BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0002.p (342 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll3064 Non COG 270 5e-72 AGl3497 [P] COG0025 NhaP-type Na+/H+ and K+/H+ antiporters Atu A... 104 2e-22 BS_yvgP [P] COG0025 NhaP-type Na+/H+ and K+/H+ antiporters Bsu B... 91 5e-18 PA3660 [P] COG0025 NhaP-type Na+/H+ and K+/H+ antiporters Pae Ps... 90 8e-18 YPO3630 [P] COG0025 NhaP-type Na+/H+ and K+/H+ antiporters Ype Y... 86 1e-16 >mll3064 Non COG Length = 517 Score = 270 bits (689), Expect = 5e-72, Method: Compositional matrix adjust. Identities = 160/340 (47%), Positives = 215/340 (63%), Gaps = 19/340 (5%) Query: 7 VPSITIALVGSLAAGFLFAQVWMMITRRITEAPSAIITQFGGTFMVWIIAEHLGLSGILT 66 +P I ++ +GSL AG+L ++ ++ RI +A S+ + QF GTF VWI+A+ LGLS I+T Sbjct: 177 IPVILLSTIGSLIAGYLLGRLSLLTLTRIEDAASSTVVQFAGTFGVWILADKLGLSAIIT 236 Query: 67 IIAYAITISRTAPARTSARLRVSSYAVWETVVFVLNVLAFMLIGLQLRPIWSGLDDEVRW 126 I+ YAITI+R AP R SAR RVS+Y+VWE+ VFVLNVLAF+L+GLQ R I L E + Sbjct: 237 IVVYAITIARRAPRRMSARRRVSTYSVWESAVFVLNVLAFVLMGLQARSIVGRLSGEGQG 296 Query: 127 KYCSFAAAILAVVILARILWVMPYGALLRTL-------KARNLLPAHVVDAVPTLKRGFI 179 + FAA +L VVI+AR++WV Y A++R K R+L PT + Sbjct: 297 EAFLFAATVLVVVIVARLVWVASYVAIIRWFARVGSEDKKRDL---------PTFGGAVL 347 Query: 180 VSWCGMRGIVTLAAAFALPEWFPYRDLILLTAFAVVLGSLVIQGLTLRPLILALNFADDD 239 V WCGMRG+VTL A ALP FP RD I+L AFAVVLG+LV+QG+TL+PL+ L+F D Sbjct: 348 VGWCGMRGLVTLVVAIALPAGFPGRDPIVLAAFAVVLGTLVLQGMTLKPLLRILDFDPDR 407 Query: 240 PVGREAARARGVVFRAALDEIDADPSEEAEILRLEYRAVLLRAENDPDGGLTSRELPSDP 299 + E A+AR V +AALD + S A ++R +Y A + AEN D + D Sbjct: 408 TIDNEVAQARVAVMQAALDVLSRKTSAAAAVVREQYEAQRVIAENPEDAQAATE---YDR 464 Query: 300 LRRRAISAARQALLNLRRTEAIGDDAFHLVEEELDRAELS 339 LR AI+ R L LR IGD+A+H +EEE+D AEL+ Sbjct: 465 LRLYAINRQRDTLEELRSNGTIGDEAYHRLEEEIDWAELA 504 >AGl3497 [P] COG0025 NhaP-type Na+/H+ and K+/H+ antiporters Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 550 Score = 104 bits (260), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 95/341 (27%), Positives = 151/341 (44%), Gaps = 25/341 (7%) Query: 15 VGSLAAGFLFAQVWMMITRRITEAPSAIITQFGGTFMVWIIAEHLGLSGILTIIAYAITI 74 VG A G +W+ R + + I ++ +I+AE SG++ + + Sbjct: 213 VGGAAVGAFVGLLWVKTVRHLGDEYLMIAATAVLSWAAYILAEQAQTSGVIATVTAGLIA 272 Query: 75 SRTAPARTSARLRVSSYAVWETVVFVLNVLAFMLIGLQLRPIW--SGLDDEVRWKYCSFA 132 S SA R+ + W +VF++ + F+LIGL LR + G + A Sbjct: 273 SWHQHTVLSAATRMRGTSFWNVLVFLMEAMVFILIGLSLRDVVERGGGFGSIASSMALPA 332 Query: 133 AAILAVVILARILWVMPYGALLRTLKARNLLPAHVVDAVPTLKRGFIVSWCGMRGIVTLA 192 A+L ++ AR +W+ ++R L AH P + W GMRG+VTLA Sbjct: 333 LAVLVTLVAARFVWIFASDLVIRLFHGLKL--AHSKPLGPGAVTA--LGWAGMRGVVTLA 388 Query: 193 AAFALPEWFPYRDLILLTAFAVVLGSLVIQGLTLRPLILALNFADDDP-----VGREAAR 247 A +LPE FP RD IL+ AFAV++G+++IQG TL +I +P EA Sbjct: 389 LALSLPEDFPGRDFILVMAFAVIMGTVLIQGTTLGRIIAWAGLGGIEPERAPMTMSEAEA 448 Query: 248 ARGVVFRAALDEIDADPSEEAEILRLEYRAVLLRAENDPDGGLTSRELPS--DPLRR--- 302 + V + + D + E L + +L R E+ + E PL Sbjct: 449 SMAQVQFTTVQGLAYDENGE-----LIHPQLLKRYEHRLAASVKYAESTEKYSPLLHAHF 503 Query: 303 ----RAISAARQALLNLRRTEAIGDDAFHLVEEELDRAELS 339 +A++A R+ L+ LRR I D+ +E +LD EL+ Sbjct: 504 DVVLKAVAAGRKELIRLRREGQIDDETLRELEHDLDLEELT 544 >BS_yvgP [P] COG0025 NhaP-type Na+/H+ and K+/H+ antiporters Bsu Bacillus subtilis Length = 670 Score = 90.5 bits (223), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 80/308 (25%), Positives = 150/308 (48%), Gaps = 31/308 (10%) Query: 50 FMVWIIAEHLGLSGILTIIAYAIT--ISRTAPARTSARLRVSSYAVWETVVFVLNVLAFM 107 F++++ AE +G+SGIL ++A IT + + T +L++ S + W ++F+LN L F+ Sbjct: 228 FVIYLAAEEIGVSGILAVVAGGITHAVEQDRLESTMIKLQIVSSSTWNIILFILNGLVFV 287 Query: 108 LIGLQLRPIWSGLDDEV---RWKYCSFAAAILAVVILARILWVMPY--GALLRTLKARNL 162 ++G Q+ + S + ++ K + I ++L R LWV+ + G K +N+ Sbjct: 288 ILGTQIPDVISVIFNDTAISNMKVIGYILVITFTLMLLRFLWVLFFWNGKWFFN-KDQNI 346 Query: 163 LPAHVVDAVPTLKRGFIVSWCGMRGIVTLAAAFALPEW------FPYRDLILLTAFAVVL 216 P L+ ++S G+RG VTLA +F++P + FP R+LIL A V+L Sbjct: 347 YK-------PGLRSTLLISISGVRGAVTLAGSFSIPYFLEDGTPFPERNLILFLAAGVIL 399 Query: 217 GSLVIQGLTLRPLILALNFADDDPVGREAARARGVVFRAALDEIDADPSEEAEILRL--- 273 +LVI + L IL +D+ ++ AR + + AL I D +E + L Sbjct: 400 CTLVIATVVLP--ILTEKEEEDEERNKKLLTARRKLIKTALQTIKEDMNETNKTASLAVI 457 Query: 274 -EYRAVL--LRAENDPDGGLTSRELPSDPLRRRAISAARQALLNLRRTEAIGDDAFHLVE 330 EY + LR + + +R + + A ++AL+ + I ++ ++++ Sbjct: 458 AEYNEKMKNLRFQQYTSSNRIKKH--ERKVRAQGVKAEQEALMKMLERGDIPEETANVLQ 515 Query: 331 EELDRAEL 338 E + E+ Sbjct: 516 ERFNELEI 523 >PA3660 [P] COG0025 NhaP-type Na+/H+ and K+/H+ antiporters Pae Pseudomonas aeruginosa Length = 581 Score = 89.7 bits (221), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 108/361 (29%), Positives = 164/361 (45%), Gaps = 42/361 (11%) Query: 15 VGSLAAGFLFA------QVWMMITRRITEAPSAIITQFGGTFMVWIIAEHLGLSGILTII 68 VG LA G + + WM I+R + + ++ F +++AEHLG+SGIL+ + Sbjct: 214 VGGLAIGVFLSWSLGRIRAWM-ISRGWDDPATHVVLMLLLPFASYMVAEHLGVSGILSAV 272 Query: 69 AYAITIS--RTAPARTSARLRVSSYAVWETVVFVLNVLAFMLIGLQLRPIWSGLDD---E 123 A + S P +TS RL S VW + F N + F+L+GLQL I + + + Sbjct: 273 AAGMMQSWVDLLPRQTSTRLLNRS--VWSMLEFAFNGVVFLLLGLQLPDIMKSVANHHGD 330 Query: 124 VRWKYC---SFAAAILAVVILARILWVMPYGAL-LRTLKARNLLPAHVVDAVPTLKRGFI 179 + W+ ++ AAI AV++L R WV Y L LR + R P + + + + Sbjct: 331 LLWRSSLLLAYVAAITAVLMLIRYGWVYGYWKLSLRIDRWRGKSPPG-LSGISLRRLSAL 389 Query: 180 VSWCGMRGIVTLAAAFALP------EWFPYRDLILLTAFAVVLGSLV---IQGLTLRPLI 230 + G+RG VTLAA ++P FP RDLI+ A +V+L SL+ I L + Sbjct: 390 SALSGVRGAVTLAAVLSVPMSLMDGSAFPQRDLIIFIATSVILISLIGATIGLPLLLRGL 449 Query: 231 LALNFADDDPVGREAARARGVVFRAALDEID---------ADPSEEAEI---LRLEYRAV 278 A N + REA R AL+ + A+ S AE + EYR Sbjct: 450 PASNNGAHERERREAWRKTAEAAIRALESEELPQVEGNDAANTSRVAEARAQIMAEYRQS 509 Query: 279 LLRAENDPDGGLTSR--ELPSDPLRRRAISAARQALLNLRRTEAIGDDAFHLVEEELDRA 336 L A + ++ E+ LR +A+ A R L RR I D+ + ELD+ Sbjct: 510 LDTAPTTDEARDQAQALEIADKALRLKALRAQRLELYRQRRNNEIDDETLGDILRELDQQ 569 Query: 337 E 337 E Sbjct: 570 E 570 >YPO3630 [P] COG0025 NhaP-type Na+/H+ and K+/H+ antiporters Ype Yersinia pestis Length = 549 Score = 85.9 bits (211), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 96/364 (26%), Positives = 171/364 (46%), Gaps = 37/364 (10%) Query: 5 EFVPSITIALVGSLAAGFLFAQVWMMITRRITEAP-SAIITQFGGTFMVWIIAEHLGLSG 63 EF+ L+ +A +L+++ +++R + P + I+ F +++AEHLG+SG Sbjct: 184 EFLKVAIGGLLAGVAITWLYSKSLRIMSRWSGDDPATQIVLLLLLPFASYLVAEHLGVSG 243 Query: 64 ILTIIAYAITISRTAPARTSA-RLRVSSYAVWETVVFVLNVLAFMLIGLQLRPI------ 116 IL +A +TIS++ R + +R+ + +VW + FV N + F+L+GLQL I Sbjct: 244 ILAAVAAGMTISQSGIIRNAPLAMRLRADSVWSMLEFVFNGMVFILLGLQLPGILETSIT 303 Query: 117 WSGLDDEVR-WKYCSFAAAILAVVILARILWVMPYGALLRTLKARNLLPAHVVDAVPTLK 175 + LD ++ W + A I ++L R W+ + + + + P D T + Sbjct: 304 QAELDPTIQTWNLFADVAIIYGALLLLRFGWLWSMKKISKRVLTKR--PLQFSDY--TTR 359 Query: 176 RGFIVSWCGMRGIVTLAAAFALPEW------FPYRDLILLTAFAVVLGSLVIQGLTLRPL 229 ++ S+ G+RG +TLA ++P + FP R ++ A V+L S++I + L PL Sbjct: 360 ELWVASFAGVRGAITLAGVLSIPLFLSDGSAFPSRYQLVFIATGVILLSVIIGVIALPPL 419 Query: 230 ILALNFADDDPVGREAARARGVVFRAALDEIDA------DPSEE---AEILR-LEYRAV- 278 + + AD E AR + A+ ++ SEE +E+L+ + R + Sbjct: 420 LRGVVVADKSASREEIRLARAAMAEVAIVSLNKMEERLMTSSEENIDSELLKEVSSRVIG 479 Query: 279 -LLRAENDPDGGLTSRELPSDPLRRR----AISAARQALLNLRRTEAIGDDAFHLVEEEL 333 L R D L + L RR A+ A R L +LR T+ I ++ + +L Sbjct: 480 TLRRRTGSKDE--VENTLLIENLERRFRLTALRAERGELYHLRATQKISNETLQKLLHDL 537 Query: 334 DRAE 337 D E Sbjct: 538 DLLE 541