BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0116.p (467 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Rv3375 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 431 e-120 MT3485 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 431 e-120 PA4483 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 251 2e-66 BH0666 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 249 7e-66 BS_yerM [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 242 2e-63 >Rv3375 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Mtu Mycobacterium tuberculosis H37Rv Length = 475 Score = 431 bits (1107), Expect = e-120, Method: Compositional matrix adjust. Identities = 229/466 (49%), Positives = 307/466 (65%), Gaps = 2/466 (0%) Query: 1 MSADSLHYKSITEISELIRRKEAKPSEITEAMLGRIAKLDGQFHGYALVLAERAMAQAKQ 60 + D++ +TE+++LIR ++ +E+TE+ L RI +LD Q YA V+ E A+A A+ Sbjct: 12 LDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARA 71 Query: 61 YDAEIAKGIWRGPLHGVPIGLKDLCYTSFAPTAGGTTIHAKFVPSFNATIVDRLEVAGAV 120 DA+IA+G + G LHGVPIG+KDLCYT APTA GTTI F P+++AT+V RL AGAV Sbjct: 72 ADADIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAV 131 Query: 121 TLGKLKMTEGAYTSHHPAIQAPLNPWNVNYWVGSSSTGSGVATSAGLCYGAIGSDTGGSI 180 +GKL MTEGAY +HP++ P+NPW+ W G SS+G GVAT+AGLC+G+IGSDTGGSI Sbjct: 132 IIGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGSI 191 Query: 181 RFPSATCGLTGIKPTWGRVSRHGVFPLADSLDHVGPMCRSAADAAAMLGVIAGADINDPT 240 RFP++ CG+TGIKPTWGRVSRHGV LA S DHVGP+ RSA DAA +L VIAG+DI+DP+ Sbjct: 192 RFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSVIAGSDIHDPS 251 Query: 241 ALRAPVPNYLAGIDDGIRGLRIGVDRRYTQEGIDRHVVAALVQAERTLADLGADIREVHF 300 PVP+Y A + R R+GVD T D A L +TL D+G + +V Sbjct: 252 CSAEPVPDYAADL-ALTRIPRVGVDWSQTTS-FDEDTTAMLADVVKTLDDIGWPVIDVKL 309 Query: 301 PPYQKLVSQWIAMCSVETAEAHLDTYPSRKSEYGPDLAQLIEQGRATSGVDIAAIHHERL 360 P +V+ + M +VETA AH DTYP+R EYGP + +I+ G + V+ + RL Sbjct: 310 PALAPMVAAFGKMRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVEYQTLTERRL 369 Query: 361 KFSGSLAAMFEDIDLLLIPTMPVPIPTLTKMSEYGADPSVLLSILRFTAVFDFSGSPTIT 420 +F+ SL +F D+D+LL+P+ + PTL M G DP + + TA F+ SG+P I Sbjct: 370 EFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPFNVSGNPAIC 429 Query: 421 LPMGMASDQMPLSMQLVGPHLSEDVLVRAGAAFQSVTDWHTRRPPI 466 LP G + PL +Q +G E +LVRAG AFQ VT +H RRPP+ Sbjct: 430 LPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV 475 >MT3485 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases MtC Mycobacterium tuberculosis CDC1551 Length = 475 Score = 431 bits (1107), Expect = e-120, Method: Compositional matrix adjust. Identities = 229/466 (49%), Positives = 307/466 (65%), Gaps = 2/466 (0%) Query: 1 MSADSLHYKSITEISELIRRKEAKPSEITEAMLGRIAKLDGQFHGYALVLAERAMAQAKQ 60 + D++ +TE+++LIR ++ +E+TE+ L RI +LD Q YA V+ E A+A A+ Sbjct: 12 LDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARA 71 Query: 61 YDAEIAKGIWRGPLHGVPIGLKDLCYTSFAPTAGGTTIHAKFVPSFNATIVDRLEVAGAV 120 DA+IA+G + G LHGVPIG+KDLCYT APTA GTTI F P+++AT+V RL AGAV Sbjct: 72 ADADIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAV 131 Query: 121 TLGKLKMTEGAYTSHHPAIQAPLNPWNVNYWVGSSSTGSGVATSAGLCYGAIGSDTGGSI 180 +GKL MTEGAY +HP++ P+NPW+ W G SS+G GVAT+AGLC+G+IGSDTGGSI Sbjct: 132 IIGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGSI 191 Query: 181 RFPSATCGLTGIKPTWGRVSRHGVFPLADSLDHVGPMCRSAADAAAMLGVIAGADINDPT 240 RFP++ CG+TGIKPTWGRVSRHGV LA S DHVGP+ RSA DAA +L VIAG+DI+DP+ Sbjct: 192 RFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSVIAGSDIHDPS 251 Query: 241 ALRAPVPNYLAGIDDGIRGLRIGVDRRYTQEGIDRHVVAALVQAERTLADLGADIREVHF 300 PVP+Y A + R R+GVD T D A L +TL D+G + +V Sbjct: 252 CSAEPVPDYAADL-ALTRIPRVGVDWSQTTS-FDEDTTAMLADVVKTLDDIGWPVIDVKL 309 Query: 301 PPYQKLVSQWIAMCSVETAEAHLDTYPSRKSEYGPDLAQLIEQGRATSGVDIAAIHHERL 360 P +V+ + M +VETA AH DTYP+R EYGP + +I+ G + V+ + RL Sbjct: 310 PALAPMVAAFGKMRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVEYQTLTERRL 369 Query: 361 KFSGSLAAMFEDIDLLLIPTMPVPIPTLTKMSEYGADPSVLLSILRFTAVFDFSGSPTIT 420 +F+ SL +F D+D+LL+P+ + PTL M G DP + + TA F+ SG+P I Sbjct: 370 EFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPFNVSGNPAIC 429 Query: 421 LPMGMASDQMPLSMQLVGPHLSEDVLVRAGAAFQSVTDWHTRRPPI 466 LP G + PL +Q +G E +LVRAG AFQ VT +H RRPP+ Sbjct: 430 LPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV 475 >PA4483 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Pae Pseudomonas aeruginosa Length = 484 Score = 251 bits (641), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 170/489 (34%), Positives = 254/489 (51%), Gaps = 39/489 (7%) Query: 6 LHYKSITEISELIRRKEAKPSEITEAMLGRIAKLDGQFHGYALVLAERAMAQAKQYDAEI 65 LH ++ EI+ + K+ E+T +LGRI +LD Q + + + + A+AQAK D Sbjct: 2 LHQLTLAEIARALADKQFSAEELTRTLLGRIRQLDPQLNSFISITDDLAIAQAKAADERR 61 Query: 66 AKGIWRGPLHGVPIGLKDLCYTSFAPTAGGTTIHAKFVPSFNATIVDRLEVAGAVTLGKL 125 A G G L G PI KDL T T+ G+ + FV ++AT+V++L AGAVTLGKL Sbjct: 62 ANG-ENGALLGAPIAHKDLFCTQGVRTSCGSKMLDNFVSPYDATVVEKLTAAGAVTLGKL 120 Query: 126 KMTEGAY-----TSHHPAIQAPLNPWNVNYWVGSSSTGSGVATSAGLCYGAIGSDTGGSI 180 M E A +SH+ A++ NPW+++ G SS GS A +A L A G+DTGGSI Sbjct: 121 NMDEFAMGSSNQSSHYGAVK---NPWSLDRVPGGSSGGSAAAVAARLLPAATGTDTGGSI 177 Query: 181 RFPSATCGLTGIKPTWGRVSRHGVFPLADSLDHVGPMCRSAADAAAMLGVIAGADINDPT 240 R P+A LTGIKPT+GRVSR G+ A SLD GP+ R+A D A MLGV+AG D D T Sbjct: 178 RQPAALTNLTGIKPTYGRVSRWGMIAYASSLDQGGPLARTAEDCALMLGVMAGFDPKDST 237 Query: 241 ALRAPVPNYLAGIDDGIRGLRIGVDRRYTQEGIDRHVVAALVQAERTLADLGADIREVHF 300 ++ PV +YLA + + GLRIG+ R Y G+D + A++ L LGA ++++ Sbjct: 238 SVEQPVDDYLAALQKPLSGLRIGLPREYFGAGLDSRIADAVLAVVEELKTLGATVKDISL 297 Query: 301 PPYQKLVSQWIAMCSVETAEAHLDTYPSRKSEYGPDLAQLIE---------------QGR 345 P Q + + + E A ++L + + Y D Q +E + R Sbjct: 298 PNMQHAIPAYYVIAPAE-ASSNLSRFDGVRYGYRCDAPQNLEDLYKRSRAEGFGSEVKNR 356 Query: 346 ATSGV--------DIAAIHHERLK--FSGSLAAMFEDIDLLLIPTMPVPIPTLTKMSEYG 395 G D + ++++ + F ++D++L PT P P K+ E Sbjct: 357 IMVGTYALSAGYYDAYYLQAQKIRRLIKNDFVSAFAEVDVILGPTTPNPA---WKIGEKN 413 Query: 396 ADPSVLLSILRFTAVFDFSGSPTITLPMGMASDQMPLSMQLVGPHLSEDVLVRAGAAFQS 455 DP +T + +G P +++P G D +P+ +QL+ P+ E L+ +Q Sbjct: 414 DDPVSQYLEDIYTITANLAGLPGLSMPAGFV-DGLPVGVQLLAPYFQEGRLLNVAHQYQQ 472 Query: 456 VTDWHTRRP 464 V+DWHTR P Sbjct: 473 VSDWHTRTP 481 >BH0666 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Bha Bacillus halodurans Length = 485 Score = 249 bits (637), Expect = 7e-66, Method: Compositional matrix adjust. Identities = 165/488 (33%), Positives = 251/488 (51%), Gaps = 30/488 (6%) Query: 5 SLHYKSITEISELIRRKEAKPSEITEAMLGRIAKLDGQFHGYALVLAERAMAQAKQYDAE 64 SL + ++ + KE S++ + RI ++DGQ + + E+A A AK+ DA Sbjct: 2 SLFDLKLKDVHTKLHEKEISVSDLVDEAYKRIEQVDGQVEAFLALNEEKARAYAKELDAA 61 Query: 65 IAKGIWRGPLHGVPIGLKDLCYTSFAPTAGGTTIHAKFVPSFNATIVDRLEVAGAVTLGK 124 + + RG L G+PIG+KD T T + I F P ++AT+V +L A AVT+GK Sbjct: 62 LDRSEARGLLFGIPIGVKDNIVTKNLRTTCSSRILGNFDPIYDATVVHKLREAQAVTIGK 121 Query: 125 LKMTEGAY--TSHHPAIQAPLNPWNVNYWVGSSSTGSGVATSAGLCYGAIGSDTGGSIRF 182 L M E A ++ + A Q NPWN+ Y G SS GS A +AG +GSDTGGSIR Sbjct: 122 LNMDEFAMGSSTENSAFQKTKNPWNLEYVPGGSSGGSAAAVAAGEVPFTLGSDTGGSIRQ 181 Query: 183 PSATCGLTGIKPTWGRVSRHGVFPLADSLDHVGPMCRSAADAAAMLGVIAGADINDPTAL 242 P+A CG+ G+KPT+GRVSR+G+ A SLD +GP+ R+ D A +L I+G D D T+ Sbjct: 182 PAAYCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRNVEDNAYLLQAISGHDPMDSTSA 241 Query: 243 RAPVPNYLAGIDDGIRGLRIGVDRRYTQEGIDRHVVAALVQAERTLADLGADIREVHFPP 302 VP+YL+ + I+GL+I V + Y EG+ V +++ A + L LGA EV P Sbjct: 242 NLDVPDYLSALTGDIKGLKIAVPKEYLGEGVKEEVKQSVLDALKVLEGLGATWEEVSLPH 301 Query: 303 YQKLVSQWIAMCSVE------------------TAEAHLDTYPSRKSE-YGPDLAQLIEQ 343 + ++ + + S E A+ LD Y ++E +G ++ + I Sbjct: 302 SKYALATYYLLASSEASANLARFDGVRYGFRSDNADNLLDMYKQTRAEGFGDEVKRRIML 361 Query: 344 GR--ATSGVDIA---AIHHERLKFSGSLAAMFEDIDLLLIPTMPVPIPTLTKMSEYGADP 398 G +SG A R +FE D+++ PT P P K+ E DP Sbjct: 362 GTFALSSGYYDAYYKKAQQVRTLIKQDFEKVFEQYDVIIGPTTPTPA---FKIGEKTDDP 418 Query: 399 SVLLSILRFTAVFDFSGSPTITLPMGMASDQMPLSMQLVGPHLSEDVLVRAGAAFQSVTD 458 + + T + +G P I++P G + +PL +Q++G H E + R AF+ TD Sbjct: 419 LTMYANDILTIPVNLAGVPAISVPCGF-DNGLPLGLQIIGKHFDEGSVYRVAHAFEQATD 477 Query: 459 WHTRRPPI 466 +HT+RP + Sbjct: 478 YHTKRPTL 485 >BS_yerM [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Bsu Bacillus subtilis Length = 485 Score = 242 bits (617), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 164/488 (33%), Positives = 255/488 (52%), Gaps = 30/488 (6%) Query: 5 SLHYKSITEISELIRRKEAKPSEITEAMLGRIAKLDGQFHGYALVLAERAMAQAKQYDAE 64 SL ITE+ +LI +KE K S++ + RI +D + + + ERA A AK+ D Sbjct: 2 SLFDHKITELKQLIHKKEIKISDLVDESYKRIQAVDDKVQAFLALDEERARAYAKELDEA 61 Query: 65 IAKGIWRGPLHGVPIGLKDLCYTSFAPTAGGTTIHAKFVPSFNATIVDRLEVAGAVTLGK 124 + G L G+PIG+KD T T + I F P ++AT+V RL+ A AVT+GK Sbjct: 62 VDGRSEHGLLFGMPIGVKDNIVTKGLRTTCSSKILENFDPIYDATVVQRLQDAEAVTIGK 121 Query: 125 LKMTEGAY--TSHHPAIQAPLNPWNVNYWVGSSSTGSGVATSAGLCYGAIGSDTGGSIRF 182 L M E A ++ + A + NPWN++ G SS GS A +AG ++GSDTGGSIR Sbjct: 122 LNMDEFAMGSSTENSAYKLTKNPWNLDTVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIRQ 181 Query: 183 PSATCGLTGIKPTWGRVSRHGVFPLADSLDHVGPMCRSAADAAAMLGVIAGADINDPTAL 242 P++ CG+ G+KPT+GRVSR+G+ A SLD +GP+ R+ D A +L I+G D D T+ Sbjct: 182 PASFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAFLLQAISGVDKMDSTSA 241 Query: 243 RAPVPNYLAGIDDGIRGLRIGVDRRYTQEGIDRHVVAALVQAERTLADLGADIREVHFPP 302 VP++L+ + I+GL+I V + Y EG+ + +++ A + L LGA EV P Sbjct: 242 NVDVPDFLSSLTGDIKGLKIAVPKEYLGEGVGKEARESVLAALKVLEGLGATWEEVSLPH 301 Query: 303 YQKLVSQWIAMCSVE------------------TAEAHLDTYPSRKSE-YGPDLAQLIEQ 343 + ++ + + S E A+ +D Y ++E +G ++ + I Sbjct: 302 SKYALATYYLLSSSEASANLARFDGIRYGYRTDNADNLIDLYKQTRAEGFGNEVKRRIML 361 Query: 344 GR-ATSGVDIAAIHHERLKFSGSLAAMFEDI----DLLLIPTMPVPIPTLTKMSEYGADP 398 G A S A + + K + FED+ D+++ PT P P K+ E DP Sbjct: 362 GTFALSSGYYDAYYKKAQKVRTLIKKDFEDVFEKYDVIVGPTTPTPA---FKIGENTKDP 418 Query: 399 SVLLSILRFTAVFDFSGSPTITLPMGMASDQMPLSMQLVGPHLSEDVLVRAGAAFQSVTD 458 + + T + +G P I++P G+A D +PL +Q++G H E + R AF+ TD Sbjct: 419 LTMYANDILTIPVNLAGVPGISVPCGLA-DGLPLGLQIIGKHFDESTVYRVAHAFEQATD 477 Query: 459 WHTRRPPI 466 H +P + Sbjct: 478 HHKAKPEL 485