BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0009.p (257 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2011 [E] COG0119 Isopropylmalate/homocitrate/citramalate synth... 264 2e-70 AGl2702 [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 238 1e-62 BMEI1926 [E] COG0119 Isopropylmalate/homocitrate/citramalate syn... 233 2e-61 SMb21125 [E] COG0119 Isopropylmalate/homocitrate/citramalate syn... 233 3e-61 RSc0261 [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 213 3e-55 >PA2011 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Pae Pseudomonas aeruginosa Length = 300 Score = 264 bits (674), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 125/231 (54%), Positives = 169/231 (73%) Query: 1 MSDQIKIIEMGPRDGLQNEKTPVSVEARIAFVEALVAAGLNTVEVGAFVSPKAIPQMASS 60 + +++++E+GPRDGLQNEK P+ V +I V+ L AAGL+ +EVG+FVSPK +PQMA S Sbjct: 3 LPKKVRLVEVGPRDGLQNEKQPIEVADKIRLVDDLSAAGLDYIEVGSFVSPKWVPQMAGS 62 Query: 61 EQVLRGVSHVKDAEFHVLVPNEKGYDAARAAGAQVVSVFAAASEGFSRANINCSVAESIE 120 +V G+ + L PN KG++AA +G + V+VFAAASE FS+ NINCS+ +S+E Sbjct: 63 AEVFAGIRQRPGVTYAALAPNLKGFEAALESGVKEVAVFAAASEAFSQRNINCSIKDSLE 122 Query: 121 RFKPVLARAKADGVKVRGYISCVLGCPFDGEIKPKAVADLAVTLWDLGCYEISLGDTIGV 180 RF PVL A+ V+VRGYISCVLGCP+DG++ P+ VA +A L +GCYE+SLGDTIGV Sbjct: 123 RFVPVLEAARQHQVRVRGYISCVLGCPYDGDVDPRQVAWVARELQQMGCYEVSLGDTIGV 182 Query: 181 GTPLKAKEMLRAVGSELPVGNLAMHFHDTYGQALANLYAGMEEGVRVIDAA 231 GT + ++ AV SE+P LA HFHDTYGQALAN+YA + EG+ V D++ Sbjct: 183 GTAGATRRLIEAVASEVPRERLAGHFHDTYGQALANIYASLLEGIAVFDSS 233 >AGl2702 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 321 Score = 238 bits (607), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 117/239 (48%), Positives = 159/239 (66%), Gaps = 4/239 (1%) Query: 4 QIKIIEMGPRDGLQNEKTPVSVEARIAFVEALVAAGLNTVEVGAFVSPKAIPQMASSEQV 63 +KI+E+G RDGLQNE VS +I +E L AAGL VE GAFVSP+ +PQMA S +V Sbjct: 19 NVKIVEVGARDGLQNESAEVSTAIKIELIERLAAAGLPAVEAGAFVSPRKVPQMADSREV 78 Query: 64 LRGVSHVKDAEFHVLVPNEKGYDAARAAGAQVVSVFAAASEGFSRANINCSVAESIERFK 123 +G+ + LVPN KG++AA AG ++VF +AS+GFSR NI CS AES+ER + Sbjct: 79 FQGLKRWPGTAYAALVPNMKGFEAAIEAGVTEIAVFVSASDGFSRHNIGCSRAESLERLR 138 Query: 124 PVLARAKADGVKVRGYISCVLGCPFDGEIKPKAVADLAVTLWDLGCYEISLGDTIGVGTP 183 V A +++RGY+SC+ GCP+DG + P VA +A L LGCYEISLGDTIGVGT Sbjct: 139 DVAEAAADRNIRMRGYVSCIAGCPYDGAVAPDDVAAMAEALVALGCYEISLGDTIGVGTA 198 Query: 184 LKAKEMLRAVGSELPVGNLAMHFHDTYGQALANLYAGMEEGVRVIDAA----AGXPTPP 238 + ++++ V + +P +AMHFHDTYGQ +AN+ A ++EG+ V D++ G P P Sbjct: 199 GQIRDVIERVATGIPRDQIAMHFHDTYGQGVANVLASLQEGIVVFDSSVAGLGGCPFAP 257 >BMEI1926 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Bme Brucella melitensis Length = 287 Score = 233 bits (595), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 117/233 (50%), Positives = 160/233 (68%) Query: 1 MSDQIKIIEMGPRDGLQNEKTPVSVEARIAFVEALVAAGLNTVEVGAFVSPKAIPQMASS 60 M++ ++I+EM RDGLQNEK V +IA + L G +E +FVSPK +PQ+A S Sbjct: 1 MAEHVEIVEMAARDGLQNEKRFVPTADKIALINRLSDCGYARIEATSFVSPKWVPQLADS 60 Query: 61 EQVLRGVSHVKDAEFHVLVPNEKGYDAARAAGAQVVSVFAAASEGFSRANINCSVAESIE 120 +V+ G+ + VLVPN KGY+AA AA A ++VF +ASEGFS+ANINC++AESIE Sbjct: 61 REVMAGIRRADGVRYSVLVPNMKGYEAAAAAHADEIAVFISASEGFSKANINCTIAESIE 120 Query: 121 RFKPVLARAKADGVKVRGYISCVLGCPFDGEIKPKAVADLAVTLWDLGCYEISLGDTIGV 180 R PV+ A DG+ +RGY+SCV+ CP+DG + P+AVA + L+ LGC+E+SLGDTIG Sbjct: 121 RLSPVIGAAINDGLAIRGYVSCVVECPYDGPVTPQAVASVTEQLFSLGCHEVSLGDTIGR 180 Query: 181 GTPLKAKEMLRAVGSELPVGNLAMHFHDTYGQALANLYAGMEEGVRVIDAAAG 233 GTP ML AV + P +LA H+HDT G+AL N+ +E+G+RV DA+ G Sbjct: 181 GTPDTVAAMLDAVLAIAPAHSLAGHYHDTGGRALDNIRVSLEKGLRVFDASVG 233 >SMb21125 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Sme Sinorhizobium meliloti Length = 289 Score = 233 bits (595), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 115/231 (49%), Positives = 154/231 (66%) Query: 3 DQIKIIEMGPRDGLQNEKTPVSVEARIAFVEALVAAGLNTVEVGAFVSPKAIPQMASSEQ 62 +++ I+E+ PRDGLQNE V+ E +I V+ L G +EV +FVSP+ +PQ+A + Sbjct: 7 ERVTIVEVAPRDGLQNESRLVATEDKIRLVDLLADCGYERIEVTSFVSPRWVPQLADAPA 66 Query: 63 VLRGVSHVKDAEFHVLVPNEKGYDAARAAGAQVVSVFAAASEGFSRANINCSVAESIERF 122 V+ G+ + L PN +G++AA AAGA V++FA+ASE FS NINCS+AESIERF Sbjct: 67 VMAGIVRRPGTRYAALTPNMRGFEAALAAGADEVAIFASASESFSERNINCSIAESIERF 126 Query: 123 KPVLARAKADGVKVRGYISCVLGCPFDGEIKPKAVADLAVTLWDLGCYEISLGDTIGVGT 182 +PV ++ GV +RGY+SCV+ CP++G I P A +A L DLGCYEISLGDTIG GT Sbjct: 127 RPVAEASRHRGVPLRGYVSCVVECPYEGAIVPAETARVARLLADLGCYEISLGDTIGRGT 186 Query: 183 PLKAKEMLRAVGSELPVGNLAMHFHDTYGQALANLYAGMEEGVRVIDAAAG 233 P ML A E+ LA HFHDT G+AL N+ +E G+RV DA+AG Sbjct: 187 PEAVDAMLAAALREIDAPKLAGHFHDTSGRALENIAVALERGIRVFDASAG 237 >RSc0261 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Rso Ralstonia solanacearum Length = 309 Score = 213 bits (543), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 114/227 (50%), Positives = 151/227 (66%) Query: 5 IKIIEMGPRDGLQNEKTPVSVEARIAFVEALVAAGLNTVEVGAFVSPKAIPQMASSEQVL 64 +K++E+GPRDGLQNEK+PVS EA++ V L AG VE +FVSP+ +PQMA V+ Sbjct: 7 VKVVEVGPRDGLQNEKSPVSTEAKVELVNRLSDAGFVNVEAASFVSPRWVPQMADGADVM 66 Query: 65 RGVSHVKDAEFHVLVPNEKGYDAARAAGAQVVSVFAAASEGFSRANINCSVAESIERFKP 124 + + L PN KG++AA AA A V +F+AASE FS+ NINCS+AESIERF P Sbjct: 67 ARIQRRPGTLYSALTPNMKGFEAAAAALADEVVIFSAASEAFSQKNINCSIAESIERFVP 126 Query: 125 VLARAKADGVKVRGYISCVLGCPFDGEIKPKAVADLAVTLWDLGCYEISLGDTIGVGTPL 184 V AK G+++RG ISC LGCP+ GE+ +V D+ L LGC EI + DTIGVGT Sbjct: 127 VARAAKDAGIRLRGSISCALGCPYQGEVPVASVVDVVKRLAALGCDEIDIADTIGVGTAG 186 Query: 185 KAKEMLRAVGSELPVGNLAMHFHDTYGQALANLYAGMEEGVRVIDAA 231 + K ++ A + P+ L+ HFHDTYGQALAN+ A +E GV + A+ Sbjct: 187 RVKAVMEAAAAAFPLDRLSGHFHDTYGQALANILASLEVGVAIFHAS 233