BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0002.p (532 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF0687 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 297 3e-80 BS_yhfL [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 256 1e-67 PA2557 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 253 6e-67 CC0966 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 253 1e-66 BS_yngI [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 244 3e-64 >AF0687 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Afu Archaeoglobus fulgidus Length = 549 Score = 297 bits (761), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 173/525 (32%), Positives = 285/525 (54%), Gaps = 10/525 (1%) Query: 14 GRIGRVAIGDLLKRAARRFPDRIALT----DGNR-QVTFTELERDANRFANHLVQRGLKP 68 +I R I D L+R+ RR+P++ AL DG ++T++EL++ AN+ AN L+ G+K Sbjct: 23 NKIRRWVIADFLRRSGRRYPEKTALIFNHPDGREIRLTYSELDKMANKVANALLNLGIKK 82 Query: 69 GEKISTICNNSVEFVKALFGIHRAGLVWVPINTMLGPSDMDYILGHAEVRFAVIDDALHA 128 ++++ + +N++ V +FG +AG +++ +N ++ D+ Y + H+E + +++DAL Sbjct: 83 YDRVAILAHNTLHHVLTIFGCAKAGAIYLGLNYLMYGKDLAYCINHSEAKVLIVEDALFE 142 Query: 129 QADRRA-ALEARGMEMIAIDLTGNAGKTGLQEFNGLLEGQSDIEPDIEINDRDLAMIIYT 187 + + L+ + + G G+ + F+ +G+SD EPD +N D + YT Sbjct: 143 RIEPAMDQLKTVDTFLWSNQGVGVEGEGRWESFDDFFDGESDTEPDTILNIEDPVQMTYT 202 Query: 188 SGTTSRPKGAMHCHLAVVMAVMSNCIEMQLSRDDGITGQFPLFHCAGH-VLLLSYLSVGG 246 SGT S PK +H + +++ + ++ + + +D + P++HCA V ++ VGG Sbjct: 203 SGTESLPKCVVHTNESLIAQYVGCIVDGEYTANDIMLNTMPIYHCAQRDVFMMPIFYVGG 262 Query: 247 RMALMRGFDPVACMEAIVRDKLTVFVGLSLMYQAILDHPRRRDYDLSGLRCCIYTMAPMG 306 +M + +E I R K T+ ++ +L HP YDL LR C Y + Sbjct: 263 TNVMMTEPNLDLMLEYIDRYKPTMLFLAPTVWIGLLRHPDIDKYDLQSLRKCYYGASIFP 322 Query: 307 KPLLERAIADL-CPNFVLSSGQTEMYPATTMSRPEVQLDRFGNYWGESLIVNETAIMDDN 365 + L+ + L F GQTE+ P T++ E L+ L + D++ Sbjct: 323 EEPLKELMEKLPQARFFQYYGQTELAPYHTVANHEEILEYTKTAGRSGLNLESRLENDEH 382 Query: 366 GNLLPRGEIGELVHRGPNVMMGYYKDPRSTEEARKFGWHHTGDLALIDGNGEVLFLDRKK 425 + G GE+ RGP+VM+ YYKDP TEEA K GW H+GD+A+ +DRKK Sbjct: 383 EEIAEIGVPGEITARGPHVMLMYYKDPEKTEEAFKCGWFHSGDIAVKYRESYFEIVDRKK 442 Query: 426 DMIKSGGENVASVKIEETLLAHPAVQNAAVVGLPHPQWGEAVSAFVKLKPGAVADEAGIE 485 DMIK+GGENV+S ++EE + HP V AV+GLPH +W EAV+A V KPG + I Sbjct: 443 DMIKTGGENVSSREVEEVIYKHPDVWEVAVIGLPHEKWIEAVTAIVVPKPGKTINPEEII 502 Query: 486 AHCRKHLGGFQVPKLVRIL--EEMPMTATGKLRKVELRQQYSGHF 528 CRK L ++VPK V +L E++P T +GK+ K ELR+ + ++ Sbjct: 503 EFCRKELSPYKVPKGVIVLKPEDLPKTPSGKIMKRELRKIFKDYY 547 >BS_yhfL [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Bsu Bacillus subtilis Length = 513 Score = 256 bits (653), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 164/507 (32%), Positives = 252/507 (49%), Gaps = 15/507 (2%) Query: 25 LKRAARRFPDRIALTDGNRQVTFTELERDANRFANHLVQRGLKPGEKISTICNNSVEFVK 84 L+ A PD IA + +T+ EL RFA+ L + G++ G+ ++ + NS +F+ Sbjct: 7 LEETASEKPDSIACRFKDHMMTYQELNEYIQRFADGLQEAGMEKGDHLALLLGNSPDFII 66 Query: 85 ALFGIHRAGLVWVPINTMLGPSDMDYILGHAEVRFAV-IDDALHAQADRRAALEARGMEM 143 A FG +AG+V VPIN + P+++ Y+L + +V+ V + L +L +E+ Sbjct: 67 AFFGALKAGIVVVPINPLYTPTEIGYMLTNGDVKAIVGVSQLLPLYESMHESLPK--VEL 124 Query: 144 IAIDLTGNAG--------KTGLQEFNGLLEGQSDIEPDIEINDRDLAMIIYTSGTTSRPK 195 + + TG A + + F +L S + + E D A+I+YTSGTT +PK Sbjct: 125 VILCQTGEAEPEAADPEVRMKMTTFAKILRPTSAAKQNQEPVPDDTAVILYTSGTTGKPK 184 Query: 196 GAMHCHLAVVMAVMSNCIEMQLSRDDGITGQFPLFHCAGHVLLLSYLSVGGRMALMR-GF 254 GAM H + + + D + P+ H + ++ + G L+ F Sbjct: 185 GAMLTHQNLYSNANDVAGYLGMDERDNVVCALPMCHVFCLTVCMNAPLMSGATVLIEPQF 244 Query: 255 DPVACMEAIVRDKLTVFVGLSLMYQAILDHPRRRDYDLSGLRCCIYTMAPMGKPLLERAI 314 P + + + + + T+F G+ MY + H + D S +R CI A M LL Sbjct: 245 SPASVFKLVKQQQATIFAGVPTMYNYLFQHENGKKDDFSSIRLCISGGASMPVALLTAFE 304 Query: 315 ADLCPNFVLSSGQTEMYPATTMSRPEVQLDRFGNYWGESLIVNETAIMDDNGNLLPRGEI 374 + G +E P T + R G S++ E ++D G LP ++ Sbjct: 305 EKFGVTILEGYGLSEASPVTCFN--PFDRGRKPGSIGTSILHVENKVVDPLGRELPAHQV 362 Query: 375 GELVHRGPNVMMGYYKDPRSTEEARKFGWHHTGDLALIDGNGEVLFLDRKKDMIKSGGEN 434 GEL+ +GPNVM GYYK P TE A K GW +TGDLA D +G +DRKKDMI GG N Sbjct: 363 GELIVKGPNVMKGYYKMPMETEHALKDGWLYTGDLARRDEDGYFYIVDRKKDMIIVGGYN 422 Query: 435 VASVKIEETLLAHPAVQNAAVVGLPHPQWGEAVSAFVKLKPGAVADEAGIEAHCRKHLGG 494 V ++EE L +HP V+ A V+G+P PQ GEAV +V K V +E I HC KHL Sbjct: 423 VYPREVEEVLYSHPDVKEAVVIGVPDPQSGEAVKGYVVPKRSGVTEE-DIMQHCEKHLAK 481 Query: 495 FQVPKLVRILEEMPMTATGKLRKVELR 521 ++ P + L+++P ATGK+ + LR Sbjct: 482 YKRPAAITFLDDIPKNATGKMLRRALR 508 >PA2557 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Pae Pseudomonas aeruginosa Length = 564 Score = 253 bits (647), Expect = 6e-67, Method: Compositional matrix adjust. Identities = 171/541 (31%), Positives = 267/541 (49%), Gaps = 47/541 (8%) Query: 19 VAIGDLLKRAARRFPDRIALTDGNRQVTFT--ELERDANRFANHLVQRGLKPGEKISTIC 76 +GD R +R AL ++++ ++ +L + +A + GL+PGE++ Sbjct: 18 TTVGDAFDATVARHAEREALVVRHQRLRYSWRQLAERVDAYARAFIALGLRPGERLGIWA 77 Query: 77 NNSVEFVKALFGIHRAGLVWVPINTMLGPSDMDYILGHAEVRFAVIDDALHAQADRRAAL 136 N E+ F + G V V IN S+++Y L + + + DA +D A L Sbjct: 78 PNCAEWCITQFASAKVGAVLVNINPAYRSSELEYALKQSGCSWLICADAFKT-SDYHAML 136 Query: 137 ---------------------EARGMEMIA----------IDLTGNAGKTGLQEFNGLLE 165 E RG+ +A L G A G +E L + Sbjct: 137 GDLLPELARARPGELASERLPELRGVISLAERAPAGFLHWQGLPGLAAAVGAEE---LRQ 193 Query: 166 GQSDIEPDIEINDRDLAMIIYTSGTTSRPKGAMHCHLAVVMAVMSNCIEMQLSRDDGITG 225 Q+ ++ D IN I YTSGTT PKGA H ++ + L +D + Sbjct: 194 RQASLQFDEPIN------IQYTSGTTGFPKGATLSHYNILNNGYMVGESLGLGAEDRLVI 247 Query: 226 QFPLFHCAGHVL-LLSYLSVGGRMAL-MRGFDPVACMEAIVRDKLTVFVGLSLMYQAILD 283 PL+HC G V+ L ++ G M FD A + A+ ++ T G+ M+ A LD Sbjct: 248 PVPLYHCFGMVMGNLGCVTHGSTMIYPAPSFDAEATLLAVAEERATALYGVPTMFIAELD 307 Query: 284 HPRRRDYDLSGLRCCIYTMAPMGKPLLERAIADL-CPNFVLSSGQTEMYPATTMSRPEVQ 342 HPRRR++DLS LR I A ++ R I D+ ++ G TE P + + P+ Sbjct: 308 HPRRREFDLSSLRTGIMAGATCPIEVMRRVIGDMHMAEVQIAYGMTETSPVSLQTGPDDG 367 Query: 343 LDRFGNYWGESLIVNETAIMDDNGNLLPRGEIGELVHRGPNVMMGYYKDPRSTEEARKFG 402 L+ G + E+ I+D G ++PRGEIGEL RG +VM+GY+ DP++T EA Sbjct: 368 LELRVTTVGRTQPRLESKIVDQTGRVVPRGEIGELCTRGYSVMLGYWNDPQATAEAIDPA 427 Query: 403 -WHHTGDLALIDGNGEVLFLDRKKDMIKSGGENVASVKIEETLLAHPAVQNAAVVGLPHP 461 W HTGDLA++D +G V + R KDMI GGEN+ ++EE HPAV + V+G+P Sbjct: 428 RWMHTGDLAVMDDDGYVRIVGRSKDMIIRGGENIYPRELEEFFFTHPAVADVQVIGIPDE 487 Query: 462 QWGEAVSAFVKLKPGAVADEAGIEAHCRKHLGGFQVPKLVRILEEMPMTATGKLRKVELR 521 ++GE + A++KL PG AD+ + A C+ + F++P+ + ++E PMT TGK++K +R Sbjct: 488 RYGEEIVAWIKLHPGHHADDEQLRAFCKARIAHFKIPRHFKFVDEFPMTVTGKIQKFRMR 547 Query: 522 Q 522 + Sbjct: 548 E 548 >CC0966 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Ccr Caulobacter vibrioides Length = 530 Score = 253 bits (645), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 170/513 (33%), Positives = 247/513 (48%), Gaps = 13/513 (2%) Query: 21 IGDLLKRAARRFPDRIALTDGNRQVTFTELERDANRFANHLVQRGLKPGEKISTICNNSV 80 +GD+ + A PD +A + R+ TF +L+R N+ AN L+ GL G++I+ + NS Sbjct: 13 LGDVPRYHAEARPDAVAFSFEGRETTFAQLDRHTNQVANALLAAGLSTGDRIAYVGKNSD 72 Query: 81 EFVKALFGIHRAGLVWVPINTMLGPSDMDYILGHAEVRFAVIDDALHAQADRRAALEARG 140 + + L G +AG+V PI L ++ YI+G +E + + L D AA Sbjct: 73 HYFELLLGAAKAGVVTTPIGWRLAAPEIAYIVGDSEAKLVFVGRELIGHVDAVAAELTHR 132 Query: 141 MEMIAIDLTGNAGKTGLQEFNGLLEGQSDIEPDIEINDRDLAMIIYTSGTTSRPKGAMHC 200 +IA++ G AG Q F G + SD++P I D+A+ +YTSGTT RPKGAM Sbjct: 133 PVVIAMEAEG-AGD--YQTFEGWRDAASDVDPHKPIQISDIAIQLYTSGTTGRPKGAMLT 189 Query: 201 H---LAVVMAVMSNCIEM-QLSRDDGITGQFPLFHCAGHVLLLSYLSVGGRMALMRGFDP 256 H L + N +E Q D P+ H G L L G + + R FDP Sbjct: 190 HHNLLGMRREAAKNPLEWNQWGPSDVSLVAMPVAHIGGTGWGLVGLINGAKGVVAREFDP 249 Query: 257 VACMEAIVRDKLTVFVGLSLMYQAILDHPRRRDYDLSGLRCCIYTMAPMGKPLLERAIAD 316 ++ I +D+++ + Q ++ PR R+ D S L +Y AP+ LL + Sbjct: 250 TKVLDFIEKDRISKMFMVPAALQIVVRLPRAREVDYSRLTHILYGAAPIPLDLLRECMEV 309 Query: 317 LCPNFVLSSGQTEMYPATTMSRPEVQLDRFGNY----WGESLIVNETAIMDDNGNLLPRG 372 FV G TE T + P D GN G + E I+D+ G LP Sbjct: 310 FGCGFVQQYGMTETT-GTVVYLPPEDHDPAGNKRMRAAGLPMPGVELKIIDEAGKSLPPN 368 Query: 373 EIGELVHRGPNVMMGYYK-DPRSTEEARKFGWHHTGDLALIDGNGEVLFLDRKKDMIKSG 431 +GE+ R M GY+K D + + GW TGD +D +G + DR KDMI SG Sbjct: 369 TVGEVAVRSSANMAGYWKLDEATAKTMDADGWLRTGDAGYLDEDGYLFIHDRVKDMIISG 428 Query: 432 GENVASVKIEETLLAHPAVQNAAVVGLPHPQWGEAVSAFVKLKPGAVADEAGIEAHCRKH 491 GEN+ ++E + HP V AV+G+P +WGEAV A V KPG D I A R Sbjct: 429 GENIYPAEVESAVYGHPHVAEVAVIGVPDDKWGEAVKAVVAPKPGVTPDADDIIAFARTR 488 Query: 492 LGGFQVPKLVRILEEMPMTATGKLRKVELRQQY 524 + F+ PK V + +P A+GK+ + ELR Y Sbjct: 489 IAHFKAPKSVDFIPALPRNASGKILRRELRAPY 521 >BS_yngI [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Bsu Bacillus subtilis Length = 549 Score = 244 bits (624), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 168/533 (31%), Positives = 272/533 (51%), Gaps = 31/533 (5%) Query: 20 AIGDLLKRAARRFPDRIALTDGNRQVTFTELERDA--NRFANHLVQRGLKPGEKISTICN 77 IG LL++ A +PDR A+ +R + +T + D+ + A L++ G+ G+ ++ + Sbjct: 8 TIGRLLEQTADAYPDRDAVVYPDRNIRYTYAQFDSLCRQTAKGLMRMGIGKGDHVAIWAS 67 Query: 78 NSVEFVKALFGIHRAGLVWVPINTMLGPSDMDYILGHAEVRFAVIDDALHA--------- 128 N E++ F + G V V +NT ++DY+L ++ +I D+ Sbjct: 68 NISEWLAVQFATAKIGAVLVTVNTNYQAHELDYLLKQSDAAALIIMDSYRGTSYPDIVNS 127 Query: 129 -----QADRRAALEARGMEMI-AIDLTGNAGKTGLQEFNG---LLEGQSDIEPDIEINDR 179 Q + L++ + + GN +G+ ++ L + +D E + +N Sbjct: 128 LIPELQEAKPGQLKSERYPFLKTLIYIGNKRLSGMYHWDDTEILAKTVTDAELEERMNSL 187 Query: 180 DLAMII---YTSGTTSRPKGAMHCHLAVV--MAVMSNCIEMQLSRDDGITGQFPLFHCAG 234 D +I YTSGTT PKG M H V+ A ++ C M L+ D + P FHC G Sbjct: 188 DKDNVINMQYTSGTTGFPKGVMLTHFNVINNAANIAEC--MALTSQDRMCIPVPFFHCFG 245 Query: 235 HVL-LLSYLSVGGRMALMRGFDPVACMEAIVRDKLTVFVGLSLMYQAILDHPRRRDYDLS 293 VL +L+ +SVG M ++ FDPV ++ + ++K TV G+ M+ A L HP YDLS Sbjct: 246 CVLGVLACVSVGAAMIPVQEFDPVTVLKTVEKEKCTVLHGVPTMFIAELHHPDFDAYDLS 305 Query: 294 GLRCCIYTMAPMGKPLLERAIADL-CPNFVLSSGQTEMYPATTMSRPEVQLDRFGNYWGE 352 LR I +P +++ I + + ++ GQTE P T +R R G Sbjct: 306 TLRTGIMAGSPCPSEVMKAVIERMGMKDITIAYGQTEASPVITQTRANDSFIRRVETTGR 365 Query: 353 SLIVNETAIMDD-NGNLLPRGEIGELVHRGPNVMMGYYKDPRSTEEARKF-GWHHTGDLA 410 +L E I++ + RG GEL RG +VM GYYKD +T +A GW TGDLA Sbjct: 366 ALPHTEVKIVEPGTCQEVQRGMQGELCTRGYHVMKGYYKDKDATRKAINHDGWLFTGDLA 425 Query: 411 LIDGNGEVLFLDRKKDMIKSGGENVASVKIEETLLAHPAVQNAAVVGLPHPQWGEAVSAF 470 ++D +G R KDM+ GGEN+ +IEE L HPAV + VVG+P ++GE +A+ Sbjct: 426 VMDEDGYCRITGRLKDMLIRGGENIYPREIEEFLYQHPAVLDVQVVGVPDAKFGEEAAAW 485 Query: 471 VKLKPGAVADEAGIEAHCRKHLGGFQVPKLVRILEEMPMTATGKLRKVELRQQ 523 +KLK G ++A+C+ + ++P+ V ++ PMTA+GK++K +LR++ Sbjct: 486 IKLKDGKSVSPDELKAYCKGKIARHKIPRYVIFTDDYPMTASGKIQKYKLREK 538