BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0135.p (420 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEII0128 [S] COG5441 Uncharacterized conserved protein Bme Bruc... 531 e-150 YPO3839 [S] COG5441 Uncharacterized conserved protein Ype Yersin... 160 4e-39 SMa1334 [S] COG5441 Uncharacterized conserved protein Sme Sinorh... 158 2e-38 mlr3387 Non COG 138 2e-32 mll9388 Non COG 137 6e-32 >BMEII0128 [S] COG5441 Uncharacterized conserved protein Bme Brucella melitensis Length = 418 Score = 531 bits (1368), Expect = e-150, Method: Compositional matrix adjust. Identities = 249/411 (60%), Positives = 318/411 (77%) Query: 5 TQPRILLIGTGDTKADELLFMSECIARAGGRTIMMDISVLGDPPYQAAHDKHAVAAAAQT 64 PRI++IGTGDTK+DELLFM++ I RAGG +M+D+S+LG+PPY+ A+ KH VA AA T Sbjct: 6 NSPRIMVIGTGDTKSDELLFMADVIERAGGSPVMIDVSILGNPPYEPAYSKHDVAEAAGT 65 Query: 65 TIEAIASSGDENSAMSLMAVGAANLAHALAERDAVDGVIAIGGTMGTDLALDVAQALPLG 124 T++AI SGDE+SAM+LMA GA L L++R VDG+IA+GG++GTDLALD+A LPL Sbjct: 66 TVQAIIDSGDEHSAMALMAEGATALVRGLSQRGQVDGMIALGGSLGTDLALDIAAILPLV 125 Query: 125 LPKLIVSTVAFSHLIPPVRVAADMMMILWAGGLYGLNGTCTAVLSQACGAIVGAARSAIK 184 +PK IVST+A+SHL+PP R+A D+MMILWAGGLYGLN C +VLSQACGA+VGAA+ K Sbjct: 126 VPKFIVSTIAYSHLLPPERIAPDLMMILWAGGLYGLNPICRSVLSQACGAVVGAAKLVEK 185 Query: 185 PRKDRPVVAITSLGKSCLRYMVELKPQLERRGYEVVVFHTTGMGGRAMESIAAQGGFAAV 244 P ++P++ +T LG SCL+YM LKP+LE+RGY+V +FH TGMGGRA E++AAQ GF AV Sbjct: 186 PSAEKPLIGMTHLGSSCLKYMRFLKPELEKRGYDVAIFHATGMGGRAYEAVAAQKGFVAV 245 Query: 245 LDLSLQEVANQLTGSVVNSGADRLENAGRAGIPQIVAPGAIDMVDFPAWLGVPNELSDRP 304 D +QEV N +GSVV SG DR+ENAGRAGIPQI+APGA+DMVD PAW VP + DRP Sbjct: 246 FDFCIQEVTNAESGSVVTSGPDRMENAGRAGIPQIIAPGAVDMVDMPAWQNVPEQFRDRP 305 Query: 305 FHAHNRLLASATSGSTDRQRIARAIAGKLSQASAPVAFVLPAGGIQQWDREGEALHDPDA 364 +HAHNRL+AS T R+ +AR +A KL +A+APVAF+LP GG+Q+WDR GE LH+P+A Sbjct: 306 YHAHNRLIASITVSPEQRRAVARVVAAKLERAAAPVAFILPTGGVQEWDRNGEPLHEPEA 365 Query: 365 LAAFVDAMRSAIKAPVELHEIKDHINSAAFNETVLGIFDRWVAAGIVPRGR 415 L AF+D MR A+ + E+ HIN+ F L +FDRWVA GIV +G Sbjct: 366 LGAFLDEMRGAVSGTITFEEVDAHINAPEFASRALAVFDRWVAEGIVVKGN 416 >YPO3839 [S] COG5441 Uncharacterized conserved protein Ype Yersinia pestis Length = 405 Score = 160 bits (406), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 118/376 (31%), Positives = 181/376 (48%), Gaps = 15/376 (3%) Query: 7 PRILLIGTG-DTKADELLFMSECIARAGGRTIMMDISVLGDPPYQAAHDKHAVAAAAQTT 65 PR + I T DTK EL ++SE I G T+ +D+S A A Sbjct: 2 PRHIYIATTTDTKGQELAYVSELIQATGLTTVTVDLSTKESQRDSGADICAETVAGYHPD 61 Query: 66 IEAIASSGDENSAMSLMAVGAANLAHALAERDAVDGVIAIGGTMGTDLALDVAQALPLGL 125 GD A++ MA+ + R V ++ +GG+ GT L Q LP+G+ Sbjct: 62 GRQAVFCGDRGQAINAMAIA---FERFMVSRVDVAALLGMGGSGGTALITPAMQRLPIGI 118 Query: 126 PKLIVSTVAFSHLIPPVRVAADMMMILWAGGLYGLNGTCTAVLSQACGAIVGAARSAIK- 184 PKL+VST+A S + A+D+ M+ + GLN VLS A + GA A + Sbjct: 119 PKLMVSTMA-SGDVSGYIGASDIAMMYSVTDIAGLNRISRRVLSNAAHQVAGAVYFAQEA 177 Query: 185 -PRKDRPVVAITSLGKS--CLRYM-VELKPQLERRGYEVVVFHTTGMGGRAMESIAAQGG 240 P D+P + +T G + C++ + EL + Y+ +VFH TG GG+AME +A G Sbjct: 178 SPVDDKPALGLTMFGVTTPCIQAVSAELSDE-----YDCLVFHATGSGGKAMEKLAESGL 232 Query: 241 FAAVLDLSLQEVANQLTGSVVNSGADRLENAGRAGIPQIVAPGAIDMVDFPAWLGVPNEL 300 A VLDL+ EV + L V+ G +R + IP + + GA+DMV+F + +P + Sbjct: 233 LAGVLDLTTTEVCDLLFDGVLACGPERFDAIAHTHIPYVGSCGALDMVNFGSPATIPAKY 292 Query: 301 SDRPFHAHNRLLASATSGSTDRQRIARAIAGKLSQASAPVAFVLPAGGIQQWDREGEALH 360 +DR F+ HN + + + +AR I KL++ V F++P GG D G+ Sbjct: 293 ADRLFYKHNAQVTLMRTTEQENIEMARWIGEKLNRCQGEVRFLIPEGGFSALDAPGQPFW 352 Query: 361 DPDALAAFVDAMRSAI 376 D AL AF+ + + Sbjct: 353 DEKALTAFIRTLEETV 368 >SMa1334 [S] COG5441 Uncharacterized conserved protein Sme Sinorhizobium meliloti Length = 398 Score = 158 bits (399), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 131/400 (32%), Positives = 202/400 (50%), Gaps = 15/400 (3%) Query: 8 RILLIGTGDTKADELLFMSECIARAGGRTIMMDISVLGDPPYQAAHDKHAVAAAAQTTIE 67 RI ++GT DTK +EL++++ C+ AGGR +++D+ P VAA Sbjct: 3 RIYVVGTADTKGEELVYLASCVEAAGGRPVLVDVGTR-RPTVLVDISAETVAAVHPGGAA 61 Query: 68 AIASSGDENSAMSLMAVGAANLAHALAERDAVDGVIAIGGTMGTDLALDVAQALPLGLPK 127 A+ S D +A++ A+G A A L RD V GV+ +GG GT + + LPLGLPK Sbjct: 62 AVLSGNDRGTAIA--AMGEA-FARFLPARDDVAGVVGMGGGGGTSIITAGMRRLPLGLPK 118 Query: 128 LIVSTVAFSHLIPPVRVAADMMMILWAGGLYGLNGTCTAVLSQACGAIVGAARSAIKPRK 187 ++VST+A + P V V+ D++M+ + GLN +L A AI A + Sbjct: 119 VMVSTLASGDVGPYVDVS-DIIMMPSVTDMAGLNRVSRVILKNAAEAITAMANRPAEETA 177 Query: 188 DRPVVAITSLGKS--CLRYMVE-LKPQLERRGYEVVVFHTTGMGGRAMESIAAQGGFAAV 244 +P + +T G + C+ +VE LK ++ +VFH TG GGRAME +A G + V Sbjct: 178 SKPAIGLTMFGVTTPCVTAIVERLKAD-----HDCLVFHATGTGGRAMEKLADSGLLSGV 232 Query: 245 LDLSLQEVANQLTGSVVNSGADRLENAGRAGIPQIVAPGAIDMVDFPAWLGVPNELSDRP 304 LD++ EV + + G V+ + DR R +P + + GA+DMV+F A VP S R Sbjct: 233 LDITTTEVCDLVFGGVLPATEDRFGAIARTDLPYVGSVGALDMVNFWAPETVPERYSGRL 292 Query: 305 FHAHNRLLASATSGSTDRQRIARAIAGKLSQASAPVAFVLPAGGIQQWDREGEALHDPDA 364 + HN + + + I R I KL+ S P+ F++P G+ D EG A DP A Sbjct: 293 LYRHNPNVTLMRTTPEECAAIGRWIGAKLNLCSGPLRFLIPERGVSALDIEGGAFFDPAA 352 Query: 365 LAAFVDAMRSAIKAP--VELHEIKDHINSAAFNETVLGIF 402 AA +A+ + + + + HIN F E + + Sbjct: 353 DAALFEALETTVNRSDRRRIERLPLHINDPQFAEAAVAAY 392 >mlr3387 Non COG Length = 397 Score = 138 bits (348), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 125/400 (31%), Positives = 194/400 (48%), Gaps = 13/400 (3%) Query: 8 RILLIGTGDTKADELLFMSECIARAGGRTIMMDISVLGDPPYQAAHDKHAVAAAAQTTIE 67 RI ++GT DTK +EL F+++ IA G +D+ D + +A + Sbjct: 3 RIYVVGTADTKGEELAFLADAIAATGAAVSRVDVGTR-DATIPVDISANDIAGHHPGGRD 61 Query: 68 AIASSGDENSAMSLMAVGAANLAHALAERDAVDGVIAIGGTMGTDLALDVAQALPLGLPK 127 A+ D +A++ M V A A + R+ + +I IGG GT + +ALPLGLPK Sbjct: 62 AVLGGNDRGAAVAAMGVAFARFAQS---RNDIAAMIGIGGGGGTSIVTSGMRALPLGLPK 118 Query: 128 LIVSTVAFSHLIPPVRVAADMMMILWAGGLYGLNGTCTAVLSQACGAIVGAARSAIKPRK 187 ++VST+A P V V+ D++M+ + GLN VL A AI G A P Sbjct: 119 IMVSTLASGDTAPYVDVS-DIIMMPSVTDMAGLNRLSRVVLHNAAQAIAGMAARPAPPPD 177 Query: 188 DRPVVAITSLGKS--CLRYMVELKPQLERRGYEVVVFHTTGMGGRAMESIAAQGGFAAVL 245 +P + +T G + C+ + + R Y+ +VFH TG GGR+ME +A G + V+ Sbjct: 178 GKPSIGLTMFGVTTPCVTTIADAL----RSTYDCMVFHATGTGGRSMEKLADSGLLSGVI 233 Query: 246 DLSLQEVANQLTGSVVNSGADRLENAGRAGIPQIVAPGAIDMVDFPAWLGVPNELSDRPF 305 D++ EV + L G V+ + DR R G+P + + GA+DMV+F A VP R F Sbjct: 234 DITTTEVCDLLLGGVLPATEDRFGAIARTGLPYVGSVGALDMVNFWAPSTVPERYRGRLF 293 Query: 306 HAHNRLLASATSGSTDRQRIARAIAGKLSQASAPVAFVLPAGGIQQWDREGEALHDPDAL 365 + HN + + + + + I I +L+ PV F++P G+ D EG D +A Sbjct: 294 YEHNPNVTLMRTSADECRAIGEWIGTRLALCDGPVHFLIPEKGVSALDIEGGTFFDREAD 353 Query: 366 AAFVDAMRSAIK--APVELHEIKDHINSAAFNETVLGIFD 403 A +A+ IK A + + HIN F + V D Sbjct: 354 AVLFEAIERTIKPNANRRVTRLPLHINDPEFAKAVAAFLD 393 >mll9388 Non COG Length = 408 Score = 137 bits (344), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 113/391 (28%), Positives = 187/391 (47%), Gaps = 12/391 (3%) Query: 9 ILLIGTGDTKADELLFMSECIARAGGRTIMMDISVLGDPPYQAAHDKHAVAAAAQTTIEA 68 + ++GT DTK EL ++ + I R G +++ + + +A+ A A+ Sbjct: 10 VYVVGTCDTKGSELRYLRDLI-RDAGCDVVLVDVSVSEFHSEASDVDVQPAEVARCHPNP 68 Query: 69 IASSGDENSAMSLMAVGAANLAHALAERDAVDGVIAIGGTMGTDLALDVAQALPLGLPKL 128 + + ++ ++ A+ A L + R VDG+I GG+ GT L +ALP+G+PK+ Sbjct: 69 LKAEELKDRGKAVAAMSQA-LVEYIRSRPDVDGIIGAGGSGGTALIAPAMRALPIGVPKV 127 Query: 129 IVSTVAFSHLIPPVRVAADMMMILWAGGLYGLNGTCTAVLSQACGAIVGAARSAIKPRKD 188 +VSTVA ++ P V D+ M+ + GLN VL+ A +I G + D Sbjct: 128 LVSTVASGNVAPYVG-PTDISMMYSVTDVSGLNRISRVVLANAAHSIAGMVLKQVGAAAD 186 Query: 189 -RPVVAITSLGKS--CLRYMVELKPQLERRGYEVVVFHTTGMGGRAMESIAAQGGFAAVL 245 RP + +T G + C++ + LE ++ +VFH TG GG++ E +A G + Sbjct: 187 ERPAIGLTMFGVTTPCVQAVTR---ALEA-NFDCLVFHATGTGGQSFEKLADSGLLVGGI 242 Query: 246 DLSLQEVANQLTGSVVNSGADRLENAGRAGIPQIVAPGAIDMVDFPAWLGVPNELSDRPF 305 D+S EV + L G V DR R +P + + GA+DMV+F A VP++ R Sbjct: 243 DVSTTEVCDYLVGGVFPCTPDRFGAFARTKLPYVGSCGALDMVNFGAMETVPSQFRSRRL 302 Query: 306 HAHNRLLASATSGSTDRQRIARAIAGKLSQASAPVAFVLPAGGIQQWDREGEALHDPDAL 365 H HN + + + RI I +L+ PV F++P G+ D G+ HDP+A Sbjct: 303 HVHNPQVTLMRTNPEECSRIGEWIGERLNLCEGPVRFLIPELGVSAIDAPGQPFHDPEAD 362 Query: 366 AAFVDAMRSAIKA--PVELHEIKDHINSAAF 394 A A+ ++ +L + HIN F Sbjct: 363 AVLFAALERTLRCTDKRQLARVPLHINDPQF 393