BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0001.p (277 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value aq_1132 [M] COG0845 Membrane-fusion protein Aae Aquifex aeolicus 134 2e-31 RSp0927 [M] COG0845 Membrane-fusion protein Rso Ralstonia solana... 132 1e-30 ylcD [M] COG0845 Membrane-fusion protein Eco Escherichia coli K12 112 9e-25 ECs0612 [M] COG0845 Membrane-fusion protein Ecs Escherichia coli... 112 1e-24 ZylcD [M] COG0845 Membrane-fusion protein EcZ Escherichia coli O... 112 1e-24 >aq_1132 [M] COG0845 Membrane-fusion protein Aae Aquifex aeolicus Length = 444 Score = 134 bits (338), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 86/254 (33%), Positives = 127/254 (50%), Gaps = 24/254 (9%) Query: 1 VTKGQPLMEIYSPAISSAAAEYVATITSKATVGIEPYGRGSKQRLTNLDVPEPVIAE-ME 59 V KG PLM++ SP I A EY +L E +I E + Sbjct: 201 VKKGTPLMKVLSPDIEIAKREY---------------------KLAKKIGDEKLIKEALR 239 Query: 60 KSRTVPIAIQWLSPRDGIVLQRAAIEGMRAQPGDVLFRIADVSVVWALVDVAERDLGNIV 119 K R + SP DG++LQ+ EG + G +RIAD+S W + +V I Sbjct: 240 KLRYLKAGSVVRSPVDGVILQKQVYEGGYLKEGQTAYRIADLSTAWIVAEVPLHFSSYIR 299 Query: 120 VGQKVTVRARSYPGRSFAGQISVIYPQVNKETRTARVRIELQNADLALLPDMYVDAEIDT 179 G K V F + I+P+VNK +RT +VR++L+N +L P+ V+ + Sbjct: 300 KGLKAFVSPEG-DSTQFQATVDYIFPEVNKLSRTLKVRLKLKNENLTFKPNALVNVSFEV 358 Query: 180 GGAAPVLTVADNSILDTGSRQSVLIDRGNGRFEPREVKLGRRGEGYVEIRDGLAEGDAVV 239 V+ V +++++DTG R+ V ++ G + P +VKLGRRGEGY E+ GL EG VV Sbjct: 359 P-IGEVIAVPESAVVDTGRRRFVFVEMEKGMYMPVQVKLGRRGEGYYEVIHGLKEGQRVV 417 Query: 240 TSATFLIDAESNLK 253 TFL+DAE+ +K Sbjct: 418 VKGTFLLDAEAQIK 431 >RSp0927 [M] COG0845 Membrane-fusion protein Rso Ralstonia solanacearum Length = 513 Score = 132 bits (331), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 86/255 (33%), Positives = 131/255 (51%), Gaps = 6/255 (2%) Query: 1 VTKGQPLMEIYSPAISSAAAEYVATITSKATVGIEPYGRGSKQRLTNLDVPEPVIAEMEK 60 V +GQ L+ +YSP +A EY+A ++ +A G +K R+ + I+ + Sbjct: 164 VRRGQTLVSLYSPDWVAAQEEYLA-VSRQAAHGQSDLVGAAKARMLQAGMTPGQISAVTA 222 Query: 61 SRTVPIAIQWLSPRDGIVLQRAAIEGMRAQPGDVLFRIADVSVVWALVDVAERDLGNIVV 120 S + + SP DG V++ A +GM PG LFR+A++S VW + +V E + Sbjct: 223 SGKLQPGLAITSPIDGFVMEVAVRDGMTVTPGMTLFRLANLSRVWIIAEVPEAQRRTVAP 282 Query: 121 GQ--KVTVRARSYPGRSFAGQISVIYPQVNKETRTARVRIELQNADLALLPDMYVDAEID 178 G KV+V + P + + I P VN TRT +VRI L N D L+P M+ D Sbjct: 283 GAPVKVSVAGLAEPETA---TVDAILPDVNAATRTIKVRIVLPNQDKRLVPGMFATVRFD 339 Query: 179 TGGAAPVLTVADNSILDTGSRQSVLIDRGNGRFEPREVKLGRRGEGYVEIRDGLAEGDAV 238 G L + +++ TG V++D G F P E++ GR +G VE+ GL G V Sbjct: 340 GGQDKEALLIPSEAVIRTGQHSIVMVDGGQAGFVPTEIQTGREADGRVEVLQGLQAGQKV 399 Query: 239 VTSATFLIDAESNLK 253 VTS FLID+E++L+ Sbjct: 400 VTSGQFLIDSEASLR 414 >ylcD [M] COG0845 Membrane-fusion protein Eco Escherichia coli K12 Length = 407 Score = 112 bits (280), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 76/267 (28%), Positives = 132/267 (49%), Gaps = 4/267 (1%) Query: 1 VTKGQPLMEIYSPAISSAAAEYVATITSKATVGIEPYGRGSKQRLTNLDVPEPVIAEMEK 60 V KG PL+++ P A +EY+ + T G G +RL +PE I + Sbjct: 144 VQKGTPLLDLTIPDWVEAQSEYLLL---RETGGTATQTEGILERLRLAGMPEADIRRLIA 200 Query: 61 SRTVPIAIQWLSPRDGIVLQRAAIEGMRAQPGDVLFRIADVSVVWALVDVAERDLGNIVV 120 ++ + +P DG++ GM +V+ +I + VW + E + Sbjct: 201 TQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKD 260 Query: 121 GQKVTVRARSYPGRSFAGQISVIYPQVNKETRTARVRIELQNADLALLPDMYVDAEIDTG 180 + T+ + P ++ + + P V+ TRT ++R+E+ NAD AL P M +++T Sbjct: 261 ASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTA 320 Query: 181 GAAPVLTVADNSILDTGSRQSVLIDRGNGRFEPREVKLGRRGEGYVEIRDGLAEGDAVVT 240 + P+L + +++DTGS Q V+ +GRF P+ V + + +G +R GLAEG+ VV+ Sbjct: 321 -SEPMLLIPSQALIDTGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVS 379 Query: 241 SATFLIDAESNLKAAIKGFAEAGAAQA 267 S FLID+E+N+ A++ A A Sbjct: 380 SGLFLIDSEANISGALERMRSESATHA 406 >ECs0612 [M] COG0845 Membrane-fusion protein Ecs Escherichia coli O157:H7 Length = 407 Score = 112 bits (280), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 76/267 (28%), Positives = 132/267 (49%), Gaps = 4/267 (1%) Query: 1 VTKGQPLMEIYSPAISSAAAEYVATITSKATVGIEPYGRGSKQRLTNLDVPEPVIAEMEK 60 V KG PL+++ P A +EY+ + T G G +RL +PE I + Sbjct: 144 VQKGAPLLDLTIPDWVEAQSEYLLL---RETGGTATQTEGILERLRLAGMPEADIRRLIA 200 Query: 61 SRTVPIAIQWLSPRDGIVLQRAAIEGMRAQPGDVLFRIADVSVVWALVDVAERDLGNIVV 120 ++ + +P DG++ GM +V+ +I + VW + E + Sbjct: 201 TQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKD 260 Query: 121 GQKVTVRARSYPGRSFAGQISVIYPQVNKETRTARVRIELQNADLALLPDMYVDAEIDTG 180 + T+ + P ++ + + P V+ TRT ++R+E+ NAD AL P M +++T Sbjct: 261 ASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTA 320 Query: 181 GAAPVLTVADNSILDTGSRQSVLIDRGNGRFEPREVKLGRRGEGYVEIRDGLAEGDAVVT 240 + P+L + +++DTGS Q V+ +GRF P+ V + + +G +R GLAEG+ VV+ Sbjct: 321 -SEPMLLIPSQALIDTGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVS 379 Query: 241 SATFLIDAESNLKAAIKGFAEAGAAQA 267 S FLID+E+N+ A++ A A Sbjct: 380 SGLFLIDSEANISGALERMRSESATHA 406 >ZylcD [M] COG0845 Membrane-fusion protein EcZ Escherichia coli O157:H7 EDL933 Length = 407 Score = 112 bits (279), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 76/267 (28%), Positives = 132/267 (49%), Gaps = 4/267 (1%) Query: 1 VTKGQPLMEIYSPAISSAAAEYVATITSKATVGIEPYGRGSKQRLTNLDVPEPVIAEMEK 60 V KG PL+++ P A +EY+ + T G G +RL +PE I + Sbjct: 144 VQKGAPLLDLTIPDWVEAQSEYLLL---RETGGTATQTEGILERLRLAGMPEADIRRLIA 200 Query: 61 SRTVPIAIQWLSPRDGIVLQRAAIEGMRAQPGDVLFRIADVSVVWALVDVAERDLGNIVV 120 ++ + +P DG++ GM +V+ +I + VW + E + Sbjct: 201 TQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKD 260 Query: 121 GQKVTVRARSYPGRSFAGQISVIYPQVNKETRTARVRIELQNADLALLPDMYVDAEIDTG 180 + T+ + P ++ + + P V+ TRT ++R+E+ NAD AL P M +++T Sbjct: 261 ASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLKLNTA 320 Query: 181 GAAPVLTVADNSILDTGSRQSVLIDRGNGRFEPREVKLGRRGEGYVEIRDGLAEGDAVVT 240 + P+L + +++DTGS Q V+ +GRF P+ V + + +G +R GLAEG+ VV+ Sbjct: 321 -SEPMLLIPSQALIDTGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVS 379 Query: 241 SATFLIDAESNLKAAIKGFAEAGAAQA 267 S FLID+E+N+ A++ A A Sbjct: 380 SGLFLIDSEANISGALERMRSESATHA 406