BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0099.p (345 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3366 [R] COG0388 Predicted amidohydrolase Pae Pseudomonas aeru... 572 e-163 HP0294 [R] COG0388 Predicted amidohydrolase Hpy Helicobacter pyl... 560 e-159 jhp0279 [R] COG0388 Predicted amidohydrolase jHp Helicobacter py... 557 e-158 HP1238 [R] COG0388 Predicted amidohydrolase Hpy Helicobacter pyl... 206 6e-53 jhp1159 [R] COG0388 Predicted amidohydrolase jHp Helicobacter py... 201 2e-51 >PA3366 [R] COG0388 Predicted amidohydrolase Pae Pseudomonas aeruginosa Length = 346 Score = 572 bits (1474), Expect = e-163, Method: Compositional matrix adjust. Identities = 264/338 (78%), Positives = 295/338 (87%) Query: 1 MLHGDISSSKDTVGVAVVNYKMPRLHTKAEVLDNARNIAKMVEGMKLGLPGMDLVIFPEY 60 M HGDISSS DTVGVAVVNYKMPRLHT AEVLDNAR IA+M+ GMK GLPGMDLV+FPEY Sbjct: 1 MRHGDISSSNDTVGVAVVNYKMPRLHTAAEVLDNARKIAEMIVGMKQGLPGMDLVVFPEY 60 Query: 61 STHGIMYDSKEMYETASSVPGEETRIFADACRKAKVWGVFSLTGERHEEHPNKAPYNTLI 120 S GIMYD EM ETA ++PGEET IF+ ACRKA VWGVFSLTGERHEEHP KAPYNTL+ Sbjct: 61 SLQGIMYDPAEMMETAVAIPGEETEIFSRACRKANVWGVFSLTGERHEEHPRKAPYNTLV 120 Query: 121 LMNDKGEIVQKYRKIMPWVPIEGWYPGDCTYVSEGPKGLKISLIICDDGNYPEIWRDCAM 180 L+++ GEIVQKYRKI+PW PIEGWYPG TYVSEGPKG+KISLIICDDGNYPEIWRDCAM Sbjct: 121 LIDNNGEIVQKYRKIIPWCPIEGWYPGGQTYVSEGPKGMKISLIICDDGNYPEIWRDCAM 180 Query: 181 RGAELIVRCQGYMYPAKEQQIQISKSMAWANNCYVAVANASGFDGVYSYFGHSALIGFDG 240 +GAELIVRCQGYMYPAK+QQ+ ++K+MAWANNCYVAVANA+GFDGVYSYFGHSA+IGFDG Sbjct: 181 KGAELIVRCQGYMYPAKDQQVMMAKAMAWANNCYVAVANAAGFDGVYSYFGHSAIIGFDG 240 Query: 241 RTLGECGTEENGIQYAQLSVSLIRDARRNMQSQNHLFKLMHRGYTGLINSGDGDRGHAVC 300 RTLGECG EE GIQYAQLS+S IRDAR N QSQNHLFK++HRGY+GL SGDGDRG A C Sbjct: 241 RTLGECGEEEMGIQYAQLSLSQIRDARANDQSQNHLFKILHRGYSGLQASGDGDRGLAEC 300 Query: 301 PYTFYKNWITDPEGTREMVEAMTRPTVGTDECPIEGIP 338 P+ FY+ W+TD E RE VE +TR T G +CP+ +P Sbjct: 301 PFEFYRTWVTDAEKARENVERLTRSTTGVAQCPVGRLP 338 >HP0294 [R] COG0388 Predicted amidohydrolase Hpy Helicobacter pylori 26695 Length = 339 Score = 560 bits (1442), Expect = e-159, Method: Compositional matrix adjust. Identities = 259/340 (76%), Positives = 296/340 (87%), Gaps = 1/340 (0%) Query: 1 MLHGDISSSKDTVGVAVVNYKMPRLHTKAEVLDNARNIAKMVEGMKLGLPGMDLVIFPEY 60 M HGDISSS DTVGVAVVNYKMPRLHTK EVL+N RNIAK++ G+K GLPG+DL+IFPEY Sbjct: 1 MRHGDISSSPDTVGVAVVNYKMPRLHTKNEVLENCRNIAKVIGGVKQGLPGLDLIIFPEY 60 Query: 61 STHGIMYDSKEMYETASSVPGEETRIFADACRKAKVWGVFSLTGERHEEHPNKAPYNTLI 120 STHGIMYD +EM++TA+SVPGEET IFA+AC+K KVWGVFSLTGE+HE+ K PYNTLI Sbjct: 61 STHGIMYDRQEMFDTAASVPGEETAIFAEACKKNKVWGVFSLTGEKHEQ-AKKNPYNTLI 119 Query: 121 LMNDKGEIVQKYRKIMPWVPIEGWYPGDCTYVSEGPKGLKISLIICDDGNYPEIWRDCAM 180 L+NDKGEIVQKYRKI+PW PIE WYPGD TYV +GPKGLK+SLIICDDGNYPEIWRDCAM Sbjct: 120 LVNDKGEIVQKYRKILPWCPIECWYPGDKTYVVDGPKGLKVSLIICDDGNYPEIWRDCAM 179 Query: 181 RGAELIVRCQGYMYPAKEQQIQISKSMAWANNCYVAVANASGFDGVYSYFGHSALIGFDG 240 RGAELIVRCQGYMYPAKEQQI I K+MAWAN CYVAVANA+GFDGVYSYFGHS++IGFDG Sbjct: 180 RGAELIVRCQGYMYPAKEQQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDG 239 Query: 241 RTLGECGTEENGIQYAQLSVSLIRDARRNMQSQNHLFKLMHRGYTGLINSGDGDRGHAVC 300 TLGECG EENG+QYAQLSV IRDAR+ QSQN LFKL+HRGY+G+ SGDGD+G A C Sbjct: 240 HTLGECGEEENGLQYAQLSVQQIRDARKYDQSQNQLFKLLHRGYSGVFASGDGDKGVAEC 299 Query: 301 PYTFYKNWITDPEGTREMVEAMTRPTVGTDECPIEGIPNQ 340 P+ FYK W+ DP+ +E VE +TRP+VG CP+ +P + Sbjct: 300 PFEFYKTWVNDPKKAQENVEKITRPSVGVAACPVGDLPTK 339 >jhp0279 [R] COG0388 Predicted amidohydrolase jHp Helicobacter pylori J99 Length = 339 Score = 557 bits (1435), Expect = e-158, Method: Compositional matrix adjust. Identities = 258/340 (75%), Positives = 294/340 (86%), Gaps = 1/340 (0%) Query: 1 MLHGDISSSKDTVGVAVVNYKMPRLHTKAEVLDNARNIAKMVEGMKLGLPGMDLVIFPEY 60 M HGDISSS DTVGVAVVNYKMPRLHTK EVL+N RNIAK++ G+K GLPG+DL+IFPEY Sbjct: 1 MRHGDISSSPDTVGVAVVNYKMPRLHTKNEVLENCRNIAKVIGGVKQGLPGLDLIIFPEY 60 Query: 61 STHGIMYDSKEMYETASSVPGEETRIFADACRKAKVWGVFSLTGERHEEHPNKAPYNTLI 120 STHGIMYD +EM++TA+SVPGEET I A+AC+K KVWGVFSLTGE+HE+ K PYNTLI Sbjct: 61 STHGIMYDRQEMFDTAASVPGEETAILAEACKKNKVWGVFSLTGEKHEQ-AKKNPYNTLI 119 Query: 121 LMNDKGEIVQKYRKIMPWVPIEGWYPGDCTYVSEGPKGLKISLIICDDGNYPEIWRDCAM 180 L+NDKGEIVQKYRKI+PW PIE WYPGD TYV +GPKGLK+SLIICDDGNYPEIWRDCAM Sbjct: 120 LVNDKGEIVQKYRKILPWCPIECWYPGDKTYVVDGPKGLKVSLIICDDGNYPEIWRDCAM 179 Query: 181 RGAELIVRCQGYMYPAKEQQIQISKSMAWANNCYVAVANASGFDGVYSYFGHSALIGFDG 240 RGAELIVRCQGYMYPAKEQQI I K+MAWAN CYVAVANA+GFDGVYSYFGHS++IGFDG Sbjct: 180 RGAELIVRCQGYMYPAKEQQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDG 239 Query: 241 RTLGECGTEENGIQYAQLSVSLIRDARRNMQSQNHLFKLMHRGYTGLINSGDGDRGHAVC 300 TLGECG EENG+QYAQLSV IRDAR+ QSQN LFKL+HRGY+G+ SGDGD+G A C Sbjct: 240 HTLGECGEEENGLQYAQLSVQQIRDARKYDQSQNQLFKLLHRGYSGVFASGDGDKGVAEC 299 Query: 301 PYTFYKNWITDPEGTREMVEAMTRPTVGTDECPIEGIPNQ 340 P+ FYK W+ DP+ +E VE TRP+VG CP+ +P + Sbjct: 300 PFEFYKTWVNDPKKAQENVEKFTRPSVGVAACPVGDLPTK 339 >HP1238 [R] COG0388 Predicted amidohydrolase Hpy Helicobacter pylori 26695 Length = 334 Score = 206 bits (525), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 106/272 (38%), Positives = 162/272 (59%), Gaps = 3/272 (1%) Query: 15 VAVVNYKMPRLHTKAEVLDNARNIAKMVEGMKLGLPGMDLVIFPEYSTHGIMYDSKEMYE 74 VA + + +P ++++ ++ N +I + + K G PG++L+IFPEYST G+ E Sbjct: 16 VAAIQFPVPIVNSRKDIDHNIESIIRTLHATKAGYPGVELIIFPEYSTQGLNTAKWLSEE 75 Query: 75 TASSVPGEETRIFADACRKAKVWGVFSLTGERHEEHPNKAPYNTLILMNDKGEIVQKYRK 134 VPG+ET ++A AC++AKV+GVFS+ ER+ + NK PYNT I+++ +GEI+ KYRK Sbjct: 76 FLLDVPGKETELYAKACKEAKVYGVFSIM-ERNPDS-NKNPYNTAIIIDPQGEIILKYRK 133 Query: 135 IMPWVPIEGWYPGDCTY-VSEGPKGLKISLIICDDGNYPEIWRDCAMRGAELIVRCQGYM 193 + PW PIE WYPGD V EGP G K+++ IC DG PE+ R+ A +G + +R GY Sbjct: 134 LFPWNPIEPWYPGDLGMPVCEGPGGSKLAVCICHDGMIPELAREAAYKGCNVYIRISGYS 193 Query: 194 YPAKEQQIQISKSMAWANNCYVAVANASGFDGVYSYFGHSALIGFDGRTLGECGTEENGI 253 +Q I ++S AW N Y N +G+D V+ YFG + FDG TL + I Sbjct: 194 TQVNDQWILTNRSNAWHNLMYTVSVNLAGYDNVFYYFGEGQICNFDGTTLVQGHRNPWEI 253 Query: 254 QYAQLSVSLIRDARRNMQSQNHLFKLMHRGYT 285 ++ + +AR + +N+++ L HRGY Sbjct: 254 VTGEIYPKMADNARLSWGLENNIYNLGHRGYV 285 >jhp1159 [R] COG0388 Predicted amidohydrolase jHp Helicobacter pylori J99 Length = 334 Score = 201 bits (511), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 103/272 (37%), Positives = 161/272 (59%), Gaps = 3/272 (1%) Query: 15 VAVVNYKMPRLHTKAEVLDNARNIAKMVEGMKLGLPGMDLVIFPEYSTHGIMYDSKEMYE 74 VA + + +P ++++ ++ N +I + + K G PG++L+IFPEYST G+ E Sbjct: 16 VAAIQFPVPIVNSRKDIDHNIESIIRTLHATKAGYPGVELIIFPEYSTQGLNTAKWLSEE 75 Query: 75 TASSVPGEETRIFADACRKAKVWGVFSLTGERHEEHPNKAPYNTLILMNDKGEIVQKYRK 134 VPG+ET ++A AC++AKV+GV S+ ER+ + N+ PYNT I+++ +G+I+ KYRK Sbjct: 76 FLLDVPGKETELYAKACKEAKVYGVLSIM-ERNPDS-NENPYNTAIIIDPQGKIILKYRK 133 Query: 135 IMPWVPIEGWYPGDCTY-VSEGPKGLKISLIICDDGNYPEIWRDCAMRGAELIVRCQGYM 193 + PW PIE WYPGD V EGP G K+++ IC DG PE+ R+ A +G + +R GY Sbjct: 134 LFPWNPIEPWYPGDLGMPVCEGPGGSKLAVCICHDGMIPELAREAAYKGCNVYIRISGYS 193 Query: 194 YPAKEQQIQISKSMAWANNCYVAVANASGFDGVYSYFGHSALIGFDGRTLGECGTEENGI 253 +Q I ++S AW N Y N +G+D V+ YFG + FDG TL + I Sbjct: 194 TQVNDQWILTNRSNAWHNLMYTVSVNLAGYDNVFYYFGEGQICNFDGTTLVQGHRNPWEI 253 Query: 254 QYAQLSVSLIRDARRNMQSQNHLFKLMHRGYT 285 ++ + +AR + +N+++ L HRGY Sbjct: 254 VTGEIYPKMADNARLSWGLENNIYNLGHRGYV 285