 365 HCAEPLMRRFGVTSSARASFALYNTEAEADAFVDALAKARSFFS 408