BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0006.p (242 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll7725 [NU] COG3121 P pilus assembly protein, chaperone PapD Ml... 261 1e-69 AGl297 [NU] COG3121 P pilus assembly protein, chaperone PapD Atu... 190 2e-48 SMa1597 [NU] COG3121 P pilus assembly protein, chaperone PapD Sm... 171 2e-42 RSp1499 [NU] COG3121 P pilus assembly protein, chaperone PapD Rs... 124 1e-28 PA4651 [NU] COG3121 P pilus assembly protein, chaperone PapD Pae... 89 8e-18 >mll7725 [NU] COG3121 P pilus assembly protein, chaperone PapD Mlo Mesorhizobium loti Length = 272 Score = 261 bits (666), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 130/213 (61%), Positives = 161/213 (75%), Gaps = 3/213 (1%) Query: 27 RVAPTTLDLLMPDSAATLNLRNEDSRPINVQIRVFRWTQSGGADRLEPTTDVVASPPSQT 86 RVAPT ++++ PDSAA L LRN+ +PINVQIRVFRWTQ GG +RLEPT+DVVASPP+ Sbjct: 61 RVAPTNIEMIAPDSAAALTLRNDAKQPINVQIRVFRWTQEGGIERLEPTSDVVASPPAAK 120 Query: 87 LGPNADYVVRVVRVSKEPVVGEESYRVVVDELPDPARRRPGIVNLVLRYSVPVFFRGPDA 146 LGP ADY+VRVVRVSK V GEESYRV++DELPDP+R++ G V+LVLRYSVPVFFR PDA Sbjct: 121 LGPGADYLVRVVRVSKSAVAGEESYRVIIDELPDPSRKKAGTVSLVLRYSVPVFFRNPDA 180 Query: 147 RPGQVSWTVGRSGNALAVTAKNAGQQRLKIADLKLIDGAGKSVTLGKGLVGYVLGNATMQ 206 VSW++ G L +TAKN G+ RL+++DL L +G+ + GLVGYVLG ATMQ Sbjct: 181 AAPDVSWSLSGKGRGLLLTAKNTGESRLRLSDLTLTQ-SGRKLGNRNGLVGYVLGGATMQ 239 Query: 207 WSFPAHGTSLSSHAVTLSAQSDAGQINATAAVQ 239 W P G +LS AV LSAQSD G +A +++ Sbjct: 240 W--PIAGKTLSGGAVLLSAQSDFGPFDANVSIK 270 >AGl297 [NU] COG3121 P pilus assembly protein, chaperone PapD Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 238 Score = 190 bits (483), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 103/229 (44%), Positives = 141/229 (61%), Gaps = 1/229 (0%) Query: 13 FACLFATRVEAASLRVAPTTLDLLMPDSAATLNLRNEDSRPINVQIRVFRWTQSGGADRL 72 FA L + +AASLRVAP LDL P +A++L + N+ PINVQ+R+FRWTQ G D Sbjct: 11 FAGLSVSPSQAASLRVAPVLLDLKAPTAASSLRIWNDAKTPINVQVRIFRWTQQNGQDVY 70 Query: 73 EPTTDVVASPPSQTLGPNADYVVRVVRVSKEPVVGEESYRVVVDELPDPARRRPGIVNLV 132 DVVASPP L A+ ++R+VR+SK PV EESYR++VDELP + + G VNLV Sbjct: 71 TAVNDVVASPPMTQLKGGAENLIRIVRLSKTPVRAEESYRIIVDELPPAGKPQSGTVNLV 130 Query: 133 LRYSVPVFFRGPDARPGQVSWTVGRSGNALAVTAKNAGQQRLKIADLKLIDGAGKSVTLG 192 +R+S+PVFF + W V G V+ NAG++R++IA+L L G G+ + Sbjct: 131 VRHSIPVFFSPASTGDAEPVWKVTPQGKGYKVSVSNAGERRIRIANLILSGGNGQPIGRQ 190 Query: 193 KGLVGYVLGNATMQWSFPAHGTSLSSHAVTLSAQSDAGQINATAAVQAG 241 +GLVGYVLG + + PA G + + +SA S+ G +NATA V G Sbjct: 191 QGLVGYVLGKSAATFLVPATGGK-AGGTLKISADSEGGPVNATARVSGG 238 >SMa1597 [NU] COG3121 P pilus assembly protein, chaperone PapD Sme Sinorhizobium meliloti Length = 260 Score = 171 bits (432), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 97/232 (41%), Positives = 137/232 (59%), Gaps = 3/232 (1%) Query: 1 MPALLARVSGIAFACLFATRVEAASLRVAPTTLDLLMPDSAATLNLRNEDSRPINVQIRV 60 M ++L +A L+ A SL ++P++++ + PD AA +LRN+ RPI++++RV Sbjct: 23 MTSILKSTGAVALLLLYTLTANATSLMISPSSIERVAPDRAAVFHLRNQMDRPISIKVRV 82 Query: 61 FRWTQSGGADRLEPTTDVVASPPSQTLGPNADYVVRVVRVSKEPVVGEESYRVVVDELPD 120 FRW+Q GG ++LEPT DVVASP S L PN + VRVVRVSKEP+ EE YRVV+DE D Sbjct: 83 FRWSQKGGVEKLEPTGDVVASPISAQLSPNGNRAVRVVRVSKEPLRSEEGYRVVIDE-AD 141 Query: 121 PARRRPGIVNLVLRYSVPVFFRGPDARPGQVSWTVGRSGNALAVTAKNAGQQRLKIADLK 180 P R P +L R+ +PV FR PD ++ ++ RS L + +N G RL+++D+ Sbjct: 142 PTRNTPEAESLSARHVLPVLFRPPDVLGPEIELSLTRSDGWLMLVVENKGASRLRLSDVT 201 Query: 181 LIDGAGKSVTLGKGLVGYVLGNATMQWSFPAHGTSLSSHAVTLSAQSDAGQI 232 L G+ + +G VGYVL T W S S VT+SA S G I Sbjct: 202 LAQGSA-VIARREGFVGYVLPGLTRHWRV-GREDSYSGGIVTVSANSSGGAI 251 >RSp1499 [NU] COG3121 P pilus assembly protein, chaperone PapD Rso Ralstonia solanacearum Length = 241 Score = 124 bits (312), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 72/184 (39%), Positives = 102/184 (55%), Gaps = 6/184 (3%) Query: 27 RVAPTTLDLLMPDSAATLNLRNEDSRPINVQIRVFRWTQSGGADRLEPTTDVVASPPSQT 86 +++P LDLL SA +L LRN ++P+N Q+RVFRWTQ DRLEP +VASPP Sbjct: 37 QISPIRLDLLPGQSAGSLTLRNRGTQPLNAQVRVFRWTQDAEGDRLEPAPALVASPPIVQ 96 Query: 87 LGPNADYVVRVVRVSKEPVVGEESYRVVVDELPDPARRRPGI---VNLVLRYSVPVFFRG 143 + + +VRVV E++YR+++DELP+ RRP V + LRYSVPVF Sbjct: 97 ISGEGEQLVRVVLTQPAAGNDEQTYRLLIDELPE---RRPQAQTGVTMQLRYSVPVFVNA 153 Query: 144 PDARPGQVSWTVGRSGNALAVTAKNAGQQRLKIADLKLIDGAGKSVTLGKGLVGYVLGNA 203 P ++S + R G + +N + ++AD+ + G L GL+GY LG A Sbjct: 154 STGAPPRLSAALRRDGGRTLLVVRNPEARHAQLADVSIEWSDGTRSPLTAGLLGYALGGA 213 Query: 204 TMQW 207 T W Sbjct: 214 TRAW 217 >PA4651 [NU] COG3121 P pilus assembly protein, chaperone PapD Pae Pseudomonas aeruginosa Length = 262 Score = 89.4 bits (220), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 57/190 (30%), Positives = 95/190 (50%), Gaps = 21/190 (11%) Query: 41 AATLNLRNEDSRPINVQIRVFRWTQSGGADRLEPTTDVVASPPSQTLGPNADYVVRVVRV 100 A L L N +P+ +Q+RV W+Q+ D V+ SPP + P +VR++R Sbjct: 47 ATALWLENRGKQPVTLQVRVLGWSQADFQDVYRNQQAVIPSPPFVKVEPGRRQLVRLIRQ 106 Query: 101 SKEPVVGEESYRVVVDELPD----PARRRPGI-VNLVLRYSVPVFF-------------- 141 +P E++YRV++DE+PD +R PG+ + +RYSVP+F Sbjct: 107 GGQPSTPEDAYRVLIDEVPDGDAGQTQRSPGLALQFQMRYSVPLFVSADGVWTQPRSDVE 166 Query: 142 RGP-DARPGQVSWTVGRSGNALAVTAKNAGQQRLKIADLKLIDGAGKSVTLGKGLVGYVL 200 R P DA +++W + + +N G +++ ++ +G G+S+ L GL+GYVL Sbjct: 167 RDPADATRPKLAWRLVEEQGKRYLQVRNEGSVHARLSHVRW-EGNGRSLALLDGLLGYVL 225 Query: 201 GNATMQWSFP 210 M+W P Sbjct: 226 PGRQMRWPLP 235