BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0049.p (222 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEII0246 [C] COG0778 Nitroreductase Bme Brucella melitensis 170 2e-42 AGc3047 [C] COG0778 Nitroreductase Atu Agrobacterium tumefaciens... 145 5e-35 SMc00166 [C] COG0778 Nitroreductase Sme Sinorhizobium meliloti 141 1e-33 SSO2560 [C] COG0778 Nitroreductase Sso Sulfolobus solfataricus 139 4e-33 mll7468 [C] COG0778 Nitroreductase Mlo Mesorhizobium loti 127 2e-29 >BMEII0246 [C] COG0778 Nitroreductase Bme Brucella melitensis Length = 227 Score = 170 bits (430), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 86/200 (43%), Positives = 120/200 (60%) Query: 14 VSFDDAFRRQLHELFVWRRDVRRFRADPLPDGTIERLIEIACLSPSVGLSQPWRFVIVDD 73 + F + R EL WRRDVR FR DP+P+ ++ L +PSVG ++PWR V+ Sbjct: 21 MQFSSSERETFFELIKWRRDVRHFRRDPVPEDMVQALKTAMDHAPSVGNARPWRIFSVES 80 Query: 74 ASRRRAVIEDFRACNADALRSYSGERAAKYAALKLSGLEEAPGHLAVFAEKTDLIGAGLG 133 ++R+AV F N A + Y G RA++Y ALKL G+E AP LAVF + G GLG Sbjct: 81 QAKRKAVHHIFTEANQAAAQIYDGARASQYHALKLEGIETAPVQLAVFTDTAPAEGHGLG 140 Query: 134 RATMPETSEYSVVAAICSMWLAARAEGIGIGWVSILNPERIHDVLEIPHSWKFIGYLCIG 193 R +M T E S A+ ++ LAAR+ G+G G VS+L+P+ + + E+P SW+F YLCIG Sbjct: 141 RQSMASTLEQSTAMAVQNLNLAARSFGLGAGMVSVLDPQAMEQLFEVPPSWRFTLYLCIG 200 Query: 194 YPQTECDRPELEQAKWESRR 213 +P TE D P L + W+ R Sbjct: 201 WPCTEDDTPLLHRNGWQENR 220 >AGc3047 [C] COG0778 Nitroreductase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 238 Score = 145 bits (367), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 86/220 (39%), Positives = 117/220 (53%), Gaps = 2/220 (0%) Query: 3 SKIDPAKGPAMVSFDDAFRRQLHELFVWRRDVR-RFRADPLPDGTIERLIEIACLSPSVG 61 + D A PA F A R ++ RRDVR +F DPLP+ +ERL++ A +PSVG Sbjct: 14 NPFDHALSPAR-PFSGAEREAIYRAIETRRDVRDQFLPDPLPEELVERLLKAAHSAPSVG 72 Query: 62 LSQPWRFVIVDDASRRRAVIEDFRACNADALRSYSGERAAKYAALKLSGLEEAPGHLAVF 121 QPW F +V D R+A F N +A + GE+ A Y +LKL G+ +AP + V Sbjct: 73 FMQPWNFTLVTDGGVRQAAWVAFSRANEEAAAMFEGEKQALYRSLKLEGIRKAPLSICVT 132 Query: 122 AEKTDLIGAGLGRATMPETSEYSVVAAICSMWLAARAEGIGIGWVSILNPERIHDVLEIP 181 + T LGR P T YS V AI ++WLAARAEGIG+GWVSI + + +L+IP Sbjct: 133 CDPTRGGKVVLGRTHNPRTDVYSTVCAIQNLWLAARAEGIGVGWVSIFHDSDVRAILDIP 192 Query: 182 HSWKFIGYLCIGYPQTECDRPELEQAKWESRRGPDEFTTR 221 + + +LC+G PEL W R +E R Sbjct: 193 EHIEIVAWLCLGRVDALYTEPELAVKGWRQRVPLEELVFR 232 >SMc00166 [C] COG0778 Nitroreductase Sme Sinorhizobium meliloti Length = 227 Score = 141 bits (355), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 76/183 (41%), Positives = 104/183 (56%), Gaps = 1/183 (0%) Query: 31 RRDVR-RFRADPLPDGTIERLIEIACLSPSVGLSQPWRFVIVDDASRRRAVIEDFRACNA 89 RRDVR F +PL + I RL+ A +PSVG QPW FV+V R V + F+ N Sbjct: 30 RRDVRDEFLPEPLSEELIARLLGAAHQAPSVGFMQPWNFVLVRQDETREKVWQAFQRAND 89 Query: 90 DALRSYSGERAAKYAALKLSGLEEAPGHLAVFAEKTDLIGAGLGRATMPETSEYSVVAAI 149 +A +SGER AKY +LKL G+ +AP + V ++T LGR P+ YS V A+ Sbjct: 90 EAAEMFSGERQAKYRSLKLEGIRKAPLSICVTCDRTRGGAVVLGRTHNPQMDLYSTVCAV 149 Query: 150 CSMWLAARAEGIGIGWVSILNPERIHDVLEIPHSWKFIGYLCIGYPQTECDRPELEQAKW 209 ++WLAARAEG+G+GWVSI + I +L IP + + +LC+G+ PEL W Sbjct: 150 QNLWLAARAEGVGVGWVSIFHESEIKAILGIPDHVEIVAWLCLGFVDRLYQEPELAAKGW 209 Query: 210 ESR 212 R Sbjct: 210 RQR 212 >SSO2560 [C] COG0778 Nitroreductase Sso Sulfolobus solfataricus Length = 227 Score = 139 bits (351), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 73/186 (39%), Positives = 112/186 (60%), Gaps = 7/186 (3%) Query: 31 RRDVRRF-RADPLPDGTIERLIEIACLSPSVGLSQPWRFVIV-DDASRRR--AVIEDFRA 86 RRD+R + + DP+PD + +++ A ++PSVG SQPW F+I+ DD R++ V+E+ R Sbjct: 10 RRDIRSYYKPDPIPDEVLAKILSAAHMAPSVGYSQPWNFIIIRDDEIRKKIKQVVEEERK 69 Query: 87 CNADALRSYSGERAAKYAALKLSGLEEAPGHLAVFAEKTDLIGAGLGRATMPETSEYSVV 146 L ER ++ +K+ + + P ++AV + LGR+TMPETS YS V Sbjct: 70 RFESVL---DEERKLIFSKIKVEAILDTPVNMAVTYDPNRFGPNILGRSTMPETSLYSTV 126 Query: 147 AAICSMWLAARAEGIGIGWVSILNPERIHDVLEIPHSWKFIGYLCIGYPQTECDRPELEQ 206 AI ++WLAA AEGIG+G+VS N E++ ++L IP + + YL +GY + PELE+ Sbjct: 127 LAIGNLWLAATAEGIGVGFVSFFNKEKVKEILNIPKEIELVSYLTLGYVREFPKIPELEE 186 Query: 207 AKWESR 212 W R Sbjct: 187 KGWNRR 192 >mll7468 [C] COG0778 Nitroreductase Mlo Mesorhizobium loti Length = 293 Score = 127 bits (319), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 77/204 (37%), Positives = 107/204 (52%), Gaps = 1/204 (0%) Query: 10 GPAMVSFDDAFRRQLHELFVWRRDVR-RFRADPLPDGTIERLIEIACLSPSVGLSQPWRF 68 P FD R ++ RRDVR F D L D + RL+ A +PSVG QPW F Sbjct: 3 APVGDGFDQMARDAVYRAMFTRRDVRSHFLPDALDDAVLARLLLAAHHAPSVGFMQPWNF 62 Query: 69 VIVDDASRRRAVIEDFRACNADALRSYSGERAAKYAALKLSGLEEAPGHLAVFAEKTDLI 128 +++ +A+RR V + F A L ER A Y LKL G+ E+ ++ + ++ Sbjct: 63 IVIRNAARRAEVRDLFLAAREQELPEIDVERRALYRKLKLEGICESALNICITCDRERSK 122 Query: 129 GAGLGRATMPETSEYSVVAAICSMWLAARAEGIGIGWVSILNPERIHDVLEIPHSWKFIG 188 + LGR PE YS V A+ + WLAARAEG+G+GWVSI+ P+ + +L IP I Sbjct: 123 NSPLGRWHNPEMDLYSTVCAVQNFWLAARAEGVGVGWVSIIEPQALKSLLAIPEHVTPIA 182 Query: 189 YLCIGYPQTECDRPELEQAKWESR 212 YLC+G +P+LE W R Sbjct: 183 YLCVGRVSEFAPKPDLETHGWGRR 206