BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0002.p (386 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll9608 [U] COG3451 Type IV secretory pathway, VirB4 components ... 463 e-130 mlr6400 [U] COG3451 Type IV secretory pathway, VirB4 components ... 352 8e-97 RSc2581 [U] COG3451 Type IV secretory pathway, VirB4 components ... 334 3e-91 AGpT83 [U] COG3451 Type IV secretory pathway, VirB4 components A... 246 9e-65 RC0141 [U] COG3451 Type IV secretory pathway, VirB4 components R... 145 2e-34 >mll9608 [U] COG3451 Type IV secretory pathway, VirB4 components Mlo Mesorhizobium loti Length = 816 Score = 463 bits (1192), Expect = e-130, Method: Compositional matrix adjust. Identities = 225/293 (76%), Positives = 250/293 (85%) Query: 1 MIPLSAVWAGPDRDEHFRSSPLFFGKTEGSTPFRFSLHVGDVGHTLIVGPTGAGKSVLLA 60 MIPLSAVWAGP+RDEH PL +GKTEGSTPFR LHVGDVGHTL+VGPTGAGKSVLLA Sbjct: 400 MIPLSAVWAGPERDEHLVGPPLLYGKTEGSTPFRLVLHVGDVGHTLVVGPTGAGKSVLLA 459 Query: 61 LMAIQFRRYRKSQIFAFDFGGSIRTAALAMGGDWHDLGGSLSNSAEDSVSLQPLARIGDA 120 LMA+QF RY +QIFAFDFGGSIR AA +MGGDWHDLGG L+ + SVSLQPLARI D Sbjct: 460 LMALQFCRYHNAQIFAFDFGGSIRAAAFSMGGDWHDLGGHLTEGMDASVSLQPLARIHDT 519 Query: 121 AERAWAAEWVTAILAKEGVTIDPTVKEHVWSALTSLASSPAEERTITGLTVLLQSTALKQ 180 ERAWAA+W+ A+L +EG+ I P KEH+W+ALTSLAS+P EERT+TGL+VLLQ+ LKQ Sbjct: 520 YERAWAADWIVALLMREGIQITPDAKEHIWAALTSLASAPLEERTLTGLSVLLQANDLKQ 579 Query: 181 ALQPYCVGGSSGRLLDAEAEQLGFASVQAFETEGLIGAGAAPAVLTYLFHRIEGRLDGRP 240 AL+PYC+GG GRLLDAEAE LG ASVQAFE EGL+GA AAPAVL+YLFHR+ RLDGRP Sbjct: 580 ALRPYCLGGPHGRLLDAEAEHLGQASVQAFEIEGLVGAQAAPAVLSYLFHRVSDRLDGRP 639 Query: 241 TLLIIDEGWLVLDDPAFAQQLREWLKTLRKKNASVVFATQSLSDIDGSNIAPA 293 TLLIIDEGWL LDD FA QLREWLKTLRKKNASVVFATQSL+DID S IAPA Sbjct: 640 TLLIIDEGWLALDDEGFAGQLREWLKTLRKKNASVVFATQSLADIDNSAIAPA 692 >mlr6400 [U] COG3451 Type IV secretory pathway, VirB4 components Mlo Mesorhizobium loti Length = 816 Score = 352 bits (903), Expect = 8e-97, Method: Compositional matrix adjust. Identities = 177/300 (59%), Positives = 221/300 (73%), Gaps = 13/300 (4%) Query: 1 MIPLSAVWAGPDRDEHF------RSSPLFFGKTEGSTPFRFSLHVGDVGHTLIVGPTGAG 54 ++PLS+VWAGP +EH + PL +T GSTPFR S HV DVGH LIVGPTGAG Sbjct: 401 LMPLSSVWAGPAANEHLAEVTGREAPPLLIAETSGSTPFRLSSHVEDVGHMLIVGPTGAG 460 Query: 55 KSVLLALMAIQFRRYRKSQIFAFDFGGSIRTAALAMGGDWHDLGGSLSNSAEDSVSLQPL 114 KSVLLAL+A+QFRRY +Q++ FD G S R A LAMGG+ H LG ++ +++ QPL Sbjct: 461 KSVLLALIALQFRRYAGAQVYVFDKGNSARAATLAMGGEHHVLG------SDGALAFQPL 514 Query: 115 ARIGDAAERAWAAEWVTAILAKEGVTIDPTVKEHVWSALTSLASSPAEERTITGLTVLLQ 174 IGD R+WAAEW+ ++A E VT+ P VKE +WSAL SLA++PA+ERT+TGL+VLLQ Sbjct: 515 RNIGDQGRRSWAAEWIAGLIAHENVTLTPEVKEAIWSALNSLATAPAQERTLTGLSVLLQ 574 Query: 175 STALKQALQPYCVGGSSGRLLDAEAEQLGFASVQAFETEGLIGA-GAAPAVLTYLFHRIE 233 S ALK AL PY + G GRLLDA+ ++L + VQ FETE L+ + A VLTYLFHR+E Sbjct: 575 SNALKSALMPYTLDGPFGRLLDADDDRLALSDVQCFETEELMHSQSAVLPVLTYLFHRLE 634 Query: 234 GRLDGRPTLLIIDEGWLVLDDPAFAQQLREWLKTLRKKNASVVFATQSLSDIDGSNIAPA 293 R DGRPTLL++DE W+ LD+P FA ++REWLK LRKKN SV+FATQSL+DI GS IAPA Sbjct: 635 ERFDGRPTLLMLDEAWVYLDNPLFAARIREWLKVLRKKNVSVIFATQSLADIAGSAIAPA 694 >RSc2581 [U] COG3451 Type IV secretory pathway, VirB4 components Rso Ralstonia solanacearum Length = 816 Score = 334 bits (856), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 167/294 (56%), Positives = 212/294 (72%), Gaps = 7/294 (2%) Query: 1 MIPLSAVWAGPDRDEHFRSSPLFFGKTEGSTPFRFSLHVGDVGHTLIVGPTGAGKSVLLA 60 M+PLSAVWAGP+R++H P+ +T+G+TPFR H+GDVGH +VGPTG GKS LA Sbjct: 400 MMPLSAVWAGPERNKHLDGPPVIVTRTDGATPFRLVTHIGDVGHMAVVGPTGMGKSAFLA 459 Query: 61 LMAIQFRRYRKSQIFAFDFGGSIRTAALAMGGDWHDLGGSLSNSAEDSVSLQPLARIGDA 120 ++A+QFRRY S+IF FD G S+R L +GG+ +DLG A+ ++ QPLA I Sbjct: 460 VLAMQFRRYLHSRIFVFDMGRSMRATILGLGGEHYDLG------ADGGIAFQPLAHIDAE 513 Query: 121 AERAWAAEWVTAILAKEGVTIDPTVKEHVWSALTSLASSPAEERTITGLTVLLQSTALKQ 180 R WAAEWV L EGVT+ P K +WSAL SLA +P E+RT+TGL+VLLQS AL+Q Sbjct: 514 GYRTWAAEWVEGRLLHEGVTVGPDEKAGIWSALGSLAGAPVEQRTMTGLSVLLQSNALRQ 573 Query: 181 ALQPYCVGGSSGRLLDAEAEQLGFASVQAFETEGLIGAGAAP-AVLTYLFHRIEGRLDGR 239 AL PY +GG+ G+LLDA+ ++LG A VQ FE E L+ + AA +VL YLF R + R DG Sbjct: 574 ALAPYVLGGAHGKLLDADRDRLGSADVQCFEMEELMASPAAVRSVLGYLFARFDERFDGS 633 Query: 240 PTLLIIDEGWLVLDDPAFAQQLREWLKTLRKKNASVVFATQSLSDIDGSNIAPA 293 PTLLI+DE WL LDDP FA ++R+WLKTLRK+N SVVFATQSL+DI S+IA A Sbjct: 634 PTLLILDESWLFLDDPVFAARIRQWLKTLRKRNVSVVFATQSLADIQNSSIAAA 687 >AGpT83 [U] COG3451 Type IV secretory pathway, VirB4 components Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 822 Score = 246 bits (627), Expect = 9e-65, Method: Compositional matrix adjust. Identities = 141/329 (42%), Positives = 190/329 (57%), Gaps = 19/329 (5%) Query: 1 MIPLSAVWAG-PDRDEHFR--SSPLFFGKTEGSTPFRFSLHVGDVGHTLIVGPTGAGKSV 57 +IPL++VW+G P F SP GSTPFR SLHV DVGHTL+ GPTG+GKS Sbjct: 412 LIPLNSVWSGNPVAPCPFYPAGSPPLMQVASGSTPFRLSLHVDDVGHTLVFGPTGSGKST 471 Query: 58 LLALMAIQFRRYRKSQIFAFDFGGSIRTAALAMGGDWHDLGGSLSNSAEDS---VSLQPL 114 LLAL+A QFRRY +QIFAFD G S+ LA+GGD +++ G + E + ++ PL Sbjct: 472 LLALIAAQFRRYAGAQIFAFDKGRSMLPLTLAIGGDHYEIDGDAAGEGEGASPRLAFCPL 531 Query: 115 ARIGDAAERAWAAEWVTAILAKEGVTIDPTVKEHVWSALTSLASSPAEERTITGLTVLLQ 174 A + +RAWAAEW+ ++A +GVT+ P + + L +A S R+++ +Q Sbjct: 532 AELSTDGDRAWAAEWIETLVALQGVTVTPDYRNAISRQLVLMAESRG--RSLSDFVSGVQ 589 Query: 175 STALKQALQPYCVGGSSGRLLDAEAEQLGFASVQAFETEGLIGAGAAP--AVLTYLFHRI 232 +K AL Y V G G+LLDAE + L + Q FE E L+ G VL Y+F R+ Sbjct: 590 MREIKDALHHYTVDGPMGQLLDAEEDGLALGAFQCFEVEELMNMGERNLVPVLLYMFRRV 649 Query: 233 EGRLDGRPTLLIIDEGWLVLDDPAFAQQLREWLKTLRKKNASVVFATQSLSDIDGSNI-- 290 E RL G P+L+IIDE WL+L P F ++REWLK LRK N +VV ATQS+SD + S I Sbjct: 650 EKRLTGAPSLIIIDEAWLMLGHPTFRDKIREWLKVLRKANCAVVLATQSISDAERSGIID 709 Query: 291 -----APAXTA--QGMVEDPAEEERFRRL 312 P G +P E + R+ Sbjct: 710 VLKESCPTKICLPNGAAREPGTREFYERI 738 >RC0141 [U] COG3451 Type IV secretory pathway, VirB4 components Rco Rickettsia conorii Length = 805 Score = 145 bits (365), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 88/294 (29%), Positives = 145/294 (49%), Gaps = 14/294 (4%) Query: 10 GPDRDEHFRSSPLFFGKTEGSTPFRFSLHVGDVGHTLIVGPTGAGKSVLLALMAIQFRRY 69 G RD H+ T G TPF F+ HV DVGHTLI+GPTGAGK+VL+ + + +++ Sbjct: 420 GKIRDNHWGEYVTVLDTTSG-TPFYFNFHVRDVGHTLIIGPTGAGKTVLMNFLCAEAQKF 478 Query: 70 RKSQIFAFDFGGSIRTAALAMGGDWHDLGGSLSNSAEDSVSLQPLARIGDAAERAWAAEW 129 K ++F FD A+ G + + L + PL + R + EW Sbjct: 479 -KPRMFFFDKDRGAEIFIRALNGVYTVIDPGLK------CNFNPLQLEDTSENRTFILEW 531 Query: 130 VTAILAKEGVTIDPTVKEHVWSALTSLASSPAEERTITGLTVLL---QSTALKQALQPYC 186 + ++ G ++ + + A++ ++R ++ + L +L + + Sbjct: 532 LRVLVTSNGESLTAQDNKILSQAVSGNFRLAKQDRRLSNVIAFLGIDTPNSLASRIAMWV 591 Query: 187 VGGSSGRLLDAEAEQLGFASVQAF---ETEGLIGAGAAPAVLTYLFHRIEGRLDGRPTLL 243 GS ++ D E + + + F TE L + VL Y+FHRI LDG+ T++ Sbjct: 592 GKGSHAKIFDNEIDDIDLQKARVFGFDMTELLKDPVSLAPVLLYIFHRINISLDGQKTMI 651 Query: 244 IIDEGWLVLDDPAFAQQLREWLKTLRKKNASVVFATQSLSDIDGSNIAPAXTAQ 297 ++DE W ++D+P FA ++++WLK LRK N V+FATQS+ D S I+ Q Sbjct: 652 VLDEAWALIDNPVFAPKIKDWLKVLRKLNTFVIFATQSVEDAAKSRISDTLIQQ 705