BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0060.p (243 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc03191 [H] COG2243 Precorrin-2 methylase Sme Sinorhizobium mel... 224 2e-58 mlr1380 Non COG 221 1e-57 AGc5080 [H] COG2243 Precorrin-2 methylase Atu Agrobacterium tume... 209 3e-54 PA2904 [H] COG2243 Precorrin-2 methylase Pae Pseudomonas aeruginosa 184 1e-46 BMEI0713 [H] COG2243 Precorrin-2 methylase Bme Brucella melitensis 180 2e-45 >SMc03191 [H] COG2243 Precorrin-2 methylase Sme Sinorhizobium meliloti Length = 251 Score = 224 bits (570), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 120/234 (51%), Positives = 150/234 (64%), Gaps = 1/234 (0%) Query: 2 GRIICCGLGPGDPDLMSVRADRMVRAAKHVAYFRKKGQPGQARRIVEGLLAADVSEYPME 61 GR+I G GPGDP+L++V+A R + A VAYF K G+ G R +VEGLL ++E P+ Sbjct: 7 GRLIGVGTGPGDPELLTVKAVRALGEADVVAYFAKAGRNGNGRAVVEGLLKPGMTELPLY 66 Query: 62 YPVTTEIAFDSPDYARLLADFYDEWAERLA-RFARAIDIVVLCEGDPYFYGSFMHLHARL 120 YPVTTEI D Y R + DFYD AE +A + VL EGDP FYGS+MHLH RL Sbjct: 67 YPVTTEIDKDHDAYKRQITDFYDASAEAVAAHLLAGRTVAVLSEGDPLFYGSYMHLHVRL 126 Query: 121 QGRVEIEVIAGIPGMVGCWNSVSQPIALADDVTTVLMGTLPEPELAERMRSSDALVVMKT 180 GR EVI GI M GCW+ P+ DDV +VL GT+ E EL R+ + A V+MK Sbjct: 127 AGRFPAEVIPGITAMSGCWSLAGLPLVQGDDVLSVLPGTMGEGELRRRLADTQAAVIMKV 186 Query: 181 GRNLPKIGRALASAGRLDDAWLVERGTMPDQRVARLADIGEAECPYFAIVLVHG 234 GRNLPKI RALA+AGRL+ A VERGTM + + L + + E PYF++VLV G Sbjct: 187 GRNLPKIRRALAAAGRLEAAVYVERGTMRNAAMVPLGEKPDDEAPYFSLVLVPG 240 >mlr1380 Non COG Length = 256 Score = 221 bits (563), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 118/238 (49%), Positives = 147/238 (61%), Gaps = 1/238 (0%) Query: 2 GRIICCGLGPGDPDLMSVRADRMVRAAKHVAYFRKKGQPGQARRIVEGLLAADVSEYPME 61 G+++ G GPGDP+L++++A R + A VAYF K+G AR IVE D+ E P+ Sbjct: 11 GKLVGVGTGPGDPELLTLKAARALAEADVVAYFAKRGNNSNARAIVEARFRPDMLELPLL 70 Query: 62 YPVTTEIAFDSPDYARLLADFYDEWAERLARFARAIDIV-VLCEGDPYFYGSFMHLHARL 120 YPVTTEI D DY + FY+E A+++A A +V VL EGDP FYGS+MHLH RL Sbjct: 71 YPVTTEIDKDHDDYRSQITGFYEESAQKVAEHLEAGKMVAVLSEGDPLFYGSYMHLHVRL 130 Query: 121 QGRVEIEVIAGIPGMVGCWNSVSQPIALADDVTTVLMGTLPEPELAERMRSSDALVVMKT 180 R EVI GI M GCW+ PI DDV TVL GT+ E EL R+ +DA V+MK Sbjct: 131 AHRFPTEVIPGITAMSGCWSQTGVPIVQGDDVLTVLPGTMSEFELTRRLADTDAAVIMKV 190 Query: 181 GRNLPKIGRALASAGRLDDAWLVERGTMPDQRVARLADIGEAECPYFAIVLVHGQGRR 238 GRNLPKI RAL G+L A VERGTM +LAD + + PYFAI+LV G R Sbjct: 191 GRNLPKIRRALEVTGKLTRAVYVERGTMAGSVSTKLADKRDDKAPYFAIILVAGWSGR 248 >AGc5080 [H] COG2243 Precorrin-2 methylase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 255 Score = 209 bits (533), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 108/238 (45%), Positives = 146/238 (61%), Gaps = 1/238 (0%) Query: 2 GRIICCGLGPGDPDLMSVRADRMVRAAKHVAYFRKKGQPGQARRIVEGLLAADVSEYPME 61 G+++ G GPGDP+L++++A R + A +A+F KKG G R IVE + E P+ Sbjct: 11 GKLVGVGTGPGDPELLTLKAVRAIENADVLAFFCKKGSAGNGRGIVEAFIRPGTLEMPLV 70 Query: 62 YPVTTEIAFDSPDYARLLADFYDEWAERLA-RFARAIDIVVLCEGDPYFYGSFMHLHARL 120 YPVT E + DY +A F+D+ A+ +A ++ VL EGDP FYGS+MHLH RL Sbjct: 71 YPVTVESDKNGEDYRGAIAAFFDQSAKDIAVHLDAGRNVAVLSEGDPLFYGSYMHLHLRL 130 Query: 121 QGRVEIEVIAGIPGMVGCWNSVSQPIALADDVTTVLMGTLPEPELAERMRSSDALVVMKT 180 E EV+AGI M GCW+ P+ DD+ +VL GTL E LAER+ +D V+MK Sbjct: 131 APSYEAEVVAGITAMSGCWSMAGLPLVQGDDILSVLPGTLAEEVLAERLSGTDGAVIMKV 190 Query: 181 GRNLPKIGRALASAGRLDDAWLVERGTMPDQRVARLADIGEAECPYFAIVLVHGQGRR 238 GRNLPKI RAL AG+L++A VERGTM + RL + + PYF++VLV G R Sbjct: 191 GRNLPKIRRALEVAGKLEEALYVERGTMANSHAVRLIERDASPAPYFSLVLVPGWKTR 248 >PA2904 [H] COG2243 Precorrin-2 methylase Pae Pseudomonas aeruginosa Length = 250 Score = 184 bits (467), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 101/234 (43%), Positives = 140/234 (59%), Gaps = 1/234 (0%) Query: 2 GRIICCGLGPGDPDLMSVRADRMVRAAKHVAYFRKKGQPGQARRIVEGLLAADVSEYPME 61 GR++ G+GPGDP+L++++A R++RAA V YF KG+ G A I+E L P+ Sbjct: 7 GRLLGLGVGPGDPELITLKALRLLRAAPVVGYFVAKGKKGNAFGIIEAHLDQAQVRLPLV 66 Query: 62 YPVTTEIAFDSPDYARLLADFYDEWAERLARFARA-IDIVVLCEGDPYFYGSFMHLHARL 120 YPVTTE Y +++DFYD AE+LA A D+ V+CEGDP+FYGS+M+LH RL Sbjct: 67 YPVTTEKLEPPLCYETIISDFYDTAAEQLAAHLDAGRDVAVICEGDPFFYGSYMYLHDRL 126 Query: 121 QGRVEIEVIAGIPGMVGCWNSVSQPIALADDVTTVLMGTLPEPELAERMRSSDALVVMKT 180 R E EV+ G+ M+G + P+ + +VL G LPE EL R+ +DA VVMK Sbjct: 127 ATRYESEVVPGVCSMLGGAAVLGAPLVYRNQSLSVLSGVLPEEELRRRLADADAAVVMKL 186 Query: 181 GRNLPKIGRALASAGRLDDAWLVERGTMPDQRVARLADIGEAECPYFAIVLVHG 234 GRN K+ R L G A VER TM +QR+ L + PYF++++V G Sbjct: 187 GRNFDKVRRVLVELGLERRALYVERATMANQRIVPLERVEPMASPYFSLIVVPG 240 >BMEI0713 [H] COG2243 Precorrin-2 methylase Bme Brucella melitensis Length = 244 Score = 180 bits (457), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 89/235 (37%), Positives = 143/235 (60%), Gaps = 1/235 (0%) Query: 2 GRIICCGLGPGDPDLMSVRADRMVRAAKHVAYFRKKGQPGQARRIVEGLLAADVSEYPME 61 GR+ G+GPGDP+L++++A R++++A VAY +G+ G A IVE L+ + P+ Sbjct: 5 GRLFGLGVGPGDPELITLKALRLLKSAPVVAYHAARGKKGNAFTIVEDYLSPEQVLLPLI 64 Query: 62 YPVTTEIAFDSPDYARLLADFYDEWAERLA-RFARAIDIVVLCEGDPYFYGSFMHLHARL 120 YPVTTE D DY +++ DFY E +A + D+ V+ EGDP+FYGSFM++H RL Sbjct: 65 YPVTTEKLPDHMDYEQVVKDFYGEITTTIASHLDQGQDVAVIAEGDPFFYGSFMYIHDRL 124 Query: 121 QGRVEIEVIAGIPGMVGCWNSVSQPIALADDVTTVLMGTLPEPELAERMRSSDALVVMKT 180 + E V+ G+ ++G + P+ + +VL G + EL +R+ ++A +MK Sbjct: 125 AEKYETHVVPGVCSVLGASAVLGAPLVYRNQTLSVLSGVMSANELRQRLAQTEAAAIMKL 184 Query: 181 GRNLPKIGRALASAGRLDDAWLVERGTMPDQRVARLADIGEAECPYFAIVLVHGQ 235 G+NL K+ L G + A VER TM +QR+ LA++ ++CPYF+++LV G+ Sbjct: 185 GKNLDKVRAVLGDLGLMGRALYVERATMANQRIVPLAEVNGSDCPYFSLILVPGE 239