BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0170.p (780 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA5121_2 [M] COG0668 Small-conductance mechanosensitive channel ... 175 3e-43 RSc3068_2 [M] COG0668 Small-conductance mechanosensitive channel... 160 1e-38 SMa0630 [M] COG0668 Small-conductance mechanosensitive channel S... 157 1e-37 ECs0886_2 [M] COG0668 Small-conductance mechanosensitive channel... 154 1e-36 ZybiO_2 [M] COG0668 Small-conductance mechanosensitive channel E... 149 2e-35 >PA5121_2 [M] COG0668 Small-conductance mechanosensitive channel Pae Pseudomonas aeruginosa Length = 382 Score = 175 bits (444), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 108/302 (35%), Positives = 166/302 (54%), Gaps = 12/302 (3%) Query: 479 LEVWGVDAIVWFYGGQIGSRLISALVTIGIAALAAAVIWEVANALMDRQIDTLSREGHYA 538 L VWG+ I + G G ++ +V+ G L A +IW + + + +L G Sbjct: 83 LRVWGMSLIRYAEGE--GEQISMKVVSFGTTLLVAWLIWILTDTAIQH---SLGLGGKSR 137 Query: 539 RAARLRTFQPMLRTALFCMIVAVVGLTALSEIGVNVAPLLAGAGIVGIAIGFGSQKLVQD 598 R T P++R LF I + + AL+ +G+NV PLLAGAG++G+AIGFG+Q LV D Sbjct: 138 PNTRALTMLPLIRNVLFATIAVIALIVALANMGMNVTPLLAGAGVIGLAIGFGAQSLVAD 197 Query: 599 LITGLFLLIENTVQVGDNVTVSGLSGVVENVSIRTMRLRAGDGSVHVVPFSAVTTITNSS 658 LITGLF++IE+++ + D V V G G VE ++IRT+RLR DG VH +PFS + +I N S Sbjct: 198 LITGLFIIIEDSLSIDDYVDVGGHLGTVEGLTIRTVRLRDLDGVVHTIPFSEIKSIKNYS 257 Query: 659 RGAGNAAVSVNVSYKEDTDRAGEVLKDIVAQMRQETAYRAFIRGDLELWGVDKVDGSVVT 718 R G A V D A +++++ ++R + A + +EL GV+ D Sbjct: 258 RQFGYAMFRWPVPASMPIDDAVALVREVAMELRSDPAVYRNLWSPMELQGVESFDNGQAI 317 Query: 719 IVGQIRCTDSGRWAVQREFNRRMKRRFQEEGIEIASMGQTILMQLPR-----PSEPDTNA 773 + + + +W VQR FN R++RR + G+E+ SM + + +QL R +EPD Sbjct: 318 LRFRFKTAPIKQWEVQRAFNLRLRRRLDQAGLEL-SMPR-LNVQLSRAPKAKSAEPDEQG 375 Query: 774 QP 775 P Sbjct: 376 TP 377 >RSc3068_2 [M] COG0668 Small-conductance mechanosensitive channel Rso Ralstonia solanacearum Length = 299 Score = 160 bits (404), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 98/273 (35%), Positives = 155/273 (56%), Gaps = 9/273 (3%) Query: 496 GSRLISALVTIGIAALAAAVIWEVANALMDRQI-DTLSREGHYAR---AARLRTFQPMLR 551 G R+ ALV + + + W L+D I LS G AR + R RT P+LR Sbjct: 2 GRRIADALVGLIGTVFSTWLAW----ILLDTAILQALSPAGGRARLQPSTRARTILPLLR 57 Query: 552 TALFCMIVAVVGLTALSEIGVNVAPLLAGAGIVGIAIGFGSQKLVQDLITGLFLLIENTV 611 L +V G+ L+ +GVNV PL+AGAG++G+A+GFG+Q L QDLITG+F+L+E+T+ Sbjct: 58 NGLKVTLVVTAGIGVLANLGVNVTPLVAGAGVIGLAVGFGAQSLAQDLITGIFILMEDTI 117 Query: 612 QVGDNVTVSGLSGVVENVSIRTMRLRAGDGSVHVVPFSAVTTITNSSRGAGNAAVSVNVS 671 VGD V V +G V +++IRT+RLR G G++H +PFS + T+ N SR A V V+ Sbjct: 118 SVGDTVDVGVATGTVISLTIRTVRLRDGVGAIHSIPFSQIKTVRNLSRDYSFADFEVRVA 177 Query: 672 YKEDTDRAGEVLKDIVAQMRQETAYRAFIRGDLELWGVDKVDGSVVTIVGQIRCTDSGRW 731 D +A +++++ A+ + + + G E++G+D+ +G + + G+ + + Sbjct: 178 MDADPRQAIDLVREAAARTAGDVRFERILIGVPEVFGLDRFEGGAMIVKGRFKTRPQKQA 237 Query: 732 AVQREFNRRMKRRFQEEGIEIASMGQTILMQLP 764 V R FN +K F G+ +A M T+L P Sbjct: 238 DVLRAFNVVLKEGFDAAGVPLA-MPGTVLRPSP 269 >SMa0630 [M] COG0668 Small-conductance mechanosensitive channel Sme Sinorhizobium meliloti Length = 523 Score = 157 bits (396), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 88/266 (33%), Positives = 152/266 (57%), Gaps = 4/266 (1%) Query: 492 GGQIGSRLISALVTIGIAALAAAVIWEVANALMDRQIDTLSREG----HYARAARLRTFQ 547 G + G+ +I L+ IA L A ++W+++ AL+ ++ ++G AR+ RLRT Sbjct: 221 GSEPGAIVIPGLLNGIIALLVADLLWQMSKALIAYRMGLGPKDGSNVDELARSGRLRTLL 280 Query: 548 PMLRTALFCMIVAVVGLTALSEIGVNVAPLLAGAGIVGIAIGFGSQKLVQDLITGLFLLI 607 P+ R L I AV LT LS +GV +APL+AGAGI G+AIGFGSQ LV+D+++G+F ++ Sbjct: 281 PIFRNVLAVFIGAVTVLTILSGLGVQIAPLIAGAGIFGVAIGFGSQTLVKDVLSGVFYML 340 Query: 608 ENTVQVGDNVTVSGLSGVVENVSIRTMRLRAGDGSVHVVPFSAVTTITNSSRGAGNAAVS 667 ++ +VG+ + G VE+ S+R++RLR G + VPF + + N SR ++ Sbjct: 341 DDAFRVGEYIQSGSYKGTVESFSLRSVRLRHHRGPIFTVPFGELGAVQNMSRDWVIDKMT 400 Query: 668 VNVSYKEDTDRAGEVLKDIVAQMRQETAYRAFIRGDLELWGVDKVDGSVVTIVGQIRCTD 727 +NV+Y D D A +++K + ++ + + A L++ GVD + + ++ Sbjct: 401 LNVTYDADVDLARKLIKKVGQELAADPEFAADTIEPLKMQGVDSFGDFAIVLRMKLMTKP 460 Query: 728 SGRWAVQREFNRRMKRRFQEEGIEIA 753 ++ ++R +K+ F E GI+IA Sbjct: 461 GAQFTIKRRAFMMIKKAFDENGIKIA 486 >ECs0886_2 [M] COG0668 Small-conductance mechanosensitive channel Ecs Escherichia coli O157:H7 Length = 311 Score = 154 bits (388), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 104/301 (34%), Positives = 163/301 (54%), Gaps = 12/301 (3%) Query: 478 LLEVWGV-DAIVWFYGGQIGSRLISALVTIGIAALAAAVIWEVANALMDRQIDTLSREGH 536 LL WG+ D W G G + + L+ I + +AV W V +L++ ++ + G Sbjct: 12 LLSAWGLFDFWNWLQNGA-GQKTVDILIRIALILFFSAVGWTVLASLIENRLAS-DIHGR 69 Query: 537 YARAARLRTFQPMLRTALFCMIVAVVGLTALSEIGVNVAPLLAGAGIVGIAIGFGSQKLV 596 +AR RT + R AL +I + + LSEIGVN+APLLAGAG +G+AI FGSQ LV Sbjct: 70 PLPSARTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLV 129 Query: 597 QDLITGLFLLIENTVQVGDNVTVSGLSGVVENVSIRTMRLRAGDGSVHVVPFSAVTTITN 656 +D+ITG+F+ EN + GD VT+ L+G VE +SIR++ +R G+ H++P+S++TT N Sbjct: 130 KDIITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFAN 189 Query: 657 SSRGAGNAAVSVNVSYKEDTDRAGEVLKDIVAQMRQETAYRAFIRGDLELWGVDKVDGSV 716 RG G+ + +V ED D+A + LKD VA++ + R I G+ G+ + + Sbjct: 190 FVRGIGSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTA 249 Query: 717 VTIVGQIRCTDSGRWAVQREFNRRMKRRFQEEGIE--------IASMGQTILMQLPRPSE 768 T+ +W V+ + ++K+ F G+ + + G T LP P E Sbjct: 250 FTLRVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLP-PGE 308 Query: 769 P 769 P Sbjct: 309 P 309 >ZybiO_2 [M] COG0668 Small-conductance mechanosensitive channel EcZ Escherichia coli O157:H7 EDL933 Length = 291 Score = 149 bits (377), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 98/289 (33%), Positives = 159/289 (55%), Gaps = 5/289 (1%) Query: 489 WFYGGQIGSRLISALVTIGIAALAAAVIWEVANALMDRQIDTLSREGHYARAARLRTFQP 548 W G G + + L+ I + +AV W V +L++ ++ + G +AR RT Sbjct: 4 WLQNGA-GQKTVDILIRIALILFFSAVGWTVLASLIENRLAS-DIHGRPLPSARTRTLLT 61 Query: 549 MLRTALFCMIVAVVGLTALSEIGVNVAPLLAGAGIVGIAIGFGSQKLVQDLITGLFLLIE 608 + R AL +I + + LSEIGVN+APLLAGAG +G+AI FGSQ LV+D+ITG+F+ E Sbjct: 62 LFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGVFIQFE 121 Query: 609 NTVQVGDNVTVSGLSGVVENVSIRTMRLRAGDGSVHVVPFSAVTTITNSSRGAGNAAVSV 668 N + GD VT+ L+G VE +SIR++ +R G+ H++P+S++TT N RG G+ + Sbjct: 122 NGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGIGSVVANY 181 Query: 669 NVSYKEDTDRAGEVLKDIVAQMRQETAYRAFIRGDLELWGVDKVDGSVVTIVGQIRCTDS 728 +V ED D+A + LKD VA++ + R I G+ G+ + + T+ Sbjct: 182 DVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTLRVSFTTLPL 241 Query: 729 GRWAVQREFNRRMKRRFQEEGIEIASMGQTILMQLP--RPSEPDTNAQP 775 +W V+ + ++K+ F G+ A + ++ P P+EP +P Sbjct: 242 KQWTVRFALDSQVKKHFDLAGVR-APVQTYXVLPAPGATPAEPLPPGEP 289