BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0009.p (258 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value APE2520 [Q] COG3473 Maleate cis-trans isomerase Ape Aeropyrum pe... 106 6e-23 PH1150 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus ho... 98 2e-20 PAB2420 [Q] COG3473 Maleate cis-trans isomerase Pab Pyrococcus a... 92 9e-19 PH0985 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus ho... 91 2e-18 SSO2939 [Q] COG3473 Maleate cis-trans isomerase Sso Sulfolobus s... 86 1e-16 >APE2520 [Q] COG3473 Maleate cis-trans isomerase Ape Aeropyrum pernix Length = 272 Score = 106 bits (264), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 71/221 (32%), Positives = 112/221 (50%), Gaps = 6/221 (2%) Query: 6 RLGMLTPSSNTALEPITCAMLSGIDDVSAHFSRFKVTEIALSEQALRQFDASEILRAAEL 65 R+G++ PSSNT +EP M + VS H R + ++++ E+ L+ + S I AE Sbjct: 42 RIGLIVPSSNTTMEPEMWMMAP--EGVSIHTGRVPLEKVSV-EELLKMEEYSAI--EAEK 96 Query: 66 LAHAKVDVIAWNGTSASWLGFD-RDESLCEEIMAATGIKACTTVLAYRDLLRRIGASRIG 124 LA A VD+I + T+ S +G D + +++ +A+G++ TT A + L+ +GASRI Sbjct: 97 LATAGVDLIVYGCTTGSLIGGPGYDLKIADKLSSASGVQVVTTATAVVEALKALGASRIA 156 Query: 125 LVTPYRKDVQERIIANWNSQGLHCFAERHLSLQDNFSFAEVREAEIARLIEEVVREGCDA 184 + TPY ++V +R G + L L N V + RL + EG DA Sbjct: 157 VATPYIEEVNKREEEFLRHHGFDIVDFKSLGLLGNLDIGRVPPWRVYRLARSLSIEGADA 216 Query: 185 AVVLCTNMRGAGAAERLERRFGVPVLDSIAVTLWACLTAAG 225 + CTN+R LER G+PV+ S ++W L G Sbjct: 217 IFISCTNLRTVEVIGLLERDTGLPVVSSNTASMWMALKTLG 257 >PH1150 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus horikoshii Length = 236 Score = 98.2 bits (243), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 69/217 (31%), Positives = 110/217 (50%), Gaps = 6/217 (2%) Query: 6 RLGMLTPSSNTALEPITCAMLSGIDDVSAHFSRFKVTEIALSEQALRQFDASEILRAAEL 65 R+G++ PSSNT +E + L + VS H SR + I +++ L + ++ I AA L Sbjct: 7 RIGLIVPSSNTTMEMELHSFLP--EGVSLHTSRMPLKSI--TDEELFKMNSYAI-EAASL 61 Query: 66 LAHAKVDVIAWNGTSASWLG-FDRDESLCEEIMAATGIKACTTVLAYRDLLRRIGASRIG 124 LA A V+VIA+ +S S++G D ++ L +I I+ TT A + L + A + Sbjct: 62 LADAGVEVIAFGCSSGSFIGGQDFEKELEMKIEEEVNIETFTTSTAVLEALDVLDAQSLL 121 Query: 125 LVTPYRKDVQERIIANWNSQGLHCFAERHLSLQDNFSFAEVREAEIARLIEEVVREGCDA 184 +VTPY ++ ++ G + L+L DN ++ + RL + + DA Sbjct: 122 IVTPYSDEINQKEKEFLEGNGFEVLDIKGLNLTDNLEIGKLEPYVVYRLAKAMFAGDADA 181 Query: 185 AVVLCTNMRGAGAAERLERRFGVPVLDSIAVTLWACL 221 + CTN+R E+LER GVPV+ S TLW L Sbjct: 182 IFISCTNLRTFEIIEKLERDLGVPVITSNQATLWMAL 218 >PAB2420 [Q] COG3473 Maleate cis-trans isomerase Pab Pyrococcus abyssi Length = 236 Score = 92.4 bits (228), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 67/217 (30%), Positives = 107/217 (49%), Gaps = 6/217 (2%) Query: 6 RLGMLTPSSNTALEPITCAMLSGIDDVSAHFSRFKVTEIALSEQALRQFDASEILRAAEL 65 R+G++ PSSNT +E ++L + VS H SR + +++E+ L +S + AA+L Sbjct: 7 RIGLIVPSSNTTMEMEFHSVLP--EGVSLHTSRMPLK--SVTEEELLNM-SSYAIEAAKL 61 Query: 66 LAHAKVDVIAWNGTSASWLG-FDRDESLCEEIMAATGIKACTTVLAYRDLLRRIGASRIG 124 LA A V+VIA+ TS S++G D ++ L +I ++ TT A + L + + Sbjct: 62 LADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTVQ 121 Query: 125 LVTPYRKDVQERIIANWNSQGLHCFAERHLSLQDNFSFAEVREAEIARLIEEVVREGCDA 184 ++TPY ++ +R + G R L + DN ++ RL + D Sbjct: 122 VITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVDG 181 Query: 185 AVVLCTNMRGAGAAERLERRFGVPVLDSIAVTLWACL 221 + CTN R E+LER GVPV+ S TLW L Sbjct: 182 IFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMAL 218 >PH0985 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus horikoshii Length = 238 Score = 90.9 bits (224), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 69/234 (29%), Positives = 109/234 (46%), Gaps = 7/234 (2%) Query: 6 RLGMLTPSSNTALEPITCAMLSGIDDVSAHFSRFKVTEIALSEQALRQFDASEILRAAEL 65 R+G++ PSSNT +EP M + VS H +R ++ E+ +E++L + + A EL Sbjct: 7 RIGLIVPSSNTTMEPEFWKMAP--EGVSIHTARMRLREV--TEESLLEMERHAKDAALEL 62 Query: 66 LAHAKVDVIAWNGTSASWL-GFDRDESLCEEIMAATGIKACTTVLAYRDLLRRIGASRIG 124 A A+VDVI + TS S + G D+ + + + A+GIK TT A + L + ++ Sbjct: 63 -ADAEVDVIVYGCTSGSLIKGKGYDKEIAKNLEEASGIKTITTSTAVLNALNTLDIQKVV 121 Query: 125 LVTPYRKDVQERIIANWNSQGLHCFAERHLSLQDNFSFAEVREAEIARLIEEVVREGCDA 184 + TPY V ER + G+ + L + N +L EV D Sbjct: 122 VATPYIDSVNEREKEFLEANGIEVLRIKGLGIVKNTEIGRQEPWVAYKLALEVYTPEADG 181 Query: 185 AVVLCTNMRGAGAAERLERRFGVPVLDSIAVTLWACLTAAGCVPSRVQGWGSLF 238 + CTN R ++LE GVPV+ S T+W L V + +G+L Sbjct: 182 LFISCTNFRTIEIIDKLEVELGVPVVTSNQATMWYALKTMK-VKEKYDMYGTLL 234 >SSO2939 [Q] COG3473 Maleate cis-trans isomerase Sso Sulfolobus solfataricus Length = 242 Score = 85.5 bits (210), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 62/240 (25%), Positives = 113/240 (47%), Gaps = 10/240 (4%) Query: 5 VRLGMLTPSSNTALEPITCAMLSGIDD--VSAHFSRFKVTEIALSEQALRQFDASEILRA 62 +++G++ PSSNT +E + + I + ++ HFSR K+ + + + A + E RA Sbjct: 4 IKVGVIIPSSNTTVEYEFSNVFNQIKEYRITPHFSRIKLRNVTIEDLAEME---RETERA 60 Query: 63 AELLAHAKVDVIAWNGTSAS-WLGFDRDESLCEEIMAATGIKACTTVLAYRDLLRRIGAS 121 A L+ +I++ T+ S + G E + + I + A T + + LRR+ Sbjct: 61 ASELSTILPQIISYACTTGSLFKGPKHHEEIIQRIEKIAKVPATATSGSVINALRRLDIK 120 Query: 122 RIGLVTPYRKDVQERIIANWNSQGLHCFAERHLSLQDNFSFAEVREAEIARLIEEVVRE- 180 R+ L+TPY ++V ++ I ++ G + +++N +V E+ V+R Sbjct: 121 RLVLLTPYIEEVNKKEIEFFSKNGFEIVKSAGMGIRENIKIGQVTPEEVYNFAVNVLRNV 180 Query: 181 --GCDAAVVLCTNMRGAGAAERLERRFGVPVLDSIAVTLWACLTAAGCVPSRVQGWGSLF 238 D + CTN+R RLE+ PV+ S + TLW L + +V+ G LF Sbjct: 181 KGDYDGVFISCTNLRTFEILSRLEKETKKPVISSNSATLWETLYKIN-LNIKVRELGHLF 239