BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0245.p (233 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa1978 [R] COG1011 Predicted hydrolase (HAD superfamily) Sme Si... 78 1e-14 RSc1362 [R] COG1011 Predicted hydrolase (HAD superfamily) Rso Ra... 76 7e-14 SMc00103 [R] COG1011 Predicted hydrolase (HAD superfamily) Sme S... 75 2e-13 mll7634 [R] COG1011 Predicted hydrolase (HAD superfamily) Mlo Me... 74 2e-13 PA0810 [R] COG1011 Predicted hydrolase (HAD superfamily) Pae Pse... 67 5e-11 >SMa1978 [R] COG1011 Predicted hydrolase (HAD superfamily) Sme Sinorhizobium meliloti Length = 246 Score = 78.2 bits (191), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 53/194 (27%), Positives = 93/194 (47%), Gaps = 7/194 (3%) Query: 15 FDQYGTVVDMQGGLTRIAAPFLEKKGWKGDPNSFVTWWRRTHFENSMIDALLHREHTPYR 74 FD YGT++ GL L K + ++F++ + R +E+ + R H ++ Sbjct: 25 FDCYGTLIQWDEGLLAAMERILAGKNRSIERDAFISVYDR--YEHRLER---ERPHRSFK 79 Query: 75 EIGHRAVAHVMDRAKIDYTMDDVRTLVGEIEKLVPFPEVPDALARLQRK-YKLVVLSNGD 133 + A+A M +D + DD L I ++ PFPEV L RL+ +KL ++SN D Sbjct: 80 NVSATALALAMGEFSLDVSPDDADILTSSISRMPPFPEVVATLTRLKAAGFKLAIISNTD 139 Query: 134 PDMLETAKQYHKVPFDRVISVTEANSFKPHVATYTKAAEIMGVKPAQVLFV-ANHAFDCI 192 ++ DRVI+ +A ++KP + A +G++ Q++ + A+ D Sbjct: 140 DAIIAGNVAQLGGSVDRVITAEQAGAYKPARQIFQHAWRELGIEKEQLVHICASPHLDLA 199 Query: 193 GAKSAGMRTAFIDR 206 A+ G RT ++DR Sbjct: 200 AARELGFRTIWVDR 213 >RSc1362 [R] COG1011 Predicted hydrolase (HAD superfamily) Rso Ralstonia solanacearum Length = 233 Score = 75.9 bits (185), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 59/208 (28%), Positives = 91/208 (43%), Gaps = 12/208 (5%) Query: 9 DVKICMFDQYGTVVDMQGGLTRIAAPFLEKKGWKGDPNSFVTWWRRTHFENSMIDALLH- 67 ++ +FD YGT+ D+ R F G + WR + + I +L Sbjct: 3 SIRAVVFDAYGTLFDVYSVAARAEQLF------PGKGEALSVLWRDRQIDYTRIRSLAGP 56 Query: 68 --REHTPYREIGHRAVAHVMDRAKIDYTMDDVRTLVGEIEKLVPFPEVPDALARLQR-KY 124 + P+ ++ A+ + R + TL+ E L FPE L +L+ Sbjct: 57 SGEHYKPFWDVTVDALRYACARLNLPLGNHAEATLMREYACLSAFPENVPVLRQLREMGL 116 Query: 125 KLVVLSNGDPDMLETAKQYHKVP--FDRVISVTEANSFKPHVATYTKAAEIMGVKPAQVL 182 L +LSNG+P MLE A + + FD V+SV +K A Y A GV AQ+L Sbjct: 117 PLGILSNGNPQMLEIAVKSAGMSGLFDHVLSVDAVRLYKTAPAAYALAPRAFGVPAAQIL 176 Query: 183 FVANHAFDCIGAKSAGMRTAFIDRRSRP 210 FV+++ +D GA G T +I+R P Sbjct: 177 FVSSNGWDACGATWHGFTTFWINRLGHP 204 >SMc00103 [R] COG1011 Predicted hydrolase (HAD superfamily) Sme Sinorhizobium meliloti Length = 220 Score = 74.7 bits (182), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 61/219 (27%), Positives = 100/219 (45%), Gaps = 13/219 (5%) Query: 14 MFDQYGTVVDMQGGLTRIAAPFLEKKGWKGDPNSFVTWWRRTHFENSMIDALLHREHTPY 73 +FD YGT+ D+ + R A E G D +F WR E S + +L+ + + Sbjct: 7 VFDAYGTLFDVHAAVRRHA----EAIG--PDGQAFSELWRAKQLEYSWVRSLMG-AYVDF 59 Query: 74 REIGHRAVAHVMDR-AKIDYTMDDVRTLVGEIEKLVPFPEVPDALARLQR-KYKLVVLSN 131 E+ +++ + R D + + L+ L +PEVP L L+ ++ +LSN Sbjct: 60 WELTEQSLDYAFQRFPSADPKLK--KPLLDAYWALDCYPEVPAVLKGLKELGARIAILSN 117 Query: 132 GDPDMLETAKQYHK--VPFDRVISVTEANSFKPHVATYTKAAEIMGVKPAQVLFVANHAF 189 G P ML +A + + D V SV +FK A Y + P V F +++ + Sbjct: 118 GSPAMLASAVKNAALDIIIDDVFSVDAVKAFKTAPAVYDLVTTSYRLYPQAVSFQSSNRW 177 Query: 190 DCIGAKSAGMRTAFIDRRSRPFGITPHQPDILVPSMKDL 228 D GA G RT +I+R P H P +++PS++ L Sbjct: 178 DIAGATKFGFRTVWINRADGPDEYKDHGPSLILPSLESL 216 >mll7634 [R] COG1011 Predicted hydrolase (HAD superfamily) Mlo Mesorhizobium loti Length = 219 Score = 74.3 bits (181), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 62/222 (27%), Positives = 106/222 (47%), Gaps = 13/222 (5%) Query: 14 MFDQYGTVVDMQGGLTRIAAPFLEKKGWKGDPNSFVTWWRRTHFENSMIDALLHREHTPY 73 +FD YGT+ D+ + R A ++ G G S + WR E S + +L+ + + Sbjct: 7 VFDAYGTLFDVHAAVRRHA----DRIGPDGQLLSEI--WRAKQLEYSWVRSLMG-SYADF 59 Query: 74 REIGHRAVAHVMDRAKIDYTMDDVRT-LVGEIEKLVPFPEVPDALARLQRK-YKLVVLSN 131 ++ +A+ + K+ +RT L+ +L +PEVP L L+ +L +LSN Sbjct: 60 WQLTEQALDFALR--KVPSADPGLRTKLLEAYWRLDCYPEVPAVLKALKASGARLALLSN 117 Query: 132 GDPDMLETAKQYHKVP--FDRVISVTEANSFKPHVATYTKAAEIMGVKPAQVLFVANHAF 189 G P+MLE A + + D + SV FK A Y A + P V F +++ + Sbjct: 118 GSPEMLEAAVKSAALDQVLDDIYSVDTVRRFKTDPAVYDMVATGWRLYPGAVSFQSSNRW 177 Query: 190 DCIGAKSAGMRTAFIDRRSRPFGITPHQPDILVPSMKDLADA 231 D GA G RT +I+R ++P P +++P+++ L A Sbjct: 178 DIAGATKFGFRTVWINRSNQPEEYRDFPPALILPTLESLVTA 219 >PA0810 [R] COG1011 Predicted hydrolase (HAD superfamily) Pae Pseudomonas aeruginosa Length = 233 Score = 66.6 bits (161), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 47/212 (22%), Positives = 94/212 (44%), Gaps = 11/212 (5%) Query: 10 VKICMFDQYGTVVDMQGGLTRIAAPFLEKKGWKGDPNSFVTWWRRTHFENSMIDALLHRE 69 ++ +FD +GT+VD + L + G WR+ + +D + Sbjct: 1 MRAILFDVFGTLVDWRSSLIEQFQALERELGGTLPCVELTDRWRQQY--KPAMDRV-RNG 57 Query: 70 HTPYREIG--HRAVAHVMDRAKIDYTMDD--VRTLVGEIEKLVPFPEVPDALARLQRKYK 125 P++ + HR + + +D+ ++ + G +L P+P+ + L+ Y Sbjct: 58 QAPWQHLDQLHRQSLEAL-AGEFGLALDEALLQRITGFWHRLRPWPDTLAGMHALKADYW 116 Query: 126 LVVLSNGDPDMLETAKQYHKVPFDRVISVTEANSFKPHVATYTKAAEIMGVKPAQVLFVA 185 L LSNG+ ++ ++ +P+D ++ +KP Y A ++ + P +V+ A Sbjct: 117 LAALSNGNTALMLDVARHAGLPWDMLLCADLFGHYKPDPQVYLGACRLLDLPPQEVMLCA 176 Query: 186 NHAFDCIGAKSAGMRTAFIDRRSRPFGITPHQ 217 H +D A++ G++TAFI +RP P Q Sbjct: 177 AHNYDLKAARALGLKTAFI---ARPLEYGPGQ 205